Q94490 (UBCB_DICDI) Reviewed, UniProtKB/Swiss-Prot
Last modified May 1, 2013. Version 78. History...
Names and origin
|Protein names||Recommended name:|
Ubiquitin conjugating enzyme E2 B
|Organism||Dictyostelium discoideum (Slime mold) [Reference proteome]|
|Taxonomic identifier||44689 [NCBI]|
|Taxonomic lineage||Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium|
|Sequence length||148 AA.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
Involved in protein ubiquitination and degradation during development. Mediates protein ubiquitination at the mound and finger stage required for subsequent development and may be an essential component of the developmental transition between the induction of postaggregative gene expression and subsequent cell-type differentiation and morphogenesis. ubcB and ubpB differentially control ubiquitination/deubiquitination and degradation of mkkA protein in a cell-type-specific and temporally regulated manner. Ref.1 Ref.3
ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Interacts with mkkA (via F-box/WD40 repeat domains). Ref.3
The developmental pattern of protein ubiquitination is altered in null cells. Null cells are blocked in the ability to properly execute the developmental transition that occurs between the induction of postaggregative gene expression during mound formation and the induction of cell-type differentiation and subsequent morphogenesis. Null cells plated on agar form mounds with normal kinetics; however, they remain at this stage for 10 hours before forming multiple tips and fingers that then arrest. Postaggregative gene transcripts accumulate to very high levels and do not decrease significantly with time as they do in wild-type cells. Expression of cell-type-specific genes is very delayed, with the level of prespore-specific gene expression being significantly reduced compared with that in wild-type cells. Defect in the ability of null cells to participate in normal development in chimeras containing wild-type cells. Increased stablity of mkkA and similar phenotype (significant delay at the mound stage and arrest at the first finger stage) of cells that overexpress mkkA. Ref.1 Ref.3
Belongs to the ubiquitin-conjugating enzyme family.
|Biological process||Ubl conjugation pathway|
|Technical term||Complete proteome|
|Gene Ontology (GO)|
|Biological_process||anatomical structure morphogenesis|
|Molecular_function||ubiquitin-protein ligase activity|
|Complete GO annotation...|
Sequence annotation (Features)
|||"A ubiquitin-conjugating enzyme is essential for developmental transitions in Dictyostelium."|
Clark A., Nomura A., Mohanty S., Firtel R.A.
Mol. Biol. Cell 8:1989-2002(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE.
|||"The genome of the social amoeba Dictyostelium discoideum."|
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
|||"A novel, putative MEK kinase controls developmental timing and spatial patterning in Dictyostelium and is regulated by ubiquitin-mediated protein degradation."|
Chung C.Y., Reddy T.B.K., Zhou K., Firtel R.A.
Genes Dev. 12:3564-3578(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH MKKA, DISRUPTION PHENOTYPE.
|+||Additional computationally mapped references.|
|U67838 Genomic DNA. Translation: AAB08700.1.|
AAFI02000073 Genomic DNA. Translation: EAL64896.1.
|RefSeq||XP_639901.1. XM_634809.1. |
3D structure databases
|HSSP||HSSP built from PDB template 1Z2U based on UniProtKB P35129. |
Protocols and materials databases
Genome annotation databases
|EnsemblProtists||DDB0201564; DDB0201564; DDB_G0284865. |
|dictyBase||DDB_G0284865. ubcB. |
Enzyme and pathway databases
Family and domain databases
|Gene3D||220.127.116.11. 1 hit. |
|InterPro||IPR000608. UBQ-conjugat_E2. |
|Pfam||PF00179. UQ_con. 1 hit. |
|SUPFAM||SSF54495. UBQ-conjugat/RWD-like. 1 hit. |
|PROSITE||PS00183. UBIQUITIN_CONJUGAT_1. False negative. |
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
|Accession||Primary (citable) accession number: Q94490|
Secondary accession number(s): Q54P16
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|