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Q944C2 (PLCD5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoinositide phospholipase C 5

EC=3.1.4.11
Alternative name(s):
Phosphoinositide phospholipase PLC5
Short name=AtPLC5
Short name=AtPLCx
Short name=PI-PLC5
Gene names
Name:PLC5
Synonyms:ATHATPLC6
Ordered Locus Names:At5g58690
ORF Names:MZN1.13, MZN1.15
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length578 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Ref.6

Catalytic activity

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactor

Calcium.

Subcellular location

Cytoplasmcytosol. Cell membrane; Peripheral membrane protein.

Tissue specificity

Expressed in leaves, roots, flowers and siliques. High levels of expression in the guard cells and vasculature of leaves and roots. Ref.6

Induction

By environmental stresses such as dehydration, salinity and low temperature. Ref.6

Sequence similarities

Contains 1 C2 domain.

Contains 1 EF-hand-like domain.

Contains 1 PI-PLC X-box domain.

Contains 1 PI-PLC Y-box domain.

Sequence caution

The sequence BAA97337.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 578578Phosphoinositide phospholipase C 5
PRO_0000324130

Regions

Domain28 – 11184EF-hand-like
Domain112 – 256145PI-PLC X-box
Domain314 – 430117PI-PLC Y-box
Domain436 – 543108C2

Sites

Active site1271 By similarity
Active site1731 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q944C2 [UniParc].

Last modified June 1, 2002. Version 2.
Checksum: A744F73545FC7364

FASTA57866,253
        10         20         30         40         50         60 
MKRDMGSYKM GLCCSDKLRM NRGAPPQDVV TAFVEYTEGR SHMTAEQLCR FLVEVQDETE 

        70         80         90        100        110        120 
VLVSDAEKII ERITCERHHI TKFLRHTLNL DDFFSFLFSD DLNHPIDSKV HQDMASPLSH 

       130        140        150        160        170        180 
YFIYTSHNSY LTGNQINSEC SDVPLIKALK RGVRALELDM WPNSTKDDIL VLHGWAWTPP 

       190        200        210        220        230        240 
VELVKCLRSI KEHAFYASAY PVILTLEDHL TPDLQAKAAE MMKEIFMDMV YFPEAGGLKE 

       250        260        270        280        290        300 
FPSPEDLKYK IVISTKPPKG SLRKDKDSES DASGKASSDV SADDEKTEEE TSEAKNEEDG 

       310        320        330        340        350        360 
FDQESSNLDF LTYSRLITIP SGNAKNGLKE ALTIDNGGVR RLSLREQKFK KATEMYGTEV 

       370        380        390        400        410        420 
IKFTQKNLLR IYPKATRVNS SNYRPYNGWM YGAQMVAFNM QGYGRALWMM HGMFRGNGGC 

       430        440        450        460        470        480 
GYVKKPDFMM NNNLSGEVFN PKAKLPIKKT LKVKVYMGKG WDSGFQRTCF NTWSSPNFYT 

       490        500        510        520        530        540 
RVGITGVRGD KVMKKTKKEQ KTWEPFWNEE FEFQLTVPEL ALLRIEVHDY NMPEKDDFSG 

       550        560        570 
QTCLPVSELR QGIRSVPLYD RKGERLVSVT LLMRFHFL 

« Hide

References

« Hide 'large scale' references
[1]"Preparation of polyclonal antibody specific for AtPLC4, an Arabidopsis phosphatidylinositol-specific phospholipase C in rabbits."
Cao Z., Zhang J., Li Y., Xu X., Liu G., Bhattacharrya M.K., Yang H., Ren D.
Protein Expr. Purif. 52:306-312(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
Mueller-Roeber B., Pical C.
Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[6]"Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms."
Hunt L., Otterhag L., Lee J.C., Lasheen T., Hunt J., Seki M., Shinozaki K., Sommarin M., Gilmour D.J., Pical C., Gray J.E.
New Phytol. 162:643-654(2004) [AGRICOLA] [Europe PMC]
Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF434167 mRNA. Translation: AAL30748.2.
AB020755 Genomic DNA. Translation: BAA97337.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED97085.1.
AY062681 mRNA. Translation: AAL32759.1.
BT010399 mRNA. Translation: AAQ56842.1.
RefSeqNP_200677.2. NM_125256.2.
UniGeneAt.27264.

3D structure databases

ProteinModelPortalQ944C2.
SMRQ944C2. Positions 32-574.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT5G58690.1-P.

Proteomic databases

PRIDEQ944C2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G58690.1; AT5G58690.1; AT5G58690.
GeneID835983.
KEGGath:AT5G58690.

Organism-specific databases

TAIRAT5G58690.

Phylogenomic databases

eggNOGNOG149692.
HOGENOMHOG000244119.
InParanoidQ944C2.
KOK05857.
OMAMPEKDDF.
PhylomeDBQ944C2.

Enzyme and pathway databases

BioCycARA:AT5G58690-MONOMER.

Gene expression databases

GenevestigatorQ944C2.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERPTHR10336. PTHR10336. 1 hit.
PfamPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSPR00390. PHPHLIPASEC.
SMARTSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePLCD5_ARATH
AccessionPrimary (citable) accession number: Q944C2
Secondary accession number(s): Q8W4B4, Q9LUZ0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: June 1, 2002
Last modified: June 11, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names