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Q944C2

- PLCD5_ARATH

UniProt

Q944C2 - PLCD5_ARATH

Protein

Phosphoinositide phospholipase C 5

Gene

PLC5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 2 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.1 Publication

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

    Cofactori

    Calcium.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei127 – 1271PROSITE-ProRule annotation
    Active sitei173 – 1731PROSITE-ProRule annotation

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
    3. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. lipid catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Transducer

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Enzyme and pathway databases

    BioCyciARA:AT5G58690-MONOMER.
    ReactomeiREACT_187614. Synthesis of IP3 and IP4 in the cytosol.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoinositide phospholipase C 5 (EC:3.1.4.11)
    Alternative name(s):
    Phosphoinositide phospholipase PLC5
    Short name:
    AtPLC5
    Short name:
    AtPLCx
    Short name:
    PI-PLC5
    Gene namesi
    Name:PLC5
    Synonyms:ATHATPLC6
    Ordered Locus Names:At5g58690
    ORF Names:MZN1.13, MZN1.15
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G58690.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 578578Phosphoinositide phospholipase C 5PRO_0000324130Add
    BLAST

    Proteomic databases

    PRIDEiQ944C2.

    Expressioni

    Tissue specificityi

    Expressed in leaves, roots, flowers and siliques. High levels of expression in the guard cells and vasculature of leaves and roots.1 Publication

    Inductioni

    By environmental stresses such as dehydration, salinity and low temperature.1 Publication

    Gene expression databases

    GenevestigatoriQ944C2.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT5G58690.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ944C2.
    SMRiQ944C2. Positions 32-574.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini28 – 11184EF-hand-likeAdd
    BLAST
    Domaini112 – 256145PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini314 – 430117PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini436 – 543108C2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 EF-hand-like domain.Curated
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG149692.
    HOGENOMiHOG000244119.
    InParanoidiQ944C2.
    KOiK05857.
    OMAiMPEKDDF.
    PhylomeDBiQ944C2.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view]
    PRINTSiPR00390. PHPHLIPASEC.
    SMARTiSM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q944C2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKRDMGSYKM GLCCSDKLRM NRGAPPQDVV TAFVEYTEGR SHMTAEQLCR    50
    FLVEVQDETE VLVSDAEKII ERITCERHHI TKFLRHTLNL DDFFSFLFSD 100
    DLNHPIDSKV HQDMASPLSH YFIYTSHNSY LTGNQINSEC SDVPLIKALK 150
    RGVRALELDM WPNSTKDDIL VLHGWAWTPP VELVKCLRSI KEHAFYASAY 200
    PVILTLEDHL TPDLQAKAAE MMKEIFMDMV YFPEAGGLKE FPSPEDLKYK 250
    IVISTKPPKG SLRKDKDSES DASGKASSDV SADDEKTEEE TSEAKNEEDG 300
    FDQESSNLDF LTYSRLITIP SGNAKNGLKE ALTIDNGGVR RLSLREQKFK 350
    KATEMYGTEV IKFTQKNLLR IYPKATRVNS SNYRPYNGWM YGAQMVAFNM 400
    QGYGRALWMM HGMFRGNGGC GYVKKPDFMM NNNLSGEVFN PKAKLPIKKT 450
    LKVKVYMGKG WDSGFQRTCF NTWSSPNFYT RVGITGVRGD KVMKKTKKEQ 500
    KTWEPFWNEE FEFQLTVPEL ALLRIEVHDY NMPEKDDFSG QTCLPVSELR 550
    QGIRSVPLYD RKGERLVSVT LLMRFHFL 578
    Length:578
    Mass (Da):66,253
    Last modified:June 1, 2002 - v2
    Checksum:iA744F73545FC7364
    GO

    Sequence cautioni

    The sequence BAA97337.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF434167 mRNA. Translation: AAL30748.2.
    AB020755 Genomic DNA. Translation: BAA97337.1. Sequence problems.
    CP002688 Genomic DNA. Translation: AED97085.1.
    AY062681 mRNA. Translation: AAL32759.1.
    BT010399 mRNA. Translation: AAQ56842.1.
    RefSeqiNP_200677.2. NM_125256.2.
    UniGeneiAt.27264.

    Genome annotation databases

    EnsemblPlantsiAT5G58690.1; AT5G58690.1; AT5G58690.
    GeneIDi835983.
    KEGGiath:AT5G58690.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF434167 mRNA. Translation: AAL30748.2 .
    AB020755 Genomic DNA. Translation: BAA97337.1 . Sequence problems.
    CP002688 Genomic DNA. Translation: AED97085.1 .
    AY062681 mRNA. Translation: AAL32759.1 .
    BT010399 mRNA. Translation: AAQ56842.1 .
    RefSeqi NP_200677.2. NM_125256.2.
    UniGenei At.27264.

    3D structure databases

    ProteinModelPortali Q944C2.
    SMRi Q944C2. Positions 32-574.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT5G58690.1-P.

    Proteomic databases

    PRIDEi Q944C2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G58690.1 ; AT5G58690.1 ; AT5G58690 .
    GeneIDi 835983.
    KEGGi ath:AT5G58690.

    Organism-specific databases

    TAIRi AT5G58690.

    Phylogenomic databases

    eggNOGi NOG149692.
    HOGENOMi HOG000244119.
    InParanoidi Q944C2.
    KOi K05857.
    OMAi MPEKDDF.
    PhylomeDBi Q944C2.

    Enzyme and pathway databases

    BioCyci ARA:AT5G58690-MONOMER.
    Reactomei REACT_187614. Synthesis of IP3 and IP4 in the cytosol.

    Gene expression databases

    Genevestigatori Q944C2.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view ]
    PRINTSi PR00390. PHPHLIPASEC.
    SMARTi SM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Preparation of polyclonal antibody specific for AtPLC4, an Arabidopsis phosphatidylinositol-specific phospholipase C in rabbits."
      Cao Z., Zhang J., Li Y., Xu X., Liu G., Bhattacharrya M.K., Yang H., Ren D.
      Protein Expr. Purif. 52:306-312(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
      Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
      DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
      Mueller-Roeber B., Pical C.
      Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    6. "Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms."
      Hunt L., Otterhag L., Lee J.C., Lasheen T., Hunt J., Seki M., Shinozaki K., Sommarin M., Gilmour D.J., Pical C., Gray J.E.
      New Phytol. 162:643-654(2004)
      [AGRICOLA] [Europe PMC]
      Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiPLCD5_ARATH
    AccessioniPrimary (citable) accession number: Q944C2
    Secondary accession number(s): Q8W4B4, Q9LUZ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3