Reviewed, UniProtKB/Swiss-Prot Q944C1 (PLCD4_ARATH)

Names and origin

Protein names

Recommended name:
    Phosphoinositide phospholipase C 4
    EC=3.1.4.11
Alternative name(s):
    Phosphoinositide phospholipase PLC4
      Short name=PI-PLC4
      Short name=AtPLC4

Gene names

Name:	PLC4
Synonyms:	ATHATPLC5
Ordered Locus Names:	At5g58700
ORF Names:	MZN1.19

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	597 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Ref.6
Catalytic activity	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.
Cofactor	Calcium.
Subcellular location	Cytoplasm › cytosol. Cell membrane; Peripheral membrane protein. Ref.7
Tissue specificity	Low expression in leaves, roots, flowers and siliques. Expressed in pollen and in cells of the stigma surface. Ref.6
Induction	By environmental stresses such as dehydration, salinity and low temperature. Ref.6
Sequence similarities	Contains 1 C2 domain. Contains 1 EF-hand domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Cell membrane Cytoplasm Membrane
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase Transducer
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	intracellular signaling cascade Inferred from electronic annotation. Source: InterPro lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	phosphoinositide phospholipase C activity Inferred from electronic annotation. Source: EC signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q944C1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q944C1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-108: Missing.
     109-111: ADQ → MFL

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 597	597	Phosphoinositide phospholipase C 4		PRO_0000324129
Regions
	Domain	26 – 60	35	EF-hand
	Domain	114 – 257	144	PI-PLC X-box
	Domain	333 – 449	117	PI-PLC Y-box
	Domain	455 – 562	108	C2
Sites
	Active site	129	1	By similarity
	Active site	174	1	By similarity
Natural variations
	Alternative sequence	1 – 108	108	Missing in isoform 2.		VSP_032147
	Alternative sequence	109 – 111	3	ADQ → MFL in isoform 2.		VSP_032148

