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Q94464 (DYNA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dynamin-A
Gene names
Name:dymA
ORF Names:DDB_G0277849
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length853 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Function in membrane trafficking processes along the endo-lysosomal pathway. Ref.1

Subcellular location

Cytoplasm. Note: Found in association with endosomal and postlysosomal vacuoles. Ref.1

Developmental stage

Present during all stages of development. Ref.1

Sequence similarities

Belongs to the dynamin family.

Contains 1 GED domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandGTP-binding
Nucleotide-binding
   Molecular functionHydrolase
Motor protein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processGTP catabolic process

Inferred from direct assay PubMed 11823417. Source: GOC

actin filament organization

Inferred from mutant phenotype PubMed 23679940. Source: dictyBase

cytokinetic cell separation

Inferred from mutant phenotype PubMed 23679940Ref.1. Source: dictyBase

endosome organization

Inferred from mutant phenotype Ref.1. Source: dictyBase

establishment or maintenance of cell polarity

Inferred from mutant phenotype Ref.1. Source: dictyBase

intracellular distribution of mitochondria

Inferred from mutant phenotype PubMed 22980139. Source: dictyBase

mitochondrion organization

Inferred from mutant phenotype Ref.1. Source: dictyBase

nucleus organization

Inferred from mutant phenotype PubMed 23679940Ref.1. Source: dictyBase

phagocytosis

Inferred from mutant phenotype Ref.1. Source: dictyBase

phagosome acidification

Inferred from mutant phenotype PubMed 22008230. Source: dictyBase

pinocytosis

Inferred from mutant phenotype Ref.1. Source: dictyBase

protein processing in phagocytic vesicle

Inferred from mutant phenotype PubMed 22008230. Source: dictyBase

regulation of post-lysosomal vacuole size

Inferred from mutant phenotype PubMed 22008230. Source: dictyBase

sorocarp morphogenesis

Inferred from mutant phenotype Ref.1. Source: dictyBase

   Cellular_componentcleavage furrow

Inferred from direct assay PubMed 23679940. Source: dictyBase

cytoplasm

Inferred from direct assay Ref.1. Source: dictyBase

phagocytic cup base

Inferred from direct assay PubMed 20200225. Source: dictyBase

phagocytic vesicle

Inferred from direct assay Ref.4PubMed 20200225PubMed 22008230. Source: dictyBase

   Molecular_functionGTP binding

Inferred from direct assay PubMed 11823417. Source: dictyBase

GTPase activity

Inferred from direct assay PubMed 11823417. Source: dictyBase

identical protein binding

Inferred from direct assay PubMed 11823417. Source: dictyBase

phospholipid binding

Inferred from direct assay PubMed 11823417. Source: dictyBase

profilin binding

Inferred from physical interaction PubMed 20200225. Source: dictyBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 853853Dynamin-A
PRO_0000312856

Regions

Domain762 – 85392GED
Nucleotide binding32 – 398GTP By similarity
Nucleotide binding138 – 1425GTP By similarity
Nucleotide binding207 – 2104GTP By similarity
Compositional bias510 – 613104Gln-rich
Compositional bias539 – 728190Asn-rich
Compositional bias643 – 72684Ser-rich

Secondary structure

............................................... 853
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q94464 [UniParc].

Last modified June 1, 1998. Version 2.
Checksum: 5812FF0B90CB97BC

FASTA85396,106
        10         20         30         40         50         60 
MDQLIPVINK LQDVFNTLGS DPLDLPQIVV VGSQSSGKSS VLENIVGRDF LPRGSGIVTR 

        70         80         90        100        110        120 
RPLILQLTHL PIADDGSQTQ EWGEFLHKPN DMFYDFSEIR EEIIRDTDRM TGKNKGISAQ 

       130        140        150        160        170        180 
PINLKIYSPH VVNLTLVDLP GITKVPVGDQ PTDIEQQIRR MVMAYIKKQN AIIVAVTPAN 

       190        200        210        220        230        240 
TDLANSDALQ LAKEVDPEGK RTIGVITKLD LMDKGTDAME VLTGRVIPLT LGFIGVINRS 

       250        260        270        280        290        300 
QEDIIAKKSI RESLKSEILY FKNHPIYKSI ANRSGTAYLS KTLNKLLMFH IRDTLPDLKV 

       310        320        330        340        350        360 
KVSKMLSDVQ GELSTYGDPL YDTKNSQGAL LLQIITIFSS NFKDAIDGKL TDLSNNELYG 

       370        380        390        400        410        420 
GARISYIFNE IYSHCVNNID PLEGISLNDI RTTMRNATGP RAALFIPEIS FELLVKKQVV 

       430        440        450        460        470        480 
RLEEPSAQCV EYVYDELQRI VSQLEAKELS RFINLKARVI EVVNNLLQKH KVPTKTMIEH 

       490        500        510        520        530        540 
LIKIETAFIN TSHPDFVGGE GIFESLYKKQ QLQQQNHLQQ LQDQYQQQQQ QQQQQQQQNG 

       550        560        570        580        590        600 
INNNQKGDNG NMNVNQQNMN QQNMNQQNQS TNPFLQQQQQ GQNKYPGGPP AQQQPNQQPN 

       610        620        630        640        650        660 
QLNKGPQNMP PNQSKPSSIP QNGPNNNNNN NNNNNRQDHQ QGSFFSSFFR ASPDPSLGQY 

       670        680        690        700        710        720 
GGANNSNNSN NPTSPINSSS NSGNNYNTFG GQQSSSSSSQ QLQQSSQSQY KTSYNNNNNS 

       730        740        750        760        770        780 
SSNNSSYNRY QDDFYGRGDK LNQVPSIIKA PDDLTSKEKF ETELIRELLI SYFNIVKKNV 

       790        800        810        820        830        840 
KDSVPKSIMH FLVNQSKEHI QNELVAALYK EELFDELLEE SPQISSKRKS CKAMIEILRK 

       850 
ANEIINEIRD FRN 

« Hide

References

« Hide 'large scale' references
[1]"Disruption of a dynamin homologue affects endocytosis, organelle morphology, and cytokinesis in Dictyostelium discoideum."
Wienke D.C., Knetsch M.L., Neuhaus E.M., Reedy M.C., Manstein D.J.
Mol. Biol. Cell 10:225-243(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
Strain: AX2.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"Identification and isolation of Dictyostelium microtubule-associated protein interactors by tandem affinity purification."
Koch K.V., Reinders Y., Ho T.-H., Sickmann A., Graef R.
Eur. J. Cell Biol. 85:1079-1090(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: AX2.
[4]"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: AX2.
[5]"Crystal structure of a dynamin GTPase domain in both nucleotide-free and GDP-bound forms."
Niemann H.H., Knetsch M.L., Scherer A., Manstein D.J., Kull F.J.
EMBO J. 20:5813-5821(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 2-316.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X99669 mRNA. Translation: CAA67983.1.
AAFI02000023 Genomic DNA. Translation: EAL68097.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1JWYX-ray2.30B2-316[»]
1JX2X-ray2.30B2-316[»]
ProteinModelPortalQ94464.
SMRQ94464. Positions 2-307.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0216177.

Proteomic databases

PRIDEQ94464.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0216177; DDB0216177; DDB_G0277849.
KEGGddi:DDB_G0277849.

Organism-specific databases

dictyBaseDDB_G0277849. dymA.

Phylogenomic databases

eggNOGCOG0699.
KOK17065.
OMAIAHIIEM.
PhylomeDBQ94464.
ProtClustDBCLSZ2846834.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR000375. Dynamin_central.
IPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR022812. Dynamin_SF.
IPR003130. GED.
IPR020850. GTPase_effector_domain_GED.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR11566. PTHR11566. 1 hit.
PfamPF01031. Dynamin_M. 1 hit.
PF00350. Dynamin_N. 1 hit.
PF02212. GED. 1 hit.
[Graphical view]
PRINTSPR00195. DYNAMIN.
SMARTSM00053. DYNc. 1 hit.
SM00302. GED. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00410. DYNAMIN. 1 hit.
PS51388. GED. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ94464.

Entry information

Entry nameDYNA_DICDI
AccessionPrimary (citable) accession number: Q94464
Secondary accession number(s): Q54YU0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: June 1, 1998
Last modified: April 16, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase