Q94125 (AGE1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylinositol 3-kinase age-1
Short name=PI3-kinase age-1
Short name=PI3K age-1
Short name=PtdIns-3-kinase age-1
EC=2.7.1.137

Alternative name(s):
Aging alteration protein 1

Gene names

Name:	age-1
ORF Names:	B0334.8

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	1182 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phosphatidylinositol 3-kinase homolog that regulates longevity and diapause. Could function in the development or neuroendocrine signaling of the dauer pathway. Ref.2
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.
Developmental stage	Expressed both maternally and zygotically. Ref.2
Sequence similarities	Belongs to the PI3/PI4-kinase family. Contains 1 C2 PI3K-type domain. Contains 1 PI3K-ABD domain. Contains 1 PI3K-RBD domain. Contains 1 PI3K/PI4K domain. Contains 1 PIK helical domain.
Sequence caution	The sequence AAC47459.1 differs from that shown. Reason: Frameshift at position 32.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dauer entry Inferred from mutant phenotype PubMed 9504918. Source: WormBase determination of adult lifespan Inferred from mutant phenotype PubMed 9504918. Source: WormBase embryo development ending in birth or egg hatching Inferred from mutant phenotype PubMed 9504918. Source: WormBase learning or memory Inferred from mutant phenotype PubMed 16950159. Source: WormBase phosphatidylinositol-mediated signaling Inferred from electronic annotation. Source: InterPro regulation of chemotaxis Inferred from mutant phenotype PubMed 16950159. Source: WormBase reproduction Inferred from mutant phenotype PubMed 9504918. Source: WormBase response to cadmium ion Inferred from mutant phenotype PubMed 18828672. Source: WormBase response to oxidative stress Inferred from mutant phenotype PubMed 18828672. Source: WormBase
Cellular_component	phosphatidylinositol 3-kinase complex Non-traceable author statement Ref.2. Source: UniProtKB plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	1-phosphatidylinositol-3-kinase activity Non-traceable author statement Ref.2. Source: UniProtKB 1-phosphatidylinositol-4-phosphate 3-kinase activity Inferred from Biological aspect of Ancestor. Source: RefGenome ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylinositol-4,5-bisphosphate 3-kinase activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1182	1182	Phosphatidylinositol 3-kinase age-1		PRO_0000088807
Regions
	Domain	74 – 174	101	PI3K-ABD
	Domain	266 – 358	93	PI3K-RBD
	Domain	425 – 577	153	C2 PI3K-type
	Domain	601 – 788	188	PIK helical
	Domain	885 – 1182	298	PI3K/PI4K
Experimental info
	Mutagenesis	862	1	S → N in age1(MG109). Ref.2
	Sequence conflict	1046	1	F → V in AAC47459. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q94125 [UniParc]. Last modified September 22, 2009. Version 6. Checksum: C82DA6F98A383EAC	FASTA	1,182	136,873
	10 20 30 40 50 60 MSMGRSPSTT FRSRTGSHGA RDLIAGHGRN SRRISQMHVN ILHPQLQTMV EQWQMRERPS 70 80 90 100 110 120 LETENGKGSL LLENEGVADI ITMCPFGEVI SVVFPWFLAN VRTSLEIKLS DFKHQLFELI 130 140 150 160 170 180 APMKWGTYSV KPQDYVFRQL NNFGEIEVIF NDDQPLSKLE LHGTFPMLFL YQPDGINRDK 190 200 210 220 230 240 ELMSDISHCL GYSLDKLEES LDEELRQFRA SLWARTKKTC LTRGLEGTSH YAFPEEQYLC 250 260 270 280 290 300 VGESCPKDLE SKVKAAKLSY QMFWRKRKAE INGVCEKMMK IQIEFNPNET PKSLLHTFLY 310 320 330 340 350 360 EMRKLDVYDT DDPADEGWFL QLAGRTTFVT NPDVKLTSYD GVRSELESYR CPGFVVRRQS 370 380 390 400 410 420 LVLKDYCRPK PLYEPHYVRA HERKLALDVL SVSIDSTPKQ SKNSDMVMTD FRPTASLKQV 430 440 450 460 470 480 SLWDLDANLM IRPVNISGFD FPADVDMYVR IEFSVYVGTL TLASKSTTKV NAQFAKWNKE 490 500 510 520 530 540 MYTFDLYMKD MPPSAVLSIR VLYGKVKLKS EEFEVGWVNM SLTDWRDELR QGQFLFHLWA 550 560 570 580 590 600 PEPTANRSRI GENGARIGTN AAVTIEISSY GGRVRMPSQG QYTYLVKHRS TWTETLNIMG 610 620 630 640 650 660 DDYESCIRDP GYKKLQMLVK KHESGIVLEE DEQRHVWMWR RYIQKQEPDL LIVLSELAFV 670 680 690 700 710 720 WTDRENFSEL YVMLEKWKPP SVAAALTLLG KRCTDRVIRK FAVEKLNEQL SPVTFHLFIL 730 740 750 760 770 780 PLIQALKYEP RAQSEVGMML LTRALCDYRI GHRLFWLLRA EIARLRDCDL KSEEYRRISL 790 800 810 820 830 840 LMEAYLRGNE EHIKIITRQV DMVDELTRIS TLVKGMPKDV ATMKLRDELR SISHKMENMD 850 860 870 880 890 900 SPLDPVYKLG EMIIDKAIVL GSAKRPLMLH WKNKNPKSDL HLPFCAMIFK NGDDLRQDML 910 920 930 940 950 960 VLQVLEVMDN IWKAANIDCC LNPYAVLPMG EMIGIIEVVP NCKTIFEIQV GTGFMNTAVR 970 980 990 1000 1010 1020 SIDPSFMNKW IRKQCGIEDE KKKSKKDSTK NPIEKKIDNT QAMKKYFESV DRFLYSCVGY 1030 1040 1050 1060 1070 1080 SVATYIMGIK DRHSDNLMLT EDGKYFHIDF GHILGHGKTK LGIQRDRQPF ILTEHFMTVI 1090 1100 1110 1120 1130 1140 RSGKSVDGNS HELQKFKTLC VEAYEVMWNN RDLFVSLFTL MLGMELPELS TKADLDHLKK 1150 1160 1170 1180 TLFCNGESKE EARKFFAGIY EEAFNGSWST KTNWLFHAVK HY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"A phosphatidylinositol-3-OH kinase family member regulating longevity and diapause in Caenorhabditis elegans." Morris J.Z., Tissenbaum H.A., Ruvkun G. Nature 382:536-539(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 16-1182, FUNCTION, DEVELOPMENTAL STAGE, MUTAGENESIS OF SER-862. Strain: Bristol N2.
[3]	Morris J.Z., Tissenbaum H.A., Ruvkun G. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z66519, AL110499 Genomic DNA. Translation: CAA91377.3. U56101 mRNA. Translation: AAC47459.1. Frameshift.
PIR	B88318. S71792.
RefSeq	NP_496462.2. NM_064061.4.
3D structure databases
ProteinModelPortal	Q94125.
SMR	Q94125. Positions 434-1179.
ModBase	Search...
Proteomic databases
PaxDb	Q94125.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	B0334.8; B0334.8; B0334.8.
GeneID	174762.
KEGG	cel:CELE_B0334.8.
UCSC	B0334.8. c. elegans.
Organism-specific databases
CTD	174762.
WormBase	B0334.8; CE43431; WBGene00000090; age-1.
Phylogenomic databases
eggNOG	COG5032.
GeneTree	ENSGT00620000087805.
HOGENOM	HOG000175698.
OMA	CERAFLV.
Enzyme and pathway databases
BRENDA	2.7.1.137. 1045.
Family and domain databases
Gene3D	1.10.1070.11. 2 hits. 1.25.40.70. 1 hit.
InterPro	IPR016024. ARM-type_fold. IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR011009. Kinase-like_dom. IPR000403. PI3/4_kinase_cat_dom. IPR018936. PI3/4_kinase_CS. IPR003113. PI3K_adapt-bd_dom. IPR002420. PI3K_C2_dom. IPR000341. PI3K_Ras-bd_dom. IPR015433. PI_Kinase. IPR001263. PInositide-3_kin_accessory_dom. [Graphical view]
PANTHER	PTHR10048. PTHR10048. 1 hit.
Pfam	PF00454. PI3_PI4_kinase. 1 hit. PF00792. PI3K_C2. 1 hit. PF02192. PI3K_p85B. 1 hit. PF00794. PI3K_rbd. 1 hit. PF00613. PI3Ka. 1 hit. [Graphical view]
SMART	SM00239. C2. 1 hit. SM00142. PI3K_C2. 1 hit. SM00143. PI3K_p85B. 1 hit. SM00144. PI3K_rbd. 1 hit. SM00145. PI3Ka. 1 hit. SM00146. PI3Kc. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit. SSF49562. C2_CaLB. 1 hit. SSF56112. Kinase_like. 1 hit.
PROSITE	PS50004. C2. False negative. PS00915. PI3_4_KINASE_1. 1 hit. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. PS51544. PI3K_ABD. 1 hit. PS51547. PI3K_C2. 1 hit. PS51546. PI3K_RBD. 1 hit. PS51545. PIK_HELICAL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGE1_CAEEL

Accession

Primary (citable) accession number: Q94125
Secondary accession number(s): Q17482

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	September 22, 2009
Last modified:	May 1, 2013
This is version 111 of the entry and version 6 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project