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Q94511

- NDUS1_DROME

UniProt

Q94511 - NDUS1_DROME

Protein

NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

Gene

ND75

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 3 (21 Feb 2001)
      Previous versions | rss
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    Functioni

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized By similarity.By similarity

    Catalytic activityi

    NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
    NADH + acceptor = NAD+ + reduced acceptor.

    Cofactori

    Binds 1 2Fe-2S cluster per subunit.By similarity
    Binds 2 4Fe-4S clusters per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi74 – 741Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi85 – 851Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi88 – 881Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi102 – 1021Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi134 – 1341Iron-sulfur 2 (4Fe-4S); via pros nitrogenBy similarity
    Metal bindingi138 – 1381Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi141 – 1411Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi147 – 1471Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi190 – 1901Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi193 – 1931Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi196 – 1961Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi240 – 2401Iron-sulfur 3 (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. electron carrier activity Source: InterPro
    4. metal ion binding Source: UniProtKB-KW
    5. NADH dehydrogenase (ubiquinone) activity Source: RefGenome

    GO - Biological processi

    1. ATP synthesis coupled electron transport Source: InterPro
    2. cellular respiration Source: RefGenome

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
    Alternative name(s):
    Complex I-75kD
    Short name:
    CI-75kD
    Gene namesi
    Name:ND75
    ORF Names:CG2286
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome X

    Organism-specific databases

    FlyBaseiFBgn0017566. ND75.

    Subcellular locationi

    Mitochondrion inner membrane By similarity
    Note: Matrix and cytoplasmic side of the mitochondrial inner membrane.By similarity

    GO - Cellular componenti

    1. mitochondrial respiratory chain complex I Source: RefGenome

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2727MitochondrionBy similarityAdd
    BLAST
    Chaini28 – 731704NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrialPRO_0000019971Add
    BLAST

    Proteomic databases

    PaxDbiQ94511.
    PRIDEiQ94511.

    Expressioni

    Gene expression databases

    BgeeiQ94511.

    Interactioni

    Subunit structurei

    Complex I is composed of about 45 different subunits.By similarity

    Protein-protein interaction databases

    BioGridi58223. 24 interactions.
    DIPiDIP-17348N.
    IntActiQ94511. 1 interaction.
    MINTiMINT-868919.

    Structurei

    3D structure databases

    ProteinModelPortaliQ94511.
    SMRiQ94511. Positions 42-725.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini40 – 118792Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini259 – 315574Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the complex I 75 kDa subunit family.Curated
    Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1034.
    GeneTreeiENSGT00390000018768.
    HOGENOMiHOG000264012.
    InParanoidiQ94511.
    KOiK03934.
    OMAiFQGNDVA.
    OrthoDBiEOG783MTP.
    PhylomeDBiQ94511.

    Family and domain databases

    Gene3Di3.10.20.30. 1 hit.
    InterProiIPR001041. 2Fe-2S_ferredoxin-type.
    IPR012675. Beta-grasp_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
    IPR010228. NADH_UbQ_OxRdtase_Gsu.
    IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
    IPR015405. NuoG_C.
    [Graphical view]
    PfamiPF09326. DUF1982. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF10588. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view]
    SMARTiSM00929. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF54292. SSF54292. 1 hit.
    TIGRFAMsiTIGR01973. NuoG. 1 hit.
    PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
    PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00641. COMPLEX1_75K_1. 1 hit.
    PS00642. COMPLEX1_75K_2. 1 hit.
    PS00643. COMPLEX1_75K_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q94511-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIRAPLVKAL GALGSPTHQM ASRAVRTSAM VAQTPAKAPE KIEVFVDDIP    50
    VQVVPGTTVL QAAAQIGVEI PRFCYHERLA VAGNCRMCLV EVEKSPKPVA 100
    ACAMPVMKGW RIKTNSDLTR KAREGVMEFL LMNHPLDCPI CDQGGECDLQ 150
    DQAMAFGSDR SRFTDINYTG KRAVEDKDIG PLVKTIMTRC IHCTRCVRFA 200
    SEIAGVDDLG TTGRGNDMQI GTYVEKLFLT ELSGNVIDLC PVGALTNKPY 250
    SFVARPWEIR KVSSIDVLDA VGSNIVVSTR TNEVLRILPR ENEDVNEEWL 300
    ADKSRFACDG LKRQRLVAPM VRMPNGELQA VEWEGALIAV AKAIKAAGGQ 350
    IAGISGQLAD LEAQVALKDL LNRLGSEVVA TEQGFIAGGT DNRANYLLNS 400
    TIAGLEEADA VLLVGTNPRY EAPLVNTRLR KAYVHNELQI ASIGPKIDLS 450
    YDHENLGADA ALVKDVCSGA HAFSKVLEGA KKPAIIIGAD LLERADGAAI 500
    HATVAEYCKK LKKPNWNPFN VLQTNAAQVG ALDVGYKAGA QTAVKAQPKV 550
    LFLLNADAGK VTREQLPKDC FVVYIGSHGD NGASIADAVL PGAAYTEKQG 600
    IYVNTEGRPQ QTLPGVSPPG MAREDWKILR ALSEVVGKPL PYDNLDELRN 650
    RLEDVAPHLT RLGQLEPAGD AGAAGTISKS IGGGAIDIKL KELRDYFMTD 700
    AISRASPTMA KCISAVNKQQ RENEAKQSVA I 731
    Length:731
    Mass (Da):78,630
    Last modified:February 21, 2001 - v3
    Checksum:iB3CA26B56AFACBFD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti65 – 651Q → L in CAA70284. (PubMed:10071211)Curated
    Sequence conflicti94 – 941K → N in CAA70284. (PubMed:10071211)Curated
    Sequence conflicti111 – 1111R → P in CAA70284. (PubMed:10071211)Curated
    Sequence conflicti120 – 1201R → P in CAA70284. (PubMed:10071211)Curated
    Sequence conflicti470 – 4701A → S in CAA70284. (PubMed:10071211)Curated
    Sequence conflicti611 – 6111Q → H in CAA70284. (PubMed:10071211)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014298 Genomic DNA. Translation: AAF46356.1.
    Y09063 mRNA. Translation: CAA70284.1.
    RefSeqiNP_511083.1. NM_078528.2.
    NP_727255.1. NM_167152.2.
    UniGeneiDm.6840.

