Q94425 (SYIM_CIOIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Probable isoleucine--tRNA ligase, mitochondrial EC=6.1.1.5 Alternative name(s): Isoleucyl-tRNA synthetase Short name=IleRS
Organism	Ciona intestinalis (Transparent sea squirt) (Ascidia intestinalis) [Reference proteome]
Taxonomic identifier	7719 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Tunicata › Ascidiacea › Enterogona › Phlebobranchia › Cionidae › Ciona

Protein attributes

Sequence length	413 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 413	›413	Probable isoleucine--tRNA ligase, mitochondrial		PRO_0000098600
Regions
	Motif	298 – 302	5	"KMSKS" region
Sites
	Binding site	301	1	ATP By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q94425 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 69B098FD71C100CE	FASTA	413	46,895
	10 20 30 40 50 60 GMICRHPIYL ERTVRMIGSD IANTSKGTGV VHIAPAHGMD DFGIGQKHNL STESSVNSDG 70 80 90 100 110 120 EFMANIAPNL EGKFIFTSGN EAVVEMLKEE MNLFHEHDYK HSYPYDWRTK QPIFVHTSNQ 130 140 150 160 170 180 WFVDTAMLAN KAEAAISGIT TYPDNGISSM LNRLQLRSYW CISRQRAWGL PIPVFYHKES 190 200 210 220 230 240 GELLLNESTI QHLVSLVLKH GADIWWEGTE LLPTQYNADE YERGRDILDI WFDSGVSWDC 250 260 270 280 290 300 VLRDSKNRTT QADFYIEGKD QYGGWFQSSL LTSVGLQGVA PYKNLMVHGF VLDEKGQKMS 310 320 330 340 350 360 KSIGNVVDPM VLTNGGKNPQ RDPAYGADAL RWWVAESNVH QDVHISTTII KSAAESVQKI 370 380 390 400 410 RNSVRFMLGN LNEFDKMKIV PTPAMLILDL RYRFEDKKLW YVTSMNYPLN LSV

References

[1]	Bird A.P., Clark V., Jones S.J.M., Leitgeb S., Lennard N., Tweedie S. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z80904 Genomic DNA. Translation: CAB02584.1.
PIR	T31663.
3D structure databases
ProteinModelPortal	Q94425.
ModBase	Search...
Protein-protein interaction databases
STRING	7719.ENSCINP00000017328.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	COG0060.
Family and domain databases
Gene3D	3.40.50.620. 1 hit. 3.90.740.10. 1 hit.
InterPro	IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-ligase. IPR023585. Ile-tRNA-ligase_type1. IPR014729. Rossmann-like_a/b/a_fold. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
PANTHER	PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfam	PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF50677. ValRS_IleRS_edit. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYIM_CIOIN

Accession

Primary (citable) accession number: Q94425

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program