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2002. Version 2. Checksum: 176D1715653AF82B	FASTA	597	68,038
	10 20 30 40 50 60 MEGKKEMGSY KFCLIFTRKF RMTESGPVED VRDLFEKYTE GDAHMSPEQL QKLMTEEGGE 70 80 90 100 110 120 GETSLEEAER IVDEVLRRKH HIAKFTRRNL TLDDFNYYLF STDLNPPIAD QVHQNMDAPL 130 140 150 160 170 180 SHYFIFTGHN SYLTGNQLSS NCSELPIADA LRRGVRVVEL DLWPRGTDDV CVKHGRTLTK 190 200 210 220 230 240 EVKLGKCLES IKANAFAISK YPVIITLEDH LTPKLQFKVA KMITQTFGDM LYYHDSQGCQ 250 260 270 280 290 300 EFPSPEELKE KILISTKPPK EYLEANDTKE KDNGEKGKDS DEDVWGKEPE DLISTQSDLD 310 320 330 340 350 360 KVTSSVNDLN QDDEERGSCE SDTSCQLQAP EYKRLIAIHA GKPKGGLRMA LKVDPNKIRR 370 380 390 400 410 420 LSLSEQLLEK AVASYGADVI RFTQKNFLRI YPKGTRFNSS NYKPQIGWMS GAQMIAFNMQ 430 440 450 460 470 480 GYGRALWLME GMFRANGGCG YVKKPDFLMD ASPNGQDFYP KDNSSPKKTL KVKVCMGDGW 490 500 510 520 530 540 LLDFKKTHFD SYSPPDFFVR VGIAGAPVDE VMEKTKIEYD TWTPIWNKEF TFPLAVPELA 550 560 570 580 590 LLRVEVHEHD VNEKDDFGGQ TCLPVSEIRQ GIRAVPLFNR KGVKYSSTRL LMRFEFV
	Isoform 2. Checksum: 220C04F5089461CC	FASTA	489	55,420
	10 20 30 40 50 60 MFLVHQNMDA PLSHYFIFTG HNSYLTGNQL SSNCSELPIA DALRRGVRVV ELDLWPRGTD 70 80 90 100 110 120 DVCVKHGRTL TKEVKLGKCL ESIKANAFAI SKYPVIITLE DHLTPKLQFK VAKMITQTFG 130 140 150 160 170 180 DMLYYHDSQG CQEFPSPEEL KEKILISTKP PKEYLEANDT KEKDNGEKGK DSDEDVWGKE 190 200 210 220 230 240 PEDLISTQSD LDKVTSSVND LNQDDEERGS CESDTSCQLQ APEYKRLIAI HAGKPKGGLR 250 260 270 280 290 300 MALKVDPNKI RRLSLSEQLL EKAVASYGAD VIRFTQKNFL RIYPKGTRFN SSNYKPQIGW 310 320 330 340 350 360 MSGAQMIAFN MQGYGRALWL MEGMFRANGG CGYVKKPDFL MDASPNGQDF YPKDNSSPKK 370 380 390 400 410 420 TLKVKVCMGD GWLLDFKKTH FDSYSPPDFF VRVGIAGAPV DEVMEKTKIE YDTWTPIWNK 430 440 450 460 470 480 EFTFPLAVPE LALLRVEVHE HDVNEKDDFG GQTCLPVSEI RQGIRAVPLF NRKGVKYSST RLLMRFEFV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arabidopsis thaliana phosphoinositide specific phospholipase C." Ren D., Bhattacharyya M.K. Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed: 10718197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[4]	Pical C. Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-597 (ISOFORM 1).
[5]	"Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C." Mueller-Roeber B., Pical C. Plant Physiol. 130:22-46(2002) [PubMed: 12226484] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[6]	"Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms." Hunt L., Otterhag L., Lee J.C., Lasheen T., Hunt J., Seki M., Shinozaki K., Sommarin M., Gilmour D.J., Pical C., Gray J.E. New Phytol. 162:643-654(2004) [Agricola: IND43668249] Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY.
[7]	"Preparation of polyclonal antibody specific for AtPLC4, an Arabidopsis phosphatidylinositol-specific phospholipase C in rabbits." Cao Z., Zhang J., Li Y., Xu X., Liu G., Bhattacharrya M.K., Yang H., Ren D. Protein Expr. Purif. 52:306-312(2007) [PubMed: 17142056] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF434168 mRNA. Translation: AAL30749.2. AB020755 Genomic DNA. Translation: BAA97338.1. AY093217 mRNA. Translation: AAM13216.1. BT008358 mRNA. Translation: AAP37717.1. AY053422 mRNA. Translation: AAL23439.1.
IPI	IPI00516818. IPI00656965.
RefSeq	NP_200678.2.
UniGene	At.26665
3D structure databases
HSSP	HSSP built from PDB template 1QAS based on UniProtKB P10688.
ModBase	Search...
Proteomic databases
PRIDE	Q944C1.
Genome annotation databases
GeneID	835984.
GenomeReviews	Gene locus AT5G58700 in contig BA000015_GR.
KEGG	ath:AT5G58700.
NMPDR	fig|3702.1.peg.27854.
Organism-specific databases
TAIR	At5g58700.
Phylogenomic databases
OMA	HIAKFTR.
Enzyme and pathway databases
BRENDA	3.1.4.11. 302.
Gene expression databases
ArrayExpress	Q944C1.
Genevestigator	Q944C1.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR018029. C2_membr_targeting. IPR015359. Phospholipase_C_EF-hand-like. IPR001192. Phospholipase_C_Pinositol-sp_C. IPR000909. Phospholipase_C_Pinositol-sp_X. IPR001711. Phospholipase_C_Pinositol-sp_Y. IPR017946. PLC-like_Pdiesterase_TIM-brl. [Graphical view]
Gene3D	G3DSA:3.20.20.190. PLC-like_Pdiesterase_TIM-brl. 1 hit.
Pfam	PF00168. C2. 1 hit. PF09279. efhand_like. 1 hit. PF00388. PI-PLC-X. 1 hit. PF00387. PI-PLC-Y. 1 hit. [Graphical view]
PRINTS	PR00390. PHPHLIPASEC.
ProDom	PD001202. PI_PLC_Y. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00239. C2. 1 hit. SM00148. PLCXc. 1 hit. SM00149. PLCYc. 1 hit. [Graphical view]
PROSITE	PS50004. C2. 1 hit. PS50222. EF_HAND_2. False negative. PS50007. PIPLC_X_DOMAIN. 1 hit. PS50008. PIPLC_Y_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLCD4_ARATH

Accession

Primary (citable) accession number: Q944C1
Secondary accession number(s): Q2V2X4, Q940R9, Q9LUY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	October 1, 2002
Last modified:	November 3, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)