    Genome annotation databases

    EnsemblMetazoaiFBtr0071180; FBpp0071128; FBgn0017566.
    FBtr0071181; FBpp0071129; FBgn0017566.
    GeneIDi31762.
    KEGGidme:Dmel_CG2286.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014298 Genomic DNA. Translation: AAF46356.1 .
    Y09063 mRNA. Translation: CAA70284.1 .
    RefSeqi NP_511083.1. NM_078528.2.
    NP_727255.1. NM_167152.2.
    UniGenei Dm.6840.

    3D structure databases

    ProteinModelPortali Q94511.
    SMRi Q94511. Positions 42-725.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 58223. 24 interactions.
    DIPi DIP-17348N.
    IntActi Q94511. 1 interaction.
    MINTi MINT-868919.

    Proteomic databases

    PaxDbi Q94511.
    PRIDEi Q94511.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0071180 ; FBpp0071128 ; FBgn0017566 .
    FBtr0071181 ; FBpp0071129 ; FBgn0017566 .
    GeneIDi 31762.
    KEGGi dme:Dmel_CG2286.

    Organism-specific databases

    CTDi 31762.
    FlyBasei FBgn0017566. ND75.

    Phylogenomic databases

    eggNOGi COG1034.
    GeneTreei ENSGT00390000018768.
    HOGENOMi HOG000264012.
    InParanoidi Q94511.
    KOi K03934.
    OMAi FQGNDVA.
    OrthoDBi EOG783MTP.
    PhylomeDBi Q94511.

    Miscellaneous databases

    GenomeRNAii 31762.
    NextBioi 775179.
    PROi Q94511.

    Gene expression databases

    Bgeei Q94511.

    Family and domain databases

    Gene3Di 3.10.20.30. 1 hit.
    InterProi IPR001041. 2Fe-2S_ferredoxin-type.
    IPR012675. Beta-grasp_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
    IPR010228. NADH_UbQ_OxRdtase_Gsu.
    IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
    IPR015405. NuoG_C.
    [Graphical view ]
    Pfami PF09326. DUF1982. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF10588. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view ]
    SMARTi SM00929. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54292. SSF54292. 1 hit.
    TIGRFAMsi TIGR01973. NuoG. 1 hit.
    PROSITEi PS51085. 2FE2S_FER_2. 1 hit.
    PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00641. COMPLEX1_75K_1. 1 hit.
    PS00642. COMPLEX1_75K_2. 1 hit.
    PS00643. COMPLEX1_75K_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    2. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    3. "Identification of nuclear genes encoding mitochondrial proteins: isolation of a collection of D. melanogaster cDNAs homologous to sequences in the Human Gene Index database."
      Caggese C., Ragone G., Perrini B., Moschetti R., de Pinto V., Caizzi R., Barsanti P.
      Mol. Gen. Genet. 261:64-70(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-653.
      Tissue: Ovary.

    Entry informationi

    Entry nameiNDUS1_DROME
    AccessioniPrimary (citable) accession number: Q94511
    Secondary accession number(s): Q9W3H1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: February 21, 2001
    Last modified: October 1, 2014
    This is version 132 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3