Q94524 (DYLT_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dynein light chain Tctex-type

Alternative name(s):
TCTEX-1 protein homolog

Gene names

Name:	Dlc90F
Synonyms:	Tctex
ORF Names:	CG12363

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	111 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as one of several non-catalytic accessory components of the cytoplasmic dynein complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Required for spermatid differentiation. Is not required for polarized transport in rhabdomere development and appears to be a non-essential component of the cytoplasmic dynein complex. Ref.6
Subunit structure	The cytoplasmic dynein complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs) By similarity. Ref.6
Subcellular location	Cytoplasm › cytoskeleton Probable.
Disruption phenotype	Male sterility. Ref.6
Sequence similarities	Belongs to the dynein light chain Tctex-type family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Dynein Microtubule
Molecular function	Motor protein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mitosis Inferred from mutant phenotype. Source: FlyBase spermatid development Inferred from mutant phenotype Ref.6. Source: FlyBase
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW dynein complex Inferred from electronic annotation. Source: UniProtKB-KW microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dynein intermediate chain binding Inferred from physical interaction. Source: FlyBase motor activity Inferred from electronic annotation. Source: UniProtKB-KW protein homodimerization activity Inferred from direct assay. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 111

111

Dynein light chain Tctex-type

PRO_0000195155

Secondary structure

1

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111

Helix Strand Turn

Details...

Helix

14 – 27

14

Helix

34 – 53

20

Beta strand

58 – 69

12

Beta strand

75 – 83

9

Turn

85 – 87

3

Beta strand

89 – 96

8

Beta strand

98 – 110

13

Sequences

	Sequence		Length	Mass (Da)
	Q94524 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 544CC39F5B318137	FASTA	111	12,479
	10 20 30 40 50 60 MDDSREESQF IVDDVSKTIK EAIETTIGGN AYQHDKVNNW TGQVVENCLT VLTKEQKPYK 70 80 90 100 110 YIVTAMIMQK NGAGLHTASS CYWNNDTDGS CTVRWENKTM YCIVSVFGLA V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of nuclear genes encoding mitochondrial proteins: isolation of a collection of D. melanogaster cDNAs homologous to sequences in the Human Gene Index database." Caggese C., Ragone G., Perrini B., Moschetti R., de Pinto V., Caizzi R., Barsanti P. Mol. Gen. Genet. 261:64-70(1999) [PubMed: 10071211] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary.
[2]	"dtctex-1, the Drosophila melanogaster homolog of a putative murine t-complex distorter encoding a dynein light chain, is required for production of functional sperm." Caggese C., Moschetti R., Ragone G., Barsanti P., Caizzi R. Mol. Genet. Genomics 265:436-444(2001) [PubMed: 11405626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[6]	"The Drosophila tctex-1 light chain is dispensable for essential cytoplasmic dynein functions but is required during spermatid differentiation." Li M.G., Serr M., Newman E.A., Hays T.S. Mol. Biol. Cell 15:3005-3014(2004) [PubMed: 15090621] [Abstract] Cited for: SUBUNIT, FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y08968 mRNA. Translation: CAA70165.1.
AF123058 Genomic DNA. Translation: AAD30033.1.
AE014297 Genomic DNA. Translation: AAF55532.1.
AY069608 mRNA. Translation: AAL39753.1.

RefSeq

NP_477356.1. NM_058008.3.

UniGene

Dm.2045.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YGT	X-ray	1.70	A	1-111	[»]
2PG1	X-ray	2.80	E/F/G/H	1-111	[»]
3FM7	X-ray	3.50	A/B	1-111	[»]

ProteinModelPortal

Q94524.

SMR

Q94524. Positions 8-111.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-17339N.

IntAct

Q94524. 11 interactions.

MINT

MINT-281764.

STRING

Q94524.

Proteomic databases

PRIDE

Q94524.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblMetazoa

FBtr0083575; FBpp0082996; FBgn0024432.

GeneID

42199.

KEGG

dme:Dmel_CG12363.

NMPDR

fig|7227.3.peg.13430.

Organism-specific databases

CTD

42199.

FlyBase

FBgn0024432. Dlc90F.

Phylogenomic databases

eggNOG

inNOG10427.

GeneTree

EMGT00050000013896.

InParanoid

Q94524.

OMA

SCFWDNS.

OrthoDB

EOG4KH1BJ.

PhylomeDB

Q94524.

Gene expression databases

Bgee

Q94524.

GermOnline

CG12363. Drosophila melanogaster.

Family and domain databases

InterPro

IPR005334. Tctex.
[Graphical view]

KO

K10420.

PANTHER

PTHR21255. Tctex. 1 hit.

Pfam

PF03645. Tctex-1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

827633.

Entry information

Entry name

DYLT_DROME

Accession

Primary (citable) accession number: Q94524
Secondary accession number(s): Q9VE95

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project