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Protein

Nascent polypeptide-associated complex subunit alpha

Gene

Nacalpha

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May promote appropriate targeting of ribosome-nascent polypeptide complexes (By similarity). Required for correct localization of the osk/oskar protein to the posterior pole during embryonic development. The osk protein directs the recruitment of molecules responsible for posterior body patterning and germline formation in the embryo.By similarity1 Publication

GO - Biological processi

  • neurogenesis Source: FlyBase
  • oogenesis Source: FlyBase
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Nascent polypeptide-associated complex subunit alpha
Short name:
NAC-alpha
Alternative name(s):
Alpha-NAC
Gene namesi
Name:Nacalpha
Synonyms:aic
ORF Names:CG8759
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0086904. Nacalpha.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 217217Nascent polypeptide-associated complex subunit alphaPRO_0000135586Add
BLAST

Proteomic databases

PaxDbiQ94518.
PRIDEiQ94518.

Expressioni

Gene expression databases

BgeeiQ94518.
GenevisibleiQ94518. DM.

Interactioni

Subunit structurei

Part of the nascent polypeptide-associated complex (NAC), consisting of Nac-alpha and bicaudal (bic).

Protein-protein interaction databases

BioGridi62181. 79 interactions.
IntActiQ94518. 18 interactions.
MINTiMINT-814952.
STRINGi7227.FBpp0086971.

Structurei

3D structure databases

ProteinModelPortaliQ94518.
SMRiQ94518. Positions 82-133.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini70 – 13566NAC-A/BPROSITE-ProRule annotationAdd
BLAST
Domaini177 – 21741UBAAdd
BLAST

Sequence similaritiesi

Belongs to the NAC-alpha family.Curated
Contains 1 NAC-A/B (NAC-alpha/beta) domain.PROSITE-ProRule annotation
Contains 1 UBA domain.Curated

Phylogenomic databases

eggNOGiKOG2239. Eukaryota.
COG1308. LUCA.
GeneTreeiENSGT00440000033468.
InParanoidiQ94518.
KOiK03626.
OMAiMANPRIE.
OrthoDBiEOG7RNK24.
PhylomeDBiQ94518.

Family and domain databases

InterProiIPR016641. EGD2/NACA.
IPR002715. Nas_poly-pep-assoc_cplx_dom.
[Graphical view]
PANTHERiPTHR21713. PTHR21713. 1 hit.
PfamiPF01849. NAC. 1 hit.
[Graphical view]
PIRSFiPIRSF015901. NAC_alpha. 1 hit.
SMARTiSM01407. NAC. 1 hit.
[Graphical view]
PROSITEiPS51151. NAC_AB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q94518-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPELTEIKSE AAPSTSAEAK PEDVRVEDDG SDSDSDGGMP GLEEAVAATT
60 70 80 90 100
QLGGGATGLP IDLVSKAKQS RGEKKARKIM LKLGLKQIQG VNRVTIRKSK
110 120 130 140 150
NILFVINNPD VYKNPHSDTY IVFGEAKIED LSQQAQVAAA EKFKAPEAAG
160 170 180 190 200
AADSVGATTS VAPIAEEDEE DVDDTGVDEK DIELVITQAN TTRAKAIKAL
210
KNNNNDIVNA IMELTML
Length:217
Mass (Da):23,021
Last modified:July 19, 2005 - v2
Checksum:i6E1BD9C91E1D7F93
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti47 – 471A → P in CAA70166 (PubMed:10071211).Curated
Sequence conflicti110 – 1101D → N in CAA70166 (PubMed:10071211).Curated
Sequence conflicti204 – 2041N → D in CAA70166 (PubMed:10071211).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08969 mRNA. Translation: CAA70166.1.
AF017783 Genomic DNA. Translation: AAB97513.1.
AE013599 Genomic DNA. Translation: AAM68653.1.
AE013599 Genomic DNA. Translation: AAM68654.1.
AY075332 mRNA. Translation: AAL68199.1.
RefSeqiNP_001286363.1. NM_001299434.1.
NP_477216.1. NM_057868.4.
NP_599139.1. NM_134312.3.
NP_725229.1. NM_165950.2.
UniGeneiDm.4630.

