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Protein

Phosphoinositide phospholipase C 4

Gene

PLC4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.1 Publication

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei129PROSITE-ProRule annotation1
Active sitei174PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Transducer
Biological processLipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciARA:AT5G58700-MONOMER.
BRENDAi3.1.4.11. 399.
ReactomeiR-ATH-112043. PLC beta mediated events.
R-ATH-114604. GPVI-mediated activation cascade.
R-ATH-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-ATH-202433. Generation of second messenger molecules.
R-ATH-399997. Acetylcholine regulates insulin secretion.
R-ATH-416476. G alpha (q) signalling events.
R-ATH-434316. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
R-ATH-5607764. CLEC7A (Dectin-1) signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoinositide phospholipase C 4 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase PLC4
Short name:
AtPLC4
Short name:
PI-PLC4
Gene namesi
Name:PLC4
Synonyms:ATHATPLC5
Ordered Locus Names:At5g58700
ORF Names:MZN1.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G58700.
TAIRilocus:2178848. AT5G58700.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003241291 – 597Phosphoinositide phospholipase C 4Add BLAST597

Proteomic databases

PaxDbiQ944C1.

PTM databases

iPTMnetiQ944C1.

Expressioni

Tissue specificityi

Low expression in leaves, roots, flowers and siliques. Expressed in pollen and in cells of the stigma surface.1 Publication

Inductioni

By environmental stresses such as dehydration, salinity and low temperature.1 Publication

Gene expression databases

ExpressionAtlasiQ944C1. baseline and differential.
GenevisibleiQ944C1. AT.

Interactioni

Protein-protein interaction databases

BioGridi21228. 1 interactor.
STRINGi3702.AT5G58700.1.

Structurei

3D structure databases

ProteinModelPortaliQ944C1.
SMRiQ944C1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 60EF-handAdd BLAST35
Domaini114 – 257PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST144
Domaini333 – 449PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST117
Domaini455 – 562C2PROSITE-ProRule annotationAdd BLAST108

Phylogenomic databases

eggNOGiKOG0169. Eukaryota.
ENOG410XPSW. LUCA.
HOGENOMiHOG000244119.
InParanoidiQ944C1.
KOiK05857.
OMAiTEFDPIT.
OrthoDBiEOG0936050V.
PhylomeDBiQ944C1.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
3.20.20.190. 1 hit.
InterProiView protein in InterPro
IPR000008. C2_dom.
IPR035892. C2_domain_sf.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLC_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiView protein in Pfam
PF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PRINTSiPR00390. PHPHLIPASEC.
SMARTiView protein in SMART
SM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiView protein in PROSITE
PS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q944C1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGKKEMGSY KFCLIFTRKF RMTESGPVED VRDLFEKYTE GDAHMSPEQL
60 70 80 90 100
QKLMTEEGGE GETSLEEAER IVDEVLRRKH HIAKFTRRNL TLDDFNYYLF
110 120 130 140 150
STDLNPPIAD QVHQNMDAPL SHYFIFTGHN SYLTGNQLSS NCSELPIADA
160 170 180 190 200
LRRGVRVVEL DLWPRGTDDV CVKHGRTLTK EVKLGKCLES IKANAFAISK
210 220 230 240 250
YPVIITLEDH LTPKLQFKVA KMITQTFGDM LYYHDSQGCQ EFPSPEELKE
260 270 280 290 300
KILISTKPPK EYLEANDTKE KDNGEKGKDS DEDVWGKEPE DLISTQSDLD
310 320 330 340 350
KVTSSVNDLN QDDEERGSCE SDTSCQLQAP EYKRLIAIHA GKPKGGLRMA
360 370 380 390 400
LKVDPNKIRR LSLSEQLLEK AVASYGADVI RFTQKNFLRI YPKGTRFNSS
410 420 430 440 450
NYKPQIGWMS GAQMIAFNMQ GYGRALWLME GMFRANGGCG YVKKPDFLMD
460 470 480 490 500
ASPNGQDFYP KDNSSPKKTL KVKVCMGDGW LLDFKKTHFD SYSPPDFFVR
510 520 530 540 550
VGIAGAPVDE VMEKTKIEYD TWTPIWNKEF TFPLAVPELA LLRVEVHEHD
560 570 580 590
VNEKDDFGGQ TCLPVSEIRQ GIRAVPLFNR KGVKYSSTRL LMRFEFV
Length:597
Mass (Da):68,038
Last modified:October 1, 2002 - v2
Checksum:i176D1715653AF82B
GO
Isoform 2 (identifier: Q944C1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-108: Missing.
     109-111: ADQ → MFL

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:489
Mass (Da):55,420
Checksum:i220C04F5089461CC
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0321471 – 108Missing in isoform 2. CuratedAdd BLAST108
Alternative sequenceiVSP_032148109 – 111ADQ → MFL in isoform 2. Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF434168 mRNA. Translation: AAL30749.2.
AB020755 Genomic DNA. Translation: BAA97338.1.
CP002688 Genomic DNA. Translation: AED97086.1.
CP002688 Genomic DNA. Translation: AED97087.1.
CP002688 Genomic DNA. Translation: ANM70550.1.
AY093217 mRNA. Translation: AAM13216.1.
BT008358 mRNA. Translation: AAP37717.1.
AY053422 mRNA. Translation: AAL23439.1.
RefSeqiNP_001032097.1. NM_001037020.2. [Q944C1-2]
NP_001318832.1. NM_001345319.1. [Q944C1-1]
NP_200678.2. NM_125257.3. [Q944C1-1]
UniGeneiAt.26665.

Genome annotation databases

EnsemblPlantsiAT5G58700.1; AT5G58700.1; AT5G58700. [Q944C1-1]
AT5G58700.2; AT5G58700.2; AT5G58700. [Q944C1-2]
AT5G58700.3; AT5G58700.3; AT5G58700. [Q944C1-1]
GeneIDi835984.
GrameneiAT5G58700.1; AT5G58700.1; AT5G58700.
AT5G58700.2; AT5G58700.2; AT5G58700.
AT5G58700.3; AT5G58700.3; AT5G58700.
KEGGiath:AT5G58700.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPLCD4_ARATH
AccessioniPrimary (citable) accession number: Q944C1
Secondary accession number(s): Q2V2X4, Q940R9, Q9LUY9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: October 1, 2002
Last modified: October 25, 2017
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names