Reviewed, UniProtKB/Swiss-Prot Q94360 (NDUS7_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
    EC=1.6.5.3
    EC=1.6.99.3
Alternative name(s):
    NADH-ubiquinone oxidoreductase 20 kDa subunit
    Complex I-20kD
      Short name=CI-20kD

Gene names

ORF Names:

W10D5.2

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	199 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol. NADH + acceptor = NAD+ + reduced acceptor.
Cofactor	Binds 1 4Fe-4S cluster Potential.
Subunit structure	Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the complex I 20 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	body morphogenesis Inferred from mutant phenotype. Source: WormBase electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase hermaphrodite genitalia development Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase positive regulation of multicellular organism growth Inferred from mutant phenotype. Source: WormBase transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 199		Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial		PRO_0000020030
Sites
	Metal binding	74	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	75	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	139	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	169	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q94360-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: B40B33385A7DB667	FASTA	199	21,912
	10 20 30 40 50 60 MLSALRTAGA VGTRRLASTQ AIASNSEAPK GIATTGTPFL NPSSKAEYAL ARLDDVLNLA 70 80 90 100 110 120 QRGSIWPLTF GLACCAVEMM HFAAPRYDMD RYGVVFRASP RQADLIFVAG TVTNKMAPAL 130 140 150 160 170 180 RRIYDQMPEA KWVISMGSCA NGGGYYHYAY SVLRGCDRVI PVDIYVPGCP PTAEALLYGV 190 LQLQKKIKRK REAQLWYRR

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z79758 Genomic DNA. Translation: CAB02132.1.
PIR	T26329.
RefSeq	NP_492445.1.
UniGene	Cel.18304
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	W10D5.2. Caenorhabditis elegans. [Contig view]
GeneID	172734.
KEGG	cel:W10D5.2.
Organism-specific databases
WormBase	WBGene00012376. W10D5.2.
WormPep	W10D5.2. CE14780. [WorfDB]
Phylogenomic databases
OMA	Q94360. AIASNSE.
Enzyme and pathway databases
BRENDA	1.6.5.3. 672. 1.6.99.3. 672.
Gene expression databases
ArrayExpress	Q94360.
Family and domain databases
InterPro	IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR006138. NADH_UbQ_OxRdtase_su-20kDa. IPR014406. NiFe_hyd_3_ssu/Q_oxred_NuoB. [Graphical view]
PANTHER	PTHR11995:SF2. NADH_DH_20kDa. 1 hit. PTHR11995. NiFe_hyd_3_ssu/Q_oxred_NuoB. 1 hit.
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
TIGRFAMs	TIGR01957. nuoB_fam. 1 hit.
PROSITE	PS01150. COMPLEX1_20K. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	876791.

Entry information

Entry name

NDUS7_CAEEL

Accession

Primary (citable) accession number: Q94360

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project