Q94464 (DYNA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dynamin-A

Gene names

Name:	dymA
ORF Names:	DDB_G0277849

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	853 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Function in membrane trafficking processes along the endo-lysosomal pathway. Ref.1
Subcellular location	Cytoplasm. Note: Found in association with endosomal and postlysosomal vacuoles. Ref.1
Developmental stage	Present during all stages of development. Ref.1
Sequence similarities	Belongs to the dynamin family. Contains 1 GED domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Hydrolase Motor protein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytokinetic cell separation Inferred from mutant phenotype Ref.1. Source: dictyBase endosome organization Inferred from mutant phenotype Ref.1. Source: dictyBase establishment or maintenance of cell polarity Inferred from mutant phenotype Ref.1. Source: dictyBase intracellular distribution of mitochondria Inferred from mutant phenotype PubMed 22980139. Source: dictyBase nucleus organization Inferred from mutant phenotype Ref.1. Source: dictyBase phagocytosis Inferred from mutant phenotype Ref.1. Source: dictyBase phagosome acidification Inferred from mutant phenotype PubMed 22008230. Source: dictyBase pinocytosis Inferred from mutant phenotype Ref.1. Source: dictyBase protein processing in phagocytic vesicle Inferred from mutant phenotype PubMed 22008230. Source: dictyBase regulation of post-lysosomal vacuole size Inferred from mutant phenotype PubMed 22008230. Source: dictyBase sorocarp morphogenesis Inferred from mutant phenotype Ref.1. Source: dictyBase
Cellular_component	phagocytic cup base Inferred from direct assay PubMed 20200225. Source: dictyBase phagocytic vesicle Inferred from direct assay Ref.4PubMed 20200225PubMed 22008230. Source: dictyBase
Molecular_function	GTP binding Inferred from direct assay PubMed 11823417. Source: dictyBase GTPase activity Inferred from direct assay PubMed 11823417. Source: dictyBase identical protein binding Inferred from direct assay PubMed 11823417. Source: dictyBase phospholipid binding Inferred from direct assay PubMed 11823417. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 853

853

Dynamin-A

PRO_0000312856

Regions

Domain

762 – 853

92

GED

Nucleotide binding

32 – 39

8

GTP By similarity

Nucleotide binding

138 – 142

5

GTP By similarity

Nucleotide binding

207 – 210

4

GTP By similarity

Compositional bias

510 – 613

104

Gln-rich

Compositional bias

539 – 728

190

Asn-rich

Compositional bias

643 – 726

84

Ser-rich

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

853

Helix Strand Turn

Details...

