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Q94392

- NSF_CAEEL

UniProt

Q94392 - NSF_CAEEL

Protein

Vesicle-fusing ATPase

Gene

nsf-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    Functioni

    Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin By similarity.By similarity

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi627 – 6271Magnesium

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi582 – 5876ATPBy similarity
    Nucleotide bindingi622 – 6298ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. nucleoside-triphosphatase activity Source: InterPro
    4. protein binding Source: IntAct

    GO - Biological processi

    1. protein transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Protein transport, Transport

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vesicle-fusing ATPase (EC:3.6.4.6)
    Alternative name(s):
    N-ethylmaleimide-sensitive fusion protein
    Short name:
    NEM-sensitive fusion protein
    Vesicular-fusion protein NSF
    Gene namesi
    Name:nsf-1
    ORF Names:H15N14.2
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome I

    Organism-specific databases

    WormBaseiH15N14.2a; CE19925; WBGene00003818; nsf-1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 824824Vesicle-fusing ATPasePRO_0000084565Add
    BLAST

    Proteomic databases

    PaxDbiQ94392.
    PRIDEiQ94392.

    Interactioni

    Subunit structurei

    Homohexamer.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    pas-2Q274883EBI-316816,EBI-318271

    Protein-protein interaction databases

    BioGridi57330. 5 interactions.
    DIPiDIP-24326N.
    IntActiQ94392. 4 interactions.
    MINTiMINT-212260.

    Structurei

    3D structure databases

    ProteinModelPortaliQ94392.
    SMRiQ94392. Positions 66-815.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AAA ATPase family.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0464.
    GeneTreeiENSGT00530000064085.
    HOGENOMiHOG000198544.
    InParanoidiQ94392.
    KOiK06027.
    OMAiESARQCE.
    PhylomeDBiQ94392.

    Family and domain databases

    Gene3Di3.10.330.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR009010. Asp_de-COase-like_dom.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR004201. Cdc48_dom2.
    IPR029067. CDC48_domain_2-like.
    IPR003338. CDC4_N-term_subdom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00004. AAA. 2 hits.
    PF02933. CDC48_2. 1 hit.
    PF02359. CDC48_N. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 2 hits.
    SM01073. CDC48_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF54585. SSF54585. 1 hit.
    PROSITEiPS00674. AAA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q94392-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSPVPCLSTC CCFRKIVEYS TMSWFRKSAN DSLLETNHDR IPVAPPREVR    50
    APSPRLPPSY QTSNEKMFRV RKAPSEEHTL ANYAYVNRSD FDDKQIKHVR 100
    VNPGPAHHYI FSIRNDGSIK PGEIAFGVPH RKWAALSLDQ EVRVTPFTFQ 150
    QSEYVGSMIL TADFNAKKNV TSEPLNADLM AREFSIQFGG QAFSKGMQMA 200
    FRFEDKEKNK THTLSLVVKS IEGFDIGKAA AAASGASNTD SSATKPKQIE 250
    AGELLPNSVI VFDKEEGSML NLIGKSKGKS AYRSIINPDW DFQQMGIGGL 300
    DTEFSHIFRR AFASRVFPPE FIEQLGMKHV RGILLFGPPG TGKTLMARQI 350
    GKMLNAREPK IVNGPQILDK YVGESESNVR KLFADAEEEW RRCGANSGLH 400
    IIIFDEIDAI CKQRGSMAGS SSVHDTVVNQ LLSKMDGVEQ LNNILVIGMT 450
    NRRDMIDEAL LRPGRLEVQM EVSLPDETGR LQILKIHTAR MREYNKMDPN 500
    VDLEDISKRT KNFSGAELEG LVRAAQSSAM NRLVKAGGKA QADPDAIEKL 550
    AINSGDFDYA LENDIKPAFG RSDESLNRFL SRGMIVWGPE VTKILDEGSL 600
    LAATVKNPEN SGFRTVVLAG AAKTGKTSLA AQMAKSSDFP FVKVISPEDT 650
    VGFSESAKCM ALKKAFEDAK RSKLSVLLID NLERLIDYHP VGPRYSNLVI 700
    QALLVLLNAP PPAGHRLFVI ATSSDRMFLR DMGLMDVFGD VIDIPKLTTA 750
    GQMMNVIQES NIYSDDQLPM IEQKLASICR GEGFHGVGIK HLLELIESAR 800
    QCEADYRVPT LLNMMEGLAL NLYR 824
    Length:824
    Mass (Da):91,335
    Last modified:May 30, 2000 - v2
    Checksum:i67232C5115B0B03A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z96100, Z79698 Genomic DNA. Translation: CAB09531.1.
    PIRiT23096.
    RefSeqiNP_001076603.1. NM_001083134.3.
    UniGeneiCel.17352.

    Genome annotation databases

    EnsemblMetazoaiH15N14.2a; H15N14.2a; WBGene00003818.
    GeneIDi266842.
    KEGGicel:CELE_H15N14.2.
    UCSCiH15N14.2a. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z96100 , Z79698 Genomic DNA. Translation: CAB09531.1 .
    PIRi T23096.
    RefSeqi NP_001076603.1. NM_001083134.3.
    UniGenei Cel.17352.

    3D structure databases

    ProteinModelPortali Q94392.
    SMRi Q94392. Positions 66-815.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 57330. 5 interactions.
    DIPi DIP-24326N.
    IntActi Q94392. 4 interactions.
    MINTi MINT-212260.

    Proteomic databases

    PaxDbi Q94392.
    PRIDEi Q94392.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai H15N14.2a ; H15N14.2a ; WBGene00003818 .
    GeneIDi 266842.
    KEGGi cel:CELE_H15N14.2.
    UCSCi H15N14.2a. c. elegans.

    Organism-specific databases

    CTDi 266842.
    WormBasei H15N14.2a ; CE19925 ; WBGene00003818 ; nsf-1.

    Phylogenomic databases

    eggNOGi COG0464.
    GeneTreei ENSGT00530000064085.
    HOGENOMi HOG000198544.
    InParanoidi Q94392.
    KOi K06027.
    OMAi ESARQCE.
    PhylomeDBi Q94392.

    Miscellaneous databases

    NextBioi 953236.
    PROi Q94392.

    Family and domain databases

    Gene3Di 3.10.330.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR009010. Asp_de-COase-like_dom.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR004201. Cdc48_dom2.
    IPR029067. CDC48_domain_2-like.
    IPR003338. CDC4_N-term_subdom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00004. AAA. 2 hits.
    PF02933. CDC48_2. 1 hit.
    PF02359. CDC48_N. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 2 hits.
    SM01073. CDC48_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF54585. SSF54585. 1 hit.
    PROSITEi PS00674. AAA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.

    Entry informationi

    Entry nameiNSF_CAEEL
    AccessioniPrimary (citable) accession number: Q94392
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3