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Protein

Fructose-bisphosphate aldolase 2, chloroplastic

Gene

FBA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a key role in glycolysis and gluconeogenesis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.By similarity

Kineticsi

  1. KM=35 µM for fructose-bisphosphate for the cleavage reaction1 Publication
  1. Vmax=13 µmol/min/mg enzyme with fructose-bisphosphate as substrate1 Publication

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.Curated
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (PGI1 (At4g24620)), Glucose-6-phosphate isomerase 1, chloroplastic (PGI1), Glucose-6-phosphate isomerase (PGIC), Glucose-6-phosphate isomerase, cytosolic (PGIC)
  3. ATP-dependent 6-phosphofructokinase 1 (PFK1), ATP-dependent 6-phosphofructokinase 5, chloroplastic (PFK5), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 1 (PFP-ALPHA1), ATP-dependent 6-phosphofructokinase 6 (PFK6), ATP-dependent 6-phosphofructokinase 3 (PFK3), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 2 (PFP-ALPHA2), ATP-dependent 6-phosphofructokinase 4, chloroplastic (PFK4), ATP-dependent 6-phosphofructokinase 2 (PFK2), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 1 (PFP-BETA1), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 2 (PFP-BETA2), ATP-dependent 6-phosphofructokinase 7 (PFK7)
  4. Fructose-bisphosphate aldolase (M3E9.40), Fructose-bisphosphate aldolase (At2g36460), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (FBA2), Fructose-bisphosphate aldolase 1, chloroplastic (FBA1), Fructose-bisphosphate aldolase (F8J2_100), Fructose-bisphosphate aldolase 7, cytosolic (FBA7), Fructose-bisphosphate aldolase 3, chloroplastic (FBA3), Fructose-bisphosphate aldolase (At2g36460), Fructose-bisphosphate aldolase 2, chloroplastic (FBA2), Fructose-bisphosphate aldolase (At2g21330), Fructose-bisphosphate aldolase 4, cytosolic (FBA4)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei95 – 951SubstrateBy similarity
Binding sitei185 – 1851SubstrateBy similarity
Active sitei225 – 2251Proton acceptorBy similarity
Active sitei267 – 2671Schiff-base intermediate with dihydroxyacetone-PBy similarity
Sitei398 – 3981Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphateBy similarity

GO - Molecular functioni

GO - Biological processi

  • glycolytic process Source: UniProtKB-UniPathway
  • response to abscisic acid Source: TAIR
  • response to cadmium ion Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

BioCyciARA:AT4G38970-MONOMER.
ARA:GQT-2393-MONOMER.
UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase 2, chloroplasticCurated (EC:4.1.2.13Curated)
Short name:
AtFBA21 Publication
Gene namesi
Name:FBA21 Publication
Ordered Locus Names:At4g38970
ORF Names:F19H22.70
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G38970.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast stroma Source: TAIR
  • membrane Source: TAIR
  • plastoglobule Source: TAIR
  • thylakoid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Reduced growth rate. Unability to accumulate starch in leaves during daylight.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi394 – 3941K → A: Loss of methylation, but no effect on enzyme activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4646ChloroplastSequence analysisAdd
BLAST
Chaini47 – 398352Fructose-bisphosphate aldolase 2, chloroplasticPRO_0000286527Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei157 – 1571PhosphoserineCombined sources
Modified residuei215 – 2151PhosphoserineCombined sources
Modified residuei394 – 3941N6,N6,N6-trimethyllysine1 Publication

Post-translational modificationi

Can be trimethylated at Lys-394 by LSMT-L. The methylation level has no influence on the ologomerization state or on the kinetic properties of the enzyme.1 Publication
Phosphorylated on tyrosine residues in response to abscisic acid (ABA) in germinating seeds.1 Publication

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ944G9.
PRIDEiQ944G9.

PTM databases

iPTMnetiQ944G9.

Expressioni

Tissue specificityi

Highly expressed in rosettes leaves.1 Publication

Inductioni

By glucose and sucrose (PubMed:22561114). Induced by drought stress (PubMed:22561114).1 Publication

Gene expression databases

ExpressionAtlasiQ944G9. baseline and differential.
GenevisibleiQ944G9. AT.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

BioGridi15332. 3 interactions.
STRINGi3702.AT4G38970.1.

