Reviewed, UniProtKB/Swiss-Prot Q944G9 (ALFC2_ARATH)

Names and origin

Protein names

Recommended name:
    Probable fructose-bisphosphate aldolase 2, chloroplastic
    EC=4.1.2.13

Gene names

Name:	FBA2
Ordered Locus Names:	At4g38970
ORF Names:	F19H22.70

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Subcellular location	Plastid › chloroplast › plastoglobule. Ref.5 Ref.6
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.
Sequence caution	The sequence CAB38817.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB80560.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW response to abscisic acid stimulus Inferred from expression pattern. Source: TAIR response to cadmium ion Inferred from expression pattern. Source: TAIR
Cellular component	apoplast Inferred from direct assay. Source: TAIR chloroplast envelope Inferred from direct assay. Source: TAIR chloroplast stroma Ref.5 Inferred from direct assay. Source: TAIR membrane Inferred from direct assay. Source: TAIR plastoglobule Ref.5 Ref.6 Inferred from direct assay. Source: TAIR thylakoid Inferred from direct assay. Source: TAIR
Molecular function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q944G9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 46	46	Chloroplast Potential
	Chain	47 – 398	352	Probable fructose-bisphosphate aldolase 2, chloroplastic		PRO_0000286527
Sites
	Active site	225	1	Proton acceptor By similarity
	Active site	267	1	Schiff-base intermediate with dihydroxyacetone-P By similarity
	Binding site	95	1	Substrate By similarity
	Binding site	185	1	Substrate By similarity
	Site	398	1	Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate By similarity
Experimental info
	Sequence conflict	163	1	G → C in AAU94433. Ref.3
	Sequence conflict	163	1	G → C in BAE98409. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 4EB028F22704BFE9	FASTA	398	42,942
	10 20 30 40 50 60 MASTSLLKAS PVLDKSEWVK GQSVLFRQPS SASVVLRNRA TSLTVRAASS YADELVKTAK 70 80 90 100 110 120 TIASPGRGIL AMDESNATCG KRLDSIGLEN TEANRQAFRT LLVSAPGLGQ YVSGAILFEE 130 140 150 160 170 180 TLYQSTTEGK KMVDVLVEQN IVPGIKVDKG LVPLVGSNNE SWGQGLDGLS SRTAAYYQQG 190 200 210 220 230 240 ARFAKWRTVV SIPNGPSALA VKEAAWGLAR YAAISQDSGL VPIVEPEILL DGEHDIDRTY 250 260 270 280 290 300 DVAEKVWAEV FFYLAQNNVM FEGILLKPSM VTPGAESKDR ATPEQVAAYT LKLLRNRVPP 310 320 330 340 350 360 AVPGIMFLSG GQSEVEATLN LNAMNQAPNP WHVSFSYARA LQNTCLKTWG GRPENVNAAQ 370 380 390 TTLLARAKAN SLAQLGKYTG EGESEEAKEG MFVKGYTY

References

[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Arabidopsis ORF clones." Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Tocopherol cyclase (VTE1) localization and vitamin E accumulation in chloroplast plastoglobule lipoprotein particles." Vidi P.-A., Kanwischer M., Baginsky S., Austin J.R., Csucs G., Doermann P., Kessler F., Brehelin C. J. Biol. Chem. 281:11225-11234(2006) [PubMed: 16414959] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[6]	"Protein profiling of plastoglobules in chloroplasts and chromoplasts. A surprising site for differential accumulation of metabolic enzymes." Ytterberg A.J., Peltier J.-B., van Wijk K.J. Plant Physiol. 140:984-997(2006) [PubMed: 16461379] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL035679 Genomic DNA. Translation: CAB38817.1. Sequence problems. AL161594 Genomic DNA. Translation: CAB80560.1. Sequence problems. AF428455 mRNA. Translation: AAL16224.1. BT015870 mRNA. Translation: AAU94433.1. AK226247 mRNA. Translation: BAE98409.1.
IPI	IPI00541448.
PIR	T06057.
RefSeq	NP_568049.1.
UniGene	At.21716 At.24925 At.72607
3D structure databases
HSSP	HSSP built from PDB template 1J4E based on UniProtKB P00883.
ModBase	Search...
Protein-protein interaction databases
STRING	Q944G9.
Proteomic databases
PRIDE	Q944G9.
ProMEX	Q944G9.
Genome annotation databases
GeneID	830052.
GenomeReviews	Gene locus AT4G38970 in contig CT486007_GR.
Organism-specific databases
TAIR	At4g38970.
Enzyme and pathway databases
BRENDA	4.1.2.13. 302.
Gene expression databases
Genevestigator	Q944G9.
Family and domain databases
InterPro	IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11627. Aldolase_I. 1 hit.
Pfam	PF00274. Glycolytic. 1 hit. [Graphical view]
ProDom	PD001128. Aldolase_I. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00158. ALDOLASE_CLASS_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALFC2_ARATH

Accession

Primary (citable) accession number: Q944G9
Secondary accession number(s): Q5XEU6, Q9SVJ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	December 1, 2001
Last modified:	October 13, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)