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Protein

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

Gene

SdhA

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Maintaining electron transport chain function is required to prevent neurodegenerative changes seen in both early- and late-onset disorders.1 Publication

Catalytic activityi

Succinate + a quinone = fumarate + a quinol.By similarity

Cofactori

FADBy similarity

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes fumarate from succinate (eukaryal route).By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SdhB), Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SdhBL), Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SdhBL), Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SdhA), Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SdhAL)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes fumarate from succinate (eukaryal route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei272FADBy similarity1
Binding sitei293SubstrateBy similarity1
Binding sitei305SubstrateBy similarity1
Active sitei337Proton acceptorBy similarity1
Binding sitei404SubstrateBy similarity1
Binding sitei438FADBy similarity1
Binding sitei449SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi65 – 70FADBy similarity6
Nucleotide bindingi88 – 103FADBy similarityAdd BLAST16
Nucleotide bindingi454 – 455FADBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Transport, Tricarboxylic acid cycle
LigandFAD, Flavoprotein

Enzyme and pathway databases

ReactomeiR-DME-71403 Citric acid cycle (TCA cycle)
UniPathwayiUPA00223; UER01006

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (EC:1.3.5.1By similarity)
Alternative name(s):
Flavoprotein subunit of complex II
Short name:
FP
Succinyl coenzyme A synthetase flavoprotein subunit
Gene namesi
Name:SdhA
Synonyms:Scs-fp
ORF Names:CG17246
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0261439 SdhA

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Disruption phenotypei

Disruption of mitochondrial function has no effect on the initial stages of photoreceptor development (R cells develop normally, adopt the correct cell fates, innervate the appropriate synaptic partners, and assemble synapses normally). However, beginning around the time of eclosion, R cells degenerate, progressively losing expression of synaptic markers and undergoing extensive morphological changes. Synapse loss is caused by reactive oxygen species (ROS) production, not energy depletion, as photoreceptor ATP levels are normal.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 41MitochondrionSequence analysisAdd BLAST41
ChainiPRO_000001034042 – 661Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrialAdd BLAST620

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei96Tele-8alpha-FAD histidineBy similarity1

Proteomic databases

PaxDbiQ94523
PRIDEiQ94523

Expressioni

Gene expression databases

BgeeiFBgn0261439
GenevisibleiQ94523 DM

Interactioni

Subunit structurei

Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit.By similarity

Protein-protein interaction databases

BioGridi62892, 13 interactors
DIPiDIP-19386N
IntActiQ94523, 3 interactors
STRINGi7227.FBpp0085736

Structurei

3D structure databases

ProteinModelPortaliQ94523
SMRiQ94523
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2403 Eukaryota
COG1053 LUCA
GeneTreeiENSGT00910000144277
InParanoidiQ94523
KOiK00234
OMAiGDSPWEH
OrthoDBiEOG091G041Z
PhylomeDBiQ94523

Family and domain databases

Gene3Di3.50.50.60, 2 hits
3.90.700.10, 1 hit
InterProiView protein in InterPro
IPR003953 FAD-binding_2
IPR036188 FAD/NAD-bd_sf
IPR003952 FRD_SDH_FAD_BS
IPR037099 Fum_R/Succ_DH_flav-like_C_sf
IPR015939 Fum_Rdtase/Succ_DH_flav-like_C
IPR027477 Succ_DH/fumarate_Rdtase_cat_sf
IPR011281 Succ_DH_flav_su_fwd
IPR014006 Succ_Dhase_FrdA_Gneg
PfamiView protein in Pfam
PF00890 FAD_binding_2, 1 hit
PF02910 Succ_DH_flav_C, 1 hit
SUPFAMiSSF46977 SSF46977, 1 hit
SSF51905 SSF51905, 2 hits
SSF56425 SSF56425, 1 hit
TIGRFAMsiTIGR01816 sdhA_forward, 1 hit
TIGR01812 sdhA_frdA_Gneg, 1 hit
PROSITEiView protein in PROSITE
PS00504 FRD_SDH_FAD_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q94523-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGIMRVPSI LAKNAVASMQ RAAAVGVQRS YHITHGRQQA SAANPDKISK
60 70 80 90 100
QYPVVDHAYD AIVVGAGGAG LRAAFGLVAE GFRTAVITKL FPTRSHTIAA
110 120 130 140 150
QGGINAALGN MEEDDWKWHM YDTVKGSDWL GDQDAIHYMT REAPKAVIEL
160 170 180 190 200
ENYGMPFSRT QDGKIYQRAF GGQSLKFGKG GQAHRCCAVA DRTGHSLLHT
210 220 230 240 250
LYGQSLSYDC NYFVEYFALD LIFEDGECRG VLALNLEDGT LHRFRAKNTV
260 270 280 290 300
IATGGYGRAF FSCTSAHTCT GDGTAMVARQ GLPSQDLEFV QFHPTGIYGA
310 320 330 340 350
GCLITEGCRG EGGYLINGNG ERFMERYAPV AKDLASRDVV SRSMTIEIME
360 370 380 390 400
GRGAGPEKDH VYLQLHHLPP KQLAERLPGI SETAMIFAGV DVTREPIPVL
410 420 430 440 450
PTVHYNMGGV PTNYRGQVIT IDKDGKDVIV PGLYAAGEAA SSSVHGANRL
460 470 480 490 500
GANSLLDLVV FGRACAKTIA ELNKPGAPAP TLKENAGEAS VANLDKLRHA
510 520 530 540 550
NGQITTADLR LKMQKTMQHH AAVFRDGPIL QDGVNKMKEI YKQFKDIKVV
560 570 580 590 600
DRSLIWNSDL VETLELQNLL ANAQMTIVSA EARKESRGAH AREDFKVRED
610 620 630 640 650
EYDFSKPLDG QQKKPMDQHW RKHTLSWVCN DNGDITLDYR NVIDTTLDNE
660
VSTVPPAIRS Y
Length:661
Mass (Da):72,343
Last modified:February 21, 2001 - v3
Checksum:i301B578C7F765011
GO

Sequence cautioni

The sequence CAA70285 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti207S → N in CAA70285 (PubMed:10071211).Curated1
Sequence conflicti251I → L in CAA70285 (PubMed:10071211).Curated1
Sequence conflicti503Q → L in CAA70285 (PubMed:10071211).Curated1
Sequence conflicti520H → L in CAA70285 (PubMed:10071211).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA Translation: AAG22257.1
AE013599 Genomic DNA Translation: AAN16127.1
AY051472 mRNA Translation: AAK92896.1
BT099627 mRNA Translation: ACU51771.1
Y09064 mRNA Translation: CAA70285.1 Different initiation.
RefSeqiNP_477210.1, NM_057862.5
NP_725881.1, NM_166343.3
NP_725882.1, NM_166344.3
UniGeneiDm.4591

Genome annotation databases

EnsemblMetazoaiFBtr0086552; FBpp0085736; FBgn0261439
FBtr0086553; FBpp0085737; FBgn0261439
FBtr0086554; FBpp0085738; FBgn0261439
GeneIDi37228
KEGGidme:Dmel_CG17246

Similar proteinsi

Entry informationi

Entry nameiSDHA_DROME
AccessioniPrimary (citable) accession number: Q94523
Secondary accession number(s): A4UZP5
, C7LA77, Q0E918, Q9I7G3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 21, 2001
Last modified: May 23, 2018
This is version 175 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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