Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphatidylinositol 3-kinase age-1

Gene

age-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphatidylinositol 3-kinase homolog that regulates longevity and diapause. Could function in the development or neuroendocrine signaling of the dauer pathway (PubMed:8700226). Mediates susceptibility to enteropathogenic E.coli infection (PubMed:16091039).2 Publications

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.

GO - Molecular functioni

  • 1-phosphatidylinositol-3-kinase activity Source: UniProtKB
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

  • aging Source: UniProtKB
  • dauer entry Source: WormBase
  • dauer larval development Source: UniProtKB
  • determination of adult lifespan Source: WormBase
  • embryo development ending in birth or egg hatching Source: WormBase
  • insulin receptor signaling pathway involved in determination of adult lifespan Source: WormBase
  • learning or memory Source: WormBase
  • phosphatidylinositol-3-phosphate biosynthetic process Source: GOC
  • phosphatidylinositol-mediated signaling Source: InterPro
  • regulation of chemotaxis Source: WormBase
  • reproduction Source: WormBase
  • response to cadmium ion Source: WormBase
  • response to oxidative stress Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.137. 1045.
ReactomeiREACT_276818. PI3K Cascade.
REACT_279166. PIP3 activates AKT signaling.
REACT_287989. Synthesis of PIPs at the plasma membrane.
REACT_291413. GAB1 signalosome.
REACT_299387. Nephrin interactions.
REACT_327367. PI3K events in ERBB2 signaling.
REACT_329929. Regulation of signaling by CBL.
REACT_335982. GPVI-mediated activation cascade.
REACT_347106. PI3K events in ERBB4 signaling.
REACT_348981. G alpha (12/13) signalling events.
REACT_352961. G alpha (q) signalling events.
REACT_360255. PI-3K cascade:FGFR3.
REACT_360528. PI-3K cascade:FGFR1.
REACT_360622. PI-3K cascade:FGFR4.
REACT_361511. PI-3K cascade:FGFR2.
SignaLinkiQ94125.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 3-kinase age-1 (EC:2.7.1.137)
Short name:
PI3-kinase age-1
Short name:
PI3K age-1
Short name:
PtdIns-3-kinase age-1
Alternative name(s):
Aging alteration protein 1
Gene namesi
Name:age-1
ORF Names:B0334.8
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiB0334.8; CE43431; WBGene00000090; age-1.

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: GOC
  • nonmotile primary cilium Source: WormBase
  • phosphatidylinositol 3-kinase complex Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi862 – 8621S → N in age1(MG109). 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11821182Phosphatidylinositol 3-kinase age-1PRO_0000088807Add
BLAST

Proteomic databases

PaxDbiQ94125.
PRIDEiQ94125.

Expressioni

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi6239.B0334.8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini74 – 174101PI3K-ABDPROSITE-ProRule annotationAdd
BLAST
Domaini266 – 35893PI3K-RBDPROSITE-ProRule annotationAdd
BLAST
Domaini425 – 577153C2 PI3K-typePROSITE-ProRule annotationAdd
BLAST
Domaini601 – 788188PIK helicalPROSITE-ProRule annotationAdd
BLAST
Domaini885 – 1182298PI3K/PI4KPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PI3/PI4-kinase family.PROSITE-ProRule annotation
Contains 1 C2 PI3K-type domain.PROSITE-ProRule annotation
Contains 1 PI3K-ABD domain.PROSITE-ProRule annotation
Contains 1 PI3K-RBD domain.PROSITE-ProRule annotation
Contains 1 PI3K/PI4K domain.PROSITE-ProRule annotation
Contains 1 PIK helical domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5032.
GeneTreeiENSGT00760000119110.
HOGENOMiHOG000175698.
InParanoidiQ94125.
KOiK00922.
OMAiIEVVPNC.
OrthoDBiEOG70CR65.
PhylomeDBiQ94125.