Structurei

3D structure databases

ProteinModelPortaliQ944G9.
SMRiQ944G9. Positions 52-380.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni309 – 3113Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1557. Eukaryota.
COG3588. LUCA.
HOGENOMiHOG000220876.
InParanoidiQ944G9.
KOiK01623.
OMAiVRSHPSG.
OrthoDBiEOG09360CPA.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR029768. Aldolase_I_AS.
IPR013785. Aldolase_TIM.
IPR000741. FBA_I.
[Graphical view]
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q944G9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASTSLLKAS PVLDKSEWVK GQSVLFRQPS SASVVLRNRA TSLTVRAASS
60 70 80 90 100
YADELVKTAK TIASPGRGIL AMDESNATCG KRLDSIGLEN TEANRQAFRT
110 120 130 140 150
LLVSAPGLGQ YVSGAILFEE TLYQSTTEGK KMVDVLVEQN IVPGIKVDKG
160 170 180 190 200
LVPLVGSNNE SWCQGLDGLS SRTAAYYQQG ARFAKWRTVV SIPNGPSALA
210 220 230 240 250
VKEAAWGLAR YAAISQDSGL VPIVEPEILL DGEHDIDRTY DVAEKVWAEV
260 270 280 290 300
FFYLAQNNVM FEGILLKPSM VTPGAESKDR ATPEQVAAYT LKLLRNRVPP
310 320 330 340 350
AVPGIMFLSG GQSEVEATLN LNAMNQAPNP WHVSFSYARA LQNTCLKTWG
360 370 380 390
GRPENVNAAQ TTLLARAKAN SLAQLGKYTG EGESEEAKEG MFVKGYTY
Length:398
Mass (Da):42,988
Last modified:July 27, 2011 - v2
Checksum:iE306F39ED18BD71D
GO

Sequence cautioni

The sequence CAB38817 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAB80560 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti163 – 1631C → G in AAL16224 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL035679 Genomic DNA. Translation: CAB38817.1. Sequence problems.
AL161594 Genomic DNA. Translation: CAB80560.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE87002.1.
AF428455 mRNA. Translation: AAL16224.1.
BT015870 mRNA. Translation: AAU94433.1.
AK226247 mRNA. Translation: BAE98409.1.
PIRiT06057.
RefSeqiNP_568049.1. NM_120057.3. [Q944G9-1]
UniGeneiAt.21716.
At.24925.
At.72607.
At.75329.

Genome annotation databases

EnsemblPlantsiAT4G38970.1; AT4G38970.1; AT4G38970. [Q944G9-1]
GeneIDi830052.
KEGGiath:AT4G38970.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL035679 Genomic DNA. Translation: CAB38817.1. Sequence problems.
AL161594 Genomic DNA. Translation: CAB80560.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE87002.1.
AF428455 mRNA. Translation: AAL16224.1.
BT015870 mRNA. Translation: AAU94433.1.
AK226247 mRNA. Translation: BAE98409.1.
PIRiT06057.
RefSeqiNP_568049.1. NM_120057.3. [Q944G9-1]
UniGeneiAt.21716.
At.24925.
At.72607.
At.75329.

3D structure databases

ProteinModelPortaliQ944G9.
SMRiQ944G9. Positions 52-380.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi15332. 3 interactions.
STRINGi3702.AT4G38970.1.

PTM databases

iPTMnetiQ944G9.

Proteomic databases

PaxDbiQ944G9.
PRIDEiQ944G9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G38970.1; AT4G38970.1; AT4G38970. [Q944G9-1]
GeneIDi830052.
KEGGiath:AT4G38970.

Organism-specific databases

TAIRiAT4G38970.

Phylogenomic databases

eggNOGiKOG1557. Eukaryota.
COG3588. LUCA.
HOGENOMiHOG000220876.
InParanoidiQ944G9.
KOiK01623.
OMAiVRSHPSG.
OrthoDBiEOG09360CPA.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.
BioCyciARA:AT4G38970-MONOMER.
ARA:GQT-2393-MONOMER.

Miscellaneous databases

PROiQ944G9.

Gene expression databases

ExpressionAtlasiQ944G9. baseline and differential.
GenevisibleiQ944G9. AT.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR029768. Aldolase_I_AS.
IPR013785. Aldolase_TIM.
IPR000741. FBA_I.
[Graphical view]
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALFP2_ARATH
AccessioniPrimary (citable) accession number: Q944G9
Secondary accession number(s): Q5XEU6, Q9SVJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.