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Q94125 (AGE1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphatidylinositol 3-kinase age-1

Short name=PI3-kinase age-1
Short name=PI3K age-1
Short name=PtdIns-3-kinase age-1
EC=2.7.1.137
Alternative name(s):
Aging alteration protein 1
Gene names
Name:age-1
ORF Names:B0334.8
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length1182 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Phosphatidylinositol 3-kinase homolog that regulates longevity and diapause. Could function in the development or neuroendocrine signaling of the dauer pathway. Ref.2

Catalytic activity

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.

Developmental stage

Expressed both maternally and zygotically. Ref.2

Sequence similarities

Belongs to the PI3/PI4-kinase family.

Contains 1 C2 PI3K-type domain.

Contains 1 PI3K-ABD domain.

Contains 1 PI3K-RBD domain.

Contains 1 PI3K/PI4K domain.

Contains 1 PIK helical domain.

Sequence caution

The sequence AAC47459.1 differs from that shown. Reason: Frameshift at position 32.

Ontologies

Keywords
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaging

Inferred from mutant phenotype Ref.2. Source: UniProtKB

dauer entry

Inferred from mutant phenotype PubMed 9504918. Source: WormBase

dauer larval development

Inferred from mutant phenotype Ref.2. Source: UniProtKB

determination of adult lifespan

Inferred from mutant phenotype PubMed 9504918. Source: WormBase

embryo development ending in birth or egg hatching

Inferred from mutant phenotype PubMed 9504918. Source: WormBase

learning or memory

Inferred from mutant phenotype PubMed 16950159. Source: WormBase

phosphatidylinositol-3-phosphate biosynthetic process

Inferred from Biological aspect of Ancestor. Source: GOC

phosphatidylinositol-mediated signaling

Inferred from electronic annotation. Source: InterPro

regulation of chemotaxis

Inferred from mutant phenotype PubMed 16950159. Source: WormBase

reproduction

Inferred from mutant phenotype PubMed 9504918. Source: WormBase

response to cadmium ion

Inferred from mutant phenotype PubMed 18828672. Source: WormBase

response to oxidative stress

Inferred from mutant phenotype PubMed 18828672. Source: WormBase

   Cellular_componentphosphatidylinositol 3-kinase complex

Non-traceable author statement Ref.2. Source: UniProtKB

plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Molecular_function1-phosphatidylinositol-3-kinase activity

Non-traceable author statement Ref.2. Source: UniProtKB

1-phosphatidylinositol-4-phosphate 3-kinase activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

ATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphatidylinositol-4,5-bisphosphate 3-kinase activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11821182Phosphatidylinositol 3-kinase age-1
PRO_0000088807

Regions

Domain74 – 174101PI3K-ABD
Domain266 – 35893PI3K-RBD
Domain425 – 577153C2 PI3K-type
Domain601 – 788188PIK helical
Domain885 – 1182298PI3K/PI4K

Experimental info

Mutagenesis8621S → N in age1(MG109). Ref.2
Sequence conflict10461F → V in AAC47459. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q94125 [UniParc].

Last modified September 22, 2009. Version 6.
Checksum: C82DA6F98A383EAC

FASTA1,182136,873
        10         20         30         40         50         60 
MSMGRSPSTT FRSRTGSHGA RDLIAGHGRN SRRISQMHVN ILHPQLQTMV EQWQMRERPS 

        70         80         90        100        110        120 
LETENGKGSL LLENEGVADI ITMCPFGEVI SVVFPWFLAN VRTSLEIKLS DFKHQLFELI 

       130        140        150        160        170        180 
APMKWGTYSV KPQDYVFRQL NNFGEIEVIF NDDQPLSKLE LHGTFPMLFL YQPDGINRDK 

       190        200        210        220        230        240 
ELMSDISHCL GYSLDKLEES LDEELRQFRA SLWARTKKTC LTRGLEGTSH YAFPEEQYLC 

       250        260        270        280        290        300 
VGESCPKDLE SKVKAAKLSY QMFWRKRKAE INGVCEKMMK IQIEFNPNET PKSLLHTFLY 

       310        320        330        340        350        360 
EMRKLDVYDT DDPADEGWFL QLAGRTTFVT NPDVKLTSYD GVRSELESYR CPGFVVRRQS 

       370        380        390        400        410        420 
LVLKDYCRPK PLYEPHYVRA HERKLALDVL SVSIDSTPKQ SKNSDMVMTD FRPTASLKQV 

       430        440        450        460        470        480 
SLWDLDANLM IRPVNISGFD FPADVDMYVR IEFSVYVGTL TLASKSTTKV NAQFAKWNKE 

