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Protein

Glyceraldehyde-3-phosphate dehydrogenase

Gene

gpdA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gpdA)
  2. Phosphoglycerate kinase (pgkA)
  3. no protein annotated in this organism
  4. Enolase A (enoA), Enolase B (enoB)
  5. Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei34 – 341NADBy similarity
Binding sitei79 – 791NAD; via carbonyl oxygenBy similarity
Active sitei151 – 1511NucleophilePROSITE-ProRule annotation
Sitei178 – 1781Activates thiol group during catalysisBy similarity
Binding sitei181 – 1811Glyceraldehyde 3-phosphateBy similarity
Binding sitei233 – 2331Glyceraldehyde 3-phosphateBy similarity
Binding sitei316 – 3161NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 132NADBy similarity

GO - Molecular functioni

GO - Biological processi

  • gluconeogenesis Source: dictyBase
  • glycolytic process Source: dictyBase
  • response to reactive oxygen species Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-DDI-70171. Glycolysis.
R-DDI-70263. Gluconeogenesis.
UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase (EC:1.2.1.12)
Short name:
GAPDH
Gene namesi
Name:gpdA
ORF Names:DDB_G0275153
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 2, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0275153. gpdA.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: dictyBase
  • phagocytic vesicle Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 335334Glyceraldehyde-3-phosphate dehydrogenasePRO_0000145514Add
BLAST

Proteomic databases

PaxDbiQ94469.
PRIDEiQ94469.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0185087.

Structurei

3D structure databases

ProteinModelPortaliQ94469.
SMRiQ94469. Positions 3-334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni150 – 1523Glyceraldehyde 3-phosphate bindingBy similarity
Regioni210 – 2112Glyceraldehyde 3-phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.
InParanoidiQ94469.
KOiK00134.
OMAiICHIASA.
PhylomeDBiQ94469.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q94469-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVPIGINGF GRIGRLVLRA SLENPECRVL AINEPFMDVK YMVYMFKYDS
60 70 80 90 100
THGRFKGTVE DINGEFVVNG NKIHVFAEKD PANIKWSSVG AEYIVESTGL
110 120 130 140 150
FLSTEKAGVH LKGGAKKVVI SAPSTDAPMY VMGVNEETYE SSHDVISNAS
160 170 180 190 200
CTTNCLAPLA KIIHENFGIV EGLMTTVHAI TATQKTVDGP SGKDWRAGRS
210 220 230 240 250
ALSNIIPAST GAAKAVGKVL PALNGKLTGM SFRVPNCDVS VVDLTVRLEK
260 270 280 290 300
KATYEEIKKV MKAASESDKY KRYIGYTEDE VVSTDFIGDT HSSIFDAHAG
310 320 330
IALNDNFVKL VSWYDNEMGY STRVIDLLVY ISKKN
Length:335
Mass (Da):36,552
Last modified:December 4, 2007 - v2
Checksum:i58CCC779D16CDF5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000013 Genomic DNA. Translation: EAL69857.1.
U55243 Genomic DNA. Translation: AAC47285.1.
RefSeqiXP_643857.1. XM_638765.1.

Genome annotation databases

EnsemblProtistsiDDB0185087; DDB0185087; DDB_G0275153.
GeneIDi8619908.
KEGGiddi:DDB_G0275153.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000013 Genomic DNA. Translation: EAL69857.1.
U55243 Genomic DNA. Translation: AAC47285.1.
RefSeqiXP_643857.1. XM_638765.1.

3D structure databases

ProteinModelPortaliQ94469.
SMRiQ94469. Positions 3-334.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0185087.

Proteomic databases

PaxDbiQ94469.
PRIDEiQ94469.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0185087; DDB0185087; DDB_G0275153.
GeneIDi8619908.
KEGGiddi:DDB_G0275153.

Organism-specific databases

dictyBaseiDDB_G0275153. gpdA.

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.
InParanoidiQ94469.
KOiK00134.
OMAiICHIASA.
PhylomeDBiQ94469.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.
ReactomeiR-DDI-70171. Glycolysis.
R-DDI-70263. Gluconeogenesis.

Miscellaneous databases

PROiQ94469.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3P_DICDI
AccessioniPrimary (citable) accession number: Q94469
Secondary accession number(s): Q553V4, Q869Y9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: December 4, 2007
Last modified: September 7, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.