Helix

4 – 14

11

Turn

15 – 17

3

Beta strand

18 – 20

3

Beta strand

27 – 32

6

Beta strand

34 – 37

4

Helix

38 – 46

9

Beta strand

63 – 69

7

Beta strand

82 – 88

7

Helix

97 – 106

10

Beta strand

122 – 128

7

Beta strand

133 – 138

6

Helix

155 – 167

13

Beta strand

171 – 181

11

Helix

188 – 195

8

Beta strand

200 – 207

8

Helix

209 – 211

3

Helix

219 – 222

4

Beta strand

225 – 227

3

Beta strand

233 – 235

3

Helix

241 – 244

4

Beta strand

245 – 247

3

Helix

250 – 262

13

Helix

267 – 269

3

Helix

271 – 273

3

Helix

276 – 305

30

Sequences

	Sequence		Length	Mass (Da)
	Q94464 [UniParc]. Last modified June 1, 1998. Version 2. Checksum: 5812FF0B90CB97BC	FASTA	853	96,106
	10 20 30 40 50 60 MDQLIPVINK LQDVFNTLGS DPLDLPQIVV VGSQSSGKSS VLENIVGRDF LPRGSGIVTR 70 80 90 100 110 120 RPLILQLTHL PIADDGSQTQ EWGEFLHKPN DMFYDFSEIR EEIIRDTDRM TGKNKGISAQ 130 140 150 160 170 180 PINLKIYSPH VVNLTLVDLP GITKVPVGDQ PTDIEQQIRR MVMAYIKKQN AIIVAVTPAN 190 200 210 220 230 240 TDLANSDALQ LAKEVDPEGK RTIGVITKLD LMDKGTDAME VLTGRVIPLT LGFIGVINRS 250 260 270 280 290 300 QEDIIAKKSI RESLKSEILY FKNHPIYKSI ANRSGTAYLS KTLNKLLMFH IRDTLPDLKV 310 320 330 340 350 360 KVSKMLSDVQ GELSTYGDPL YDTKNSQGAL LLQIITIFSS NFKDAIDGKL TDLSNNELYG 370 380 390 400 410 420 GARISYIFNE IYSHCVNNID PLEGISLNDI RTTMRNATGP RAALFIPEIS FELLVKKQVV 430 440 450 460 470 480 RLEEPSAQCV EYVYDELQRI VSQLEAKELS RFINLKARVI EVVNNLLQKH KVPTKTMIEH 490 500 510 520 530 540 LIKIETAFIN TSHPDFVGGE GIFESLYKKQ QLQQQNHLQQ LQDQYQQQQQ QQQQQQQQNG 550 560 570 580 590 600 INNNQKGDNG NMNVNQQNMN QQNMNQQNQS TNPFLQQQQQ GQNKYPGGPP AQQQPNQQPN 610 620 630 640 650 660 QLNKGPQNMP PNQSKPSSIP QNGPNNNNNN NNNNNRQDHQ QGSFFSSFFR ASPDPSLGQY 670 680 690 700 710 720 GGANNSNNSN NPTSPINSSS NSGNNYNTFG GQQSSSSSSQ QLQQSSQSQY KTSYNNNNNS 730 740 750 760 770 780 SSNNSSYNRY QDDFYGRGDK LNQVPSIIKA PDDLTSKEKF ETELIRELLI SYFNIVKKNV 790 800 810 820 830 840 KDSVPKSIMH FLVNQSKEHI QNELVAALYK EELFDELLEE SPQISSKRKS CKAMIEILRK 850 ANEIINEIRD FRN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Disruption of a dynamin homologue affects endocytosis, organelle morphology, and cytokinesis in Dictyostelium discoideum." Wienke D.C., Knetsch M.L., Neuhaus E.M., Reedy M.C., Manstein D.J. Mol. Biol. Cell 10:225-243(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE. Strain: AX2.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Identification and isolation of Dictyostelium microtubule-associated protein interactors by tandem affinity purification." Koch K.V., Reinders Y., Ho T.-H., Sickmann A., Graef R. Eur. J. Cell Biol. 85:1079-1090(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: AX2.
[4]	"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: AX2.
[5]	"Crystal structure of a dynamin GTPase domain in both nucleotide-free and GDP-bound forms." Niemann H.H., Knetsch M.L., Scherer A., Manstein D.J., Kull F.J. EMBO J. 20:5813-5821(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 2-316.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X99669 mRNA. Translation: CAA67983.1.
AAFI02000023 Genomic DNA. Translation: EAL68097.1.

RefSeq

XP_642112.1. XM_637020.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JWY	X-ray	2.30	B	2-316	[»]
1JX2	X-ray	2.30	B	2-316	[»]

ProteinModelPortal

Q94464.

SMR

Q94464. Positions 2-307.

ModBase

Search...

Protein-protein interaction databases

STRING

44689.DDB_0216177.

Proteomic databases

PRIDE

Q94464.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblProtists

DDB0216177; DDB0216177; DDB_G0277849.

GeneID

8621323.

KEGG

ddi:DDB_G0277849.

Organism-specific databases

dictyBase

DDB_G0277849. dymA.

Phylogenomic databases

eggNOG

COG0699.

KO

K01528.

OMA

IAHIIEM.

ProtClustDB

CLSZ2846834.

Family and domain databases

InterPro

IPR022812. Dynamin.
IPR000375. Dynamin_central.
IPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR003130. GED.
IPR020850. GTPase_effector_domain_GED.
[Graphical view]

PANTHER

PTHR11566. PTHR11566. 1 hit.

Pfam

PF01031. Dynamin_M. 1 hit.
PF00350. Dynamin_N. 1 hit.
PF02212. GED. 1 hit.
[Graphical view]

PRINTS

PR00195. DYNAMIN.

SMART

SM00053. DYNc. 1 hit.
SM00302. GED. 1 hit.
[Graphical view]

PROSITE

PS00410. DYNAMIN. 1 hit.
PS51388. GED. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q94464.

Entry information

Entry name

DYNA_DICDI

Accession

Primary (citable) accession number: Q94464
Secondary accession number(s): Q54YU0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 4, 2007
Last sequence update:	June 1, 1998
Last modified:	May 1, 2013
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)