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Protein

Phosphoinositide phospholipase C 4

Gene

PLC4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.1 Publication

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei129 – 1291PROSITE-ProRule annotation
Active sitei174 – 1741PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciARA:AT5G58700-MONOMER.
ARA:GQT-2755-MONOMER.
BRENDAi3.1.4.11. 399.
ReactomeiREACT_278077. Role of phospholipids in phagocytosis.
REACT_278842. Role of second messengers in netrin-1 signaling.
REACT_279237. VEGFR2 mediated cell proliferation.
REACT_284166. Generation of second messenger molecules.
REACT_286162. PLC beta mediated events.
REACT_306790. GPVI-mediated activation cascade.
REACT_328347. DAG and IP3 signaling.
REACT_350143. Synthesis of IP3 and IP4 in the cytosol.
REACT_361272. CLEC7A (Dectin-1) signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoinositide phospholipase C 4 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase PLC4
Short name:
AtPLC4
Short name:
PI-PLC4
Gene namesi
Name:PLC4
Synonyms:ATHATPLC5
Ordered Locus Names:At5g58700
ORF Names:MZN1.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G58700.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB-SubCell
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 597597Phosphoinositide phospholipase C 4PRO_0000324129Add
BLAST

Proteomic databases

PaxDbiQ944C1.
PRIDEiQ944C1.

Expressioni

Tissue specificityi

Low expression in leaves, roots, flowers and siliques. Expressed in pollen and in cells of the stigma surface.1 Publication

Inductioni

By environmental stresses such as dehydration, salinity and low temperature.1 Publication

Gene expression databases

ExpressionAtlasiQ944C1. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G58700.1.

Structurei

3D structure databases

ProteinModelPortaliQ944C1.
SMRiQ944C1. Positions 35-593.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 6035EF-handAdd
BLAST
Domaini114 – 257144PI-PLC X-boxPROSITE-ProRule annotationAdd
BLAST
Domaini333 – 449117PI-PLC Y-boxPROSITE-ProRule annotationAdd
BLAST
Domaini455 – 562108C2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 EF-hand domain.Curated
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG149692.
HOGENOMiHOG000244119.
InParanoidiQ944C1.
KOiK05857.
OMAiNTQQLTR.
PhylomeDBiQ944C1.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q944C1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGKKEMGSY KFCLIFTRKF RMTESGPVED VRDLFEKYTE GDAHMSPEQL
60 70 80 90 100
QKLMTEEGGE GETSLEEAER IVDEVLRRKH HIAKFTRRNL TLDDFNYYLF
110 120 130 140 150
STDLNPPIAD QVHQNMDAPL SHYFIFTGHN SYLTGNQLSS NCSELPIADA
160 170 180 190 200
LRRGVRVVEL DLWPRGTDDV CVKHGRTLTK EVKLGKCLES IKANAFAISK
210 220 230 240 250
YPVIITLEDH LTPKLQFKVA KMITQTFGDM LYYHDSQGCQ EFPSPEELKE
260 270 280 290 300
KILISTKPPK EYLEANDTKE KDNGEKGKDS DEDVWGKEPE DLISTQSDLD
310 320 330 340 350
KVTSSVNDLN QDDEERGSCE SDTSCQLQAP EYKRLIAIHA GKPKGGLRMA
360 370 380 390 400
LKVDPNKIRR LSLSEQLLEK AVASYGADVI RFTQKNFLRI YPKGTRFNSS
410 420 430 440 450
NYKPQIGWMS GAQMIAFNMQ GYGRALWLME GMFRANGGCG YVKKPDFLMD
460 470 480 490 500
ASPNGQDFYP KDNSSPKKTL KVKVCMGDGW LLDFKKTHFD SYSPPDFFVR
510 520 530 540 550
VGIAGAPVDE VMEKTKIEYD TWTPIWNKEF TFPLAVPELA LLRVEVHEHD
560 570 580 590
VNEKDDFGGQ TCLPVSEIRQ GIRAVPLFNR KGVKYSSTRL LMRFEFV
Length:597
Mass (Da):68,038
Last modified:October 1, 2002 - v2
Checksum:i176D1715653AF82B
GO
Isoform 2 (identifier: Q944C1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-108: Missing.
     109-111: ADQ → MFL

