Phosphoinositide phospholipase C 5 - Arabidopsis thaliana (Mouse-ear cress)

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Q944C2 (PLCD5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 88. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Phosphoinositide phospholipase C 5
EC=3.1.4.11

Alternative name(s):
Phosphoinositide phospholipase PLC5
Short name=AtPLC5
Short name=AtPLCx
Short name=PI-PLC5

Gene names

Name:	PLC5
Synonyms:	ATHATPLC6
Ordered Locus Names:	At5g58690
ORF Names:	MZN1.13, MZN1.15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	578 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Ref.6
Catalytic activity	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H₂O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.
Cofactor	Calcium.
Subcellular location	Cytoplasm › cytosol. Cell membrane; Peripheral membrane protein.
Tissue specificity	Expressed in leaves, roots, flowers and siliques. High levels of expression in the guard cells and vasculature of leaves and roots. Ref.6
Induction	By environmental stresses such as dehydration, salinity and low temperature. Ref.6
Sequence similarities	Contains 1 C2 domain. Contains 1 EF-hand-like domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain.
Sequence caution	The sequence BAA97337.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism
Cellular component	Cell membrane Cytoplasm Membrane
Molecular function	Hydrolase Transducer
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro phosphatidylinositol phospholipase C activity Inferred from electronic annotation. Source: EC signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 578

578

Phosphoinositide phospholipase C 5

PRO_0000324130

Regions

Domain

28 – 111

EF-hand-like

Domain

112 – 256

145

PI-PLC X-box

Domain

314 – 430

117

PI-PLC Y-box

Domain

436 – 543

108

Sites

Active site

127

By similarity

Active site

173

By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q944C2 [UniParc].

Last modified June 1, 2002. Version 2.
Checksum: A744F73545FC7364
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FASTA

578

66,253

        10         20         30         40         50         60 
MKRDMGSYKM GLCCSDKLRM NRGAPPQDVV TAFVEYTEGR SHMTAEQLCR FLVEVQDETE 

        70         80         90        100        110        120 
VLVSDAEKII ERITCERHHI TKFLRHTLNL DDFFSFLFSD DLNHPIDSKV HQDMASPLSH 

       130        140        150        160        170        180 
YFIYTSHNSY LTGNQINSEC SDVPLIKALK RGVRALELDM WPNSTKDDIL VLHGWAWTPP 

       190        200        210        220        230        240 
VELVKCLRSI KEHAFYASAY PVILTLEDHL TPDLQAKAAE MMKEIFMDMV YFPEAGGLKE 

       250        260        270        280        290        300 
FPSPEDLKYK IVISTKPPKG SLRKDKDSES DASGKASSDV SADDEKTEEE TSEAKNEEDG 

       310        320        330        340        350        360 
FDQESSNLDF LTYSRLITIP SGNAKNGLKE ALTIDNGGVR RLSLREQKFK KATEMYGTEV 

       370        380        390        400        410        420 
IKFTQKNLLR IYPKATRVNS SNYRPYNGWM YGAQMVAFNM QGYGRALWMM HGMFRGNGGC 

       430        440        450        460        470        480 
GYVKKPDFMM NNNLSGEVFN PKAKLPIKKT LKVKVYMGKG WDSGFQRTCF NTWSSPNFYT 

       490        500        510        520        530        540 
RVGITGVRGD KVMKKTKKEQ KTWEPFWNEE FEFQLTVPEL ALLRIEVHDY NMPEKDDFSG 

       550        560        570 
QTCLPVSELR QGIRSVPLYD RKGERLVSVT LLMRFHFL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Preparation of polyclonal antibody specific for AtPLC4, an Arabidopsis phosphatidylinositol-specific phospholipase C in rabbits." Cao Z., Zhang J., Li Y., Xu X., Liu G., Bhattacharrya M.K., Yang H., Ren D. Protein Expr. Purif. 52:306-312(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C." Mueller-Roeber B., Pical C. Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[6]	"Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms." Hunt L., Otterhag L., Lee J.C., Lasheen T., Hunt J., Seki M., Shinozaki K., Sommarin M., Gilmour D.J., Pical C., Gray J.E. New Phytol. 162:643-654(2004) [AGRICOLA] [Europe PMC] Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF434167 mRNA. Translation: AAL30748.2. AB020755 Genomic DNA. Translation: BAA97337.1. Sequence problems. CP002688 Genomic DNA. Translation: AED97085.1. AY062681 mRNA. Translation: AAL32759.1. BT010399 mRNA. Translation: AAQ56842.1.
IPI	IPI00543738.
RefSeq	NP_200677.2. NM_125256.2.
UniGene	At.27264.
3D structure databases
HSSP	HSSP built from PDB template 1DJH based on UniProtKB P10688.
ProteinModelPortal	Q944C2.
SMR	Q944C2. Positions 32-574.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G58690.1-P.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G58690.1; AT5G58690.1; AT5G58690.
GeneID	835983.
KEGG	ath:AT5G58690.
Organism-specific databases
TAIR	At5g58690.
Phylogenomic databases
eggNOG	NOG149692.
HOGENOM	HOG000244119.
InParanoid	Q944C2.
KO	K05857.
OMA	MPEKDDF.
PhylomeDB	Q944C2.
ProtClustDB	CLSN2915010.
Gene expression databases
Genevestigator	Q944C2.
Family and domain databases
Gene3D	1.10.238.10. 1 hit. 3.20.20.190. 2 hits.
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR018029. C2_membr_targeting. IPR011992. EF-hand-like_dom. IPR001192. Pinositol_PLipase_C. IPR017946. PLC-like_Pdiesterase_TIM-brl. IPR015359. PLipase_C_EF-hand-like. IPR000909. PLipase_C_PInositol-sp_X_dom. IPR001711. PLipase_C_Pinositol-sp_Y. [Graphical view]
PANTHER	PTHR10336. PTHR10336. 1 hit.
Pfam	PF00168. C2. 1 hit. PF09279. efhand_like. 1 hit. PF00388. PI-PLC-X. 1 hit. PF00387. PI-PLC-Y. 1 hit. [Graphical view]
PRINTS	PR00390. PHPHLIPASEC.
SMART	SM00239. C2. 1 hit. SM00148. PLCXc. 1 hit. SM00149. PLCYc. 1 hit. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 1 hit. SSF51695. PLC-like_Pdiesterase_TIM-brl. 1 hit.
PROSITE	PS50004. C2. 1 hit. PS50007. PIPLC_X_DOMAIN. 1 hit. PS50008. PIPLC_Y_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLCD5_ARATH

Accession

Primary (citable) accession number: Q944C2
Secondary accession number(s): Q8W4B4, Q9LUZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents