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Q94425 (SYIM_CIOIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable isoleucine--tRNA ligase, mitochondrial

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
OrganismCiona intestinalis (Transparent sea squirt) (Ascidia intestinalis) [Reference proteome]
Taxonomic identifier7719 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataTunicataAscidiaceaEnterogonaPhlebobranchiaCionidaeCiona

Protein attributes

Sequence length413 AA.
Sequence statusFragment.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Subcellular location

Mitochondrion matrix By similarity.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentMitochondrion
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processisoleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentmitochondrial matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

isoleucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 413›413Probable isoleucine--tRNA ligase, mitochondrial
PRO_0000098600

Regions

Motif298 – 3025"KMSKS" region

Sites

Binding site3011ATP By similarity

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
Q94425 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 69B098FD71C100CE

FASTA41346,895
        10         20         30         40         50         60 
GMICRHPIYL ERTVRMIGSD IANTSKGTGV VHIAPAHGMD DFGIGQKHNL STESSVNSDG 

        70         80         90        100        110        120 
EFMANIAPNL EGKFIFTSGN EAVVEMLKEE MNLFHEHDYK HSYPYDWRTK QPIFVHTSNQ 

       130        140        150        160        170        180 
WFVDTAMLAN KAEAAISGIT TYPDNGISSM LNRLQLRSYW CISRQRAWGL PIPVFYHKES 

       190        200        210        220        230        240 
GELLLNESTI QHLVSLVLKH GADIWWEGTE LLPTQYNADE YERGRDILDI WFDSGVSWDC 

       250        260        270        280        290        300 
VLRDSKNRTT QADFYIEGKD QYGGWFQSSL LTSVGLQGVA PYKNLMVHGF VLDEKGQKMS 

       310        320        330        340        350        360 
KSIGNVVDPM VLTNGGKNPQ RDPAYGADAL RWWVAESNVH QDVHISTTII KSAAESVQKI 

       370        380        390        400        410 
RNSVRFMLGN LNEFDKMKIV PTPAMLILDL RYRFEDKKLW YVTSMNYPLN LSV 

« Hide

References

[1]Bird A.P., Clark V., Jones S.J.M., Leitgeb S., Lennard N., Tweedie S.
Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z80904 Genomic DNA. Translation: CAB02584.1.
PIRT31663.

3D structure databases

ProteinModelPortalQ94425.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7719.ENSCINP00000017328.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG0060.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 1 hit.
3.90.740.10. 1 hit.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF50677. SSF50677. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYIM_CIOIN
AccessionPrimary (citable) accession number: Q94425
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries