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Q940U6 (FLU_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein FLUORESCENT IN BLUE LIGHT, chloroplastic
Gene names
Name:FLU
Ordered Locus Names:At3g14110
ORF Names:MAG2.7
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length316 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Negative regulator of tetrapyrrole biosynthesis (including chlorophyll) in chloroplasts, probably via HEMA1 repression. Inhibits especially the magnesium ion Mg2+ branch of tetrapyrrole biosynthesis, but independently of heme. Ref.6 Ref.8 Ref.11 Ref.12

Subunit structure

Part of the FLU-containing chloroplast membrane complex composed of FLU, CRD1, PORB, PORC, CHLP and HEMA1. Interacts with HEMA1 (via C-terminus) only in the absence of light. No interaction with HEMA2. Ref.11 Ref.15

Subcellular location

Plastidchloroplast membrane; Single-pass membrane protein. Plastidchloroplast thylakoid membrane; Single-pass membrane protein Ref.6 Ref.7 Ref.9 Ref.10 Ref.13 Ref.14 Ref.15.

Disruption phenotype

Loss of ability to restrict the accumulation of protochloro-phyllide (Pchlide) and delta-amin-olevulinic acid (ALA) in the dark, leading to a strong Pchlide fluorescence in etiolated mutant seedlings exposed to blue light. Rapid bleaching and death of plants transferred from the dark to the light, mediated by singlet oxygen production and enzymatic peroxidation of linolenic acid. Ref.6 Ref.8 Ref.11 Ref.12

Sequence similarities

Contains 3 TPR repeats.

Sequence caution

The sequence BAB02975.1 differs from that shown. Reason: Erroneous gene model prediction.

Binary interactions

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q940U6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2626Chloroplast Potential
Chain27 – 316290Protein FLUORESCENT IN BLUE LIGHT, chloroplastic
PRO_0000407552

Regions

Transmembrane125 – 14521Helical; Potential
Repeat203 – 23634TPR 1
Repeat243 – 27634TPR 2
Repeat283 – 31634TPR 3
Coiled coil144 – 17532 Potential

Experimental info

Mutagenesis1461A → V in flu1-4; rapid bleaching and death when transferred from the dark to the light, accumulation of Pchlide and ALA. Ref.6
Mutagenesis2621A → V in flu1-1; rapid bleaching and death when transferred from the dark to the light, accumulation of Pchlide and ALA, and impaired HEMA1 interaction. Ref.6 Ref.11
Sequence conflict971M → L in BAH20437. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 8A647B74684B6514

FASTA31634,587
        10         20         30         40         50         60 
MAALIRCCSS FSHTSGGQPP PRDKSRAPEI GKFATSIGYS VVRKPGDHPP FSKIIHSSSQ 

        70         80         90        100        110        120 
PKERQGKGIL QTPFASVGSL DKFSAFEGIG RLKLPVMAVL LTNSLQMATP LEALAAEICE 

       130        140        150        160        170        180 
PESSMFSMPI LLLVALIGAT VGGLLARQRK GELQRLNEQL RQINAALRRQ AKIESYAPSL 

       190        200        210        220        230        240 
SYAPVGARIP DSEIIVEPKK QELISKLKTG KTFLRNQEPE KAYTEFKIAL ELAQSLKDPT 

       250        260        270        280        290        300 
EEKKAARGLG ASLQRQGKYR EAIQYHSMVL AISKRESEDS GITEAYGAIA DCYTELGDLE 

