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Protein

Phosphoinositide phospholipase C 5

Gene

PLC5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.1 Publication

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei127 – 1271PROSITE-ProRule annotation
Active sitei173 – 1731PROSITE-ProRule annotation

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
  3. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. lipid catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciARA:AT5G58690-MONOMER.
ReactomeiREACT_185773. Role of phospholipids in phagocytosis.
REACT_187614. Synthesis of IP3 and IP4 in the cytosol.
REACT_187625. Signaling by FGFR1 fusion mutants.
REACT_230384. Role of second messengers in netrin-1 signaling.
REACT_231497. GPVI-mediated activation cascade.
REACT_234578. Acetylcholine regulates insulin secretion.
REACT_247278. ISG15 antiviral mechanism.
REACT_248872. DAG and IP3 signaling.
REACT_258042. G alpha (q) signalling events.
REACT_260006. Generation of second messenger molecules.
REACT_260557. VEGFR2 mediated cell proliferation.
REACT_261531. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoinositide phospholipase C 5 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase PLC5
Short name:
AtPLC5
Short name:
AtPLCx
Short name:
PI-PLC5
Gene namesi
Name:PLC5
Synonyms:ATHATPLC6
Ordered Locus Names:At5g58690
ORF Names:MZN1.13, MZN1.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G58690.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: UniProtKB-SubCell
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 578578Phosphoinositide phospholipase C 5PRO_0000324130Add
BLAST

Proteomic databases

PRIDEiQ944C2.

Expressioni

Tissue specificityi

Expressed in leaves, roots, flowers and siliques. High levels of expression in the guard cells and vasculature of leaves and roots.1 Publication

Inductioni

By environmental stresses such as dehydration, salinity and low temperature.1 Publication

Gene expression databases

GenevestigatoriQ944C2.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G58690.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ944C2.
SMRiQ944C2. Positions 32-574.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 11184EF-hand-likeAdd
BLAST
Domaini112 – 256145PI-PLC X-boxPROSITE-ProRule annotationAdd
BLAST
Domaini314 – 430117PI-PLC Y-boxPROSITE-ProRule annotationAdd
BLAST
Domaini436 – 543108C2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 EF-hand-like domain.Curated
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG149692.
HOGENOMiHOG000244119.
InParanoidiQ944C2.
KOiK05857.
OMAiMPEKDDF.
PhylomeDBiQ944C2.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q944C2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKRDMGSYKM GLCCSDKLRM NRGAPPQDVV TAFVEYTEGR SHMTAEQLCR
60 70 80 90 100
FLVEVQDETE VLVSDAEKII ERITCERHHI TKFLRHTLNL DDFFSFLFSD
110 120 130 140 150
DLNHPIDSKV HQDMASPLSH YFIYTSHNSY LTGNQINSEC SDVPLIKALK
160 170 180 190 200
RGVRALELDM WPNSTKDDIL VLHGWAWTPP VELVKCLRSI KEHAFYASAY
210 220 230 240 250
PVILTLEDHL TPDLQAKAAE MMKEIFMDMV YFPEAGGLKE FPSPEDLKYK
260 270 280 290 300
IVISTKPPKG SLRKDKDSES DASGKASSDV SADDEKTEEE TSEAKNEEDG
310 320 330 340 350
FDQESSNLDF LTYSRLITIP SGNAKNGLKE ALTIDNGGVR RLSLREQKFK
360 370 380 390 400
KATEMYGTEV IKFTQKNLLR IYPKATRVNS SNYRPYNGWM YGAQMVAFNM
410 420 430 440 450
QGYGRALWMM HGMFRGNGGC GYVKKPDFMM NNNLSGEVFN PKAKLPIKKT
460 470 480 490 500
LKVKVYMGKG WDSGFQRTCF NTWSSPNFYT RVGITGVRGD KVMKKTKKEQ
510 520 530 540 550
KTWEPFWNEE FEFQLTVPEL ALLRIEVHDY NMPEKDDFSG QTCLPVSELR
560 570
QGIRSVPLYD RKGERLVSVT LLMRFHFL
Length:578
Mass (Da):66,253
Last modified:June 1, 2002 - v2
Checksum:iA744F73545FC7364
GO

Sequence cautioni

The sequence BAA97337.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF434167 mRNA. Translation: AAL30748.2.
AB020755 Genomic DNA. Translation: BAA97337.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED97085.1.
AY062681 mRNA. Translation: AAL32759.1.
BT010399 mRNA. Translation: AAQ56842.1.
RefSeqiNP_200677.2. NM_125256.2.
UniGeneiAt.27264.

Genome annotation databases

EnsemblPlantsiAT5G58690.1; AT5G58690.1; AT5G58690.
GeneIDi835983.
KEGGiath:AT5G58690.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF434167 mRNA. Translation: AAL30748.2.
AB020755 Genomic DNA. Translation: BAA97337.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED97085.1.
AY062681 mRNA. Translation: AAL32759.1.
BT010399 mRNA. Translation: AAQ56842.1.
RefSeqiNP_200677.2. NM_125256.2.
UniGeneiAt.27264.

3D structure databases

ProteinModelPortaliQ944C2.
SMRiQ944C2. Positions 32-574.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G58690.1-P.

Proteomic databases

PRIDEiQ944C2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G58690.1; AT5G58690.1; AT5G58690.
GeneIDi835983.
KEGGiath:AT5G58690.

Organism-specific databases

TAIRiAT5G58690.

Phylogenomic databases

eggNOGiNOG149692.
HOGENOMiHOG000244119.
InParanoidiQ944C2.
KOiK05857.
OMAiMPEKDDF.
PhylomeDBiQ944C2.

Enzyme and pathway databases

BioCyciARA:AT5G58690-MONOMER.
ReactomeiREACT_185773. Role of phospholipids in phagocytosis.
REACT_187614. Synthesis of IP3 and IP4 in the cytosol.
REACT_187625. Signaling by FGFR1 fusion mutants.
REACT_230384. Role of second messengers in netrin-1 signaling.
REACT_231497. GPVI-mediated activation cascade.
REACT_234578. Acetylcholine regulates insulin secretion.
REACT_247278. ISG15 antiviral mechanism.
REACT_248872. DAG and IP3 signaling.
REACT_258042. G alpha (q) signalling events.
REACT_260006. Generation of second messenger molecules.
REACT_260557. VEGFR2 mediated cell proliferation.
REACT_261531. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.

Gene expression databases

GenevestigatoriQ944C2.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Preparation of polyclonal antibody specific for AtPLC4, an Arabidopsis phosphatidylinositol-specific phospholipase C in rabbits."
    Cao Z., Zhang J., Li Y., Xu X., Liu G., Bhattacharrya M.K., Yang H., Ren D.
    Protein Expr. Purif. 52:306-312(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
    DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
    Mueller-Roeber B., Pical C.
    Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. "Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms."
    Hunt L., Otterhag L., Lee J.C., Lasheen T., Hunt J., Seki M., Shinozaki K., Sommarin M., Gilmour D.J., Pical C., Gray J.E.
    New Phytol. 162:643-654(2004)
    [AGRICOLA] [Europe PMC]
    Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiPLCD5_ARATH
AccessioniPrimary (citable) accession number: Q944C2
Secondary accession number(s): Q8W4B4, Q9LUZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: June 1, 2002
Last modified: January 7, 2015
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.