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Q94464

- DYNA_DICDI

UniProt

Q94464 - DYNA_DICDI

Protein

Dynamin-A

Gene

dymA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 2 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Function in membrane trafficking processes along the endo-lysosomal pathway.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi32 – 398GTPBy similarity
    Nucleotide bindingi138 – 1425GTPBy similarity
    Nucleotide bindingi207 – 2104GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: dictyBase
    2. GTP binding Source: dictyBase
    3. identical protein binding Source: dictyBase
    4. phospholipid binding Source: dictyBase
    5. profilin binding Source: dictyBase
    6. protein binding Source: dictyBase

    GO - Biological processi

    1. actin filament organization Source: dictyBase
    2. cytokinetic cell separation Source: dictyBase
    3. endosome organization Source: dictyBase
    4. establishment or maintenance of cell polarity Source: dictyBase
    5. GTP catabolic process Source: GOC
    6. intracellular distribution of mitochondria Source: dictyBase
    7. mitochondrion organization Source: dictyBase
    8. nucleus organization Source: dictyBase
    9. phagocytosis Source: dictyBase
    10. phagosome acidification Source: dictyBase
    11. pinocytosis Source: dictyBase
    12. protein processing in phagocytic vesicle Source: dictyBase
    13. regulation of post-lysosomal vacuole size Source: dictyBase
    14. sorocarp morphogenesis Source: dictyBase

    Keywords - Molecular functioni

    Hydrolase, Motor protein

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_204078. Apoptotic execution phase.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dynamin-A
    Gene namesi
    Name:dymA
    ORF Names:DDB_G0277849
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0277849. dymA.

    Subcellular locationi

    Cytoplasm 1 Publication
    Note: Found in association with endosomal and postlysosomal vacuoles.

    GO - Cellular componenti

    1. cleavage furrow Source: dictyBase
    2. cytoplasm Source: dictyBase
    3. phagocytic cup base Source: dictyBase
    4. phagocytic vesicle Source: dictyBase

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 853853Dynamin-APRO_0000312856Add
    BLAST

    Proteomic databases

    PRIDEiQ94464.

    Expressioni

    Developmental stagei

    Present during all stages of development.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0216177.

    Structurei

    Secondary structure

    1
    853
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 1411
    Turni15 – 173
    Beta strandi18 – 203
    Beta strandi27 – 326
    Beta strandi34 – 374
    Helixi38 – 469
    Beta strandi63 – 697
    Beta strandi82 – 887
    Helixi97 – 10610
    Beta strandi122 – 1287
    Beta strandi133 – 1386
    Helixi155 – 16713
    Beta strandi171 – 18111
    Helixi188 – 1958
    Beta strandi200 – 2078
    Helixi209 – 2113
    Helixi219 – 2224
    Beta strandi225 – 2273
    Beta strandi233 – 2353
    Helixi241 – 2444
    Beta strandi245 – 2473
    Helixi250 – 26213
    Helixi267 – 2693
    Helixi271 – 2733
    Helixi276 – 30530

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1JWYX-ray2.30B2-316[»]
    1JX2X-ray2.30B2-316[»]
    ProteinModelPortaliQ94464.
    SMRiQ94464. Positions 2-307.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ94464.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini22 – 296275Dynamin-type GAdd
    BLAST
    Domaini762 – 85392GEDPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi510 – 613104Gln-richAdd
    BLAST
    Compositional biasi539 – 728190Asn-richAdd
    BLAST
    Compositional biasi643 – 72684Ser-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 GED domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0699.
    KOiK17065.
    OMAiCKAMIEI.
    PhylomeDBiQ94464.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR000375. Dynamin_central.
    IPR001401. Dynamin_GTPase.
    IPR019762. Dynamin_GTPase_CS.
    IPR022812. Dynamin_SF.
    IPR003130. GED.
    IPR020850. GTPase_effector_domain_GED.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR11566. PTHR11566. 1 hit.
    PfamiPF01031. Dynamin_M. 1 hit.
    PF00350. Dynamin_N. 1 hit.
    PF02212. GED. 1 hit.
    [Graphical view]
    PRINTSiPR00195. DYNAMIN.
    SMARTiSM00053. DYNc. 1 hit.
    SM00302. GED. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00410. G_DYNAMIN_1. 1 hit.
    PS51718. G_DYNAMIN_2. 1 hit.
    PS51388. GED. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q94464-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDQLIPVINK LQDVFNTLGS DPLDLPQIVV VGSQSSGKSS VLENIVGRDF    50
    LPRGSGIVTR RPLILQLTHL PIADDGSQTQ EWGEFLHKPN DMFYDFSEIR 100
    EEIIRDTDRM TGKNKGISAQ PINLKIYSPH VVNLTLVDLP GITKVPVGDQ 150
    PTDIEQQIRR MVMAYIKKQN AIIVAVTPAN TDLANSDALQ LAKEVDPEGK 200
    RTIGVITKLD LMDKGTDAME VLTGRVIPLT LGFIGVINRS QEDIIAKKSI 250
    RESLKSEILY FKNHPIYKSI ANRSGTAYLS KTLNKLLMFH IRDTLPDLKV 300
    KVSKMLSDVQ GELSTYGDPL YDTKNSQGAL LLQIITIFSS NFKDAIDGKL 350
    TDLSNNELYG GARISYIFNE IYSHCVNNID PLEGISLNDI RTTMRNATGP 400
    RAALFIPEIS FELLVKKQVV RLEEPSAQCV EYVYDELQRI VSQLEAKELS 450
    RFINLKARVI EVVNNLLQKH KVPTKTMIEH LIKIETAFIN TSHPDFVGGE 500
    GIFESLYKKQ QLQQQNHLQQ LQDQYQQQQQ QQQQQQQQNG INNNQKGDNG 550
    NMNVNQQNMN QQNMNQQNQS TNPFLQQQQQ GQNKYPGGPP AQQQPNQQPN 600
    QLNKGPQNMP PNQSKPSSIP QNGPNNNNNN NNNNNRQDHQ QGSFFSSFFR 650
    ASPDPSLGQY GGANNSNNSN NPTSPINSSS NSGNNYNTFG GQQSSSSSSQ 700
    QLQQSSQSQY KTSYNNNNNS SSNNSSYNRY QDDFYGRGDK LNQVPSIIKA 750
    PDDLTSKEKF ETELIRELLI SYFNIVKKNV KDSVPKSIMH FLVNQSKEHI 800
    QNELVAALYK EELFDELLEE SPQISSKRKS CKAMIEILRK ANEIINEIRD 850
    FRN 853
    Length:853
    Mass (Da):96,106
    Last modified:June 1, 1998 - v2
    Checksum:i5812FF0B90CB97BC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X99669 mRNA. Translation: CAA67983.1.
    AAFI02000023 Genomic DNA. Translation: EAL68097.1.
    RefSeqiXP_642112.1. XM_637020.1.