       490        500        510        520        530        540 
MYTFDLYMKD MPPSAVLSIR VLYGKVKLKS EEFEVGWVNM SLTDWRDELR QGQFLFHLWA 

       550        560        570        580        590        600 
PEPTANRSRI GENGARIGTN AAVTIEISSY GGRVRMPSQG QYTYLVKHRS TWTETLNIMG 

       610        620        630        640        650        660 
DDYESCIRDP GYKKLQMLVK KHESGIVLEE DEQRHVWMWR RYIQKQEPDL LIVLSELAFV 

       670        680        690        700        710        720 
WTDRENFSEL YVMLEKWKPP SVAAALTLLG KRCTDRVIRK FAVEKLNEQL SPVTFHLFIL 

       730        740        750        760        770        780 
PLIQALKYEP RAQSEVGMML LTRALCDYRI GHRLFWLLRA EIARLRDCDL KSEEYRRISL 

       790        800        810        820        830        840 
LMEAYLRGNE EHIKIITRQV DMVDELTRIS TLVKGMPKDV ATMKLRDELR SISHKMENMD 

       850        860        870        880        890        900 
SPLDPVYKLG EMIIDKAIVL GSAKRPLMLH WKNKNPKSDL HLPFCAMIFK NGDDLRQDML 

       910        920        930        940        950        960 
VLQVLEVMDN IWKAANIDCC LNPYAVLPMG EMIGIIEVVP NCKTIFEIQV GTGFMNTAVR 

       970        980        990       1000       1010       1020 
SIDPSFMNKW IRKQCGIEDE KKKSKKDSTK NPIEKKIDNT QAMKKYFESV DRFLYSCVGY 

      1030       1040       1050       1060       1070       1080 
SVATYIMGIK DRHSDNLMLT EDGKYFHIDF GHILGHGKTK LGIQRDRQPF ILTEHFMTVI 

      1090       1100       1110       1120       1130       1140 
RSGKSVDGNS HELQKFKTLC VEAYEVMWNN RDLFVSLFTL MLGMELPELS TKADLDHLKK 

      1150       1160       1170       1180 
TLFCNGESKE EARKFFAGIY EEAFNGSWST KTNWLFHAVK HY 

« Hide

References

« Hide 'large scale' references
[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[2]"A phosphatidylinositol-3-OH kinase family member regulating longevity and diapause in Caenorhabditis elegans."
Morris J.Z., Tissenbaum H.A., Ruvkun G.
Nature 382:536-539(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 16-1182, FUNCTION, DEVELOPMENTAL STAGE, MUTAGENESIS OF SER-862.
Strain: Bristol N2.
[3]Morris J.Z., Tissenbaum H.A., Ruvkun G.
Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z66519, AL110499 Genomic DNA. Translation: CAA91377.3.
U56101 mRNA. Translation: AAC47459.1. Frameshift.
PIRB88318.
S71792.
RefSeqNP_496462.2. NM_064061.4.
UniGeneCel.18292.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ94125.
PRIDEQ94125.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaB0334.8; B0334.8; WBGene00000090.
GeneID174762.
KEGGcel:CELE_B0334.8.
UCSCB0334.8. c. elegans.

Organism-specific databases

CTD174762.
WormBaseB0334.8; CE43431; WBGene00000090; age-1.

Phylogenomic databases

eggNOGCOG5032.
GeneTreeENSGT00620000087742.
HOGENOMHOG000175698.
OMACERAFLV.
OrthoDBEOG70CR65.
PhylomeDBQ94125.

Enzyme and pathway databases

BRENDA2.7.1.137. 1045.
SignaLinkQ94125.

Family and domain databases

Gene3D1.10.1070.11. 2 hits.
1.25.40.70. 1 hit.
2.60.40.150. 1 hit.
InterProIPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR003113. PI3K_adapt-bd_dom.
IPR002420. PI3K_C2_dom.
IPR000341. PI3K_Ras-bd_dom.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERPTHR10048. PTHR10048. 1 hit.
PfamPF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF02192. PI3K_p85B. 1 hit.
PF00794. PI3K_rbd. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
SMARTSM00239. C2. 1 hit.
SM00142. PI3K_C2. 1 hit.
SM00143. PI3K_p85B. 1 hit.
SM00144. PI3K_rbd. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51544. PI3K_ABD. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51546. PI3K_RBD. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAGE1_CAEEL
AccessionPrimary (citable) accession number: Q94125
Secondary accession number(s): Q17482
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 22, 2009
Last modified: June 11, 2014
This is version 119 of the entry and version 6 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase