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Q944C2

- PLCD5_ARATH

UniProt

Q944C2 - PLCD5_ARATH

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Protein
Phosphoinositide phospholipase C 5
Gene
PLC5, ATHATPLC6, At5g58690, MZN1.13, MZN1.15
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.1 Publication

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Calcium.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei127 – 1271 By similarity
Active sitei173 – 1731 By similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
  3. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. lipid catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciARA:AT5G58690-MONOMER.
ReactomeiREACT_187614. Synthesis of IP3 and IP4 in the cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoinositide phospholipase C 5 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase PLC5
Short name:
AtPLC5
Short name:
AtPLCx
Short name:
PI-PLC5
Gene namesi
Name:PLC5
Synonyms:ATHATPLC6
Ordered Locus Names:At5g58690
ORF Names:MZN1.13, MZN1.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G58690.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: UniProtKB-SubCell
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 578578Phosphoinositide phospholipase C 5
PRO_0000324130Add
BLAST

Proteomic databases

PRIDEiQ944C2.

Expressioni

Tissue specificityi

Expressed in leaves, roots, flowers and siliques. High levels of expression in the guard cells and vasculature of leaves and roots.1 Publication

Inductioni

By environmental stresses such as dehydration, salinity and low temperature.1 Publication

Gene expression databases

GenevestigatoriQ944C2.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G58690.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ944C2.
SMRiQ944C2. Positions 32-574.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 11184EF-hand-like
Add
BLAST
Domaini112 – 256145PI-PLC X-box
Add
BLAST
Domaini314 – 430117PI-PLC Y-box
Add
BLAST
Domaini436 – 543108C2
Add
BLAST

Sequence similaritiesi

Contains 1 C2 domain.

Phylogenomic databases

eggNOGiNOG149692.
HOGENOMiHOG000244119.
InParanoidiQ944C2.
KOiK05857.
OMAiMPEKDDF.
PhylomeDBiQ944C2.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q944C2-1 [UniParc]FASTAAdd to Basket

« Hide

MKRDMGSYKM GLCCSDKLRM NRGAPPQDVV TAFVEYTEGR SHMTAEQLCR    50
FLVEVQDETE VLVSDAEKII ERITCERHHI TKFLRHTLNL DDFFSFLFSD 100
DLNHPIDSKV HQDMASPLSH YFIYTSHNSY LTGNQINSEC SDVPLIKALK 150
RGVRALELDM WPNSTKDDIL VLHGWAWTPP VELVKCLRSI KEHAFYASAY 200
PVILTLEDHL TPDLQAKAAE MMKEIFMDMV YFPEAGGLKE FPSPEDLKYK 250
IVISTKPPKG SLRKDKDSES DASGKASSDV SADDEKTEEE TSEAKNEEDG 300
FDQESSNLDF LTYSRLITIP SGNAKNGLKE ALTIDNGGVR RLSLREQKFK 350
KATEMYGTEV IKFTQKNLLR IYPKATRVNS SNYRPYNGWM YGAQMVAFNM 400
QGYGRALWMM HGMFRGNGGC GYVKKPDFMM NNNLSGEVFN PKAKLPIKKT 450
LKVKVYMGKG WDSGFQRTCF NTWSSPNFYT RVGITGVRGD KVMKKTKKEQ 500
KTWEPFWNEE FEFQLTVPEL ALLRIEVHDY NMPEKDDFSG QTCLPVSELR 550
QGIRSVPLYD RKGERLVSVT LLMRFHFL 578
Length:578
Mass (Da):66,253
Last modified:June 1, 2002 - v2
Checksum:iA744F73545FC7364
GO

Sequence cautioni

The sequence BAA97337.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF434167 mRNA. Translation: AAL30748.2.
AB020755 Genomic DNA. Translation: BAA97337.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED97085.1.
AY062681 mRNA. Translation: AAL32759.1.
BT010399 mRNA. Translation: AAQ56842.1.
RefSeqiNP_200677.2. NM_125256.2.
UniGeneiAt.27264.

Genome annotation databases

EnsemblPlantsiAT5G58690.1; AT5G58690.1; AT5G58690.
GeneIDi835983.
KEGGiath:AT5G58690.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF434167 mRNA. Translation: AAL30748.2 .
AB020755 Genomic DNA. Translation: BAA97337.1 . Sequence problems.
CP002688 Genomic DNA. Translation: AED97085.1 .
AY062681 mRNA. Translation: AAL32759.1 .
BT010399 mRNA. Translation: AAQ56842.1 .
RefSeqi NP_200677.2. NM_125256.2.
UniGenei At.27264.

3D structure databases

ProteinModelPortali Q944C2.
SMRi Q944C2. Positions 32-574.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT5G58690.1-P.

Proteomic databases

PRIDEi Q944C2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G58690.1 ; AT5G58690.1 ; AT5G58690 .
GeneIDi 835983.
KEGGi ath:AT5G58690.

Organism-specific databases

TAIRi AT5G58690.

Phylogenomic databases

eggNOGi NOG149692.
HOGENOMi HOG000244119.
InParanoidi Q944C2.
KOi K05857.
OMAi MPEKDDF.
PhylomeDBi Q944C2.

Enzyme and pathway databases

BioCyci ARA:AT5G58690-MONOMER.
Reactomei REACT_187614. Synthesis of IP3 and IP4 in the cytosol.

Gene expression databases

Genevestigatori Q944C2.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProi IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view ]
PANTHERi PTHR10336. PTHR10336. 1 hit.
Pfami PF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view ]
PRINTSi PR00390. PHPHLIPASEC.
SMARTi SM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Preparation of polyclonal antibody specific for AtPLC4, an Arabidopsis phosphatidylinositol-specific phospholipase C in rabbits."
    Cao Z., Zhang J., Li Y., Xu X., Liu G., Bhattacharrya M.K., Yang H., Ren D.
    Protein Expr. Purif. 52:306-312(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
    DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
    Mueller-Roeber B., Pical C.
    Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. "Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms."
    Hunt L., Otterhag L., Lee J.C., Lasheen T., Hunt J., Seki M., Shinozaki K., Sommarin M., Gilmour D.J., Pical C., Gray J.E.
    New Phytol. 162:643-654(2004)
    [AGRICOLA] [Europe PMC]
    Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiPLCD5_ARATH
AccessioniPrimary (citable) accession number: Q944C2
Secondary accession number(s): Q8W4B4, Q9LUZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: June 1, 2002
Last modified: September 3, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi