Q94361 (GEI17_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E3 SUMO-protein ligase gei-17
EC=6.3.2.-

Alternative name(s):
Gex-3-interacting protein 17

Gene names

Name:	gei-17
ORF Names:	W10D5.3

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	793 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as an E3-type smo-1 ligase. In the early embryo, specifically suppresses checkpoint activation in response to DNA damage, maybe by promoting mus-101 sumoylation. Ref.3 Ref.4 Ref.5
Pathway	Protein modification; protein sumoylation.
Subunit structure	May interact with gex-3.
Sequence similarities	Belongs to the PIAS family. Contains 1 PINIT domain. Contains 1 SP-RING-type zinc finger.

Ontologies

Keywords
Biological process	DNA damage DNA repair Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW body morphogenesis Inferred from mutant phenotype. Source: WormBase embryo development ending in birth or egg hatching Inferred from mutant phenotype Ref.2. Source: WormBase hermaphrodite genitalia development Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase positive regulation of multicellular organism growth Inferred from mutant phenotype. Source: WormBase protein sumoylation Inferred from direct assay Ref.3. Source: WormBase
Molecular function	RNA polymerase II transcription factor binding Inferred from physical interaction Ref.4. Source: WormBase SUMO ligase activity Inferred from direct assay Ref.3. Source: WormBase zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q94361-1)

Also known as: c;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: Q94361-2)

Also known as: f;

The sequence of this isoform differs from the canonical sequence as follows:
     165-219: Missing.
     776-793: GAFAYYPPQYPQQQYRQN → DTNNIQPPRILSMAEIQANASRLLVNGHLMIDHSGVTMRNPRGSRN

Note: No experimental confirmation available.

Isoform 3 (identifier: Q94361-3)

Also known as: g;

The sequence of this isoform differs from the canonical sequence as follows:
     98-219: Missing.
     674-674: Missing.
     776-793: GAFAYYPPQYPQQQYRQN → DTNNIQPPRILSMAEIQANASRLLVNGHLMIDHSGVTMRNPRGSRN

Note: No experimental confirmation available.

Isoform 4 (identifier: Q94361-4)

Also known as: e;

The sequence of this isoform differs from the canonical sequence as follows:
     165-219: Missing.
     572-587: Missing.
     674-719: Missing.

Note: No experimental confirmation available.

Isoform 5 (identifier: Q94361-5)

Also known as: a;

The sequence of this isoform differs from the canonical sequence as follows:
     165-219: Missing.
     674-719: Missing.

Note: No experimental confirmation available.

Isoform 6 (identifier: Q94361-6)

Also known as: d;

The sequence of this isoform differs from the canonical sequence as follows:
     98-99: Missing.
     165-219: Missing.
     674-719: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 793	793	E3 SUMO-protein ligase gei-17		PRO_0000270191
Regions
	Domain	216 – 380	165	PINIT
	Zinc finger	413 – 490	78	SP-RING-type
	Compositional bias	131 – 159	29	Gln-rich
	Compositional bias	185 – 191	7	Poly-Ala
	Compositional bias	519 – 524	6	Poly-Asp
	Compositional bias	563 – 566	4	Poly-Asp
	Compositional bias	724 – 792	69	Gln-rich
Natural variations
	Alternative sequence	98 – 219	122	Missing in isoform 3.		VSP_022185
	Alternative sequence	98 – 99	2	Missing in isoform 6.		VSP_022186
	Alternative sequence	165 – 219	55	Missing in isoform 2, isoform 4, isoform 5 and isoform 6.		VSP_022187
	Alternative sequence	572 – 587	16	Missing in isoform 4.		VSP_022188
	Alternative sequence	674 – 719	46	Missing in isoform 4, isoform 5 and isoform 6.		VSP_022189
	Alternative sequence	674	1	Missing in isoform 3.		VSP_022190
	Alternative sequence	776 – 793	18	GAFAY…QYRQN → DTNNIQPPRILSMAEIQANA SRLLVNGHLMIDHSGVTMRN PRGSRN in isoform 2 and isoform 3.		VSP_022191

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (c) [UniParc]. Last modified December 16, 2008. Version 3. Checksum: 1E4AAC70354B4DD8	FASTA	793	89,575
	10 20 30 40 50 60 MLPNNQWQIP INNGLTHQEN MAAHAAVMKL RVHDLQSIIS QLSLRKPRPQ KSEHQKVVVE 70 80 90 100 110 120 SLRDPHHARQ IYQMVICFSP LIFSSLQASN FPNGNYEMQK RPATTSQVRS HPYVLPSRSG 130 140 150 160 170 180 ASNHLVNHHY QQQQQQQPQP HNLLHQQMMA SHHSHLQQQH HPSTVRWLTP ELLEEQLRGS 190 200 210 220 230 240 MRYGAPAAAA ATNAPLHSSF PNHGRSSQQS LQKSEKSNRP KKMYADNFEP LPLPFYDVIS 250 260 270 280 290 300 VLLKPVELHS SDSPTLKQTK QLQFPFLLTA EHISKISYRA DVTPLPRYEL QLRFFNLTEP 310 320 330 340 350 360 VQGPQKDDFP LNCYARVDDS VVQLPNVIPT NKTNAEPKRP SRPVNITSNM NRYKKEHTVA 370 380 390 400 410 420 VEWLADKRVW AAGVYFVHRV NSDILFKRLN QNVSRHRSLE VTKQEVIKKL SGGEDDIAMD 430 440 450 460 470 480 RLNISLLDPL CKTRMTTPSR CQDCTHLQCF DLLSYLMMNE KKPTWQCPVC SSNCPYDRLI 490 500 510 520 530 540 VDDYFLDMLA KVDKNTTEVE LKEDGSYDVI KEEAFCISDD DDDDVVPATV NGTASCSSTN 550 560 570 580 590 600 GNGLANEAAK KKPADDDIIT LSDDDDEELN RGIMNSLNDS FSPGRHTASA ELAAQKTPPQ 610 620 630 640 650 660 QKKKTKDDDI EIITLDDTPP RPVAASANLP MRQMSQQNQM PVGSSPSGMA STQMGMNEGA 670 680 690 700 710 720 SKTIRDALNK IGEQSANSST QSSPLVQLHH TTHPLNFAQS SYMNPSSGSQ TPTSQYGYSP 730 740 750 760 770 780 MINQAPHFQM QNGLIGRNNQ MVHMQQHHLQ QQQQQQQSPQ IMSPSFYAQQ QMSNGGAFAY 790 YPPQYPQQQY RQN
	Isoform 2 (f) [UniParc]. Checksum: 7089047E168EE5B8	FASTA	766	86,407
	10 20 30 40 50 60 MLPNNQWQIP INNGLTHQEN MAAHAAVMKL RVHDLQSIIS QLSLRKPRPQ KSEHQKVVVE 70 80 90 100 110 120 SLRDPHHARQ IYQMVICFSP LIFSSLQASN FPNGNYEMQK RPATTSQVRS HPYVLPSRSG 130 140 150 160 170 180 ASNHLVNHHY QQQQQQQPQP HNLLHQQMMA SHHSHLQQQH HPSTPKKMYA DNFEPLPLPF 190 200 210 220 230 240 YDVISVLLKP VELHSSDSPT LKQTKQLQFP FLLTAEHISK ISYRADVTPL PRYELQLRFF 250 260 270 280 290 300 NLTEPVQGPQ KDDFPLNCYA RVDDSVVQLP NVIPTNKTNA EPKRPSRPVN ITSNMNRYKK 310 320 330 340 350 360 EHTVAVEWLA DKRVWAAGVY FVHRVNSDIL FKRLNQNVSR HRSLEVTKQE VIKKLSGGED 370 380 390 400 410 420 DIAMDRLNIS LLDPLCKTRM TTPSRCQDCT HLQCFDLLSY LMMNEKKPTW QCPVCSSNCP 430 440 450 460 470 480 YDRLIVDDYF LDMLAKVDKN TTEVELKEDG SYDVIKEEAF CISDDDDDDV VPATVNGTAS 490 500 510 520 530 540 CSSTNGNGLA NEAAKKKPAD DDIITLSDDD DEELNRGIMN SLNDSFSPGR HTASAELAAQ 550 560 570 580 590 600 KTPPQQKKKT KDDDIEIITL DDTPPRPVAA SANLPMRQMS QQNQMPVGSS PSGMASTQMG 610 620 630 640 650 660 MNEGASKTIR DALNKIGEQS ANSSTQSSPL VQLHHTTHPL NFAQSSYMNP SSGSQTPTSQ 670 680 690 700 710 720 YGYSPMINQA PHFQMQNGLI GRNNQMVHMQ QHHLQQQQQQ QQSPQIMSPS FYAQQQMSNG 730 740 750 760 DTNNIQPPRI LSMAEIQANA SRLLVNGHLM IDHSGVTMRN PRGSRN
	Isoform 3 (g) [UniParc]. Checksum: 0F6E6D8516ACD1BE	FASTA	698	78,474
	10 20 30 40 50 60 MLPNNQWQIP INNGLTHQEN MAAHAAVMKL RVHDLQSIIS QLSLRKPRPQ KSEHQKVVVE 70 80 90 100 110 120 SLRDPHHARQ IYQMVICFSP LIFSSLQASN FPNGNYEPKK MYADNFEPLP LPFYDVISVL 130 140 150 160 170 180 LKPVELHSSD SPTLKQTKQL QFPFLLTAEH ISKISYRADV TPLPRYELQL RFFNLTEPVQ 190 200 210 220 230 240 GPQKDDFPLN CYARVDDSVV QLPNVIPTNK TNAEPKRPSR PVNITSNMNR YKKEHTVAVE 250 260 270 280 290 300 WLADKRVWAA GVYFVHRVNS DILFKRLNQN VSRHRSLEVT KQEVIKKLSG GEDDIAMDRL 310 320 330 340 350 360 NISLLDPLCK TRMTTPSRCQ DCTHLQCFDL LSYLMMNEKK PTWQCPVCSS NCPYDRLIVD 370 380 390 400 410 420 DYFLDMLAKV DKNTTEVELK EDGSYDVIKE EAFCISDDDD DDVVPATVNG TASCSSTNGN 430 440 450 460 470 480 GLANEAAKKK PADDDIITLS DDDDEELNRG IMNSLNDSFS PGRHTASAEL AAQKTPPQQK 490 500 510 520 530 540 KKTKDDDIEI ITLDDTPPRP VAASANLPMR QMSQQNQMPV GSSPSGMAST QMGMNEGASK 550 560 570 580 590 600 TIRDALNKIG ESANSSTQSS PLVQLHHTTH PLNFAQSSYM NPSSGSQTPT SQYGYSPMIN 610 620 630 640 650 660 QAPHFQMQNG LIGRNNQMVH MQQHHLQQQQ QQQQSPQIMS PSFYAQQQMS NGDTNNIQPP 670 680 690 RILSMAEIQA NASRLLVNGH LMIDHSGVTM RNPRGSRN
	Isoform 4 (e) [UniParc]. Checksum: 6DA74CC55A10A004	FASTA	676	76,891
	10 20 30 40 50 60 MLPNNQWQIP INNGLTHQEN MAAHAAVMKL RVHDLQSIIS QLSLRKPRPQ KSEHQKVVVE 70 80 90 100 110 120 SLRDPHHARQ IYQMVICFSP LIFSSLQASN FPNGNYEMQK RPATTSQVRS HPYVLPSRSG 130 140 150 160 170 180 ASNHLVNHHY QQQQQQQPQP HNLLHQQMMA SHHSHLQQQH HPSTPKKMYA DNFEPLPLPF 190 200 210 220 230 240 YDVISVLLKP VELHSSDSPT LKQTKQLQFP FLLTAEHISK ISYRADVTPL PRYELQLRFF 250 260 270 280 290 300 NLTEPVQGPQ KDDFPLNCYA RVDDSVVQLP NVIPTNKTNA EPKRPSRPVN ITSNMNRYKK 310 320 330 340 350 360 EHTVAVEWLA DKRVWAAGVY FVHRVNSDIL FKRLNQNVSR HRSLEVTKQE VIKKLSGGED 370 380 390 400 410 420 DIAMDRLNIS LLDPLCKTRM TTPSRCQDCT HLQCFDLLSY LMMNEKKPTW QCPVCSSNCP 430 440 450 460 470 480 YDRLIVDDYF LDMLAKVDKN TTEVELKEDG SYDVIKEEAF CISDDDDDDV VPATVNGTAS 490 500 510 520 530 540 CSSTNGNGLA NEAAKKKPAD DDIITLSDDD DEELNRASAE LAAQKTPPQQ KKKTKDDDIE 550 560 570 580 590 600 IITLDDTPPR PVAASANLPM RQMSQQNQMP VGSSPSGMAS TQMGMNEGAS KTIRDALNKI 610 620 630 640 650 660 GEPMINQAPH FQMQNGLIGR NNQMVHMQQH HLQQQQQQQQ SPQIMSPSFY AQQQMSNGGA 670 FAYYPPQYPQ QQYRQN
	Isoform 5 (a) [UniParc]. Checksum: 8D68645C934B89CC	FASTA	692	78,606
	10 20 30 40 50 60 MLPNNQWQIP INNGLTHQEN MAAHAAVMKL RVHDLQSIIS QLSLRKPRPQ KSEHQKVVVE 70 80 90 100 110 120 SLRDPHHARQ IYQMVICFSP LIFSSLQASN FPNGNYEMQK RPATTSQVRS HPYVLPSRSG 130 140 150 160 170 180 ASNHLVNHHY QQQQQQQPQP HNLLHQQMMA SHHSHLQQQH HPSTPKKMYA DNFEPLPLPF 190 200 210 220 230 240 YDVISVLLKP VELHSSDSPT LKQTKQLQFP FLLTAEHISK ISYRADVTPL PRYELQLRFF 250 260 270 280 290 300 NLTEPVQGPQ KDDFPLNCYA RVDDSVVQLP NVIPTNKTNA EPKRPSRPVN ITSNMNRYKK 310 320 330 340 350 360 EHTVAVEWLA DKRVWAAGVY FVHRVNSDIL FKRLNQNVSR HRSLEVTKQE VIKKLSGGED 370 380 390 400 410 420 DIAMDRLNIS LLDPLCKTRM TTPSRCQDCT HLQCFDLLSY LMMNEKKPTW QCPVCSSNCP 430 440 450 460 470 480 YDRLIVDDYF LDMLAKVDKN TTEVELKEDG SYDVIKEEAF CISDDDDDDV VPATVNGTAS 490 500 510 520 530 540 CSSTNGNGLA NEAAKKKPAD DDIITLSDDD DEELNRGIMN SLNDSFSPGR HTASAELAAQ 550 560 570 580 590 600 KTPPQQKKKT KDDDIEIITL DDTPPRPVAA SANLPMRQMS QQNQMPVGSS PSGMASTQMG 610 620 630 640 650 660 MNEGASKTIR DALNKIGEPM INQAPHFQMQ NGLIGRNNQM VHMQQHHLQQ QQQQQQSPQI 670 680 690 MSPSFYAQQQ MSNGGAFAYY PPQYPQQQYR QN
	Isoform 6 (d) [UniParc]. Checksum: 22D4B711EEDA3121	FASTA	690	78,346
	10 20 30 40 50 60 MLPNNQWQIP INNGLTHQEN MAAHAAVMKL RVHDLQSIIS QLSLRKPRPQ KSEHQKVVVE 70 80 90 100 110 120 SLRDPHHARQ IYQMVICFSP LIFSSLQASN FPNGNYEKRP ATTSQVRSHP YVLPSRSGAS 130 140 150 160 170 180 NHLVNHHYQQ QQQQQPQPHN LLHQQMMASH HSHLQQQHHP STPKKMYADN FEPLPLPFYD 190 200 210 220 230 240 VISVLLKPVE LHSSDSPTLK QTKQLQFPFL LTAEHISKIS YRADVTPLPR YELQLRFFNL 250 260 270 280 290 300 TEPVQGPQKD DFPLNCYARV DDSVVQLPNV IPTNKTNAEP KRPSRPVNIT SNMNRYKKEH 310 320 330 340 350 360 TVAVEWLADK RVWAAGVYFV HRVNSDILFK RLNQNVSRHR SLEVTKQEVI KKLSGGEDDI 370 380 390 400 410 420 AMDRLNISLL DPLCKTRMTT PSRCQDCTHL QCFDLLSYLM MNEKKPTWQC PVCSSNCPYD 430 440 450 460 470 480 RLIVDDYFLD MLAKVDKNTT EVELKEDGSY DVIKEEAFCI SDDDDDDVVP ATVNGTASCS 490 500 510 520 530 540 STNGNGLANE AAKKKPADDD IITLSDDDDE ELNRGIMNSL NDSFSPGRHT ASAELAAQKT 550 560 570 580 590 600 PPQQKKKTKD DDIEIITLDD TPPRPVAASA NLPMRQMSQQ NQMPVGSSPS GMASTQMGMN 610 620 630 640 650 660 EGASKTIRDA LNKIGEPMIN QAPHFQMQNG LIGRNNQMVH MQQHHLQQQQ QQQQSPQIMS 670 680 690 PSFYAQQQMS NGGAFAYYPP QYPQQQYRQN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[2]	"Isolation of the interacting molecules with GEX-3 by a novel functional screening." Tsuboi D., Qadota H., Kasuya K., Amano M., Kaibuchi K. Biochem. Biophys. Res. Commun. 292:697-701(2002) [PubMed: 11922622] [Abstract] Cited for: POSSIBLE INTERACTION WITH GEX-3.
[3]	"Systematic, RNA-interference-mediated identification of mus-101 modifier genes in Caenorhabditis elegans." Holway A.H., Hung C., Michael W.M. Genetics 169:1451-1460(2005) [PubMed: 15654100] [Abstract] Cited for: FUNCTION.
