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Q94490

- UBCB_DICDI

UniProt

Q94490 - UBCB_DICDI

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Protein

Ubiquitin conjugating enzyme E2 B

Gene

ubcB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Involved in protein ubiquitination and degradation during development. Mediates protein ubiquitination at the mound and finger stage required for subsequent development and may be an essential component of the developmental transition between the induction of postaggregative gene expression and subsequent cell-type differentiation and morphogenesis. ubcB and ubpB differentially control ubiquitination/deubiquitination and degradation of mkkA protein in a cell-type-specific and temporally regulated manner.2 Publications

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei87 – 871Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ubiquitin-protein transferase activity Source: dictyBase

GO - Biological processi

  1. anatomical structure morphogenesis Source: dictyBase
  2. protein ubiquitination Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin conjugating enzyme E2 B (EC:6.3.2.19)
Alternative name(s):
UBC1
Gene namesi
Name:ubcB
ORF Names:DDB_G0284865
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0284865. ubcB.

Pathology & Biotechi

Disruption phenotypei

The developmental pattern of protein ubiquitination is altered in null cells. Null cells are blocked in the ability to properly execute the developmental transition that occurs between the induction of postaggregative gene expression during mound formation and the induction of cell-type differentiation and subsequent morphogenesis. Null cells plated on agar form mounds with normal kinetics; however, they remain at this stage for 10 hours before forming multiple tips and fingers that then arrest. Postaggregative gene transcripts accumulate to very high levels and do not decrease significantly with time as they do in wild-type cells. Expression of cell-type-specific genes is very delayed, with the level of prespore-specific gene expression being significantly reduced compared with that in wild-type cells. Defect in the ability of null cells to participate in normal development in chimeras containing wild-type cells. Increased stablity of mkkA and similar phenotype (significant delay at the mound stage and arrest at the first finger stage) of cells that overexpress mkkA.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 148148Ubiquitin conjugating enzyme E2 BPRO_0000389022Add
BLAST

Proteomic databases

PRIDEiQ94490.

Interactioni

Subunit structurei

Interacts with mkkA (via F-box/WD40 repeat domains).1 Publication

Protein-protein interaction databases

STRINGi44689.DDB_0201564.

Structurei

3D structure databases

ProteinModelPortaliQ94490.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
InParanoidiQ94490.
OMAiRPECEIA.
PhylomeDBiQ94490.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q94490-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAHKRLQKE ITDMLKTPPS WCSAHLVDDN LQKWKATVQG PEGSPFEKGV
60 70 80 90 100
FSMDIDIPAD YPFKPPTLKF TTKIYHPNIK TSDGAICAEV FSTWSPQLKI
110 120 130 140
LDVLTTIRSI LTDPNPDNPL ETEIAQQFKT DRNAFNKTAK EWTKKYAK
Length:148
Mass (Da):16,809
Last modified:February 1, 1997 - v1
Checksum:iAAFF04D0C973C166
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U67838 Genomic DNA. Translation: AAB08700.1.
AAFI02000073 Genomic DNA. Translation: EAL64896.1.
RefSeqiXP_639901.1. XM_634809.1.

Genome annotation databases

EnsemblProtistsiDDB0201564; DDB0201564; DDB_G0284865.
GeneIDi8624813.
KEGGiddi:DDB_G0284865.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U67838 Genomic DNA. Translation: AAB08700.1 .
AAFI02000073 Genomic DNA. Translation: EAL64896.1 .
RefSeqi XP_639901.1. XM_634809.1.

3D structure databases

ProteinModelPortali Q94490.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0201564.

Proteomic databases

PRIDEi Q94490.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0201564 ; DDB0201564 ; DDB_G0284865 .
GeneIDi 8624813.
KEGGi ddi:DDB_G0284865.

Organism-specific databases

dictyBasei DDB_G0284865. ubcB.

Phylogenomic databases

eggNOGi COG5078.
InParanoidi Q94490.
OMAi RPECEIA.
PhylomeDBi Q94490.

Enzyme and pathway databases

UniPathwayi UPA00143 .

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A ubiquitin-conjugating enzyme is essential for developmental transitions in Dictyostelium."
    Clark A., Nomura A., Mohanty S., Firtel R.A.
    Mol. Biol. Cell 8:1989-2002(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE.
    Strain: AX3.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "A novel, putative MEK kinase controls developmental timing and spatial patterning in Dictyostelium and is regulated by ubiquitin-mediated protein degradation."
    Chung C.Y., Reddy T.B.K., Zhou K., Firtel R.A.
    Genes Dev. 12:3564-3578(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MKKA, DISRUPTION PHENOTYPE.
    Strain: AX3.

Entry informationi

Entry nameiUBCB_DICDI
AccessioniPrimary (citable) accession number: Q94490
Secondary accession number(s): Q54P16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: February 1, 1997
Last modified: October 1, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3