    Genome annotation databases

    EnsemblProtistsiDDB0216177; DDB0216177; DDB_G0277849.
    GeneIDi8621323.
    KEGGiddi:DDB_G0277849.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X99669 mRNA. Translation: CAA67983.1 .
    AAFI02000023 Genomic DNA. Translation: EAL68097.1 .
    RefSeqi XP_642112.1. XM_637020.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1JWY X-ray 2.30 B 2-316 [» ]
    1JX2 X-ray 2.30 B 2-316 [» ]
    ProteinModelPortali Q94464.
    SMRi Q94464. Positions 2-307.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0216177.

    Proteomic databases

    PRIDEi Q94464.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0216177 ; DDB0216177 ; DDB_G0277849 .
    GeneIDi 8621323.
    KEGGi ddi:DDB_G0277849.

    Organism-specific databases

    dictyBasei DDB_G0277849. dymA.

    Phylogenomic databases

    eggNOGi COG0699.
    KOi K17065.
    OMAi CKAMIEI.
    PhylomeDBi Q94464.

    Enzyme and pathway databases

    Reactomei REACT_204078. Apoptotic execution phase.

    Miscellaneous databases

    EvolutionaryTracei Q94464.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR000375. Dynamin_central.
    IPR001401. Dynamin_GTPase.
    IPR019762. Dynamin_GTPase_CS.
    IPR022812. Dynamin_SF.
    IPR003130. GED.
    IPR020850. GTPase_effector_domain_GED.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR11566. PTHR11566. 1 hit.
    Pfami PF01031. Dynamin_M. 1 hit.
    PF00350. Dynamin_N. 1 hit.
    PF02212. GED. 1 hit.
    [Graphical view ]
    PRINTSi PR00195. DYNAMIN.
    SMARTi SM00053. DYNc. 1 hit.
    SM00302. GED. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00410. G_DYNAMIN_1. 1 hit.
    PS51718. G_DYNAMIN_2. 1 hit.
    PS51388. GED. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Disruption of a dynamin homologue affects endocytosis, organelle morphology, and cytokinesis in Dictyostelium discoideum."
      Wienke D.C., Knetsch M.L., Neuhaus E.M., Reedy M.C., Manstein D.J.
      Mol. Biol. Cell 10:225-243(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
      Strain: AX2.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "Identification and isolation of Dictyostelium microtubule-associated protein interactors by tandem affinity purification."
      Koch K.V., Reinders Y., Ho T.-H., Sickmann A., Graef R.
      Eur. J. Cell Biol. 85:1079-1090(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: AX2.
    4. "Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
      Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
      Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: AX2.
    5. "Crystal structure of a dynamin GTPase domain in both nucleotide-free and GDP-bound forms."
      Niemann H.H., Knetsch M.L., Scherer A., Manstein D.J., Kull F.J.
      EMBO J. 20:5813-5821(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 2-316.

    Entry informationi

    Entry nameiDYNA_DICDI
    AccessioniPrimary (citable) accession number: Q94464
    Secondary accession number(s): Q54YU0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 4, 2007
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 94 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3