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Protein

Splicing factor U2af 38 kDa subunit

Gene

U2af38

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Necessary for the splicing of pre-mRNA. Binds to the polypyrimidine tract of introns early during spliceosome assembly (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri12 – 4029C3H1-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri151 – 17828C3H1-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • mRNA binding Source: FlyBase
  • nucleotide binding Source: InterPro
  • poly-pyrimidine tract binding Source: FlyBase

GO - Biological processi

  • mitotic spindle organization Source: FlyBase
  • mRNA splicing, via spliceosome Source: FlyBase
  • regulation of alternative mRNA splicing, via spliceosome Source: FlyBase
  • regulation of glucose metabolic process Source: FlyBase
  • RNA splicing Source: FlyBase
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-109688. Cleavage of Growing Transcript in the Termination Region.
R-DME-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-DME-72163. mRNA Splicing - Major Pathway.
R-DME-72187. mRNA 3'-end processing.

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing factor U2af 38 kDa subunit
Alternative name(s):
U2 auxiliary factor 38 kDa subunit
U2 snRNP auxiliary factor small subunit
Gene namesi
Name:U2af38
ORF Names:CG3582
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0017457. U2af38.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: FlyBase
  • precatalytic spliceosome Source: FlyBase
  • spliceosomal complex Source: FlyBase
  • U2AF Source: FlyBase
  • U2 snRNP Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 264264Splicing factor U2af 38 kDa subunitPRO_0000081997Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei19 – 191Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ94535.

PTM databases

iPTMnetiQ94535.

Expressioni

Gene expression databases

BgeeiQ94535.
ExpressionAtlasiQ94535. differential.
GenevisibleiQ94535. DM.

Interactioni

Subunit structurei

Associates with a 65 kDa protein.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
U2af50Q245624EBI-121011,EBI-165226

Protein-protein interaction databases

BioGridi59457. 4 interactions.
DIPiDIP-23299N.
IntActiQ94535. 4 interactions.
MINTiMINT-311934.
STRINGi7227.FBpp0305600.

Structurei

3D structure databases

ProteinModelPortaliQ94535.
SMRiQ94535. Positions 15-193.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini44 – 149106RRMPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi180 – 21334Arg/Ser-rich (RS domain)Add
BLAST
Compositional biasi190 – 1978Poly-Arg
Compositional biasi252 – 26211Poly-GlyAdd
BLAST

Sequence similaritiesi

Belongs to the splicing factor SR family.Curated
Contains 2 C3H1-type zinc fingers.PROSITE-ProRule annotation
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri12 – 4029C3H1-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri151 – 17828C3H1-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG2202. Eukaryota.
ENOG410Z5PX. LUCA.
GeneTreeiENSGT00530000063193.
InParanoidiQ94535.
KOiK12836.
OMAiKMRGRDE.
OrthoDBiEOG70089G.
PhylomeDBiQ94535.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR003954. RRM_dom_euk.
IPR009145. U2AF_small.
IPR000571. Znf_CCCH.
[Graphical view]
PANTHERiPTHR12620. PTHR12620. 1 hit.
PfamiPF00076. RRM_1. 1 hit.
PF00642. zf-CCCH. 2 hits.
[Graphical view]
PRINTSiPR01848. U2AUXFACTOR.
SMARTiSM00361. RRM_1. 1 hit.
SM00356. ZnF_C3H1. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
PS50103. ZF_C3H1. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q94535-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEYLASIFG TEKDKVNCSF YFKIGACRHG DRCSRIHNKP TFSQTVLLQN
60 70 80 90 100
LYVNPQNSAK SADGSHLVAN VSDEEMQEHY DNFFEDVFVE CEDKYGEIEE
110 120 130 140 150
MNVCDNLGDH LVGNVYIKFR NEADAEKAAN DLNNRWFGGR PVYSELSPVT
160 170 180 190 200
DFREACCRQY EMGECTRSGF CNFMHLKPIS RELRRYLYSR RRRARSRSRS
210 220 230 240 250
PGRRRGSRSR SRSPGRRGGG RGDGVGGGNY LNNERDNMRG NDRGNDRDRR
260
KGGGGGGGGG GGRY
Length:264
Mass (Da):29,877
Last modified:June 1, 2001 - v2
Checksum:i577285FB66FDB2F5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti66 – 661H → D in AAB17271 (PubMed:8816800).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U67066 mRNA. Translation: AAB17271.1.
AE014134 Genomic DNA. Translation: AAF51512.1.
AY058537 mRNA. Translation: AAL13766.1.
PIRiJC6125.
RefSeqiNP_001259814.1. NM_001272885.1.
NP_477208.1. NM_057860.4.
UniGeneiDm.4117.

Genome annotation databases

EnsemblMetazoaiFBtr0078133; FBpp0077792; FBgn0017457.
FBtr0333408; FBpp0305600; FBgn0017457.
GeneIDi33201.
KEGGidme:Dmel_CG3582.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U67066 mRNA. Translation: AAB17271.1.
AE014134 Genomic DNA. Translation: AAF51512.1.
AY058537 mRNA. Translation: AAL13766.1.
PIRiJC6125.
RefSeqiNP_001259814.1. NM_001272885.1.
NP_477208.1. NM_057860.4.
UniGeneiDm.4117.

3D structure databases

ProteinModelPortaliQ94535.
SMRiQ94535. Positions 15-193.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi59457. 4 interactions.
DIPiDIP-23299N.
IntActiQ94535. 4 interactions.
MINTiMINT-311934.
STRINGi7227.FBpp0305600.

PTM databases

iPTMnetiQ94535.

Proteomic databases

PaxDbiQ94535.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0078133; FBpp0077792; FBgn0017457.
FBtr0333408; FBpp0305600; FBgn0017457.
GeneIDi33201.
KEGGidme:Dmel_CG3582.

Organism-specific databases

CTDi33201.
FlyBaseiFBgn0017457. U2af38.

Phylogenomic databases

eggNOGiKOG2202. Eukaryota.
ENOG410Z5PX. LUCA.
GeneTreeiENSGT00530000063193.
InParanoidiQ94535.
KOiK12836.
OMAiKMRGRDE.
OrthoDBiEOG70089G.
PhylomeDBiQ94535.

Enzyme and pathway databases

ReactomeiR-DME-109688. Cleavage of Growing Transcript in the Termination Region.
R-DME-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-DME-72163. mRNA Splicing - Major Pathway.
R-DME-72187. mRNA 3'-end processing.

Miscellaneous databases

ChiTaRSiU2af38. fly.
GenomeRNAii33201.
PROiQ94535.

Gene expression databases

BgeeiQ94535.
ExpressionAtlasiQ94535. differential.
GenevisibleiQ94535. DM.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR003954. RRM_dom_euk.
IPR009145. U2AF_small.
IPR000571. Znf_CCCH.
[Graphical view]
PANTHERiPTHR12620. PTHR12620. 1 hit.
PfamiPF00076. RRM_1. 1 hit.
PF00642. zf-CCCH. 2 hits.
[Graphical view]
PRINTSiPR01848. U2AUXFACTOR.
SMARTiSM00361. RRM_1. 1 hit.
SM00356. ZnF_C3H1. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
PS50103. ZF_C3H1. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mutations in the small subunit of the Drosophila U2AF splicing factor cause lethality and developmental defects."
    Rudner D.Z., Kanaar R., Breger K.S., Rio D.C.
    Proc. Natl. Acad. Sci. U.S.A. 93:10333-10337(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Embryo.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. "A Drosophila full-length cDNA resource."
    Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
    Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  5. "Phosphoproteome analysis of Drosophila melanogaster embryos."
    Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
    J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.

Entry informationi

Entry nameiU2AF1_DROME
AccessioniPrimary (citable) accession number: Q94535
Secondary accession number(s): Q9VPN4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 1, 2001
Last modified: June 8, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.