       310 
KAGKFYDTYI ARLETD 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[4]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 97-316 (ISOFORM 1).
Strain: cv. Columbia.
Tissue: Flower and Silique.
[6]"FLU: a negative regulator of chlorophyll biosynthesis in Arabidopsis thaliana."
Meskauskiene R., Nater M., Goslings D., Kessler F., op den Camp R.G.L., Apel K.
Proc. Natl. Acad. Sci. U.S.A. 98:12826-12831(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF ALA-146 AND ALA-262, SUBCELLULAR LOCATION.
Strain: cv. Landsberg erecta.
[7]"Proteomic study of the Arabidopsis thaliana chloroplastic envelope membrane utilizing alternatives to traditional two-dimensional electrophoresis."
Froehlich J.E., Wilkerson C.G., Ray W.K., McAndrew R.S., Osteryoung K.W., Gage D.A., Phinney B.S.
J. Proteome Res. 2:413-425(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Rapid induction of distinct stress responses after the release of singlet oxygen in Arabidopsis."
op den Camp R.G.L., Przybyla D., Ochsenbein C., Laloi C., Kim C., Danon A., Wagner D., Hideg E., Goebel C., Feussner I., Nater M., Apel K.
Plant Cell 15:2320-2332(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[9]"The Arabidopsis thaliana chloroplast proteome reveals pathway abundance and novel protein functions."
Kleffmann T., Russenberger D., von Zychlinski A., Christopher W., Sjoelander K., Gruissem W., Baginsky S.
Curr. Biol. 14:354-362(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]"New functions of the thylakoid membrane proteome of Arabidopsis thaliana revealed by a simple, fast, and versatile fractionation strategy."
Peltier J.-B., Ytterberg A.J., Sun Q., van Wijk K.J.
J. Biol. Chem. 279:49367-49383(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[11]"Concurrent interactions of heme and FLU with Glu tRNA reductase (HEMA1), the target of metabolic feedback inhibition of tetrapyrrole biosynthesis, in dark- and light-grown Arabidopsis plants."
Goslings D., Meskauskiene R., Kim C., Lee K.P., Nater M., Apel K.
Plant J. 40:957-967(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF ALA-262, INTERACTION WITH HEMA1 AND HEMA2.
Strain: cv. Landsberg erecta.
[12]"Enzymatic, but not non-enzymatic, 1O2-mediated peroxidation of polyunsaturated fatty acids forms part of the EXECUTER1-dependent stress response program in the flu mutant of Arabidopsis thaliana."
Przybyla D., Goebel C., Imboden A., Hamberg M., Feussner I., Apel K.
Plant J. 54:236-248(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
Strain: cv. Columbia.
[13]"Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[14]"AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[15]"FLU, a negative feedback regulator of tetrapyrrole biosynthesis, is physically linked to the final steps of the Mg(++)-branch of this pathway."
Kauss D., Bischof S., Steiner S., Apel K., Meskauskiene R.
FEBS Lett. 586:211-216(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN THE FLU-CONTAINING CHLOROPLAST MEMBRANE COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP000600 Genomic DNA. Translation: BAB02975.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE75470.1.
AF446890 mRNA. Translation: AAL38623.1.
AY052677 mRNA. Translation: AAK96581.1.
AY087529 mRNA. Translation: AAM65071.1.
AK317782 mRNA. Translation: BAH20437.1.
RefSeqNP_566478.1. NM_112267.3. [Q940U6-1]
UniGeneAt.22594.
At.70203.

3D structure databases

ProteinModelPortalQ940U6.
SMRQ940U6. Positions 210-312.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid5962. 1 interaction.
IntActQ940U6. 5 interactions.
MINTMINT-8300912.
STRING3702.AT3G14110.1-P.

Proteomic databases

PaxDbQ940U6.
PRIDEQ940U6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G14110.1; AT3G14110.1; AT3G14110. [Q940U6-1]
GeneID820628.
KEGGath:AT3G14110.

Organism-specific databases

TAIRAT3G14110.

Phylogenomic databases

eggNOGNOG288145.
HOGENOMHOG000239198.
InParanoidQ940U6.
PhylomeDBQ940U6.

Gene expression databases

ArrayExpressQ940U6.
GenevestigatorQ940U6.

Family and domain databases

Gene3D1.25.40.10. 1 hit.
InterProIPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical.
[Graphical view]
PROSITEPS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFLU_ARATH
AccessionPrimary (citable) accession number: Q940U6
Secondary accession number(s): B9DI70, Q9LJH7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: December 1, 2001
Last modified: June 11, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names