Q94490 (UBCB_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin conjugating enzyme E2 B
EC=6.3.2.19

Alternative name(s):
UBC1

Gene names

Name:	ubcB
ORF Names:	DDB_G0284865

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	148 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in protein ubiquitination and degradation during development. Mediates protein ubiquitination at the mound and finger stage required for subsequent development and may be an essential component of the developmental transition between the induction of postaggregative gene expression and subsequent cell-type differentiation and morphogenesis. ubcB and ubpB differentially control ubiquitination/deubiquitination and degradation of mkkA protein in a cell-type-specific and temporally regulated manner. Ref.1 Ref.3
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with mkkA (via F-box/WD40 repeat domains). Ref.3
Disruption phenotype	The developmental pattern of protein ubiquitination is altered in null cells. Null cells are blocked in the ability to properly execute the developmental transition that occurs between the induction of postaggregative gene expression during mound formation and the induction of cell-type differentiation and subsequent morphogenesis. Null cells plated on agar form mounds with normal kinetics; however, they remain at this stage for 10 hours before forming multiple tips and fingers that then arrest. Postaggregative gene transcripts accumulate to very high levels and do not decrease significantly with time as they do in wild-type cells. Expression of cell-type-specific genes is very delayed, with the level of prespore-specific gene expression being significantly reduced compared with that in wild-type cells. Defect in the ability of null cells to participate in normal development in chimeras containing wild-type cells. Increased stablity of mkkA and similar phenotype (significant delay at the mound stage and arrest at the first finger stage) of cells that overexpress mkkA. Ref.1 Ref.3
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	anatomical structure morphogenesis Inferred from mutant phenotype Ref.1. Source: dictyBase
Molecular_function	ubiquitin-protein ligase activity Inferred from sequence or structural similarity Ref.1. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 148	148	Ubiquitin conjugating enzyme E2 B		PRO_0000389022
Sites
	Active site	87	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q94490 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: AAFF04D0C973C166	FASTA	148	16,809
	10 20 30 40 50 60 MAAHKRLQKE ITDMLKTPPS WCSAHLVDDN LQKWKATVQG PEGSPFEKGV FSMDIDIPAD 70 80 90 100 110 120 YPFKPPTLKF TTKIYHPNIK TSDGAICAEV FSTWSPQLKI LDVLTTIRSI LTDPNPDNPL 130 140 ETEIAQQFKT DRNAFNKTAK EWTKKYAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A ubiquitin-conjugating enzyme is essential for developmental transitions in Dictyostelium." Clark A., Nomura A., Mohanty S., Firtel R.A. Mol. Biol. Cell 8:1989-2002(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE. Strain: AX3.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"A novel, putative MEK kinase controls developmental timing and spatial patterning in Dictyostelium and is regulated by ubiquitin-mediated protein degradation." Chung C.Y., Reddy T.B.K., Zhou K., Firtel R.A. Genes Dev. 12:3564-3578(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH MKKA, DISRUPTION PHENOTYPE. Strain: AX3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U67838 Genomic DNA. Translation: AAB08700.1. AAFI02000073 Genomic DNA. Translation: EAL64896.1.
RefSeq	XP_639901.1. XM_634809.1.
3D structure databases
HSSP	HSSP built from PDB template 1Z2U based on UniProtKB P35129.
ProteinModelPortal	Q94490.
ModBase	Search...
Proteomic databases
PRIDE	Q94490.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0201564; DDB0201564; DDB_G0284865.
GeneID	8624813.
KEGG	ddi:DDB_G0284865.
Organism-specific databases
dictyBase	DDB_G0284865. ubcB.
Phylogenomic databases
eggNOG	COG5078.
InParanoid	Q94490.
OMA	ICAEVFS.
Enzyme and pathway databases
UniPathway	UPA00143.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. False negative. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBCB_DICDI

Accession

Primary (citable) accession number: Q94490
Secondary accession number(s): Q54P16

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 24, 2009
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)