Family and domain databases

Gene3Di1.10.1070.11. 2 hits.
1.25.40.70. 1 hit.
2.60.40.150. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR003113. PI3K_adapt-bd_dom.
IPR002420. PI3K_C2_dom.
IPR000341. PI3K_Ras-bd_dom.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 1 hit.
PfamiPF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF02192. PI3K_p85B. 1 hit.
PF00794. PI3K_rbd. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00142. PI3K_C2. 1 hit.
SM00143. PI3K_p85B. 1 hit.
SM00144. PI3K_rbd. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51544. PI3K_ABD. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51546. PI3K_RBD. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q94125-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMGRSPSTT FRSRTGSHGA RDLIAGHGRN SRRISQMHVN ILHPQLQTMV
60 70 80 90 100
EQWQMRERPS LETENGKGSL LLENEGVADI ITMCPFGEVI SVVFPWFLAN
110 120 130 140 150
VRTSLEIKLS DFKHQLFELI APMKWGTYSV KPQDYVFRQL NNFGEIEVIF
160 170 180 190 200
NDDQPLSKLE LHGTFPMLFL YQPDGINRDK ELMSDISHCL GYSLDKLEES
210 220 230 240 250
LDEELRQFRA SLWARTKKTC LTRGLEGTSH YAFPEEQYLC VGESCPKDLE
260 270 280 290 300
SKVKAAKLSY QMFWRKRKAE INGVCEKMMK IQIEFNPNET PKSLLHTFLY
310 320 330 340 350
EMRKLDVYDT DDPADEGWFL QLAGRTTFVT NPDVKLTSYD GVRSELESYR
360 370 380 390 400
CPGFVVRRQS LVLKDYCRPK PLYEPHYVRA HERKLALDVL SVSIDSTPKQ
410 420 430 440 450
SKNSDMVMTD FRPTASLKQV SLWDLDANLM IRPVNISGFD FPADVDMYVR
460 470 480 490 500
IEFSVYVGTL TLASKSTTKV NAQFAKWNKE MYTFDLYMKD MPPSAVLSIR
510 520 530 540 550
VLYGKVKLKS EEFEVGWVNM SLTDWRDELR QGQFLFHLWA PEPTANRSRI
560 570 580 590 600
GENGARIGTN AAVTIEISSY GGRVRMPSQG QYTYLVKHRS TWTETLNIMG
610 620 630 640 650
DDYESCIRDP GYKKLQMLVK KHESGIVLEE DEQRHVWMWR RYIQKQEPDL
660 670 680 690 700
LIVLSELAFV WTDRENFSEL YVMLEKWKPP SVAAALTLLG KRCTDRVIRK
710 720 730 740 750
FAVEKLNEQL SPVTFHLFIL PLIQALKYEP RAQSEVGMML LTRALCDYRI
760 770 780 790 800
GHRLFWLLRA EIARLRDCDL KSEEYRRISL LMEAYLRGNE EHIKIITRQV
810 820 830 840 850
DMVDELTRIS TLVKGMPKDV ATMKLRDELR SISHKMENMD SPLDPVYKLG
860 870 880 890 900
EMIIDKAIVL GSAKRPLMLH WKNKNPKSDL HLPFCAMIFK NGDDLRQDML
910 920 930 940 950
VLQVLEVMDN IWKAANIDCC LNPYAVLPMG EMIGIIEVVP NCKTIFEIQV
960 970 980 990 1000
GTGFMNTAVR SIDPSFMNKW IRKQCGIEDE KKKSKKDSTK NPIEKKIDNT
1010 1020 1030 1040 1050
QAMKKYFESV DRFLYSCVGY SVATYIMGIK DRHSDNLMLT EDGKYFHIDF
1060 1070 1080 1090 1100
GHILGHGKTK LGIQRDRQPF ILTEHFMTVI RSGKSVDGNS HELQKFKTLC
1110 1120 1130 1140 1150
VEAYEVMWNN RDLFVSLFTL MLGMELPELS TKADLDHLKK TLFCNGESKE
1160 1170 1180
EARKFFAGIY EEAFNGSWST KTNWLFHAVK HY
Length:1,182
Mass (Da):136,873
Last modified:September 22, 2009 - v6
Checksum:iC82DA6F98A383EAC
GO

Sequence cautioni

The sequence AAC47459.1 differs from that shown. Reason: Frameshift at position 32. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1046 – 10461F → V in AAC47459 (PubMed:8700226).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z66519, AL110499 Genomic DNA. Translation: CAA91377.3.
U56101 mRNA. Translation: AAC47459.1. Frameshift.
PIRiB88318.
S71792.
RefSeqiNP_496462.2. NM_064061.4.
UniGeneiCel.18292.

