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Protein

GTP cyclohydrolase 1

Gene

gchA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate.

Pathwayi: 7,8-dihydroneopterin triphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 7,8-dihydroneopterin triphosphate from GTP.
Proteins known to be involved in this subpathway in this organism are:
  1. GTP cyclohydrolase 1 (gchA)
This subpathway is part of the pathway 7,8-dihydroneopterin triphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydroneopterin triphosphate from GTP, the pathway 7,8-dihydroneopterin triphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi121ZincBy similarity1
Metal bindingi124ZincBy similarity1
Metal bindingi192ZincBy similarity1

GO - Molecular functioni

  • GTP binding Source: GO_Central
  • GTP cyclohydrolase I activity Source: dictyBase
  • zinc ion binding Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Tetrahydrobiopterin biosynthesis

Keywords - Ligandi

GTP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DDI-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
UniPathwayiUPA00848; UER00151.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP cyclohydrolase 1 (EC:3.5.4.16)
Alternative name(s):
GTP cyclohydrolase I
Short name:
GTP-CH-I
Gene namesi
Name:gchA
Synonyms:folE, gtoC
ORF Names:DDB_G0288481
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0288481. gchA.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: dictyBase
  • membrane Source: dictyBase
  • phagocytic vesicle Source: dictyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001194811 – 232GTP cyclohydrolase 1Add BLAST232

Proteomic databases

PaxDbiQ94465.

Interactioni

Subunit structurei

Toroid-shaped homodecamer, composed of two pentamers of five dimers.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0191399.

Structurei

3D structure databases

ProteinModelPortaliQ94465.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GTP cyclohydrolase I family.Curated

Phylogenomic databases

eggNOGiKOG2698. Eukaryota.
COG0302. LUCA.
InParanoidiQ94465.
KOiK01495.
OMAiMGKVHIG.
PhylomeDBiQ94465.

Family and domain databases

HAMAPiMF_00223. FolE. 1 hit.
InterProiIPR001474. GTP_CycHdrlase_I.
IPR018234. GTP_CycHdrlase_I_CS.
IPR020602. GTP_CycHdrlase_I_dom.
[Graphical view]
PANTHERiPTHR11109. PTHR11109. 1 hit.
PfamiPF01227. GTP_cyclohydroI. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00063. folE. 1 hit.
PROSITEiPS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
PS00860. GTP_CYCLOHYDROL_1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q94465-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDNLKSYQD NHIENEDEEI YERSNGKGKE LVDFGKKREP LIHNHEVLNT
60 70 80 90 100
MQSSVKTLLS SLGEDPDREG LLKTPLRMSK ALLFFTQGYE QSVDEVIGEA
110 120 130 140 150
IFNENHHEMV VVRDIDIFSL CEHHMVPFHG KCHIGYIPDQ KVLGLSKLAR
160 170 180 190 200
VAEIFARRLQ VQERLTRQIA QAIQAHLNPM GVAVVIEASH MCMVMRGVQK
210 220 230
PGASTVTSSV CGIFEKDSRT RAEFFSLIKS NK
Length:232
Mass (Da):26,277
Last modified:December 4, 2007 - v2
Checksum:iD36603D793EE5EA2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti187E → D in CAA89808 (PubMed:8870645).Curated1
Sequence conflicti206V → A in CAA89808 (PubMed:8870645).Curated1
Sequence conflicti216K → E in CAA89808 (PubMed:8870645).Curated1
Sequence conflicti228 – 232IKSNK → FNSTN in CAA89808 (PubMed:8870645).Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49706 mRNA. Translation: CAA89808.1.
AAFI02000112 Genomic DNA. Translation: EAL63189.1.
PIRiS72439.
RefSeqiXP_636697.1. XM_631605.1.

Genome annotation databases

EnsemblProtistsiEAL63189; EAL63189; DDB_G0288481.
GeneIDi8626654.
KEGGiddi:DDB_G0288481.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49706 mRNA. Translation: CAA89808.1.
AAFI02000112 Genomic DNA. Translation: EAL63189.1.
PIRiS72439.
RefSeqiXP_636697.1. XM_631605.1.

3D structure databases

ProteinModelPortaliQ94465.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0191399.

Proteomic databases

PaxDbiQ94465.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL63189; EAL63189; DDB_G0288481.
GeneIDi8626654.
KEGGiddi:DDB_G0288481.

Organism-specific databases

dictyBaseiDDB_G0288481. gchA.

Phylogenomic databases

eggNOGiKOG2698. Eukaryota.
COG0302. LUCA.
InParanoidiQ94465.
KOiK01495.
OMAiMGKVHIG.
PhylomeDBiQ94465.

Enzyme and pathway databases

UniPathwayiUPA00848; UER00151.
ReactomeiR-DDI-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.

Miscellaneous databases

PROiQ94465.

Family and domain databases

HAMAPiMF_00223. FolE. 1 hit.
InterProiIPR001474. GTP_CycHdrlase_I.
IPR018234. GTP_CycHdrlase_I_CS.
IPR020602. GTP_CycHdrlase_I_dom.
[Graphical view]
PANTHERiPTHR11109. PTHR11109. 1 hit.
PfamiPF01227. GTP_cyclohydroI. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00063. folE. 1 hit.
PROSITEiPS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
PS00860. GTP_CYCLOHYDROL_1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGCH1_DICDI
AccessioniPrimary (citable) accession number: Q94465
Secondary accession number(s): Q54IV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: December 4, 2007
Last modified: November 2, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.