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Protein

Neutral alpha-glucosidase AB

Gene

modA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from N-linked oligosaccharides on newly synthesized glycoproteins.By similarity1 Publication

Catalytic activityi

Hydrolysis of terminal (1->3)-alpha-D-glucosidic links in (1->3)-alpha-D-glucans.

Pathwayi: N-glycan metabolism

This protein is involved in the pathway N-glycan metabolism, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway N-glycan metabolism and in Glycan metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei540 – 5401NucleophilePROSITE-ProRule annotation
Active sitei543 – 5431By similarity
Active sitei617 – 6171Proton donorBy similarity

GO - Molecular functioni

  • alpha-glucosidase activity Source: dictyBase
  • carbohydrate binding Source: InterPro
  • glucan 1,3-alpha-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  • N-glycan processing Source: dictyBase
  • sorocarp morphogenesis Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

ReactomeiR-DDI-901042. Calnexin/calreticulin cycle.
UniPathwayiUPA00957.

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Neutral alpha-glucosidase AB (EC:3.2.1.84)
Alternative name(s):
Alpha-glucosidase 2
Glucosidase II subunit alpha
Protein post-translational modification mutant A
Gene namesi
Name:modA
Synonyms:ganab
ORF Names:DDB_G0269154
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 1, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0269154. modA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 943920Neutral alpha-glucosidase ABPRO_0000328193Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi878 – 8781N-linked (GlcNAc...)Sequence analysis
Glycosylationi887 – 8871N-linked (GlcNAc...)Sequence analysis
Glycosylationi907 – 9071N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ94502.
PRIDEiQ94502.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0191113.

Structurei

3D structure databases

ProteinModelPortaliQ94502.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 31 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1066. Eukaryota.
COG1501. LUCA.
InParanoidiQ94502.
KOiK05546.
OMAiVNYEFAN.
PhylomeDBiQ94502.

Family and domain databases

InterProiIPR033403. DUF5110.
IPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR030458. Glyco_hydro_31_AS.
IPR025887. Glyco_hydro_31_N_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF17137. DUF5110. 1 hit.
PF13802. Gal_mutarotas_2. 1 hit.
PF01055. Glyco_hydro_31. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 2 hits.
PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q94502-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKLVILIIL SIVCSLFIGS IESVDTSKFK TCKDSHFCKR NRVSHEVGVM
60 70 80 90 100
NEMKSKQNFN IVEGSIKLVK QENTIYFDLQ EQNQKSNLLT MKLEIYEGGI
110 120 130 140 150
VRMRAQEKEP LLNKQRYQVQ DVLLDTIKTV PIQWKQEPSK QSNTFSFKHG
160 170 180 190 200
EKECCYVLVQ LVPFKLDVYI MNELAITTNS DNLFHFEPIS DKPQPLPPKE
210 220 230 240 250
KKSEEENKEA NQEEDNNNNN NDNNEEQQVS TEGYWEERFG SHQDSKPNGP
260 270 280 290 300
MSIGMDFTFV GSSHVYGIPE HTTRLSLKST TGNGINEQPY RLYNLDVFEY
310 320 330 340 350
EIDKTMALYG HVPLMISHDT KKTVGVFWLN AAETFVDIED VTTPVSPSKK
360 370 380 390 400
THWISESGII DVFYLTGPTP STIFKQYAYL TGTTALPQMF SLGYHQCKWN
410 420 430 440 450
YKSEDDVKQV DNGFDENHIP YDVIWLDIEH TDGKRYFTWD NNNFPTPADM
460 470 480 490 500
QNIIGAKHRK MVTIVDPHIK RDNNYYVHSE ATSKGYYIKN KDGNDYDGWC
510 520 530 540 550
WPGSSSYLDF TNPEIRKWWA TQFGYDKYKG STPNLYIWND MNEPSVFNGP
560 570 580 590 600
EVSMHKDAKH HGGFEHRDVH NLYGYYYHMA SADGLVQRNA DQNDRPFVLS
610 620 630 640 650
RAFYAGSQRI GAIWTGDNSA QWSHLEISNP MLLSMNLAGI TFSGADVGGF
660 670 680 690 700
FGNPDAELLT RWYQAGAFQP FFRGHAHLDS RRREPWLFNE PYTTIIREAI
710 720 730 740 750
VKRYSYLPLW YTTFYQNTLN GAPVMRPLWV QYPKEANLFD VDDHYLIGDS
760 770 780 790 800
LLVKPVTQQS CKTMKVLLPG QSVNEIWYDV DTEKPINAGV IEIDTPLEKI
810 820 830 840 850
PVYQRGGSII SKKERVRRST YQMRDDPYTI RIALDSSKSA QGQLYIDDEH
860 870 880 890 900
SFDYKKGKFL YRQFTFKDNV LSFSDASNKS STSYKPNVTI EKIVILGVQK
910 920 930 940
PHSITCNITG KEKLSFEYDS TLSKLTIRKP DLLVDTDFII KLN
Length:943
Mass (Da):108,772
Last modified:February 1, 1997 - v1
Checksum:i703DF52FDB23333E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72236 Genomic DNA. Translation: AAB18921.1.
AAFI02000005 Genomic DNA. Translation: EAL71927.1.
RefSeqiXP_646169.1. XM_641077.1.

Genome annotation databases

EnsemblProtistsiDDB0191113; DDB0191113; DDB_G0269154.
GeneIDi8617122.
KEGGiddi:DDB_G0269154.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72236 Genomic DNA. Translation: AAB18921.1.
AAFI02000005 Genomic DNA. Translation: EAL71927.1.
RefSeqiXP_646169.1. XM_641077.1.

3D structure databases

ProteinModelPortaliQ94502.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0191113.

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.

Proteomic databases

PaxDbiQ94502.
PRIDEiQ94502.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0191113; DDB0191113; DDB_G0269154.
GeneIDi8617122.
KEGGiddi:DDB_G0269154.

Organism-specific databases

dictyBaseiDDB_G0269154. modA.

Phylogenomic databases

eggNOGiKOG1066. Eukaryota.
COG1501. LUCA.
InParanoidiQ94502.
KOiK05546.
OMAiVNYEFAN.
PhylomeDBiQ94502.

Enzyme and pathway databases

UniPathwayiUPA00957.
ReactomeiR-DDI-901042. Calnexin/calreticulin cycle.

Miscellaneous databases

PROiQ94502.

Family and domain databases

InterProiIPR033403. DUF5110.
IPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR030458. Glyco_hydro_31_AS.
IPR025887. Glyco_hydro_31_N_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF17137. DUF5110. 1 hit.
PF13802. Gal_mutarotas_2. 1 hit.
PF01055. Glyco_hydro_31. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 2 hits.
PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGANAB_DICDI
AccessioniPrimary (citable) accession number: Q94502
Secondary accession number(s): Q55DG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: February 1, 1997
Last modified: May 11, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.