[4]	"The T-box factor TBX-2 and the SUMO conjugating enzyme UBC-9 are required for ABa-derived pharyngeal muscle in C. elegans." Roy Chowdhuri S., Crum T., Woollard A., Aslam S., Okkema P.G. Dev. Biol. 295:664-677(2006) [PubMed: 16701625] [Abstract] Cited for: FUNCTION.
[5]	"Checkpoint silencing during the DNA damage response in Caenorhabditis elegans embryos." Holway A.H., Kim S.-H., La Volpe A., Michael W.M. J. Cell Biol. 172:999-1008(2006) [PubMed: 16549501] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z79758 Genomic DNA. Translation: CAB02133.3. Z79758 Genomic DNA. Translation: CAB02134.2. Z79758 Genomic DNA. Translation: CAB54321.2. Z79758 Genomic DNA. Translation: CAD98729.2. Z79758 Genomic DNA. Translation: CAD98730.2. Z79758 Genomic DNA. Translation: CAI79179.2.
PIR	T26330. T26331. T26332.
RefSeq	NP_001021677.2. NM_001026506.2. NP_001021678.2. NM_001026507.2. NP_001021679.2. NM_001026508.2. NP_492442.2. NM_060041.2. NP_492443.2. NM_060042.2. NP_492444.3. NM_060043.3.
UniGene	Cel.5457.
3D structure databases
ProteinModelPortal	Q94361.
SMR	Q94361. Positions 228-510.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-24916N.
IntAct	Q94361. 7 interactions.
MINT	MINT-1066216.
STRING	Q94361.
Proteomic databases
PRIDE	Q94361.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	W10D5.3c; W10D5.3c; W10D5.3.
GeneID	172733.
KEGG	cel:W10D5.3.
NMPDR	fig|6239.3.peg.2408.
UCSC	W10D5.3f. c. elegans.
Organism-specific databases
CTD	172733.
WormBase	W10D5.3a; CE42759; WBGene00001574; gei-17. W10D5.3c; CE42756; WBGene00001574; gei-17. W10D5.3d; CE42726; WBGene00001574; gei-17. W10D5.3e; CE42813; WBGene00001574; gei-17. W10D5.3f; CE42789; WBGene00001574; gei-17. W10D5.3g; CE42772; WBGene00001574; gei-17.
Phylogenomic databases
eggNOG	meNOG06967.
GeneTree	EMGT00050000001426.
HOGENOM	HBG377796.
InParanoid	Q94361.
PhylomeDB	Q94361.
Gene expression databases
ArrayExpress	Q94361.
Family and domain databases
InterPro	IPR023321. PINIT. IPR004181. Znf_MIZ. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Gene3D	G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 1 hit.
KO	K04706.
Pfam	PF02891. zf-MIZ. 1 hit. [Graphical view]
PROSITE	PS51466. PINIT. 1 hit. PS51044. ZF_SP_RING. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	876777.

Entry information

Entry name

GEI17_CAEEL

Accession

Primary (citable) accession number: Q94361
Secondary accession number(s): Q564X2 , Q7Z0X3, Q7Z0X4, Q94363, Q9U326

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	December 16, 2008
Last modified:	January 25, 2012
This is version 79 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project