Q94420 (MEL26_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein maternal effect lethal 26

Gene names

Name:	mel-26
ORF Names:	ZK858.4

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Controls degradation of mei-1. Ref.3 Ref.4
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with cul-3. Seems to be a component of a E3 ubiquitin-protein ligase complex containing cul-3. Ref.3 Ref.4
Sequence similarities	Contains 1 BTB (POZ) domain. Contains 1 MATH domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	body morphogenesis Inferred from mutant phenotype. Source: WormBase embryo development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase hatching Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase
Cellular component	cytoplasm Inferred from direct assay. Source: WormBase membrane Inferred from direct assay. Source: WormBase
Molecular function	identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		3	EBI-320790,EBI-320790
mei-1	P34808	4	EBI-320790,EBI-323248
R02F2.5	Q21648	3	EBI-320790,EBI-314179
smo-1	P55853	3	EBI-320790,EBI-313647
Y105C5A.1	Q9NF71	3	EBI-320790,EBI-332478

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Protein maternal effect lethal 26		PRO_0000312628
Regions
	Domain	41 – 162	122	MATH
	Domain	201 – 269	69	BTB

Sequences

	Sequence		Length	Mass (Da)
	Q94420 [UniParc]. Last modified June 1, 2002. Version 2. Checksum: 3AED944583A155AC	FASTA	395	44,492
	10 20 30 40 50 60 MEPRIDGGVF IGGIGNSGNE MCSNGVPALG VSSQTEIKVE KVQHTWTVKN FSHCYQEYLE 70 80 90 100 110 120 NFVYLQRGDE QLTWSIKIYP KGNGENNKDF VFLCLNRVIN NNVKAGKIGF KSQFKLRTAE 130 140 150 160 170 180 NKDIEMRIHP NPSHSDYVSY IKRDVLFPQI MPRDMIIVNV EIDVAVETIT TTNEPIQFEP 190 200 210 220 230 240 TNSEQQLIED YQRLFSQELL CDFAINVNGK IIRAHKAVLA ARSPVFNAML THQDTDEAKS 250 260 270 280 290 300 SMMYINDMDY DVIYEMVYYI YCGRCNKDIT DMATALLIAA DKYRLEELKS HCEKYLVENI 310 320 330 340 350 360 NIENACSLLI IGDLYSAPKL RKRAVTYILA RPKNVTGTPG WEDILKGHPN LITDIFSQID 370 380 390 RQSSTGATSS VSNLPGVPMD IPGITGNIVP PPSGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic and molecular characterization of the Caenorhabditis elegans gene, mel-26, a postmeiotic negative regulator of mei-1, a meiotic-specific spindle component." Dow M.R., Mains P.E. Genetics 150:119-128(1998) [PubMed: 9725834] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"Targeting of protein ubiquitination by BTB-Cullin 3-Roc1 ubiquitin ligases." Furukawa M., He Y.J., Borchers C., Xiong Y. Nat. Cell Biol. 5:1001-1007(2003) [PubMed: 14528312] [Abstract] Cited for: FUNCTION AS A E3 UBIQUITIN-PROTEIN LIGASE, INTERACTION WITH CUL3.
[4]	"BTB proteins are substrate-specific adaptors in an SCF-like modular ubiquitin ligase containing CUL-3." Xu L., Wei Y., Reboul J., Vaglio P., Shin T.H., Vidal M., Elledge S.J., Harper J.W. Nature 425:316-321(2003) [PubMed: 13679922] [Abstract] Cited for: FUNCTION AS A E3 UBIQUITIN-PROTEIN LIGASE, INTERACTION WITH CUL3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U67737 Genomic DNA. Translation: AAC63596.1. Z79759 Genomic DNA. Translation: CAB02139.2.
PIR	T28056.
RefSeq	NP_492449.2. NM_060048.7.
UniGene	Cel.5086.
3D structure databases
ProteinModelPortal	Q94420.
SMR	Q94420. Positions 38-332.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-27179N.
IntAct	Q94420. 31 interactions.
MINT	MINT-274292.
STRING	Q94420.
Proteomic databases
PRIDE	Q94420.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	ZK858.4; ZK858.4; ZK858.4.
GeneID	172737.
KEGG	cel:ZK858.4.
NMPDR	fig|6239.3.peg.2418.
UCSC	ZK858.4. c. elegans.
Organism-specific databases
CTD	172737.
WormBase	ZK858.4; CE30580; WBGene00003209; mel-26.
Phylogenomic databases
eggNOG	meNOG05246.
GeneTree	EMGT00050000001403.
HOGENOM	HBG377801.
InParanoid	Q94420.
OMA	HPNLITD.
PhylomeDB	Q94420.
Gene expression databases
ArrayExpress	Q94420.
Family and domain databases
InterPro	IPR000210. BTB/POZ-like. IPR011333. BTB/POZ_fold. IPR013069. BTB_POZ. IPR002083. MATH. IPR008974. TRAF-like. IPR013322. TRAF-type. [Graphical view]
Gene3D	G3DSA:3.30.710.10. BTB/POZ_fold. 1 hit. G3DSA:2.60.210.10. TRAF-type. 1 hit.
KO	K03876.
Pfam	PF00651. BTB. 1 hit. PF00917. MATH. 1 hit. [Graphical view]
SMART	SM00225. BTB. 1 hit. SM00061. MATH. 1 hit. [Graphical view]
SUPFAM	SSF54695. BTB/POZ_fold. 1 hit. SSF49599. Traf_like. 1 hit.
PROSITE	PS50097. BTB. 1 hit. PS50144. MATH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	876803.

Entry information

Entry name

MEL26_CAEEL

Accession

Primary (citable) accession number: Q94420
Secondary accession number(s): Q94139

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 4, 2007
Last sequence update:	June 1, 2002
Last modified:	November 16, 2011
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project