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Protein

Phosphoinositide phospholipase C 5

Gene

PLC5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.1 Publication

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei127PROSITE-ProRule annotation1
Active sitei173PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Transducer
Biological processLipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciARA:AT5G58690-MONOMER.
BRENDAi3.1.4.11. 399.
ReactomeiR-ATH-112043. PLC beta mediated events.
R-ATH-114604. GPVI-mediated activation cascade.
R-ATH-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-ATH-202433. Generation of second messenger molecules.
R-ATH-399997. Acetylcholine regulates insulin secretion.
R-ATH-416476. G alpha (q) signalling events.
R-ATH-434316. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
R-ATH-5607764. CLEC7A (Dectin-1) signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoinositide phospholipase C 5 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase PLC5
Short name:
AtPLC5
Short name:
AtPLCx
Short name:
PI-PLC5
Gene namesi
Name:PLC5
Synonyms:ATHATPLC6
Ordered Locus Names:At5g58690
ORF Names:MZN1.13, MZN1.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRilocus:2178833. AT5G58690.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003241301 – 578Phosphoinositide phospholipase C 5Add BLAST578

Proteomic databases

PaxDbiQ944C2.

Expressioni

Tissue specificityi

Expressed in leaves, roots, flowers and siliques. High levels of expression in the guard cells and vasculature of leaves and roots.1 Publication

Inductioni

By environmental stresses such as dehydration, salinity and low temperature.1 Publication

Gene expression databases

GenevisibleiQ944C2. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G58690.1.

Structurei

3D structure databases

ProteinModelPortaliQ944C2.
SMRiQ944C2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 111EF-hand-likeAdd BLAST84
Domaini112 – 256PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST145
Domaini314 – 430PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST117
Domaini436 – 543C2PROSITE-ProRule annotationAdd BLAST108

Phylogenomic databases

eggNOGiKOG0169. Eukaryota.
ENOG410XPSW. LUCA.
HOGENOMiHOG000244119.
InParanoidiQ944C2.
KOiK05857.
OMAiMPEKDDF.
OrthoDBiEOG0936050V.
PhylomeDBiQ944C2.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 1 hit.
InterProiView protein in InterPro
IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLC_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiView protein in Pfam
PF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PRINTSiPR00390. PHPHLIPASEC.
SMARTiView protein in SMART
SM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiView protein in PROSITE
PS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Q944C2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRDMGSYKM GLCCSDKLRM NRGAPPQDVV TAFVEYTEGR SHMTAEQLCR
60 70 80 90 100
FLVEVQDETE VLVSDAEKII ERITCERHHI TKFLRHTLNL DDFFSFLFSD
110 120 130 140 150
DLNHPIDSKV HQDMASPLSH YFIYTSHNSY LTGNQINSEC SDVPLIKALK
160 170 180 190 200
RGVRALELDM WPNSTKDDIL VLHGWAWTPP VELVKCLRSI KEHAFYASAY
210 220 230 240 250
PVILTLEDHL TPDLQAKAAE MMKEIFMDMV YFPEAGGLKE FPSPEDLKYK
260 270 280 290 300
IVISTKPPKG SLRKDKDSES DASGKASSDV SADDEKTEEE TSEAKNEEDG
310 320 330 340 350
FDQESSNLDF LTYSRLITIP SGNAKNGLKE ALTIDNGGVR RLSLREQKFK
360 370 380 390 400
KATEMYGTEV IKFTQKNLLR IYPKATRVNS SNYRPYNGWM YGAQMVAFNM
410 420 430 440 450
QGYGRALWMM HGMFRGNGGC GYVKKPDFMM NNNLSGEVFN PKAKLPIKKT
460 470 480 490 500
LKVKVYMGKG WDSGFQRTCF NTWSSPNFYT RVGITGVRGD KVMKKTKKEQ
510 520 530 540 550
KTWEPFWNEE FEFQLTVPEL ALLRIEVHDY NMPEKDDFSG QTCLPVSELR
560 570
QGIRSVPLYD RKGERLVSVT LLMRFHFL
Length:578
Mass (Da):66,253
Last modified:June 1, 2002 - v2
Checksum:iA744F73545FC7364
GO

Sequence cautioni

The sequence BAA97337 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF434167 mRNA. Translation: AAL30748.2.
AB020755 Genomic DNA. Translation: BAA97337.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED97085.1.
CP002688 Genomic DNA. Translation: ANM70993.1.
AY062681 mRNA. Translation: AAL32759.1.
BT010399 mRNA. Translation: AAQ56842.1.
RefSeqiNP_001332556.1. NM_001345318.1.
NP_200677.2. NM_125256.3.
UniGeneiAt.27264.

Genome annotation databases

EnsemblPlantsiAT5G58690.1; AT5G58690.1; AT5G58690.
AT5G58690.3; AT5G58690.3; AT5G58690.
GeneIDi835983.
GrameneiAT5G58690.1; AT5G58690.1; AT5G58690.
AT5G58690.3; AT5G58690.3; AT5G58690.
KEGGiath:AT5G58690.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF434167 mRNA. Translation: AAL30748.2.
AB020755 Genomic DNA. Translation: BAA97337.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED97085.1.
CP002688 Genomic DNA. Translation: ANM70993.1.
AY062681 mRNA. Translation: AAL32759.1.
BT010399 mRNA. Translation: AAQ56842.1.
RefSeqiNP_001332556.1. NM_001345318.1.
NP_200677.2. NM_125256.3.
UniGeneiAt.27264.

3D structure databases

ProteinModelPortaliQ944C2.
SMRiQ944C2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G58690.1.

Proteomic databases

PaxDbiQ944C2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G58690.1; AT5G58690.1; AT5G58690.
AT5G58690.3; AT5G58690.3; AT5G58690.
GeneIDi835983.
GrameneiAT5G58690.1; AT5G58690.1; AT5G58690.
AT5G58690.3; AT5G58690.3; AT5G58690.
KEGGiath:AT5G58690.

Organism-specific databases

AraportiAT5G58690.
TAIRilocus:2178833. AT5G58690.

Phylogenomic databases

eggNOGiKOG0169. Eukaryota.
ENOG410XPSW. LUCA.
HOGENOMiHOG000244119.
InParanoidiQ944C2.
KOiK05857.
OMAiMPEKDDF.
OrthoDBiEOG0936050V.
PhylomeDBiQ944C2.

Enzyme and pathway databases

BioCyciARA:AT5G58690-MONOMER.
BRENDAi3.1.4.11. 399.
ReactomeiR-ATH-112043. PLC beta mediated events.
R-ATH-114604. GPVI-mediated activation cascade.
R-ATH-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-ATH-202433. Generation of second messenger molecules.
R-ATH-399997. Acetylcholine regulates insulin secretion.
R-ATH-416476. G alpha (q) signalling events.
R-ATH-434316. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
R-ATH-5607764. CLEC7A (Dectin-1) signaling.

Miscellaneous databases

PROiPR:Q944C2.

Gene expression databases

GenevisibleiQ944C2. AT.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 1 hit.
InterProiView protein in InterPro
IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLC_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiView protein in Pfam
PF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PRINTSiPR00390. PHPHLIPASEC.
SMARTiView protein in SMART
SM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiView protein in PROSITE
PS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPLCD5_ARATH
AccessioniPrimary (citable) accession number: Q944C2
Secondary accession number(s): Q8W4B4, Q9LUZ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: June 1, 2002
Last modified: April 12, 2017
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.