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Protein

Ubiquitin conjugating enzyme E2 B

Gene

ubcB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in protein ubiquitination and degradation during development. Mediates protein ubiquitination at the mound and finger stage required for subsequent development and may be an essential component of the developmental transition between the induction of postaggregative gene expression and subsequent cell-type differentiation and morphogenesis. ubcB and ubpB differentially control ubiquitination/deubiquitination and degradation of mkkA protein in a cell-type-specific and temporally regulated manner.2 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei87Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ubiquitin-protein transferase activity Source: dictyBase

GO - Biological processi

  • anatomical structure morphogenesis Source: dictyBase
  • protein ubiquitination Source: dictyBase

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin conjugating enzyme E2 B (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme B
UBC1
Gene namesi
Name:ubcB
ORF Names:DDB_G0284865
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0284865 ubcB

Pathology & Biotechi

Disruption phenotypei

The developmental pattern of protein ubiquitination is altered in null cells. Null cells are blocked in the ability to properly execute the developmental transition that occurs between the induction of postaggregative gene expression during mound formation and the induction of cell-type differentiation and subsequent morphogenesis. Null cells plated on agar form mounds with normal kinetics; however, they remain at this stage for 10 hours before forming multiple tips and fingers that then arrest. Postaggregative gene transcripts accumulate to very high levels and do not decrease significantly with time as they do in wild-type cells. Expression of cell-type-specific genes is very delayed, with the level of prespore-specific gene expression being significantly reduced compared with that in wild-type cells. Defect in the ability of null cells to participate in normal development in chimeras containing wild-type cells. Increased stablity of mkkA and similar phenotype (significant delay at the mound stage and arrest at the first finger stage) of cells that overexpress mkkA.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003890221 – 148Ubiquitin conjugating enzyme E2 BAdd BLAST148

Proteomic databases

PaxDbiQ94490

Interactioni

Subunit structurei

Interacts with mkkA (via F-box/WD40 repeat domains).1 Publication

Protein-protein interaction databases

STRINGi44689.DDB0201564

Structurei

3D structure databases

ProteinModelPortaliQ94490
SMRiQ94490
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417 Eukaryota
COG5078 LUCA
InParanoidiQ94490
OMAiHCNIANG
PhylomeDBiQ94490

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q94490-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAHKRLQKE ITDMLKTPPS WCSAHLVDDN LQKWKATVQG PEGSPFEKGV
60 70 80 90 100
FSMDIDIPAD YPFKPPTLKF TTKIYHPNIK TSDGAICAEV FSTWSPQLKI
110 120 130 140
LDVLTTIRSI LTDPNPDNPL ETEIAQQFKT DRNAFNKTAK EWTKKYAK
Length:148
Mass (Da):16,809
Last modified:February 1, 1997 - v1
Checksum:iAAFF04D0C973C166
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U67838 Genomic DNA Translation: AAB08700.1
AAFI02000073 Genomic DNA Translation: EAL64896.1
RefSeqiXP_639901.1, XM_634809.1

Genome annotation databases

EnsemblProtistsiEAL64896; EAL64896; DDB_G0284865
GeneIDi8624813
KEGGiddi:DDB_G0284865

Similar proteinsi

Entry informationi

Entry nameiUBCB_DICDI
AccessioniPrimary (citable) accession number: Q94490
Secondary accession number(s): Q54P16
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health