Genome annotation databases

EnsemblMetazoaiFBtr0087858; FBpp0086971; FBgn0086904.
FBtr0087859; FBpp0086972; FBgn0086904.
FBtr0087860; FBpp0086973; FBgn0086904.
FBtr0345195; FBpp0311394; FBgn0086904.
GeneIDi36409.
KEGGidme:Dmel_CG8759.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08969 mRNA. Translation: CAA70166.1.
AF017783 Genomic DNA. Translation: AAB97513.1.
AE013599 Genomic DNA. Translation: AAM68653.1.
AE013599 Genomic DNA. Translation: AAM68654.1.
AY075332 mRNA. Translation: AAL68199.1.
RefSeqiNP_001286363.1. NM_001299434.1.
NP_477216.1. NM_057868.4.
NP_599139.1. NM_134312.3.
NP_725229.1. NM_165950.2.
UniGeneiDm.4630.

3D structure databases

ProteinModelPortaliQ94518.
SMRiQ94518. Positions 82-133.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi62181. 79 interactions.
IntActiQ94518. 18 interactions.
MINTiMINT-814952.
STRINGi7227.FBpp0086971.

Proteomic databases

PaxDbiQ94518.
PRIDEiQ94518.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0087858; FBpp0086971; FBgn0086904.
FBtr0087859; FBpp0086972; FBgn0086904.
FBtr0087860; FBpp0086973; FBgn0086904.
FBtr0345195; FBpp0311394; FBgn0086904.
GeneIDi36409.
KEGGidme:Dmel_CG8759.

Organism-specific databases

CTDi36409.
FlyBaseiFBgn0086904. Nacalpha.

Phylogenomic databases

eggNOGiKOG2239. Eukaryota.
COG1308. LUCA.
GeneTreeiENSGT00440000033468.
InParanoidiQ94518.
KOiK03626.
OMAiMANPRIE.
OrthoDBiEOG7RNK24.
PhylomeDBiQ94518.

Miscellaneous databases

ChiTaRSiNacalpha. fly.
GenomeRNAii36409.
NextBioi798409.
PROiQ94518.

Gene expression databases

BgeeiQ94518.
GenevisibleiQ94518. DM.

Family and domain databases

InterProiIPR016641. EGD2/NACA.
IPR002715. Nas_poly-pep-assoc_cplx_dom.
[Graphical view]
PANTHERiPTHR21713. PTHR21713. 1 hit.
PfamiPF01849. NAC. 1 hit.
[Graphical view]
PIRSFiPIRSF015901. NAC_alpha. 1 hit.
SMARTiSM01407. NAC. 1 hit.
[Graphical view]
PROSITEiPS51151. NAC_AB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of nuclear genes encoding mitochondrial proteins: isolation of a collection of D. melanogaster cDNAs homologous to sequences in the Human Gene Index database."
    Caggese C., Ragone G., Perrini B., Moschetti R., de Pinto V., Caizzi R., Barsanti P.
    Mol. Gen. Genet. 261:64-70(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The oxen gene of Drosophila encodes a homolog of subunit 9 of yeast ubiquinol-cytochrome c oxidoreductase complex: evidence for modulation of gene expression in response to mitochondrial activity."
    Frolov M.V., Benevolenskaya E.V., Birchler J.A.
    Genetics 156:1727-1736(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Head.
  6. "Localization-dependent oskar protein accumulation; control after the initiation of translation."
    Braat A.K., Yan N., Arn E., Harrison D., Macdonald P.M.
    Dev. Cell 7:125-131(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH BIC.

Entry informationi

Entry nameiNACA_DROME
AccessioniPrimary (citable) accession number: Q94518
Secondary accession number(s): A4UZF1, O16813, Q0E999
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: May 11, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.