Q944C2 (PLCD5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoinositide phospholipase C 5
EC=3.1.4.11

Alternative name(s):
Phosphoinositide phospholipase PLC5
Short name=AtPLC5
Short name=AtPLCx
Short name=PI-PLC5

Gene names

Name:	PLC5
Synonyms:	ATHATPLC6
Ordered Locus Names:	At5g58690
ORF Names:	MZN1.13, MZN1.15

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	578 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Ref.6
Catalytic activity	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.
Cofactor	Calcium.
Subcellular location	Cytoplasm › cytosol. Cell membrane; Peripheral membrane protein.
Tissue specificity	Expressed in leaves, roots, flowers and siliques. High levels of expression in the guard cells and vasculature of leaves and roots. Ref.6
Induction	By environmental stresses such as dehydration, salinity and low temperature. Ref.6
Sequence similarities	Contains 1 C2 domain. Contains 1 EF-hand-like domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain.
Sequence caution	The sequence BAA97337.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Cell membrane Cytoplasm Membrane
Molecular function	Hydrolase Transducer
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro phosphatidylinositol phospholipase C activity Inferred from electronic annotation. Source: EC signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 578	578	Phosphoinositide phospholipase C 5		PRO_0000324130
Regions
	Domain	28 – 111	84	EF-hand-like
	Domain	112 – 256	145	PI-PLC X-box
	Domain	314 – 430	117	PI-PLC Y-box
	Domain	436 – 543	108	C2
Sites
	Active site	127	1	By similarity
	Active site	173	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q944C2 [UniParc]. Last modified June 1, 2002. Version 2. Checksum: A744F73545FC7364	FASTA	578	66,253
	10 20 30 40 50 60 MKRDMGSYKM GLCCSDKLRM NRGAPPQDVV TAFVEYTEGR SHMTAEQLCR FLVEVQDETE 70 80 90 100 110 120 VLVSDAEKII ERITCERHHI TKFLRHTLNL DDFFSFLFSD DLNHPIDSKV HQDMASPLSH 130 140 150 160 170 180 YFIYTSHNSY LTGNQINSEC SDVPLIKALK RGVRALELDM WPNSTKDDIL VLHGWAWTPP 190 200 210 220 230 240 VELVKCLRSI KEHAFYASAY PVILTLEDHL TPDLQAKAAE MMKEIFMDMV YFPEAGGLKE 250 260 270 280 290 300 FPSPEDLKYK IVISTKPPKG SLRKDKDSES DASGKASSDV SADDEKTEEE TSEAKNEEDG 310 320 330 340 350 360 FDQESSNLDF LTYSRLITIP SGNAKNGLKE ALTIDNGGVR RLSLREQKFK KATEMYGTEV 370 380 390 400 410 420 IKFTQKNLLR IYPKATRVNS SNYRPYNGWM YGAQMVAFNM QGYGRALWMM HGMFRGNGGC 430 440 450 460 470 480 GYVKKPDFMM NNNLSGEVFN PKAKLPIKKT LKVKVYMGKG WDSGFQRTCF NTWSSPNFYT 490 500 510 520 530 540 RVGITGVRGD KVMKKTKKEQ KTWEPFWNEE FEFQLTVPEL ALLRIEVHDY NMPEKDDFSG 550 560 570 QTCLPVSELR QGIRSVPLYD RKGERLVSVT LLMRFHFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Preparation of polyclonal antibody specific for AtPLC4, an Arabidopsis phosphatidylinositol-specific phospholipase C in rabbits." Cao Z., Zhang J., Li Y., Xu X., Liu G., Bhattacharrya M.K., Yang H., Ren D. Protein Expr. Purif. 52:306-312(2007) [PubMed: 17142056] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed: 10718197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C." Mueller-Roeber B., Pical C. Plant Physiol. 130:22-46(2002) [PubMed: 12226484] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[6]	"Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms." Hunt L., Otterhag L., Lee J.C., Lasheen T., Hunt J., Seki M., Shinozaki K., Sommarin M., Gilmour D.J., Pical C., Gray J.E. New Phytol. 162:643-654(2004) [Agricola: IND43668249] Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF434167 mRNA. Translation: AAL30748.2. AB020755 Genomic DNA. Translation: BAA97337.1. Sequence problems. CP002688 Genomic DNA. Translation: AED97085.1. AY062681 mRNA. Translation: AAL32759.1. BT010399 mRNA. Translation: AAQ56842.1.
IPI	IPI00543738.
RefSeq	NP_200677.2. NM_125256.2.
UniGene	At.27264.
3D structure databases
HSSP	HSSP built from PDB template 1DJH based on UniProtKB P10688.
ProteinModelPortal	Q944C2.
SMR	Q944C2. Positions 20-577.
ModBase	Search...
Protein-protein interaction databases
STRING	Q944C2.
Proteomic databases
PRIDE	Q944C2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G58690.1; AT5G58690.1; AT5G58690.
GeneID	835983.
GenomeReviews	Gene locus AT5G58690 in contig BA000015_GR.
KEGG	ath:AT5G58690.
NMPDR	fig|3702.1.peg.27853.
Organism-specific databases
TAIR	At5g58690.
Phylogenomic databases
eggNOG	KOG0169.
GeneTree	EPGT00050000005688.
HOGENOM	HBG317162.
InParanoid	Q944C2.
OMA	TIAFEII.
PhylomeDB	Q944C2.
ProtClustDB	CLSN2915010.
Gene expression databases
Genevestigator	Q944C2.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR018029. C2_membr_targeting. IPR011992. EF-hand-like_dom. IPR001192. Pinositol_PLipase_C. IPR017946. PLC-like_Pdiesterase_TIM-brl. IPR015359. PLipase_C_EF-hand-like. IPR000909. PLipase_C_PInositol-sp_X_dom. IPR001711. PLipase_C_Pinositol-sp_Y. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit. G3DSA:3.20.20.190. PLC-like_Pdiesterase_TIM-brl. 2 hits.
KO	K05857.
Pfam	PF00168. C2. 1 hit. PF09279. efhand_like. 1 hit. PF00388. PI-PLC-X. 1 hit. PF00387. PI-PLC-Y. 1 hit. [Graphical view]
PRINTS	PR00390. PHPHLIPASEC.
SMART	SM00239. C2. 1 hit. SM00148. PLCXc. 1 hit. SM00149. PLCYc. 1 hit. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 1 hit. SSF51695. PLC-like_Pdiesterase_TIM-brl. 1 hit.
PROSITE	PS50004. C2. 1 hit. PS50007. PIPLC_X_DOMAIN. 1 hit. PS50008. PIPLC_Y_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLCD5_ARATH

Accession

Primary (citable) accession number: Q944C2
Secondary accession number(s): Q8W4B4, Q9LUZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	June 1, 2002
Last modified:	December 14, 2011
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program