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Protein

Glyceraldehyde-3-phosphate dehydrogenase

Gene

gpdA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gpdA)
  2. Phosphoglycerate kinase (pgkA), Phosphoglycerate kinase (pgkA)
  3. no protein annotated in this organism
  4. Enolase A (enoA), Enolase B (enoB)
  5. Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34NADBy similarity1
Binding sitei79NAD; via carbonyl oxygenBy similarity1
Active sitei151NucleophilePROSITE-ProRule annotation1
Sitei178Activates thiol group during catalysisBy similarity1
Binding sitei181Glyceraldehyde 3-phosphateBy similarity1
Binding sitei233Glyceraldehyde 3-phosphateBy similarity1
Binding sitei316NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 13NADBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolysis
LigandNAD

Enzyme and pathway databases

ReactomeiR-DDI-70171 Glycolysis
R-DDI-70263 Gluconeogenesis
UniPathwayiUPA00109; UER00184

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase (EC:1.2.1.12)
Short name:
GAPDH
Gene namesi
Name:gpdA
ORF Names:DDB_G0275153
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 2, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0275153 gpdA

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001455142 – 335Glyceraldehyde-3-phosphate dehydrogenaseAdd BLAST334

Proteomic databases

PaxDbiQ94469
PRIDEiQ94469

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0185087

Structurei

3D structure databases

ProteinModelPortaliQ94469
SMRiQ94469
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni150 – 152Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni210 – 211Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0657 Eukaryota
COG0057 LUCA
InParanoidiQ94469
KOiK00134
OMAiFTLENMV
PhylomeDBiQ94469

Family and domain databases

InterProiView protein in InterPro
IPR020831 GlycerAld/Erythrose_P_DH
IPR020830 GlycerAld_3-P_DH_AS
IPR020829 GlycerAld_3-P_DH_cat
IPR020828 GlycerAld_3-P_DH_NAD(P)-bd
IPR006424 Glyceraldehyde-3-P_DH_1
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR10836 PTHR10836, 1 hit
PfamiView protein in Pfam
PF02800 Gp_dh_C, 1 hit
PF00044 Gp_dh_N, 1 hit
PIRSFiPIRSF000149 GAP_DH, 1 hit
PRINTSiPR00078 G3PDHDRGNASE
SMARTiView protein in SMART
SM00846 Gp_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR01534 GAPDH-I, 1 hit
PROSITEiView protein in PROSITE
PS00071 GAPDH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q94469-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVPIGINGF GRIGRLVLRA SLENPECRVL AINEPFMDVK YMVYMFKYDS
60 70 80 90 100
THGRFKGTVE DINGEFVVNG NKIHVFAEKD PANIKWSSVG AEYIVESTGL
110 120 130 140 150
FLSTEKAGVH LKGGAKKVVI SAPSTDAPMY VMGVNEETYE SSHDVISNAS
160 170 180 190 200
CTTNCLAPLA KIIHENFGIV EGLMTTVHAI TATQKTVDGP SGKDWRAGRS
210 220 230 240 250
ALSNIIPAST GAAKAVGKVL PALNGKLTGM SFRVPNCDVS VVDLTVRLEK
260 270 280 290 300
KATYEEIKKV MKAASESDKY KRYIGYTEDE VVSTDFIGDT HSSIFDAHAG
310 320 330
IALNDNFVKL VSWYDNEMGY STRVIDLLVY ISKKN
Length:335
Mass (Da):36,552
Last modified:December 4, 2007 - v2
Checksum:i58CCC779D16CDF5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000013 Genomic DNA Translation: EAL69857.1
U55243 Genomic DNA Translation: AAC47285.1
RefSeqiXP_643857.1, XM_638765.1

Genome annotation databases

EnsemblProtistsiEAL69857; EAL69857; DDB_G0275153
GeneIDi8619908
KEGGiddi:DDB_G0275153

Similar proteinsi

Entry informationi

Entry nameiG3P_DICDI
AccessioniPrimary (citable) accession number: Q94469
Secondary accession number(s): Q553V4, Q869Y9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: December 4, 2007
Last modified: April 25, 2018
This is version 123 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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