ID   P2C01_ORYSJ             Reviewed;         331 AA.
AC   Q942P9; A0A0P0UYM8;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   27-MAR-2024, entry version 127.
DE   RecName: Full=Probable protein phosphatase 2C 1;
DE            Short=OsPP2C01;
DE            EC=3.1.3.16;
GN   OrderedLocusNames=Os01g0164600, LOC_Os01g07090;
GN   ORFNames=B1189A09.48, OsJ_000491, P0701D05.3;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447438; DOI=10.1038/nature01184;
RA   Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA   Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA   Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA   Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA   Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA   Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA   Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA   Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA   Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA   Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA   Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA   Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA   Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT   "The genome sequence and structure of rice chromosome 1.";
RL   Nature 420:312-316(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [7]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=19021904; DOI=10.1186/1471-2164-9-550;
RA   Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.;
RT   "Genome-wide and expression analysis of protein phosphatase 2C in rice and
RT   Arabidopsis.";
RL   BMC Genomics 9:550-550(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O-phospho-L-seryl-[protein] = L-seryl-[protein] +
CC         phosphate; Xref=Rhea:RHEA:20629, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC         COMP:11604, ChEBI:CHEBI:15377, ChEBI:CHEBI:29999, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:83421; EC=3.1.3.16;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] +
CC         phosphate; Xref=Rhea:RHEA:47004, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC         COMP:11605, ChEBI:CHEBI:15377, ChEBI:CHEBI:30013, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:61977; EC=3.1.3.16;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Note=Binds 2 magnesium or manganese ions per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the PP2C family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AK099645; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AP003209; BAC00581.1; -; Genomic_DNA.
DR   EMBL; AP003301; BAB64790.1; -; Genomic_DNA.
DR   EMBL; AP008207; BAF04017.1; -; Genomic_DNA.
DR   EMBL; AP014957; BAS70557.1; -; Genomic_DNA.
DR   EMBL; CM000138; EAZ10666.1; -; Genomic_DNA.
DR   EMBL; AK099645; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; XP_015626680.1; XM_015771194.1.
DR   PDB; 6AE9; X-ray; 1.47 A; A/B=44-294.
DR   PDBsum; 6AE9; -.
DR   AlphaFoldDB; Q942P9; -.
DR   SMR; Q942P9; -.
DR   STRING; 39947.Q942P9; -.
DR   PaxDb; 39947-Q942P9; -.
DR   EnsemblPlants; Os01t0164600-01; Os01t0164600-01; Os01g0164600.
DR   GeneID; 4324414; -.
DR   Gramene; Os01t0164600-01; Os01t0164600-01; Os01g0164600.
DR   KEGG; osa:4324414; -.
DR   eggNOG; KOG1379; Eukaryota.
DR   HOGENOM; CLU_029404_3_3_1; -.
DR   InParanoid; Q942P9; -.
DR   OMA; CGFPKDF; -.
DR   OrthoDB; 240602at2759; -.
DR   Proteomes; UP000000763; Chromosome 1.
DR   Proteomes; UP000007752; Chromosome 1.
DR   Proteomes; UP000059680; Chromosome 1.
DR   GO; GO:0009507; C:chloroplast; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0017018; F:myosin phosphatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004722; F:protein serine/threonine phosphatase activity; IBA:GO_Central.
DR   Gene3D; 3.60.40.10; PPM-type phosphatase domain; 1.
DR   InterPro; IPR036457; PPM-type-like_dom_sf.
DR   InterPro; IPR001932; PPM-type_phosphatase-like_dom.
DR   InterPro; IPR039123; PPTC7.
DR   PANTHER; PTHR12320; PROTEIN PHOSPHATASE 2C; 1.
DR   PANTHER; PTHR12320:SF60; PROTEIN PHOSPHATASE 2C 26-RELATED; 1.
DR   SMART; SM00331; PP2C_SIG; 1.
DR   SMART; SM00332; PP2Cc; 1.
DR   SUPFAM; SSF81606; PP2C-like; 1.
DR   PROSITE; PS51746; PPM_2; 1.
DR   Genevisible; Q942P9; OS.
PE   1: Evidence at protein level;
KW   3D-structure; Hydrolase; Magnesium; Manganese; Metal-binding;
KW   Protein phosphatase; Reference proteome.
FT   CHAIN           1..331
FT                   /note="Probable protein phosphatase 2C 1"
FT                   /id="PRO_0000363247"
FT   DOMAIN          48..292
FT                   /note="PPM-type phosphatase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01082"
FT   REGION          1..29
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          300..331
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..15
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        315..331
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         79
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         79
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         80
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         210
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         283
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   STRAND          44..51
FT                   /evidence="ECO:0007829|PDB:6AE9"
FT   HELIX           54..59
FT                   /evidence="ECO:0007829|PDB:6AE9"
FT   STRAND          62..68
FT                   /evidence="ECO:0007829|PDB:6AE9"
FT   STRAND          70..72
FT                   /evidence="ECO:0007829|PDB:6AE9"
FT   STRAND          74..80
FT                   /evidence="ECO:0007829|PDB:6AE9"
FT   HELIX           84..87
FT                   /evidence="ECO:0007829|PDB:6AE9"
FT   HELIX           93..105
FT                   /evidence="ECO:0007829|PDB:6AE9"
FT   TURN            109..113
FT                   /evidence="ECO:0007829|PDB:6AE9"
FT   HELIX           115..125
FT                   /evidence="ECO:0007829|PDB:6AE9"
FT   STRAND          131..139
FT                   /evidence="ECO:0007829|PDB:6AE9"
FT   STRAND          143..152
FT                   /evidence="ECO:0007829|PDB:6AE9"
FT   STRAND          154..159
FT                   /evidence="ECO:0007829|PDB:6AE9"
FT   STRAND          162..166
FT                   /evidence="ECO:0007829|PDB:6AE9"
FT   STRAND          171..173
FT                   /evidence="ECO:0007829|PDB:6AE9"
FT   STRAND          180..183
FT                   /evidence="ECO:0007829|PDB:6AE9"
FT   HELIX           189..191
FT                   /evidence="ECO:0007829|PDB:6AE9"
FT   STRAND          193..198
FT                   /evidence="ECO:0007829|PDB:6AE9"
FT   STRAND          204..208
FT                   /evidence="ECO:0007829|PDB:6AE9"
FT   HELIX           210..213
FT                   /evidence="ECO:0007829|PDB:6AE9"
FT   HELIX           218..226
FT                   /evidence="ECO:0007829|PDB:6AE9"
FT   HELIX           231..246
FT                   /evidence="ECO:0007829|PDB:6AE9"
FT   HELIX           254..261
FT                   /evidence="ECO:0007829|PDB:6AE9"
FT   TURN            262..265
FT                   /evidence="ECO:0007829|PDB:6AE9"
FT   HELIX           267..273
FT                   /evidence="ECO:0007829|PDB:6AE9"
FT   STRAND          285..292
FT                   /evidence="ECO:0007829|PDB:6AE9"
SQ   SEQUENCE   331 AA;  35426 MW;  BB7431DA8BEA6432 CRC64;
     MAASSTATRL SPPRLHAPTT PSPHLPLRRS RFSPLRAAKL EAVLTIGTHL IPHPRKAETG
     GEDAFFVNGD DGGVFAVADG VSGWAEKDVN PALFSRELMA HTSTFLKDEE VNHDPQLLLM
     KAHAATTSVG SATVIIAMLE KTGILKIASV GDCGLKVIRK GQVMFSTCPQ EHYFDCPYQL
     SSEAIGQTYL DALVCTVNLM EGDMIVSGSD GFFDNIFDQE IVSVISESPG VDEAAKALAE
     LARKHSVDVT FDSPYSMEAR SRGFDVPSWK KFIGGKLIGG KMDDITVIVA QVKAVMIPDD
     EGVDEEKGQG DEQGSAVAVA SSEQKEDSIT T
//