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Q94490 (UBCB_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin conjugating enzyme E2 B

EC=6.3.2.19
Alternative name(s):
UBC1
Gene names
Name:ubcB
ORF Names:DDB_G0284865
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length148 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in protein ubiquitination and degradation during development. Mediates protein ubiquitination at the mound and finger stage required for subsequent development and may be an essential component of the developmental transition between the induction of postaggregative gene expression and subsequent cell-type differentiation and morphogenesis. ubcB and ubpB differentially control ubiquitination/deubiquitination and degradation of mkkA protein in a cell-type-specific and temporally regulated manner. Ref.1 Ref.3

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with mkkA (via F-box/WD40 repeat domains). Ref.3

Disruption phenotype

The developmental pattern of protein ubiquitination is altered in null cells. Null cells are blocked in the ability to properly execute the developmental transition that occurs between the induction of postaggregative gene expression during mound formation and the induction of cell-type differentiation and subsequent morphogenesis. Null cells plated on agar form mounds with normal kinetics; however, they remain at this stage for 10 hours before forming multiple tips and fingers that then arrest. Postaggregative gene transcripts accumulate to very high levels and do not decrease significantly with time as they do in wild-type cells. Expression of cell-type-specific genes is very delayed, with the level of prespore-specific gene expression being significantly reduced compared with that in wild-type cells. Defect in the ability of null cells to participate in normal development in chimeras containing wild-type cells. Increased stablity of mkkA and similar phenotype (significant delay at the mound stage and arrest at the first finger stage) of cells that overexpress mkkA. Ref.1 Ref.3

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processanatomical structure morphogenesis

Inferred from mutant phenotype Ref.1. Source: dictyBase

protein ubiquitination

Inferred from direct assay Ref.3. Source: dictyBase

   Molecular_functionubiquitin-protein transferase activity

Inferred from sequence or structural similarity Ref.1. Source: dictyBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 148148Ubiquitin conjugating enzyme E2 B
PRO_0000389022

Sites

Active site871Glycyl thioester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q94490 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: AAFF04D0C973C166

FASTA14816,809
        10         20         30         40         50         60 
MAAHKRLQKE ITDMLKTPPS WCSAHLVDDN LQKWKATVQG PEGSPFEKGV FSMDIDIPAD 

        70         80         90        100        110        120 
YPFKPPTLKF TTKIYHPNIK TSDGAICAEV FSTWSPQLKI LDVLTTIRSI LTDPNPDNPL 

       130        140 
ETEIAQQFKT DRNAFNKTAK EWTKKYAK 

« Hide

References

« Hide 'large scale' references
[1]"A ubiquitin-conjugating enzyme is essential for developmental transitions in Dictyostelium."
Clark A., Nomura A., Mohanty S., Firtel R.A.
Mol. Biol. Cell 8:1989-2002(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE.
Strain: AX3.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"A novel, putative MEK kinase controls developmental timing and spatial patterning in Dictyostelium and is regulated by ubiquitin-mediated protein degradation."
Chung C.Y., Reddy T.B.K., Zhou K., Firtel R.A.
Genes Dev. 12:3564-3578(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH MKKA, DISRUPTION PHENOTYPE.
Strain: AX3.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U67838 Genomic DNA. Translation: AAB08700.1.
AAFI02000073 Genomic DNA. Translation: EAL64896.1.
RefSeqXP_639901.1. XM_634809.1.

3D structure databases

ProteinModelPortalQ94490.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0201564.

Proteomic databases

PRIDEQ94490.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0201564; DDB0201564; DDB_G0284865.
GeneID8624813.
KEGGddi:DDB_G0284865.

Organism-specific databases

dictyBaseDDB_G0284865. ubcB.

Phylogenomic databases

eggNOGCOG5078.
InParanoidQ94490.
OMARPECEIA.
PhylomeDBQ94490.

Enzyme and pathway databases

UniPathwayUPA00143.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBCB_DICDI
AccessionPrimary (citable) accession number: Q94490
Secondary accession number(s): Q54P16
Entry history
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase