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Q94465

- GCH1_DICDI

UniProt

Q94465 - GCH1_DICDI

Protein

GTP cyclohydrolase 1

Gene

gchA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 2 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi121 – 1211ZincBy similarity
    Metal bindingi124 – 1241ZincBy similarity
    Metal bindingi192 – 1921ZincBy similarity

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. GTP cyclohydrolase I activity Source: dictyBase
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. 7,8-dihydroneopterin 3'-triphosphate biosynthetic process Source: UniProtKB-UniPathway
    2. regulation of G-protein coupled receptor protein signaling pathway Source: dictyBase
    3. response to oxidative stress Source: dictyBase
    4. tetrahydrobiopterin biosynthetic process Source: dictyBase
    5. tetrahydrofolate biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Tetrahydrobiopterin biosynthesis

    Keywords - Ligandi

    GTP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_212091. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
    UniPathwayiUPA00848; UER00151.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTP cyclohydrolase 1 (EC:3.5.4.16)
    Alternative name(s):
    GTP cyclohydrolase I
    Short name:
    GTP-CH-I
    Gene namesi
    Name:gchA
    Synonyms:folE, gtoC
    ORF Names:DDB_G0288481
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0288481. gchA.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: dictyBase
    2. membrane Source: dictyBase
    3. phagocytic vesicle Source: dictyBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 232232GTP cyclohydrolase 1PRO_0000119481Add
    BLAST

    Proteomic databases

    PRIDEiQ94465.

    Interactioni

    Subunit structurei

    Toroid-shaped homodecamer, composed of two pentamers of five dimers.By similarity

    Protein-protein interaction databases

    STRINGi44689.DDB_0191399.

    Structurei

    3D structure databases

    ProteinModelPortaliQ94465.
    SMRiQ94465. Positions 48-230.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GTP cyclohydrolase I family.Curated

    Phylogenomic databases

    eggNOGiCOG0302.
    KOiK01495.
    OMAiVYSHCEH.
    PhylomeDBiQ94465.

    Family and domain databases

    HAMAPiMF_00223. FolE.
    InterProiIPR001474. GTP_CycHdrlase_I.
    IPR018234. GTP_CycHdrlase_I_CS.
    IPR020602. GTP_CycHdrlase_I_dom.
    [Graphical view]
    PANTHERiPTHR11109. PTHR11109. 1 hit.
    PfamiPF01227. GTP_cyclohydroI. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00063. folE. 1 hit.
    PROSITEiPS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
    PS00860. GTP_CYCLOHYDROL_1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q94465-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDNLKSYQD NHIENEDEEI YERSNGKGKE LVDFGKKREP LIHNHEVLNT    50
    MQSSVKTLLS SLGEDPDREG LLKTPLRMSK ALLFFTQGYE QSVDEVIGEA 100
    IFNENHHEMV VVRDIDIFSL CEHHMVPFHG KCHIGYIPDQ KVLGLSKLAR 150
    VAEIFARRLQ VQERLTRQIA QAIQAHLNPM GVAVVIEASH MCMVMRGVQK 200
    PGASTVTSSV CGIFEKDSRT RAEFFSLIKS NK 232
    Length:232
    Mass (Da):26,277
    Last modified:December 4, 2007 - v2
    Checksum:iD36603D793EE5EA2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti187 – 1871E → D in CAA89808. (PubMed:8870645)Curated
    Sequence conflicti206 – 2061V → A in CAA89808. (PubMed:8870645)Curated
    Sequence conflicti216 – 2161K → E in CAA89808. (PubMed:8870645)Curated
    Sequence conflicti228 – 2325IKSNK → FNSTN in CAA89808. (PubMed:8870645)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49706 mRNA. Translation: CAA89808.1.
    AAFI02000112 Genomic DNA. Translation: EAL63189.1.
    PIRiS72439.
    RefSeqiXP_636697.1. XM_631605.1.

    Genome annotation databases

    EnsemblProtistsiDDB0191399; DDB0191399; DDB_G0288481.
    GeneIDi8626654.
    KEGGiddi:DDB_G0288481.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49706 mRNA. Translation: CAA89808.1 .
    AAFI02000112 Genomic DNA. Translation: EAL63189.1 .
    PIRi S72439.
    RefSeqi XP_636697.1. XM_631605.1.

    3D structure databases

    ProteinModelPortali Q94465.
    SMRi Q94465. Positions 48-230.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0191399.

    Proteomic databases

    PRIDEi Q94465.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0191399 ; DDB0191399 ; DDB_G0288481 .
    GeneIDi 8626654.
    KEGGi ddi:DDB_G0288481.

    Organism-specific databases

    dictyBasei DDB_G0288481. gchA.

    Phylogenomic databases

    eggNOGi COG0302.
    KOi K01495.
    OMAi VYSHCEH.
    PhylomeDBi Q94465.

    Enzyme and pathway databases

    UniPathwayi UPA00848 ; UER00151 .
    Reactomei REACT_212091. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.

    Miscellaneous databases

    PROi Q94465.

    Family and domain databases

    HAMAPi MF_00223. FolE.
    InterProi IPR001474. GTP_CycHdrlase_I.
    IPR018234. GTP_CycHdrlase_I_CS.
    IPR020602. GTP_CycHdrlase_I_dom.
    [Graphical view ]
    PANTHERi PTHR11109. PTHR11109. 1 hit.
    Pfami PF01227. GTP_cyclohydroI. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00063. folE. 1 hit.
    PROSITEi PS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
    PS00860. GTP_CYCLOHYDROL_1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a cDNA coding for GTP cyclohydrolase I from Dictyostelium discoideum."
      Witter K., Cahill D.J., Werner T., Ziegler I., Roedl W., Bacher A., Guetlich M.
      Biochem. J. 319:27-32(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: AX2.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
      Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
      Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: AX2.

    Entry informationi

    Entry nameiGCH1_DICDI
    AccessioniPrimary (citable) accession number: Q94465
    Secondary accession number(s): Q54IV6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 92 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3