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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit

Gene

OST48

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.1 Publication

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • plant-type cell wall organization Source: TAIR
  • protein N-linked glycosylation via asparagine Source: TAIR
  • unidimensional cell growth Source: TAIR

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-ATH-6798695 Neutrophil degranulation
UniPathwayiUPA00378

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit (EC:2.4.99.18)
Short name:
Oligosaccharyl transferase 48 kDa subunit
Alternative name(s):
Protein DEFECTIVE GLYCOSYLATION 1
Gene namesi
Name:OST48
Synonyms:DGL1
Ordered Locus Names:At5g66680
ORF Names:MSN2.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G66680
TAIRilocus:2173659 AT5G66680

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 414LumenalSequence analysisAdd BLAST390
Transmembranei415 – 435HelicalSequence analysisAdd BLAST21
Topological domaini436 – 437CytoplasmicSequence analysis2

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Embryo-lethal.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000042081325 – 437Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunitAdd BLAST413

Proteomic databases

PaxDbiQ944K2
PRIDEiQ944K2

PTM databases

iPTMnetiQ944K2
SwissPalmiQ944K2

Expressioni

Gene expression databases

ExpressionAtlasiQ944K2 baseline and differential
GenevisibleiQ944K2 AT

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex.By similarity

Protein-protein interaction databases

BioGridi22043, 21 interactors
STRINGi3702.AT5G66680.1

Structurei

3D structure databases

SMRiQ944K2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DDOST 48 kDa subunit family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2754 Eukaryota
ENOG410XSF3 LUCA
HOGENOMiHOG000173744
InParanoidiQ944K2
KOiK12670
OMAiAFSMMIG
OrthoDBiEOG09360E0E
PhylomeDBiQ944K2

Family and domain databases

InterProiView protein in InterPro
IPR005013 DDOST_48_kDa_subunit
PANTHERiPTHR10830 PTHR10830, 1 hit
PfamiView protein in Pfam
PF03345 DDOST_48kD, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q944K2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNLSRSVAL ISVFLLPLLS FSFSVDNPTD RRVLVLLDDL SLKSSHSIFF
60 70 80 90 100
NTLKSRGFDL DFKLAEDSKL ALQRYGQYLY DGLIIFAPST ERFGGSLDSK
110 120 130 140 150
SIADFVDSGR DLILSADTAA SDLIRGIATE CGVDFDEDSS AMVIDHTSFS
160 170 180 190 200
VSDVDGDHTL IAADDLVKSD VILGKTKIEA PVLFRGVAHS LNPTNNLVLK
210 220 230 240 250
VLSASPSAYS ANPSSKLSSP PQLTGSSISL VSVMQARNNA RVVISGSVQL
260 270 280 290 300
FSDRLIRSGV QKAGSPNQYE KSGNEQFVTE LSKWVFHERG HLKAGNLVHH
310 320 330 340 350
RVGETDEPAI YRIKDDLEFS VEIYEWSGKS WEPYVANDVQ VQFYMMSPYV
360 370 380 390 400
LKTLSTDKKG LFHTSFKVPD VYGVFQFKVE YEKLGYTTLS LSKQIPVRPY
410 420 430
RHNEYERFIP TAYPYYGACF TTMAGFFVFS FVYLYHK
Length:437
Mass (Da):48,744
Last modified:December 1, 2001 - v1
Checksum:iA227381107CD0287
GO

Sequence cautioni

The sequence BAA97274 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti265S → I in AAM63023 (Ref. 4) Curated1
Sequence conflicti338D → G in AAK59450 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018119 Genomic DNA Translation: BAA97274.1 Sequence problems.
CP002688 Genomic DNA Translation: AED98251.1
AF428338 mRNA Translation: AAL16268.1
AY034944 mRNA Translation: AAK59450.1
AY150381 mRNA Translation: AAN12926.1
AY085807 mRNA Translation: AAM63023.1
RefSeqiNP_569038.1, NM_126066.4
UniGeneiAt.23530

Genome annotation databases

EnsemblPlantsiAT5G66680.1; AT5G66680.1; AT5G66680
GeneIDi836801
GrameneiAT5G66680.1; AT5G66680.1; AT5G66680
KEGGiath:AT5G66680

Similar proteinsi

Entry informationi

Entry nameiOST48_ARATH
AccessioniPrimary (citable) accession number: Q944K2
Secondary accession number(s): Q8LDT6, Q94CD6, Q9LVR2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: December 1, 2001
Last modified: April 25, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health