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Q94392 (NSF_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vesicle-fusing ATPase

EC=3.6.4.6
Alternative name(s):
N-ethylmaleimide-sensitive fusion protein
Short name=NEM-sensitive fusion protein
Vesicular-fusion protein NSF
Gene names
Name:nsf-1
ORF Names:H15N14.2
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length824 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin By similarity.

Catalytic activity

ATP + H2O = ADP + phosphate.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Subunit structure

Homohexamer By similarity.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the AAA ATPase family.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentCytoplasm
   DomainRepeat
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

nucleoside-triphosphatase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

pas-2Q274883EBI-316816,EBI-318271

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 824824Vesicle-fusing ATPase
PRO_0000084565

Regions

Nucleotide binding582 – 5876ATP By similarity
Nucleotide binding622 – 6298ATP By similarity

Sites

Metal binding6271Magnesium

Sequences

Sequence LengthMass (Da)Tools
Q94392 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: 67232C5115B0B03A

FASTA82491,335
        10         20         30         40         50         60 
MSPVPCLSTC CCFRKIVEYS TMSWFRKSAN DSLLETNHDR IPVAPPREVR APSPRLPPSY 

        70         80         90        100        110        120 
QTSNEKMFRV RKAPSEEHTL ANYAYVNRSD FDDKQIKHVR VNPGPAHHYI FSIRNDGSIK 

       130        140        150        160        170        180 
PGEIAFGVPH RKWAALSLDQ EVRVTPFTFQ QSEYVGSMIL TADFNAKKNV TSEPLNADLM 

       190        200        210        220        230        240 
AREFSIQFGG QAFSKGMQMA FRFEDKEKNK THTLSLVVKS IEGFDIGKAA AAASGASNTD 

       250        260        270        280        290        300 
SSATKPKQIE AGELLPNSVI VFDKEEGSML NLIGKSKGKS AYRSIINPDW DFQQMGIGGL 

       310        320        330        340        350        360 
DTEFSHIFRR AFASRVFPPE FIEQLGMKHV RGILLFGPPG TGKTLMARQI GKMLNAREPK 

       370        380        390        400        410        420 
IVNGPQILDK YVGESESNVR KLFADAEEEW RRCGANSGLH IIIFDEIDAI CKQRGSMAGS 

       430        440        450        460        470        480 
SSVHDTVVNQ LLSKMDGVEQ LNNILVIGMT NRRDMIDEAL LRPGRLEVQM EVSLPDETGR 

       490        500        510        520        530        540 
LQILKIHTAR MREYNKMDPN VDLEDISKRT KNFSGAELEG LVRAAQSSAM NRLVKAGGKA 

       550        560        570        580        590        600 
QADPDAIEKL AINSGDFDYA LENDIKPAFG RSDESLNRFL SRGMIVWGPE VTKILDEGSL 

       610        620        630        640        650        660 
LAATVKNPEN SGFRTVVLAG AAKTGKTSLA AQMAKSSDFP FVKVISPEDT VGFSESAKCM 

       670        680        690        700        710        720 
ALKKAFEDAK RSKLSVLLID NLERLIDYHP VGPRYSNLVI QALLVLLNAP PPAGHRLFVI 

       730        740        750        760        770        780 
ATSSDRMFLR DMGLMDVFGD VIDIPKLTTA GQMMNVIQES NIYSDDQLPM IEQKLASICR 

       790        800        810        820 
GEGFHGVGIK HLLELIESAR QCEADYRVPT LLNMMEGLAL NLYR 

« Hide

References

[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z96100, Z79698 Genomic DNA. Translation: CAB09531.1.
PIRT23096.
RefSeqNP_001076603.1. NM_001083134.3.
UniGeneCel.17352.

3D structure databases

ProteinModelPortalQ94392.
SMRQ94392. Positions 66-815.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid57330. 5 interactions.
DIPDIP-24326N.
IntActQ94392. 4 interactions.
MINTMINT-212260.

Proteomic databases

PaxDbQ94392.
PRIDEQ94392.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaH15N14.2a; H15N14.2a; H15N14.2.
GeneID266842.
KEGGcel:CELE_H15N14.2.
UCSCH15N14.2a. c. elegans.

Organism-specific databases

CTD266842.
WormBaseH15N14.2a; CE19925; WBGene00003818; nsf-1.

Phylogenomic databases

eggNOGCOG0464.
HOGENOMHOG000198544.
InParanoidQ94392.
KOK06027.
OMAVQQAKAP.
PhylomeDBQ94392.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio953236.
PROQ94392.

Entry information

Entry nameNSF_CAEEL
AccessionPrimary (citable) accession number: Q94392
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 30, 2000
Last modified: April 16, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase