Q94497 (SELD_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Selenide, water dikinase
EC=2.7.9.3

Alternative name(s):
Selenium donor protein
Selenophosphate synthase

Gene names

Name:	selD
ORF Names:	DDB_G0282367

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Synthesizes selenophosphate from selenide and ATP.
Catalytic activity	ATP + selenide + H2O = AMP + selenophosphate + phosphate.
Sequence similarities	Belongs to the selenophosphate synthase 1 family. Class II subfamily.

Ontologies

Keywords
Coding sequence diversity	Selenocysteine
Ligand	ATP-binding Nucleotide-binding Selenium
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	selenocysteine metabolic process Inferred from direct assay. Source: dictyBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW selenide, water dikinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Selenide, water dikinase		PRO_0000127655
Regions
	Nucleotide binding	247 – 253	7	ATP Potential
Sites
	Active site	25	1	Potential
	Site	28	1	Important for catalytic activity
Amino acid modifications
	Non-standard residue	25	1	Selenocysteine Probable
Experimental info
	Sequence conflict	25	1	U → G in AAB17998. Ref.2
	Sequence conflict	43	1	E → G in AAB17998. Ref.2
	Sequence conflict	66	1	D → DVSLKLFKNKNKLIKLYIYI YLIYIYIFFIIFLKKK in AAB17998. Ref.2
	Sequence conflict	124	1	I → K in AAB17998. Ref.2
	Sequence conflict	180	1	K → N in AAB17998. Ref.2
	Sequence conflict	186	1	V → F in AAB17998. Ref.2
	Sequence conflict	198 – 200	3	ERW → KRG in AAB17998. Ref.2
	Sequence conflict	210	1	E → K in AAB17998. Ref.2
	Sequence conflict	225	1	R → K in AAB17998. Ref.2
	Sequence conflict	232	1	R → K in AAB17998. Ref.2
	Sequence conflict	236	1	K → N in AAB17998. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q94497 [UniParc]. Last modified February 26, 2008. Version 3. Checksum: 09DD8AA7105A8615	FASTA	364	40,552
	10 20 30 40 50 60 MSISIKDKKE ELLCRLTDFT KLKGUGCKVP QAELLSLLDG IGEGIGYDCS ISQTKFPDIV 70 80 90 100 110 120 MIQTTDFFFP LVDDPYFQGK IACANVLSDL YSFGIEDCDN MLMLLACSTD MTAEQRQWSS 130 140 150 160 170 180 KLMIQGFNDQ AICAGSKVSG GQTVKNPWPI VGGVATSILK TNEFIKPVNA VPGDVLVLTK 190 200 210 220 230 240 PLGTQVCVNF HQWLSKPERW EKINTITNAE ECEQVFNYAT LSMARLNRVG ARLMKKYNAH 250 260 270 280 290 300 AATDVTGFGI LGHSTNLAQN QLLPIRFEIH TLPIIKHMKK LEDHLNHPFK LLKGTSAETS 310 320 330 340 350 360 GGLLISMSRE NAEAFCKEIY EIEKQPAWII GDVIDQSSNY DRSKNTSIIL ENPKIIEVEP NTNF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The selD gene of Dictyostelium." Shaulsky G., Loomis W.F. Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-335. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000047 Genomic DNA. Translation: EAL66043.2. U68248 mRNA. Translation: AAB17998.1.
RefSeq	XP_640038.2. XM_634946.2.
3D structure databases
ProteinModelPortal	Q94497.
ModBase	Search...
Protein-protein interaction databases
STRING	Q94497.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0214887; DDB0214887; DDB_G0282367.
GeneID	8623564.
GenomeReviews	Gene locus selD in contig CM000152_GR.
KEGG	ddi:DDB_G0282367.
Organism-specific databases
dictyBase	DDB_G0282367. selD.
Phylogenomic databases
eggNOG	KOG3939.
HOGENOM	HBG383791.
OMA	GMDSCVI.
ProtClustDB	CLSZ2430450.
Family and domain databases
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR016188. PurM_N-like. IPR004536. SelD. [Graphical view]
KO	K01008.
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
PIRSF	PIRSF036407. Selenphspht_syn. 1 hit.
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00476. SelD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SELD_DICDI

Accession

Primary (citable) accession number: Q94497
Secondary accession number(s): Q54SK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	February 26, 2008
Last modified:	November 16, 2011
This is version 67 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)