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:489
Mass (Da):55,420
Checksum:i220C04F5089461CC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 108108Missing in isoform 2. CuratedVSP_032147Add
BLAST
Alternative sequencei109 – 1113ADQ → MFL in isoform 2. CuratedVSP_032148

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF434168 mRNA. Translation: AAL30749.2.
AB020755 Genomic DNA. Translation: BAA97338.1.
CP002688 Genomic DNA. Translation: AED97086.1.
CP002688 Genomic DNA. Translation: AED97087.1.
AY093217 mRNA. Translation: AAM13216.1.
BT008358 mRNA. Translation: AAP37717.1.
AY053422 mRNA. Translation: AAL23439.1.
RefSeqiNP_001032097.1. NM_001037020.1. [Q944C1-2]
NP_200678.2. NM_125257.2. [Q944C1-1]
UniGeneiAt.26665.

Genome annotation databases

EnsemblPlantsiAT5G58700.1; AT5G58700.1; AT5G58700. [Q944C1-1]
GeneIDi835984.
KEGGiath:AT5G58700.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF434168 mRNA. Translation: AAL30749.2.
AB020755 Genomic DNA. Translation: BAA97338.1.
CP002688 Genomic DNA. Translation: AED97086.1.
CP002688 Genomic DNA. Translation: AED97087.1.
AY093217 mRNA. Translation: AAM13216.1.
BT008358 mRNA. Translation: AAP37717.1.
AY053422 mRNA. Translation: AAL23439.1.
RefSeqiNP_001032097.1. NM_001037020.1. [Q944C1-2]
NP_200678.2. NM_125257.2. [Q944C1-1]
UniGeneiAt.26665.

3D structure databases

ProteinModelPortaliQ944C1.
SMRiQ944C1. Positions 35-593.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G58700.1.

Proteomic databases

PaxDbiQ944C1.
PRIDEiQ944C1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G58700.1; AT5G58700.1; AT5G58700. [Q944C1-1]
GeneIDi835984.
KEGGiath:AT5G58700.

Organism-specific databases

TAIRiAT5G58700.

Phylogenomic databases

eggNOGiNOG149692.
HOGENOMiHOG000244119.
InParanoidiQ944C1.
KOiK05857.
OMAiNTQQLTR.
PhylomeDBiQ944C1.

Enzyme and pathway databases

BioCyciARA:AT5G58700-MONOMER.
ARA:GQT-2755-MONOMER.
BRENDAi3.1.4.11. 399.
ReactomeiREACT_278077. Role of phospholipids in phagocytosis.
REACT_278842. Role of second messengers in netrin-1 signaling.
REACT_279237. VEGFR2 mediated cell proliferation.
REACT_284166. Generation of second messenger molecules.
REACT_286162. PLC beta mediated events.
REACT_306790. GPVI-mediated activation cascade.
REACT_328347. DAG and IP3 signaling.
REACT_350143. Synthesis of IP3 and IP4 in the cytosol.
REACT_361272. CLEC7A (Dectin-1) signaling.

Miscellaneous databases

PROiQ944C1.

Gene expression databases

ExpressionAtlasiQ944C1. baseline and differential.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis thaliana phosphoinositide specific phospholipase C."
    Ren D., Bhattacharyya M.K.
    Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
    DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. Pical C.
    Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-597 (ISOFORM 1).
  6. "Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
    Mueller-Roeber B., Pical C.
    Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  7. "Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms."
    Hunt L., Otterhag L., Lee J.C., Lasheen T., Hunt J., Seki M., Shinozaki K., Sommarin M., Gilmour D.J., Pical C., Gray J.E.
    New Phytol. 162:643-654(2004)
    [AGRICOLA] [Europe PMC]
    Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY.
  8. "Preparation of polyclonal antibody specific for AtPLC4, an Arabidopsis phosphatidylinositol-specific phospholipase C in rabbits."
    Cao Z., Zhang J., Li Y., Xu X., Liu G., Bhattacharrya M.K., Yang H., Ren D.
    Protein Expr. Purif. 52:306-312(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiPLCD4_ARATH
AccessioniPrimary (citable) accession number: Q944C1
Secondary accession number(s): Q2V2X4, Q940R9, Q9LUY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: October 1, 2002
Last modified: July 22, 2015
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.