Reviewed, UniProtKB/Swiss-Prot Q945B7 (CRD1_EUPES)

Names and origin

Protein names

Recommended name:
    Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase, chloroplastic
      Short name=Mg-protoporphyrin IX monomethyl ester oxidative cyclase
    EC=1.14.13.81

Gene names

Name:	CRD1
Synonyms:	AT103

Organism

Euphorbia esula (Leafy spurge)

Taxonomic identifier

3993 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Malpighiales › Euphorbiaceae › Euphorbioideae › Euphorbieae › Euphorbia

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) By similarity.
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O. 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + 2 H2O. 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = divinylprotochlorophyllide + NADP+ + 2 H2O.
Cofactor	Iron By similarity.
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis.
Subcellular location	Plastid › chloroplast By similarity.
Sequence similarities	Belongs to the acsF family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 44	44	Chloroplast Potential
	Chain	45 – 405	361	Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase, chloroplastic		PRO_0000000601

Sequences

	Sequence		Length	Mass (Da)
	Q945B7-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: A0011A0E4B58372C	FASTA	405	47,310
	10 20 30 40 50 60 MAAEMALVKP ITPKFINPMR TFSSSSKFST IKMSATSQSN TTTTATKPSK KGNKKEINET 70 80 90 100 110 120 LLTPRFYTTD FDEMETLFNT EINKKLNQSE FEALLQEFKT DYNQTHFVRN KEFKEAADKM 130 140 150 160 170 180 QGPLRQIFVE FLERSCTAEF SGFLLYKELG RRLKKTNPVV AEIFSLMSRD EARHAGFLNK 190 200 210 220 230 240 GLSDFNYALD LGFLTKARKY TFFKPKFIFY ATYLSEKIGY WRYITIYRHL KENPEYQCYP 250 260 270 280 290 300 IFKYFENWCQ DENRHGDFFS ALMKAQPQFL NDWKAKLWAR FFCLSVYVTM YLNDCQRTAF 310 320 330 340 350 360 YEGIGLDTKE FDMHVIIETN RTTARIFPAV LDVENPEFKR KLDRMVVINQ KLQAVGETED 370 380 390 400 NSVVKNLKRV PLIAALVSEI LAAYLMPPIE SGSVDFAEFE PKLVY

References

[1]	"Novel regulation and promoter analysis of a gene with similarity to the leucine zipper-containing gene At103." Horvath D.P. Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF417577 Genomic DNA. Translation: AAL13304.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.13.81. 289405.
Family and domain databases
InterPro	IPR008434. AcsF. IPR003251. Rubrerythrin. [Graphical view]
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CRD1_EUPES

Accession

Primary (citable) accession number: Q945B7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	December 1, 2001
Last modified:	June 16, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)