Genome annotation databases

EnsemblMetazoaiB0334.8; B0334.8; WBGene00000090.
GeneIDi174762.
KEGGicel:CELE_B0334.8.
UCSCiB0334.8. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z66519, AL110499 Genomic DNA. Translation: CAA91377.3.
U56101 mRNA. Translation: AAC47459.1. Frameshift.
PIRiB88318.
S71792.
RefSeqiNP_496462.2. NM_064061.4.
UniGeneiCel.18292.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.B0334.8.

Proteomic databases

PaxDbiQ94125.
PRIDEiQ94125.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiB0334.8; B0334.8; WBGene00000090.
GeneIDi174762.
KEGGicel:CELE_B0334.8.
UCSCiB0334.8. c. elegans.

Organism-specific databases

CTDi174762.
WormBaseiB0334.8; CE43431; WBGene00000090; age-1.

Phylogenomic databases

eggNOGiCOG5032.
GeneTreeiENSGT00760000119110.
HOGENOMiHOG000175698.
InParanoidiQ94125.
KOiK00922.
OMAiIEVVPNC.
OrthoDBiEOG70CR65.
PhylomeDBiQ94125.

Enzyme and pathway databases

BRENDAi2.7.1.137. 1045.
ReactomeiREACT_276818. PI3K Cascade.
REACT_279166. PIP3 activates AKT signaling.
REACT_287989. Synthesis of PIPs at the plasma membrane.
REACT_291413. GAB1 signalosome.
REACT_299387. Nephrin interactions.
REACT_327367. PI3K events in ERBB2 signaling.
REACT_329929. Regulation of signaling by CBL.
REACT_335982. GPVI-mediated activation cascade.
REACT_347106. PI3K events in ERBB4 signaling.
REACT_348981. G alpha (12/13) signalling events.
REACT_352961. G alpha (q) signalling events.
REACT_360255. PI-3K cascade:FGFR3.
REACT_360528. PI-3K cascade:FGFR1.
REACT_360622. PI-3K cascade:FGFR4.
REACT_361511. PI-3K cascade:FGFR2.
SignaLinkiQ94125.

Miscellaneous databases

PROiQ94125.

Family and domain databases

Gene3Di1.10.1070.11. 2 hits.
1.25.40.70. 1 hit.
2.60.40.150. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR003113. PI3K_adapt-bd_dom.
IPR002420. PI3K_C2_dom.
IPR000341. PI3K_Ras-bd_dom.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 1 hit.
PfamiPF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF02192. PI3K_p85B. 1 hit.
PF00794. PI3K_rbd. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00142. PI3K_C2. 1 hit.
SM00143. PI3K_p85B. 1 hit.
SM00144. PI3K_rbd. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51544. PI3K_ABD. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51546. PI3K_RBD. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "A phosphatidylinositol-3-OH kinase family member regulating longevity and diapause in Caenorhabditis elegans."
    Morris J.Z., Tissenbaum H.A., Ruvkun G.
    Nature 382:536-539(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 16-1182, FUNCTION, DEVELOPMENTAL STAGE, MUTAGENESIS OF SER-862.
    Strain: Bristol N2.
  3. Morris J.Z., Tissenbaum H.A., Ruvkun G.
    Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  4. "Paralysis and killing of Caenorhabditis elegans by enteropathogenic Escherichia coli requires the bacterial tryptophanase gene."
    Anyanful A., Dolan-Livengood J.M., Lewis T., Sheth S., Dezalia M.N., Sherman M.A., Kalman L.V., Benian G.M., Kalman D.
    Mol. Microbiol. 57:988-1007(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiAGE1_CAEEL
AccessioniPrimary (citable) accession number: Q94125
Secondary accession number(s): Q17482
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 22, 2009
Last modified: June 24, 2015
This is version 129 of the entry and version 6 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.