Reviewed, UniProtKB/Swiss-Prot Q941X2 (LAC3_ORYSJ)

Names and origin

Protein names

Recommended name:
    Laccase-3
    EC=1.10.3.2
Alternative name(s):
    Benzenediol:oxygen oxidoreductase 3
    Urishiol oxidase 3
    Diphenol oxidase 3

Gene names

Name:	LAC3
Ordered Locus Names:	Os01g0827300, LOC_Os01g61160
ORF Names:	B1088C09.5

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	567 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Lignin degradation and detoxification of lignin-derived products By similarity.
Catalytic activity	4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.
Cofactor	Binds 4 copper ions per monomer By similarity.
Subcellular location	Secreted › extracellular space › apoplast Potential.
Sequence similarities	Belongs to the multicopper oxidase family. Contains 3 plastocyanin-like domains.

Ontologies

Keywords
Biological process	Lignin degradation
Cellular component	Apoplast Secreted
Domain	Repeat Signal
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW laccase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 567	543	Laccase-3		PRO_0000291888
Regions
	Domain	32 – 148	117	Plastocyanin-like 1
	Domain	158 – 310	153	Plastocyanin-like 2
	Domain	415 – 551	137	Plastocyanin-like 3
Sites
	Metal binding	82	1	Copper 1 By similarity
	Metal binding	84	1	Copper 2 By similarity
	Metal binding	127	1	Copper 2 By similarity
	Metal binding	129	1	Copper 3 By similarity
	Metal binding	468	1	Copper 4 By similarity
	Metal binding	471	1	Copper 1 By similarity
	Metal binding	473	1	Copper 3 By similarity
	Metal binding	530	1	Copper 3 By similarity
	Metal binding	531	1	Copper 4 By similarity
	Metal binding	532	1	Copper 2 By similarity
	Metal binding	536	1	Copper 4 By similarity
Amino acid modifications
	Glycosylation	78	1	N-linked (GlcNAc...) Potential
	Glycosylation	148	1	N-linked (GlcNAc...) Potential
	Glycosylation	187	1	N-linked (GlcNAc...) Potential
	Glycosylation	203	1	N-linked (GlcNAc...) Potential
	Glycosylation	298	1	N-linked (GlcNAc...) Potential
	Glycosylation	330	1	N-linked (GlcNAc...) Potential
	Glycosylation	379	1	N-linked (GlcNAc...) Potential
	Glycosylation	389	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q941X2-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: FA8603416C584361	FASTA	567	62,646
	10 20 30 40 50 60 MASSSSSRLL FLLSCSVLAL LAGAEVHHHE FIVQETPVKR LCKTHNVITV NGQLPGPTLE 70 80 90 100 110 120 VREGDTVVIN VVNHAQYNVT IHWHGIRQFR TGWADGPEFV TQCPIKPGGS YKYRFTIEGQ 130 140 150 160 170 180 EGTLWWHAHS SWLRATVYGA LIIRPRENKT YPFEKPAREV PLILGEWWDA DPIQVIREAQ 190 200 210 220 230 240 RTGAAPNISD AYTINGQPGD LYNCSKEETT AVPVKPGETA LLRFINAALN QELFVSIAQH 250 260 270 280 290 300 KMTVVGVDAS YTKPFTTSVL MIAPGQTTDV LVTMDQAPTR YYLAARAYDS AQGVAFDNTT 310 320 330 340 350 360 TTAVIEYDCG CATDFGPSIP PAFPVLPAFN DTNTATAFAA GIRSPHEVKI PGPVDENLFF 370 380 390 400 410 420 TVGVGLFNCE PGQQCGGPNN TRFTASMNNI SFVFPQTTSL LHAHYYGIPG VFTTDFPAYP 430 440 450 460 470 480 PVQFDYTAQN VPRYLWQPVP ATKLYKLKFG SVVQIVLQDT SIVSPENHPI HIHGYDFYIL 490 500 510 520 530 540 AEGFGNFDPK KDAKKFNYVD PPQRNTVAVP TNGWAVIRFV ADNPGVWLMH CHLDVHITWG 550 560 LAMAFLVEDG YGKLETLEAP PVDLPMC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP003734 Genomic DNA. Translation: BAB68098.1. AP008207 Genomic DNA. Translation: BAF06593.1. AK071929 mRNA. No translation available.
RefSeq	NP_001044679.1.
UniGene	Os.34310
3D structure databases
HSSP	HSSP built from PDB template 1KV7 based on UniProtKB P36649.
ModBase	Search...
Genome annotation databases
GeneID	4327522.
KEGG	osa:4327522.
NMPDR	fig|39947.1.peg.7371.
Organism-specific databases
Gramene	Q941X2.
Family and domain databases
InterPro	IPR001117. Cu-oxidase. IPR011706. Cu-oxidase_2. IPR011707. Cu-oxidase_3. IPR002355. Cu_oxidase_Cu_BS. IPR008972. Cupredoxin. IPR017761. Laccase. [Graphical view]
Gene3D	G3DSA:2.60.40.420. Cupredoxin. 3 hits.
Pfam	PF00394. Cu-oxidase. 1 hit. PF07731. Cu-oxidase_2. 1 hit. PF07732. Cu-oxidase_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR03389. laccase. 1 hit.
PROSITE	PS00079. MULTICOPPER_OXIDASE1. 1 hit. PS00080. MULTICOPPER_OXIDASE2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAC3_ORYSJ

Accession

Primary (citable) accession number: Q941X2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	December 1, 2001
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)