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Protein

NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

Gene

ND-75

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi74Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi85Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi88Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi102Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi134Iron-sulfur 2 (4Fe-4S); via tele nitrogenPROSITE-ProRule annotation1
Metal bindingi138Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi141Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi147Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi190Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi193Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi196Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi240Iron-sulfur 3 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Respiratory chain, Transport
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

ReactomeiR-DME-6799198. Complex I biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-75kD
Short name:
CI-75kD
Gene namesi
Name:ND-75Imported
Synonyms:ND75
ORF Names:CG2286Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0017566. ND-75.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 27MitochondrionBy similarityAdd BLAST27
ChainiPRO_000001997128 – 731NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrialAdd BLAST704

Proteomic databases

PaxDbiQ94511.
PRIDEiQ94511.

Expressioni

Gene expression databases

BgeeiFBgn0017566.
ExpressionAtlasiQ94511. differential.
GenevisibleiQ94511. DM.

Interactioni

Subunit structurei

Complex I is composed of about 45 different subunits.By similarity

Protein-protein interaction databases

BioGridi58223. 30 interactors.
DIPiDIP-17348N.
IntActiQ94511. 1 interactor.
MINTiMINT-868919.
STRINGi7227.FBpp0071128.

Structurei

3D structure databases

ProteinModelPortaliQ94511.
SMRiQ94511.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 1182Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST79
Domaini118 – 1574Fe-4S His(Cys)3-ligated-typePROSITE-ProRule annotationAdd BLAST40
Domaini259 – 3154Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST57

Sequence similaritiesi

Belongs to the complex I 75 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2282. Eukaryota.
COG1034. LUCA.
GeneTreeiENSGT00390000018768.
HOGENOMiHOG000264012.
InParanoidiQ94511.
KOiK03934.
OMAiPQASCAM.
OrthoDBiEOG091G02C2.
PhylomeDBiQ94511.

Family and domain databases

CDDicd00207. fer2. 1 hit.
Gene3Di3.10.20.30. 1 hit.
InterProiView protein in InterPro
IPR036010. 2Fe-2S_ferredoxin-like_sf.
IPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom_sf.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
IPR010228. NADH_UbQ_OxRdtase_Gsu.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
IPR015405. NuoG_C.
PfamiView protein in Pfam
PF00384. Molybdopterin. 1 hit.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
PF09326. NADH_dhqG_C. 1 hit.
SMARTiView protein in SMART
SM00929. NADH-G_4Fe-4S_3. 1 hit.
SUPFAMiSSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR01973. NuoG. 1 hit.
PROSITEiView protein in PROSITE
PS51085. 2FE2S_FER_2. 1 hit.
PS51839. 4FE4S_HC3. 1 hit.
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00641. COMPLEX1_75K_1. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q94511-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRAPLVKAL GALGSPTHQM ASRAVRTSAM VAQTPAKAPE KIEVFVDDIP
60 70 80 90 100
VQVVPGTTVL QAAAQIGVEI PRFCYHERLA VAGNCRMCLV EVEKSPKPVA
110 120 130 140 150
ACAMPVMKGW RIKTNSDLTR KAREGVMEFL LMNHPLDCPI CDQGGECDLQ
160 170 180 190 200
DQAMAFGSDR SRFTDINYTG KRAVEDKDIG PLVKTIMTRC IHCTRCVRFA
210 220 230 240 250
SEIAGVDDLG TTGRGNDMQI GTYVEKLFLT ELSGNVIDLC PVGALTNKPY
260 270 280 290 300
SFVARPWEIR KVSSIDVLDA VGSNIVVSTR TNEVLRILPR ENEDVNEEWL
310 320 330 340 350
ADKSRFACDG LKRQRLVAPM VRMPNGELQA VEWEGALIAV AKAIKAAGGQ
360 370 380 390 400
IAGISGQLAD LEAQVALKDL LNRLGSEVVA TEQGFIAGGT DNRANYLLNS
410 420 430 440 450
TIAGLEEADA VLLVGTNPRY EAPLVNTRLR KAYVHNELQI ASIGPKIDLS
460 470 480 490 500
YDHENLGADA ALVKDVCSGA HAFSKVLEGA KKPAIIIGAD LLERADGAAI
510 520 530 540 550
HATVAEYCKK LKKPNWNPFN VLQTNAAQVG ALDVGYKAGA QTAVKAQPKV
560 570 580 590 600
LFLLNADAGK VTREQLPKDC FVVYIGSHGD NGASIADAVL PGAAYTEKQG
610 620 630 640 650
IYVNTEGRPQ QTLPGVSPPG MAREDWKILR ALSEVVGKPL PYDNLDELRN
660 670 680 690 700
RLEDVAPHLT RLGQLEPAGD AGAAGTISKS IGGGAIDIKL KELRDYFMTD
710 720 730
AISRASPTMA KCISAVNKQQ RENEAKQSVA I
Length:731
Mass (Da):78,630
Last modified:February 21, 2001 - v3
Checksum:iB3CA26B56AFACBFD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti65Q → L in CAA70284 (PubMed:10071211).Curated1
Sequence conflicti94K → N in CAA70284 (PubMed:10071211).Curated1
Sequence conflicti111R → P in CAA70284 (PubMed:10071211).Curated1
Sequence conflicti120R → P in CAA70284 (PubMed:10071211).Curated1
Sequence conflicti470A → S in CAA70284 (PubMed:10071211).Curated1
Sequence conflicti611Q → H in CAA70284 (PubMed:10071211).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA. Translation: AAF46356.1.
Y09063 mRNA. Translation: CAA70284.1.
RefSeqiNP_511083.1. NM_078528.3.
NP_727255.1. NM_167152.2.
UniGeneiDm.6840.

Genome annotation databases

EnsemblMetazoaiFBtr0071180; FBpp0071128; FBgn0017566.
FBtr0071181; FBpp0071129; FBgn0017566.
GeneIDi31762.
KEGGidme:Dmel_CG2286.

Similar proteinsi

Entry informationi

Entry nameiNDUS1_DROME
AccessioniPrimary (citable) accession number: Q94511
Secondary accession number(s): Q9W3H1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 21, 2001
Last modified: November 22, 2017
This is version 165 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families