Q94465 (GCH1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTP cyclohydrolase 1
EC=3.5.4.16

Alternative name(s):
GTP cyclohydrolase I
Short name=GTP-CH-I

Gene names

Name:	gchA
Synonyms:	folE, gtoC
ORF Names:	DDB_G0288481

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate.
Pathway	Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.
Subunit structure	Toroid-shaped homodecamer, composed of two pentamers of five dimers By similarity.
Sequence similarities	Belongs to the GTP cyclohydrolase I family.

Ontologies

Keywords
Biological process	Tetrahydrobiopterin biosynthesis
Ligand	GTP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Hydrolase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	7,8-dihydroneopterin 3'-triphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of G-protein coupled receptor protein signaling pathway Inferred from direct assay PubMed 8619862. Source: dictyBase tetrahydrobiopterin biosynthetic process Inferred from direct assay PubMed 16527408PubMed 8660315Ref.1. Source: dictyBase tetrahydrofolate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	membrane Inferred from direct assay PubMed 8660315. Source: dictyBase phagocytic vesicle Inferred from direct assay Ref.3. Source: dictyBase
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP cyclohydrolase I activity Inferred from direct assay PubMed 16527408PubMed 8660315Ref.1. Source: dictyBase metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 232	232	GTP cyclohydrolase 1		PRO_0000119481
Sites
	Metal binding	121	1	Zinc By similarity
	Metal binding	124	1	Zinc By similarity
	Metal binding	192	1	Zinc By similarity
Experimental info
	Sequence conflict	187	1	E → D in CAA89808. Ref.1
	Sequence conflict	206	1	V → A in CAA89808. Ref.1
	Sequence conflict	216	1	K → E in CAA89808. Ref.1
	Sequence conflict	228 – 232	5	IKSNK → FNSTN in CAA89808. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q94465 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: D36603D793EE5EA2	FASTA	232	26,277
	10 20 30 40 50 60 MSDNLKSYQD NHIENEDEEI YERSNGKGKE LVDFGKKREP LIHNHEVLNT MQSSVKTLLS 70 80 90 100 110 120 SLGEDPDREG LLKTPLRMSK ALLFFTQGYE QSVDEVIGEA IFNENHHEMV VVRDIDIFSL 130 140 150 160 170 180 CEHHMVPFHG KCHIGYIPDQ KVLGLSKLAR VAEIFARRLQ VQERLTRQIA QAIQAHLNPM 190 200 210 220 230 GVAVVIEASH MCMVMRGVQK PGASTVTSSV CGIFEKDSRT RAEFFSLIKS NK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a cDNA coding for GTP cyclohydrolase I from Dictyostelium discoideum." Witter K., Cahill D.J., Werner T., Ziegler I., Roedl W., Bacher A., Guetlich M. Biochem. J. 319:27-32(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: AX2.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: AX2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49706 mRNA. Translation: CAA89808.1. AAFI02000112 Genomic DNA. Translation: EAL63189.1.
PIR	S72439.
RefSeq	XP_636697.1. XM_631605.1.
3D structure databases
ProteinModelPortal	Q94465.
SMR	Q94465. Positions 48-230.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0191399.
Proteomic databases
PRIDE	Q94465.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0191399; DDB0191399; DDB_G0288481.
GeneID	8626654.
KEGG	ddi:DDB_G0288481.
Organism-specific databases
dictyBase	DDB_G0288481. gchA.
Phylogenomic databases
eggNOG	COG0302.
KO	K01495.
OMA	LYSVCEH.
ProtClustDB	PTZ00484.
Enzyme and pathway databases
UniPathway	UPA00848; UER00151.
Family and domain databases
InterPro	IPR001474. GTP_CycHdrlase_I. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. IPR018234. GTP_CycHdrlase_I_CS. [Graphical view]
PANTHER	PTHR11109. PTHR11109. 1 hit.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
TIGRFAMs	TIGR00063. folE. 1 hit.
PROSITE	PS00859. GTP_CYCLOHYDROL_1_1. 1 hit. PS00860. GTP_CYCLOHYDROL_1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCH1_DICDI

Accession

Primary (citable) accession number: Q94465
Secondary accession number(s): Q54IV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)