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Protein

Protein NRT1/ PTR FAMILY 2.10

Gene

NPF2.10

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

High-affinity, proton-dependent glucosinolate-specific transporter. Involved in the distribution of glucosinolates within the leaf, including import into the glucosinolate-rich S-cells located adjacent to the phloem. Involved in bidirectional long-distance transport of aliphatic but not indole glucosinolates. May be involved in removal of glucosinolates from the xylem in roots.2 Publications

Kineticsi

  1. KM=20 µM for 4-methylthiobutyl glucosinolate1 Publication

    GO - Molecular functioni

    • glucosinolate:proton symporter activity Source: TAIR

    GO - Biological processi

    • glucosinolate transport Source: TAIR
    • phloem glucosinolate loading Source: TAIR

    Keywordsi

    Biological processTransport

    Enzyme and pathway databases

    ReactomeiR-ATH-427975 Proton/oligopeptide cotransporters
    R-ATH-6798695 Neutrophil degranulation

    Protein family/group databases

    TCDBi2.A.17.3.17 the proton-dependent oligopeptide transporter (pot/ptr) family

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein NRT1/ PTR FAMILY 2.10
    Short name:
    AtNPF2.10
    Alternative name(s):
    Protein GLUCOSINOLATE TRANSPORTER-1
    Gene namesi
    Name:NPF2.10
    Synonyms:GTR1
    Ordered Locus Names:At3g47960
    ORF Names:T17F15.170
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 3

    Organism-specific databases

    AraportiAT3G47960
    TAIRilocus:2097910 AT3G47960

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei77 – 97HelicalSequence analysisAdd BLAST21
    Transmembranei105 – 125HelicalSequence analysisAdd BLAST21
    Transmembranei136 – 156HelicalSequence analysisAdd BLAST21
    Transmembranei175 – 195HelicalSequence analysisAdd BLAST21
    Transmembranei221 – 241HelicalSequence analysisAdd BLAST21
    Transmembranei249 – 269HelicalSequence analysisAdd BLAST21
    Transmembranei367 – 387HelicalSequence analysisAdd BLAST21
    Transmembranei410 – 430HelicalSequence analysisAdd BLAST21
    Transmembranei451 – 471HelicalSequence analysisAdd BLAST21
    Transmembranei499 – 519HelicalSequence analysisAdd BLAST21
    Transmembranei535 – 555HelicalSequence analysisAdd BLAST21
    Transmembranei582 – 602HelicalSequence analysisAdd BLAST21

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    No effect on the total glucosinolate levels in seeds. Gtr1 and gtr2 double mutant has no detectable glucosinolate in seeds.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003999781 – 636Protein NRT1/ PTR FAMILY 2.10Add BLAST636

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei52PhosphoserineCombined sources1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ944G5
    PRIDEiQ944G5

    PTM databases

    iPTMnetiQ944G5

    Expressioni

    Tissue specificityi

    Expressed in stems, flowers, siliques, roots, shoots and leaves. Expressed in veins and in adjacent mesophyll cells in leaves, and in the root vasculature with highest expression in lateral branching points.3 Publications

    Inductioni

    Up-regulated by wounding.1 Publication

    Gene expression databases

    ExpressionAtlasiQ944G5 baseline and differential
    GenevisibleiQ944G5 AT

    Interactioni

    Protein-protein interaction databases

    BioGridi9271, 9 interactors
    STRINGi3702.AT3G47960.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ944G5
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi42 – 47Poly-Thr6

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1237 Eukaryota
    COG3104 LUCA
    HOGENOMiHOG000237399
    InParanoidiQ944G5
    OMAiHQPGTAN
    OrthoDBiEOG093606CO

    Family and domain databases

    CDDicd06174 MFS, 1 hit
    InterProiView protein in InterPro
    IPR020846 MFS_dom
    IPR036259 MFS_trans_sf
    IPR000109 POT_fam
    PANTHERiPTHR11654 PTHR11654, 1 hit
    PfamiView protein in Pfam
    PF00854 PTR2, 1 hit
    SUPFAMiSSF103473 SSF103473, 2 hits

    Sequencei

    Sequence statusi: Complete.

    Q944G5-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKSRVILNHR DRRDKNHNNN NTNHYTQVDT MERKPLEVEP STTTTNTDVV
    60 70 80 90 100
    DSFEEEQRKI VYRGWKVMPF IIGNETFEKL GIIGTLSNLL VYLTSVFNLK
    110 120 130 140 150
    SYTAATIINA FSGTINFGTF IAAFLCDTYF GRYKTLSVAV IACFLGSFVI
    160 170 180 190 200
    LLTAAIPSLH PVACGNKISC EGPSVGQILF LLMGLGFLVV GAGGIRPCNL
    210 220 230 240 250
    AFGADQFNPK SESGKKGINS FFNWYFFTFT FAQIISLTAV VYIQSNVSWT
    260 270 280 290 300
    IGLIIPVALM FLACVIFFAG DRLYVKVKAS GSPLAGIARV IAAAIKKRGL
    310 320 330 340 350
    KPVKQPWVNL YNHIPSNYAN TTLKYTDQFR FLDKAAIMTP EEKLNSDGTA
    360 370 380 390 400
    SDPWKLCTLQ QVEEVKCIVR VIPIWFASTI YYLAITIQMT YPVFQALQSD
    410 420 430 440 450
    RRLGSGGFRI PAATYVVFLM TGMTVFIIFY DRVLVPSLRR VTGLETGISL
    460 470 480 490 500
    LQRIGAGFTF AIMSLLVSGF IEERRRNFAL TKPTLGMAPR TGEISSMSAL
    510 520 530 540 550
    WLIPQLTLAG IAEAFAAIGQ MEFYYKQFPE NMKSFAGSIF YVGAGVSSYL
    560 570 580 590 600
    ASFLISTVHR TTAHSPSGNW LAEDLNKAKL DYFYFMLTGL MVVNMAYFLL
    610 620 630
    MARWYRYKGG NDEDITEIET NEEETKQQQL QDKNSV
    Length:636
    Mass (Da):71,040
    Last modified:July 27, 2011 - v3
    Checksum:i6C5E5410ED6CFBF1
    GO

    Sequence cautioni

    The sequence AAK44017 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
    The sequence AAL16236 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti92Y → C in AAL16236 (PubMed:14593172).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL049658 Genomic DNA Translation: CAB41143.1
    CP002686 Genomic DNA Translation: AEE78351.1
    AF370202 mRNA Translation: AAK44017.1 Different initiation.
    AF428467 mRNA Translation: AAL16236.1 Different initiation.
    PIRiT06687
    RefSeqiNP_566896.2, NM_114665.4
    UniGeneiAt.20263
    At.71210

    Genome annotation databases

    EnsemblPlantsiAT3G47960.1; AT3G47960.1; AT3G47960
    GeneIDi823951
    GrameneiAT3G47960.1; AT3G47960.1; AT3G47960
    KEGGiath:AT3G47960

    Similar proteinsi

    Entry informationi

    Entry nameiPTR44_ARATH
    AccessioniPrimary (citable) accession number: Q944G5
    Secondary accession number(s): Q94K82, Q9SU59
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2010
    Last sequence update: July 27, 2011
    Last modified: April 25, 2018
    This is version 72 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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