Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q944R1 (PEL15_ARATH)

Last modified October 13, 2009. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Probable pectate lyase 15
    EC=4.2.2.2
Alternative name(s):
    Pectate lyase A11
Gene names
Ordered Locus Names: At4g13710
ORF Names: F18A5.100
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Hide | Top

Protein attributes

Sequence length470 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

Hide | Top

General annotation (Comments)

Catalytic activity

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

Cofactor

Binds 1 calcium ion. Required for its activity By similarity.

Pathway

Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.

Tissue specificity

Expressed in flowers, but not in leaves. Ref.3

Sequence similarities

Belongs to the polysaccharide lyase 1 family.

Sequence caution

The sequence CAB36835.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAB78413.1 differs from that shown. Reason: Erroneous gene model prediction.

Hide | Top

Ontologies

Keywords
   DomainSignal
   LigandCalcium
Metal-binding
   Molecular functionLyase
   PTMGlycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

pectate lyase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 470448Probable pectate lyase 15
PRO_0000024880

Sites

Active site3481 Potential
Metal binding2681Calcium By similarity
Metal binding2921Calcium By similarity
Metal binding2961Calcium By similarity

Amino acid modifications

Glycosylation461N-linked (GlcNAc...) Potential
Glycosylation651N-linked (GlcNAc...) Potential
Glycosylation1061N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict135 – 1373HKN → AQE in AAB69762. Ref.3

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
Q944R1-1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 141373708998B95B

FASTA47051,936
        10         20         30         40         50         60 
MASSSQKLIS VCVAVLVVLA LTAMIFRNSE ISLSRKLKTE VIQSSNSSTM AAIRKLKTEE 

        70         80         90        100        110        120 
FQSLNSSTMA ATRLDGEPQQ QQHAVADDPD MVADEVAKLV QMSEQNRTAR RKLGFFSCGT 

       130        140        150        160        170        180 
GNPIDDCWRC DRNWHKNRKR LADCGIGFGR NAIGGRDGRF YIVTDPTDED VVNPKPGTLR 

       190        200        210        220        230        240 
HAVIQEEPLW IVFKRDMVIE LKQELIMNSF KTIDARGSNV HIANGACITI QFITNVIIHG 

       250        260        270        280        290        300 
LHIHDCKPTG NAMVRSSPSH FGWRTMADGD AVSIFGSSHI WIDHNSLSHC ADGLVDAVMG 

       310        320        330        340        350        360 
STAITVSNNH FTHHNEVMLL GHSDSYTKDK LMQVTIAYNH FGEGLVQRMP RCRHGYFHVV 

       370        380        390        400        410        420 
NNDYTHWEMY AIGGSAEPTI NSQGNRYAAP MDRFAKEVTK RVETDASEWK KWNWRSEGDL 

       430        440        450        460        470 
LLNGAFFRPS GAGASASYGR ASSLAAKPSS MVDTITSTAG ALGCRKGRPC

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed: 10617198] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[3]"Identification of the tobacco and Arabidopsis homologues of the pollen-expressed LAT59 gene of tomato."
Kulikauskas R., McCormick S.
Plant Mol. Biol. 34:809-814(1997) [PubMed: 9278171] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 135-361, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases

AL035528 Genomic DNA. Translation: CAB36835.1. Sequence problems.
AL161537 Genomic DNA. Translation: CAB78413.1. Sequence problems.
AF424592 mRNA. Translation: AAL11586.1.
AY142013 mRNA. Translation: AAM98277.1.
U83622 Genomic DNA. Translation: AAB69762.1.
IPIIPI00540091.
PIRH85148.
T05240.
RefSeqNP_567409.1.
UniGeneAt.22271

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ944R1.

Protein family/group databases

CAZyPL1. Polysaccharide Lyase Family 1.

Genome annotation databases

GeneID827005.
GenomeReviewsGene locus AT4G13710 in contig CT486007_GR.
KEGGath:AT4G13710.
NMPDRfig|3702.1.peg.19063.

Organism-specific databases

TAIRAt4g13710.

Enzyme and pathway databases

BRENDA4.2.2.2. 302.

Gene expression databases

ArrayExpressQ944R1.
GenevestigatorQ944R1.
GermOnlineAT4G13710. Arabidopsis thaliana.

Family and domain databases

InterProIPR002022. Amb_allergen.
IPR018082. AmbAllergen.
IPR012334. Pectin_lyas_fold.
[Graphical view]
Gene3DG3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
PfamPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
PRINTSPR00807. AMBALLERGEN.
SMARTSM00656. Amb_all. 1 hit.
[Graphical view]
ProtoNetSearch...

Hide | Top

Entry information

Entry namePEL15_ARATH
AccessionPrimary (citable) accession number: Q944R1
Secondary accession number(s): O23668, Q9SVP1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: December 1, 2001
Last modified: October 13, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Hide | Top

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents