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Protein

Vesicle-fusing ATPase

Gene

nsf-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi627MagnesiumBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi582 – 587ATPBy similarity6
Nucleotide bindingi622 – 629ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processProtein transport, Transport
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-204005 COPII-mediated vesicle transport
R-CEL-6807878 COPI-mediated anterograde transport
R-CEL-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-CEL-6811438 Intra-Golgi traffic
R-CEL-6811440 Retrograde transport at the Trans-Golgi-Network

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-fusing ATPase (EC:3.6.4.6)
Alternative name(s):
N-ethylmaleimide-sensitive fusion protein
Short name:
NEM-sensitive fusion protein
Vesicular-fusion protein NSF
Gene namesi
Name:nsf-1
ORF Names:H15N14.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi

Organism-specific databases

WormBaseiH15N14.2a ; CE19925 ; WBGene00003818 ; nsf-1

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000845651 – 824Vesicle-fusing ATPaseAdd BLAST824

Proteomic databases

EPDiQ94392
PaxDbiQ94392
PeptideAtlasiQ94392
PRIDEiQ94392

Expressioni

Gene expression databases

BgeeiWBGene00003818
ExpressionAtlasiQ94392 baseline and differential

Interactioni

Subunit structurei

Homohexamer.By similarity

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi57330, 4 interactors
DIPiDIP-24326N
IntActiQ94392, 4 interactors
STRINGi6239.H15N14.2a

Structurei

3D structure databases

ProteinModelPortaliQ94392
SMRiQ94392
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0741 Eukaryota
COG0464 LUCA
GeneTreeiENSGT00530000064085
HOGENOMiHOG000198544
InParanoidiQ94392
KOiK06027
OMAiIDWVPIG
OrthoDBiEOG091G02LU
PhylomeDBiQ94392

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR009010 Asp_de-COase-like_dom_sf
IPR003959 ATPase_AAA_core
IPR003960 ATPase_AAA_CS
IPR004201 Cdc48_dom2
IPR029067 CDC48_domain_2-like_sf
IPR003338 CDC4_N-term_subdom
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00004 AAA, 2 hits
PF02933 CDC48_2, 1 hit
PF02359 CDC48_N, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SM01073 CDC48_N, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
SSF52540 SSF52540, 2 hits
SSF54585 SSF54585, 1 hit
PROSITEiView protein in PROSITE
PS00674 AAA, 1 hit

Sequencei

Sequence statusi: Complete.

Q94392-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPVPCLSTC CCFRKIVEYS TMSWFRKSAN DSLLETNHDR IPVAPPREVR
60 70 80 90 100
APSPRLPPSY QTSNEKMFRV RKAPSEEHTL ANYAYVNRSD FDDKQIKHVR
110 120 130 140 150
VNPGPAHHYI FSIRNDGSIK PGEIAFGVPH RKWAALSLDQ EVRVTPFTFQ
160 170 180 190 200
QSEYVGSMIL TADFNAKKNV TSEPLNADLM AREFSIQFGG QAFSKGMQMA
210 220 230 240 250
FRFEDKEKNK THTLSLVVKS IEGFDIGKAA AAASGASNTD SSATKPKQIE
260 270 280 290 300
AGELLPNSVI VFDKEEGSML NLIGKSKGKS AYRSIINPDW DFQQMGIGGL
310 320 330 340 350
DTEFSHIFRR AFASRVFPPE FIEQLGMKHV RGILLFGPPG TGKTLMARQI
360 370 380 390 400
GKMLNAREPK IVNGPQILDK YVGESESNVR KLFADAEEEW RRCGANSGLH
410 420 430 440 450
IIIFDEIDAI CKQRGSMAGS SSVHDTVVNQ LLSKMDGVEQ LNNILVIGMT
460 470 480 490 500
NRRDMIDEAL LRPGRLEVQM EVSLPDETGR LQILKIHTAR MREYNKMDPN
510 520 530 540 550
VDLEDISKRT KNFSGAELEG LVRAAQSSAM NRLVKAGGKA QADPDAIEKL
560 570 580 590 600
AINSGDFDYA LENDIKPAFG RSDESLNRFL SRGMIVWGPE VTKILDEGSL
610 620 630 640 650
LAATVKNPEN SGFRTVVLAG AAKTGKTSLA AQMAKSSDFP FVKVISPEDT
660 670 680 690 700
VGFSESAKCM ALKKAFEDAK RSKLSVLLID NLERLIDYHP VGPRYSNLVI
710 720 730 740 750
QALLVLLNAP PPAGHRLFVI ATSSDRMFLR DMGLMDVFGD VIDIPKLTTA
760 770 780 790 800
GQMMNVIQES NIYSDDQLPM IEQKLASICR GEGFHGVGIK HLLELIESAR
810 820
QCEADYRVPT LLNMMEGLAL NLYR
Length:824
Mass (Da):91,335
Last modified:May 30, 2000 - v2
Checksum:i67232C5115B0B03A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z96100, Z79698 Genomic DNA Translation: CAB09531.1
PIRiT23096
RefSeqiNP_001076603.1, NM_001083134.3
UniGeneiCel.17352

Genome annotation databases

EnsemblMetazoaiH15N14.2a; H15N14.2a; WBGene00003818
GeneIDi266842
KEGGicel:CELE_H15N14.2
UCSCiH15N14.2a c. elegans

Similar proteinsi

Entry informationi

Entry nameiNSF_CAEEL
AccessioniPrimary (citable) accession number: Q94392
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 30, 2000
Last modified: May 23, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

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