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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit

Gene

OST48

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.1 Publication

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • plant-type cell wall organization Source: TAIR
  • protein N-linked glycosylation via asparagine Source: TAIR
  • unidimensional cell growth Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT5G66680-MONOMER.
ReactomeiR-ATH-6798695. Neutrophil degranulation.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit (EC:2.4.99.18)
Short name:
Oligosaccharyl transferase 48 kDa subunit
Alternative name(s):
Protein DEFECTIVE GLYCOSYLATION 1
Gene namesi
Name:OST48
Synonyms:DGL1
Ordered Locus Names:At5g66680
ORF Names:MSN2.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G66680.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 414LumenalSequence analysisAdd BLAST390
Transmembranei415 – 435HelicalSequence analysisAdd BLAST21
Topological domaini436 – 437CytoplasmicSequence analysis2

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • Golgi apparatus Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: TAIR
  • nucleolus Source: TAIR
  • oligosaccharyltransferase complex Source: TAIR
  • plant-type cell wall Source: TAIR
  • plasmodesma Source: TAIR
  • vacuolar membrane Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Embryo-lethal.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000042081325 – 437Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunitAdd BLAST413

Proteomic databases

PaxDbiQ944K2.
PRIDEiQ944K2.

PTM databases

iPTMnetiQ944K2.
SwissPalmiQ944K2.

Expressioni

Gene expression databases

GenevisibleiQ944K2. AT.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex.By similarity

Protein-protein interaction databases

BioGridi22043. 21 interactors.
STRINGi3702.AT5G66680.1.

Structurei

3D structure databases

ProteinModelPortaliQ944K2.
SMRiQ944K2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DDOST 48 kDa subunit family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2754. Eukaryota.
ENOG410XSF3. LUCA.
HOGENOMiHOG000173744.
InParanoidiQ944K2.
KOiK12670.
OMAiAFSMMIG.
OrthoDBiEOG09360E0E.
PhylomeDBiQ944K2.

Family and domain databases

InterProiIPR005013. DDOST_48_kDa_subunit.
[Graphical view]
PANTHERiPTHR10830. PTHR10830. 1 hit.
PfamiPF03345. DDOST_48kD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q944K2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNLSRSVAL ISVFLLPLLS FSFSVDNPTD RRVLVLLDDL SLKSSHSIFF
60 70 80 90 100
NTLKSRGFDL DFKLAEDSKL ALQRYGQYLY DGLIIFAPST ERFGGSLDSK
110 120 130 140 150
SIADFVDSGR DLILSADTAA SDLIRGIATE CGVDFDEDSS AMVIDHTSFS
160 170 180 190 200
VSDVDGDHTL IAADDLVKSD VILGKTKIEA PVLFRGVAHS LNPTNNLVLK
210 220 230 240 250
VLSASPSAYS ANPSSKLSSP PQLTGSSISL VSVMQARNNA RVVISGSVQL
260 270 280 290 300
FSDRLIRSGV QKAGSPNQYE KSGNEQFVTE LSKWVFHERG HLKAGNLVHH
310 320 330 340 350
RVGETDEPAI YRIKDDLEFS VEIYEWSGKS WEPYVANDVQ VQFYMMSPYV
360 370 380 390 400
LKTLSTDKKG LFHTSFKVPD VYGVFQFKVE YEKLGYTTLS LSKQIPVRPY
410 420 430
RHNEYERFIP TAYPYYGACF TTMAGFFVFS FVYLYHK
Length:437
Mass (Da):48,744
Last modified:December 1, 2001 - v1
Checksum:iA227381107CD0287
GO

Sequence cautioni

The sequence BAA97274 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti265S → I in AAM63023 (Ref. 4) Curated1
Sequence conflicti338D → G in AAK59450 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018119 Genomic DNA. Translation: BAA97274.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED98251.1.
AF428338 mRNA. Translation: AAL16268.1.
AY034944 mRNA. Translation: AAK59450.1.
AY150381 mRNA. Translation: AAN12926.1.
AY085807 mRNA. Translation: AAM63023.1.
RefSeqiNP_569038.1. NM_126066.4.
UniGeneiAt.23530.

Genome annotation databases

EnsemblPlantsiAT5G66680.1; AT5G66680.1; AT5G66680.
GeneIDi836801.
GrameneiAT5G66680.1; AT5G66680.1; AT5G66680.
KEGGiath:AT5G66680.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018119 Genomic DNA. Translation: BAA97274.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED98251.1.
AF428338 mRNA. Translation: AAL16268.1.
AY034944 mRNA. Translation: AAK59450.1.
AY150381 mRNA. Translation: AAN12926.1.
AY085807 mRNA. Translation: AAM63023.1.
RefSeqiNP_569038.1. NM_126066.4.
UniGeneiAt.23530.

3D structure databases

ProteinModelPortaliQ944K2.
SMRiQ944K2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22043. 21 interactors.
STRINGi3702.AT5G66680.1.

PTM databases

iPTMnetiQ944K2.
SwissPalmiQ944K2.

Proteomic databases

PaxDbiQ944K2.
PRIDEiQ944K2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G66680.1; AT5G66680.1; AT5G66680.
GeneIDi836801.
GrameneiAT5G66680.1; AT5G66680.1; AT5G66680.
KEGGiath:AT5G66680.

Organism-specific databases

TAIRiAT5G66680.

Phylogenomic databases

eggNOGiKOG2754. Eukaryota.
ENOG410XSF3. LUCA.
HOGENOMiHOG000173744.
InParanoidiQ944K2.
KOiK12670.
OMAiAFSMMIG.
OrthoDBiEOG09360E0E.
PhylomeDBiQ944K2.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciARA:AT5G66680-MONOMER.
ReactomeiR-ATH-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ944K2.

Gene expression databases

GenevisibleiQ944K2. AT.

Family and domain databases

InterProiIPR005013. DDOST_48_kDa_subunit.
[Graphical view]
PANTHERiPTHR10830. PTHR10830. 1 hit.
PfamiPF03345. DDOST_48kD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOST48_ARATH
AccessioniPrimary (citable) accession number: Q944K2
Secondary accession number(s): Q8LDT6, Q94CD6, Q9LVR2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.