Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Vesicle-fusing ATPase

Gene

nsf-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi627 – 6271Magnesium

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi582 – 5876ATPBy similarity
Nucleotide bindingi622 – 6298ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-fusing ATPase (EC:3.6.4.6)
Alternative name(s):
N-ethylmaleimide-sensitive fusion protein
Short name:
NEM-sensitive fusion protein
Vesicular-fusion protein NSF
Gene namesi
Name:nsf-1
ORF Names:H15N14.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiH15N14.2a; CE19925; WBGene00003818; nsf-1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 824824Vesicle-fusing ATPasePRO_0000084565Add
BLAST

Proteomic databases

PaxDbiQ94392.
PRIDEiQ94392.

Expressioni

Gene expression databases

ExpressionAtlasiQ94392. baseline.

Interactioni

Subunit structurei

Homohexamer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
pas-2Q274883EBI-316816,EBI-318271

Protein-protein interaction databases

BioGridi57330. 5 interactions.
DIPiDIP-24326N.
IntActiQ94392. 4 interactions.
MINTiMINT-212260.

Structurei

3D structure databases

ProteinModelPortaliQ94392.
SMRiQ94392. Positions 66-815.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0464.
GeneTreeiENSGT00530000064085.
HOGENOMiHOG000198544.
InParanoidiQ94392.
KOiK06027.
OMAiHDEDPAN.
PhylomeDBiQ94392.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q94392-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPVPCLSTC CCFRKIVEYS TMSWFRKSAN DSLLETNHDR IPVAPPREVR
60 70 80 90 100
APSPRLPPSY QTSNEKMFRV RKAPSEEHTL ANYAYVNRSD FDDKQIKHVR
110 120 130 140 150
VNPGPAHHYI FSIRNDGSIK PGEIAFGVPH RKWAALSLDQ EVRVTPFTFQ
160 170 180 190 200
QSEYVGSMIL TADFNAKKNV TSEPLNADLM AREFSIQFGG QAFSKGMQMA
210 220 230 240 250
FRFEDKEKNK THTLSLVVKS IEGFDIGKAA AAASGASNTD SSATKPKQIE
260 270 280 290 300
AGELLPNSVI VFDKEEGSML NLIGKSKGKS AYRSIINPDW DFQQMGIGGL
310 320 330 340 350
DTEFSHIFRR AFASRVFPPE FIEQLGMKHV RGILLFGPPG TGKTLMARQI
360 370 380 390 400
GKMLNAREPK IVNGPQILDK YVGESESNVR KLFADAEEEW RRCGANSGLH
410 420 430 440 450
IIIFDEIDAI CKQRGSMAGS SSVHDTVVNQ LLSKMDGVEQ LNNILVIGMT
460 470 480 490 500
NRRDMIDEAL LRPGRLEVQM EVSLPDETGR LQILKIHTAR MREYNKMDPN
510 520 530 540 550
VDLEDISKRT KNFSGAELEG LVRAAQSSAM NRLVKAGGKA QADPDAIEKL
560 570 580 590 600
AINSGDFDYA LENDIKPAFG RSDESLNRFL SRGMIVWGPE VTKILDEGSL
610 620 630 640 650
LAATVKNPEN SGFRTVVLAG AAKTGKTSLA AQMAKSSDFP FVKVISPEDT
660 670 680 690 700
VGFSESAKCM ALKKAFEDAK RSKLSVLLID NLERLIDYHP VGPRYSNLVI
710 720 730 740 750
QALLVLLNAP PPAGHRLFVI ATSSDRMFLR DMGLMDVFGD VIDIPKLTTA
760 770 780 790 800
GQMMNVIQES NIYSDDQLPM IEQKLASICR GEGFHGVGIK HLLELIESAR
810 820
QCEADYRVPT LLNMMEGLAL NLYR
Length:824
Mass (Da):91,335
Last modified:May 30, 2000 - v2
Checksum:i67232C5115B0B03A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z96100, Z79698 Genomic DNA. Translation: CAB09531.1.
PIRiT23096.
RefSeqiNP_001076603.1. NM_001083134.3.
UniGeneiCel.17352.

Genome annotation databases

EnsemblMetazoaiH15N14.2a; H15N14.2a; WBGene00003818.
GeneIDi266842.
KEGGicel:CELE_H15N14.2.
UCSCiH15N14.2a. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z96100, Z79698 Genomic DNA. Translation: CAB09531.1.
PIRiT23096.
RefSeqiNP_001076603.1. NM_001083134.3.
UniGeneiCel.17352.

3D structure databases

ProteinModelPortaliQ94392.
SMRiQ94392. Positions 66-815.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi57330. 5 interactions.
DIPiDIP-24326N.
IntActiQ94392. 4 interactions.
MINTiMINT-212260.

Proteomic databases

PaxDbiQ94392.
PRIDEiQ94392.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiH15N14.2a; H15N14.2a; WBGene00003818.
GeneIDi266842.
KEGGicel:CELE_H15N14.2.
UCSCiH15N14.2a. c. elegans.

Organism-specific databases

CTDi266842.
WormBaseiH15N14.2a; CE19925; WBGene00003818; nsf-1.

Phylogenomic databases

eggNOGiCOG0464.
GeneTreeiENSGT00530000064085.
HOGENOMiHOG000198544.
InParanoidiQ94392.
KOiK06027.
OMAiHDEDPAN.
PhylomeDBiQ94392.

Miscellaneous databases

NextBioi953236.
PROiQ94392.

Gene expression databases

ExpressionAtlasiQ94392. baseline.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiNSF_CAEEL
AccessioniPrimary (citable) accession number: Q94392
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 30, 2000
Last modified: March 4, 2015
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.