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Q944C1 (PLCD4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoinositide phospholipase C 4

EC=3.1.4.11
Alternative name(s):
Phosphoinositide phospholipase PLC4
Short name=AtPLC4
Short name=PI-PLC4
Gene names
Name:PLC4
Synonyms:ATHATPLC5
Ordered Locus Names:At5g58700
ORF Names:MZN1.19
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length597 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Ref.7

Catalytic activity

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactor

Calcium.

Subcellular location

Cytoplasmcytosol. Cell membrane; Peripheral membrane protein Ref.8.

Tissue specificity

Low expression in leaves, roots, flowers and siliques. Expressed in pollen and in cells of the stigma surface. Ref.7

Induction

By environmental stresses such as dehydration, salinity and low temperature. Ref.7

Sequence similarities

Contains 1 C2 domain.

Contains 1 EF-hand domain.

Contains 1 PI-PLC X-box domain.

Contains 1 PI-PLC Y-box domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q944C1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q944C1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-108: Missing.
     109-111: ADQ → MFL
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 597597Phosphoinositide phospholipase C 4
PRO_0000324129

Regions

Domain26 – 6035EF-hand
Domain114 – 257144PI-PLC X-box
Domain333 – 449117PI-PLC Y-box
Domain455 – 562108C2

Sites

Active site1291 By similarity
Active site1741 By similarity

Natural variations

Alternative sequence1 – 108108Missing in isoform 2.
VSP_032147
Alternative sequence109 – 1113ADQ → MFL in isoform 2.
VSP_032148

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2002. Version 2.
Checksum: 176D1715653AF82B

FASTA59768,038
        10         20         30         40         50         60 
MEGKKEMGSY KFCLIFTRKF RMTESGPVED VRDLFEKYTE GDAHMSPEQL QKLMTEEGGE 

        70         80         90        100        110        120 
GETSLEEAER IVDEVLRRKH HIAKFTRRNL TLDDFNYYLF STDLNPPIAD QVHQNMDAPL 

       130        140        150        160        170        180 
SHYFIFTGHN SYLTGNQLSS NCSELPIADA LRRGVRVVEL DLWPRGTDDV CVKHGRTLTK 

       190        200        210        220        230        240 
EVKLGKCLES IKANAFAISK YPVIITLEDH LTPKLQFKVA KMITQTFGDM LYYHDSQGCQ 

       250        260        270        280        290        300 
EFPSPEELKE KILISTKPPK EYLEANDTKE KDNGEKGKDS DEDVWGKEPE DLISTQSDLD 

       310        320        330        340        350        360 
KVTSSVNDLN QDDEERGSCE SDTSCQLQAP EYKRLIAIHA GKPKGGLRMA LKVDPNKIRR 

       370        380        390        400        410        420 
LSLSEQLLEK AVASYGADVI RFTQKNFLRI YPKGTRFNSS NYKPQIGWMS GAQMIAFNMQ 

       430        440        450        460        470        480 
GYGRALWLME GMFRANGGCG YVKKPDFLMD ASPNGQDFYP KDNSSPKKTL KVKVCMGDGW 

       490        500        510        520        530        540 
LLDFKKTHFD SYSPPDFFVR VGIAGAPVDE VMEKTKIEYD TWTPIWNKEF TFPLAVPELA 

       550        560        570        580        590 
LLRVEVHEHD VNEKDDFGGQ TCLPVSEIRQ GIRAVPLFNR KGVKYSSTRL LMRFEFV 

« Hide

Isoform 2 [UniParc].

Checksum: 220C04F5089461CC
Show »

FASTA48955,420

References

« Hide 'large scale' references
[1]"Arabidopsis thaliana phosphoinositide specific phospholipase C."
Ren D., Bhattacharyya M.K.
Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[5]Pical C.
Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-597 (ISOFORM 1).
[6]"Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
Mueller-Roeber B., Pical C.
Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[7]"Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms."
Hunt L., Otterhag L., Lee J.C., Lasheen T., Hunt J., Seki M., Shinozaki K., Sommarin M., Gilmour D.J., Pical C., Gray J.E.
New Phytol. 162:643-654(2004) [AGRICOLA] [Europe PMC]
Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY.
[8]"Preparation of polyclonal antibody specific for AtPLC4, an Arabidopsis phosphatidylinositol-specific phospholipase C in rabbits."
Cao Z., Zhang J., Li Y., Xu X., Liu G., Bhattacharrya M.K., Yang H., Ren D.
Protein Expr. Purif. 52:306-312(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF434168 mRNA. Translation: AAL30749.2.
AB020755 Genomic DNA. Translation: BAA97338.1.
CP002688 Genomic DNA. Translation: AED97086.1.
CP002688 Genomic DNA. Translation: AED97087.1.
AY093217 mRNA. Translation: AAM13216.1.
BT008358 mRNA. Translation: AAP37717.1.
AY053422 mRNA. Translation: AAL23439.1.
RefSeqNP_001032097.1. NM_001037020.1. [Q944C1-2]
NP_200678.2. NM_125257.2. [Q944C1-1]
UniGeneAt.26665.

3D structure databases

ProteinModelPortalQ944C1.
SMRQ944C1. Positions 35-593.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ944C1.
PRIDEQ944C1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G58700.1; AT5G58700.1; AT5G58700. [Q944C1-1]
GeneID835984.
KEGGath:AT5G58700.

Organism-specific databases

TAIRAT5G58700.

Phylogenomic databases

eggNOGNOG149692.
HOGENOMHOG000244119.
InParanoidQ944C1.
KOK05857.
OMANERLEGP.
PhylomeDBQ944C1.

Enzyme and pathway databases

BioCycARA:AT5G58700-MONOMER.
ARA:GQT-2755-MONOMER.
BRENDA3.1.4.11. 399.

Gene expression databases

ArrayExpressQ944C1.
GenevestigatorQ944C1.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERPTHR10336. PTHR10336. 1 hit.
PfamPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSPR00390. PHPHLIPASEC.
SMARTSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePLCD4_ARATH
AccessionPrimary (citable) accession number: Q944C1
Secondary accession number(s): Q2V2X4, Q940R9, Q9LUY9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: October 1, 2002
Last modified: June 11, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names