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Protein

Sucrose nonfermenting 4-like protein

Gene

SNF4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of the probable trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants.

GO - Molecular functioni

  • protein kinase activator activity Source: TAIR
  • protein kinase regulator activity Source: TAIR
  • protein serine/threonine kinase activity Source: TAIR

GO - Biological processi

  • carbohydrate metabolic process Source: UniProtKB-KW
  • cellular response to glucose starvation Source: TAIR
  • protein autophosphorylation Source: TAIR
  • regulation of protein kinase activity Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

ReactomeiR-ATH-163680. AMPK inhibits chREBP transcriptional activation activity.
R-ATH-200425. Import of palmitoyl-CoA into the mitochondrial matrix.
R-ATH-380972. Energy dependent regulation of mTOR by LKB1-AMPK.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.

Names & Taxonomyi

Protein namesi
Recommended name:
Sucrose nonfermenting 4-like protein
Short name:
SNF4
Alternative name(s):
CBS domain-containing protein CBSCBS3
SNF1-related protein kinase regulatory subunit betagamma
Short name:
AKIN subunit betagamma
Short name:
AKINbetagamma
Gene namesi
Name:SNF4
Synonyms:CBSCBS3
Ordered Locus Names:At1g09020
ORF Names:F7G19.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G09020.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 487487Sucrose nonfermenting 4-like proteinPRO_0000412195Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ944A6.
PRIDEiQ944A6.

PTM databases

iPTMnetiQ944A6.

Expressioni

Tissue specificityi

Preferentially expressed in dividing cells, as well as in shoot apex and flower buds, but expressed at a lower level in differentiated tissues of roots, and leaves of rosette and inflorescence.2 Publications

Gene expression databases

GenevisibleiQ944A6. AT.

Interactioni

Subunit structurei

Subunit of a probable heterotrimeric complex consisting of an alpha catalytic (KIN10 or KIN11) subunit, and a beta (KINB) and a gamma (KING or SNF4) non-catalytic regulatory subunits. Interacts with KIN10, KIN11, KINB1, KINB2, KINB3, HSPRO1 AND HSPRO2.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HSPRO2O042033EBI-2360649,EBI-1153953
KIN10Q389972EBI-2360649,EBI-2107143
KINB1Q84VQ12EBI-2360649,EBI-2042415
KINB2Q9SCY53EBI-2360649,EBI-2042436

Protein-protein interaction databases

BioGridi22664. 9 interactions.
IntActiQ944A6. 8 interactions.
STRINGi3702.AT1G09020.1.

Structurei

3D structure databases

ProteinModelPortaliQ944A6.
SMRiQ944A6. Positions 19-485.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini165 – 22763CBS 1PROSITE-ProRule annotationAdd
BLAST
Domaini260 – 32465CBS 2PROSITE-ProRule annotationAdd
BLAST
Domaini350 – 40859CBS 3PROSITE-ProRule annotationAdd
BLAST
Domaini432 – 48756CBS 4PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 10180Kinase-interacting sequence (KIS)Add
BLAST

Domaini

Kinase-interacting sequence (KIS) is required for interaction with KIN10 or KIN11.1 Publication

Sequence similaritiesi

Contains 4 CBS domains.PROSITE-ProRule annotation

Keywords - Domaini

CBS domain, Repeat

Phylogenomic databases

eggNOGiKOG1616. Eukaryota.
KOG1764. Eukaryota.
COG0517. LUCA.
HOGENOMiHOG000240774.
InParanoidiQ944A6.
OMAiRSNMDVD.
PhylomeDBiQ944A6.

Family and domain databases

InterProiIPR032640. AMPK1_CBM.
IPR000644. CBS_dom.
IPR014756. Ig_E-set.
[Graphical view]
PfamiPF16561. AMPK1_CBM. 1 hit.
PF00571. CBS. 2 hits.
[Graphical view]
SMARTiSM00116. CBS. 3 hits.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.
PROSITEiPS51371. CBS. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q944A6-1) [UniParc]FASTAAdd to basket

Also known as: AKINbetagamma

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFGSTLDSSR GNSAASGQLL TPTRFVWPYG GRRVFLSGSF TRWTEHVPMS
60 70 80 90 100
PLEGCPTVFQ VICNLTPGYH QYKFFVDGEW RHDEHQPFVS GNGGVVNTIF
110 120 130 140 150
ITGPDMVPAG FSPETLGRSN MDVDDVFLRT ADPSQEAVPR MSGVDLELSR
160 170 180 190 200
HRISVLLSTR TAYELLPESG KVIALDVNLP VKQAFHILYE QGIPLAPLWD
210 220 230 240 250
FGKGQFVGVL GPLDFILILR ELGTHGSNLT EEELETHTIA AWKEGKAHIS
260 270 280 290 300
RQYDGSGRPY PRPLVQVGPY DNLKDVALKI LQNKVAAVPV IYSSLQDGSY
310 320 330 340 350
PQLLHLASLS GILKCICRYF RHSSSSLPIL QQPICSIPLG TWVPRIGESS
360 370 380 390 400
SKPLATLRPH ASLGSALALL VQAEVSSIPV VDDNDSLIDI YSRSDITALA
410 420 430 440 450
KDKAYAQIHL DDMTVHQALQ LGQDASPPYG IFNGQRCHMC LRSDSLVKVM
460 470 480
ERLANPGVRR LVIVEAGSKR VEGIISLSDV FQFLLGL
Length:487
Mass (Da):53,466
Last modified:December 1, 2001 - v1
Checksum:i4545FE3BF2C4EBEC
GO
Isoform 2 (identifier: Q944A6-2) [UniParc]FASTAAdd to basket

Also known as: AKINbetagammaI

The sequence of this isoform differs from the canonical sequence as follows:
     394-394: S → R
     395-487: Missing.

Note: May be due to an intron retention. No experimental confirmation available.
Show »
Length:394
Mass (Da):43,302
Checksum:i4A16179AA13A1337
GO

Sequence cautioni

The sequence AAB70406.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAG10141.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei394 – 3941S → R in isoform 2. 1 PublicationVSP_041656
Alternative sequencei395 – 48793Missing in isoform 2. 1 PublicationVSP_041657Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF250335 mRNA. Translation: AAG10141.1. Different initiation.
DQ132632 mRNA. Translation: ABA12450.1.
DQ132633 mRNA. Translation: ABA12451.1.
AC000106 Genomic DNA. Translation: AAB70406.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28383.1.
AF439826 mRNA. Translation: AAL27498.1.
BT000625 mRNA. Translation: AAN18191.1.
PIRiB86222.
RefSeqiNP_563834.1. NM_100773.3. [Q944A6-1]
UniGeneiAt.10386.

Genome annotation databases

EnsemblPlantsiAT1G09020.1; AT1G09020.1; AT1G09020. [Q944A6-1]
GeneIDi837423.
KEGGiath:AT1G09020.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF250335 mRNA. Translation: AAG10141.1. Different initiation.
DQ132632 mRNA. Translation: ABA12450.1.
DQ132633 mRNA. Translation: ABA12451.1.
AC000106 Genomic DNA. Translation: AAB70406.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28383.1.
AF439826 mRNA. Translation: AAL27498.1.
BT000625 mRNA. Translation: AAN18191.1.
PIRiB86222.
RefSeqiNP_563834.1. NM_100773.3. [Q944A6-1]
UniGeneiAt.10386.

3D structure databases

ProteinModelPortaliQ944A6.
SMRiQ944A6. Positions 19-485.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22664. 9 interactions.
IntActiQ944A6. 8 interactions.
STRINGi3702.AT1G09020.1.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.

PTM databases

iPTMnetiQ944A6.

Proteomic databases

PaxDbiQ944A6.
PRIDEiQ944A6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G09020.1; AT1G09020.1; AT1G09020. [Q944A6-1]
GeneIDi837423.
KEGGiath:AT1G09020.

Organism-specific databases

TAIRiAT1G09020.

Phylogenomic databases

eggNOGiKOG1616. Eukaryota.
KOG1764. Eukaryota.
COG0517. LUCA.
HOGENOMiHOG000240774.
InParanoidiQ944A6.
OMAiRSNMDVD.
PhylomeDBiQ944A6.

Enzyme and pathway databases

ReactomeiR-ATH-163680. AMPK inhibits chREBP transcriptional activation activity.
R-ATH-200425. Import of palmitoyl-CoA into the mitochondrial matrix.
R-ATH-380972. Energy dependent regulation of mTOR by LKB1-AMPK.

Miscellaneous databases

PROiQ944A6.

Gene expression databases

GenevisibleiQ944A6. AT.

Family and domain databases

InterProiIPR032640. AMPK1_CBM.
IPR000644. CBS_dom.
IPR014756. Ig_E-set.
[Graphical view]
PfamiPF16561. AMPK1_CBM. 1 hit.
PF00571. CBS. 2 hits.
[Graphical view]
SMARTiSM00116. CBS. 3 hits.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.
PROSITEiPS51371. CBS. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Functional identification of an Arabidopsis Snf4 ortholog by screening for heterologous multicopy suppressors of snf4 deficiency in yeast."
    Kleinow T., Bhalerao R., Breuer F., Umeda M., Salchert K., Koncz C.
    Plant J. 23:115-122(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH KIN10 AND KIN11, TISSUE SPECIFICITY.
  2. "AKINbetagamma contributes to SnRK1 heterotrimeric complexes and interacts with two proteins implicated in plant pathogen resistance through its KIS/GBD sequence."
    Gissot L., Polge C., Jossier M., Girin T., Bouly J.-P., Kreis M., Thomas M.
    Plant Physiol. 142:931-944(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), ALTERNATIVE SPLICING, INTERACTION WITH KIN10; KIN11; KINB1; KINB2; KINB3; HSPRO1 AND HSPRO2, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  3. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  6. "Domain fusion between SNF1-related kinase subunits during plant evolution."
    Lumbreras V., Alba M.M., Kleinow T., Koncz C., Pages M.
    EMBO Rep. 2:55-60(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN KIS.
  7. "AKINbeta3, a plant specific SnRK1 protein, is lacking domains present in yeast and mammals non-catalytic beta-subunits."
    Gissot L., Polge C., Bouly J.P., Lemaitre T., Kreis M., Thomas M.
    Plant Mol. Biol. 56:747-759(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KINB3.
  8. "Genome wide expression analysis of CBS domain containing proteins in Arabidopsis thaliana (L.) Heynh and Oryza sativa L. reveals their developmental and stress regulation."
    Kushwaha H.R., Singh A.K., Sopory S.K., Singla-Pareek S.L., Pareek A.
    BMC Genomics 10:200-200(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  9. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSNF4_ARATH
AccessioniPrimary (citable) accession number: Q944A6
Secondary accession number(s): O04028, Q2QCL5, Q9FV59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: December 1, 2001
Last modified: July 6, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.