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Protein

S-adenosylmethionine synthase

Gene

SAMS

Organism
Dendrobium crumenatum (Tropical pigeon orchid)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.By similarity

Catalytic activityi

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Mn2+By similarity, Mg2+By similarity, Co2+By similarityNote: Binds 2 divalent ions per subunit. The metal ions interact primarily with the substrate (By similarity). Can utilize magnesium, manganese or cobalt (in vitro) (By similarity).By similarity
  • K+By similarityNote: Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate (By similarity).By similarity

Pathwayi: S-adenosyl-L-methionine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. S-adenosylmethionine synthase (SAMS)
This subpathway is part of the pathway S-adenosyl-L-methionine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine, the pathway S-adenosyl-L-methionine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi12MagnesiumBy similarity1
Binding sitei18ATPBy similarity1
Metal bindingi46PotassiumBy similarity1
Binding sitei59MethionineBy similarity1
Binding sitei102MethionineBy similarity1
Binding sitei249ATPBy similarity1
Binding sitei249Methionine; shared with neighboring subunitBy similarity1
Binding sitei272ATP; via amide nitrogen; shared with neighboring subunitBy similarity1
Binding sitei276ATP; shared with neighboring subunitBy similarity1
Binding sitei280ATP; shared with neighboring subunitBy similarity1
Binding sitei280MethionineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi170 – 172ATPBy similarity3
Nucleotide bindingi238 – 241ATPBy similarity4
Nucleotide bindingi255 – 256ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processOne-carbon metabolism
LigandATP-binding, Cobalt, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

UniPathwayiUPA00315; UER00080

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine synthase (EC:2.5.1.6By similarity)
Short name:
AdoMet synthase
Alternative name(s):
Methionine adenosyltransferase
Short name:
MAT
Gene namesi
Name:SAMS
OrganismiDendrobium crumenatum (Tropical pigeon orchid)
Taxonomic identifieri51096 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaAsparagalesOrchidaceaeEpidendroideaeMalaxideaeDendrobiinaeDendrobium

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003630211 – 395S-adenosylmethionine synthaseAdd BLAST395

Proteomic databases

PRIDEiQ944U4

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ944U4
SMRiQ944U4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AdoMet synthase family.Curated

Family and domain databases

HAMAPiMF_00086 S_AdoMet_synth1, 1 hit
InterProiView protein in InterPro
IPR022631 ADOMET_SYNTHASE_CS
IPR022630 S-AdoMet_synt_C
IPR022629 S-AdoMet_synt_central
IPR022628 S-AdoMet_synt_N
IPR002133 S-AdoMet_synthetase
IPR022636 S-AdoMet_synthetase_sfam
PANTHERiPTHR11964 PTHR11964, 1 hit
PfamiView protein in Pfam
PF02773 S-AdoMet_synt_C, 1 hit
PF02772 S-AdoMet_synt_M, 1 hit
PF00438 S-AdoMet_synt_N, 1 hit
PIRSFiPIRSF000497 MAT, 1 hit
SUPFAMiSSF55973 SSF55973, 3 hits
TIGRFAMsiTIGR01034 metK, 1 hit
PROSITEiView protein in PROSITE
PS00376 ADOMET_SYNTHASE_1, 1 hit
PS00377 ADOMET_SYNTHASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q944U4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEVDTFLFT SESVNEGHPD KLCDQISDAI LDACLEQDPD SKVACETCSK
60 70 80 90 100
TNMVMIFGEI TTKANVDYEK IVRDTCRAIG FVSDDVGLDA DNCKVLVNIE
110 120 130 140 150
QQSPDIAQGV HGHFTKRPEE IGAGDQGHMF GYATDETPEL MPLSHVLATK
160 170 180 190 200
LGARLTEVRK NGTCPWLRPD GKTQVTVEYR NDGGAMVPIR VHTVLISTQH
210 220 230 240 250
DETVTNDEIA ADLKEHVIKP VVPEQYLDEK TIFHLNPSGR FVIGGPHGDA
260 270 280 290 300
GLTGRKIIID TYGGWGAHGG GAFSGKDPTK VDRSGAYIVR QAAKSVVANG
310 320 330 340 350
LARRCIVQVS YAIGVPEPLS VFVDTYGTGK IPDKEILKIV KENFDFRPGM
360 370 380 390
ITINLDLKRG GNRFIKTAAY GHFGRDDPDF TWEVVKPLKW DKPAA
Length:395
Mass (Da):43,210
Last modified:December 1, 2001 - v1
Checksum:i240AA4A7CF8DA440
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF420238 mRNA Translation: AAL16064.1

Similar proteinsi

Entry informationi

Entry nameiMETK_DENCR
AccessioniPrimary (citable) accession number: Q944U4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: December 1, 2001
Last modified: March 15, 2017
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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