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Protein

Protein FLUORESCENT IN BLUE LIGHT, chloroplastic

Gene

FLU

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negative regulator of tetrapyrrole biosynthesis (including chlorophyll) in chloroplasts, probably via HEMA1 repression. Inhibits especially the magnesium ion Mg2+ branch of tetrapyrrole biosynthesis, but independently of heme.4 Publications

Names & Taxonomyi

Protein namesi
Recommended name:
Protein FLUORESCENT IN BLUE LIGHT, chloroplastic
Gene namesi
Name:FLU
Ordered Locus Names:At3g14110
ORF Names:MAG2.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G14110.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei125 – 145HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

Pathology & Biotechi

Disruption phenotypei

Loss of ability to restrict the accumulation of protochloro-phyllide (Pchlide) and delta-amin-olevulinic acid (ALA) in the dark, leading to a strong Pchlide fluorescence in etiolated mutant seedlings exposed to blue light. Rapid bleaching and death of plants transferred from the dark to the light, mediated by singlet oxygen production and enzymatic peroxidation of linolenic acid.4 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi146A → V in flu1-4; rapid bleaching and death when transferred from the dark to the light, accumulation of Pchlide and ALA. 1 Publication1
Mutagenesisi262A → V in flu1-1; rapid bleaching and death when transferred from the dark to the light, accumulation of Pchlide and ALA, and impaired HEMA1 interaction. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 26ChloroplastSequence analysisAdd BLAST26
ChainiPRO_000040755227 – 316Protein FLUORESCENT IN BLUE LIGHT, chloroplasticAdd BLAST290

Proteomic databases

PaxDbiQ940U6.

Expressioni

Gene expression databases

ExpressionAtlasiQ940U6. baseline and differential.
GenevisibleiQ940U6. AT.

Interactioni

Subunit structurei

Part of the FLU-containing chloroplast membrane complex composed of FLU, CRD1, PORB, PORC, CHLP and HEMA1. Interacts with HEMA1 (via C-terminus) only in the absence of light. No interaction with HEMA2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CRD1Q9M5915EBI-2319882,EBI-7632098
HEMA1P428043EBI-2319882,EBI-2319900

Protein-protein interaction databases

BioGridi5962. 1 interactor.
IntActiQ940U6. 5 interactors.
MINTiMINT-8300912.
STRINGi3702.AT3G14110.3.

Structurei

Secondary structure

1316
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi199 – 215Combined sources17
Helixi219 – 235Combined sources17
Helixi239 – 255Combined sources17
Helixi259 – 276Combined sources18
Helixi282 – 295Combined sources14
Helixi299 – 313Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4YVOX-ray1.45A189-316[»]
4YVQX-ray2.40C195-316[»]
5CHEX-ray3.20E/F195-316[»]
ProteinModelPortaliQ940U6.
SMRiQ940U6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati203 – 236TPR 1Add BLAST34
Repeati243 – 276TPR 2Add BLAST34
Repeati283 – 316TPR 3Add BLAST34

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili144 – 175Sequence analysisAdd BLAST32

Sequence similaritiesi

Contains 3 TPR repeats.Curated

Keywords - Domaini

Coiled coil, Repeat, TPR repeat, Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEEH. Eukaryota.
ENOG4111KCW. LUCA.
HOGENOMiHOG000239198.
InParanoidiQ940U6.
PhylomeDBiQ940U6.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
PROSITEiPS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q940U6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAALIRCCSS FSHTSGGQPP PRDKSRAPEI GKFATSIGYS VVRKPGDHPP
60 70 80 90 100
FSKIIHSSSQ PKERQGKGIL QTPFASVGSL DKFSAFEGIG RLKLPVMAVL
110 120 130 140 150
LTNSLQMATP LEALAAEICE PESSMFSMPI LLLVALIGAT VGGLLARQRK
160 170 180 190 200
GELQRLNEQL RQINAALRRQ AKIESYAPSL SYAPVGARIP DSEIIVEPKK
210 220 230 240 250
QELISKLKTG KTFLRNQEPE KAYTEFKIAL ELAQSLKDPT EEKKAARGLG
260 270 280 290 300
ASLQRQGKYR EAIQYHSMVL AISKRESEDS GITEAYGAIA DCYTELGDLE
310
KAGKFYDTYI ARLETD
Length:316
Mass (Da):34,587
Last modified:December 1, 2001 - v1
Checksum:i8A647B74684B6514
GO

Sequence cautioni

The sequence BAB02975 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti97M → L in BAH20437 (PubMed:19423640).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000600 Genomic DNA. Translation: BAB02975.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE75470.1.
AF446890 mRNA. Translation: AAL38623.1.
AY052677 mRNA. Translation: AAK96581.1.
AY087529 mRNA. Translation: AAM65071.1.
AK317782 mRNA. Translation: BAH20437.1.
RefSeqiNP_566478.1. NM_112267.4. [Q940U6-1]
UniGeneiAt.22594.
At.70203.

Genome annotation databases

EnsemblPlantsiAT3G14110.1; AT3G14110.1; AT3G14110. [Q940U6-1]
GeneIDi820628.
GrameneiAT3G14110.1; AT3G14110.1; AT3G14110.
KEGGiath:AT3G14110.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000600 Genomic DNA. Translation: BAB02975.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE75470.1.
AF446890 mRNA. Translation: AAL38623.1.
AY052677 mRNA. Translation: AAK96581.1.
AY087529 mRNA. Translation: AAM65071.1.
AK317782 mRNA. Translation: BAH20437.1.
RefSeqiNP_566478.1. NM_112267.4. [Q940U6-1]
UniGeneiAt.22594.
At.70203.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4YVOX-ray1.45A189-316[»]
4YVQX-ray2.40C195-316[»]
5CHEX-ray3.20E/F195-316[»]
ProteinModelPortaliQ940U6.
SMRiQ940U6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi5962. 1 interactor.
IntActiQ940U6. 5 interactors.
MINTiMINT-8300912.
STRINGi3702.AT3G14110.3.

Proteomic databases

PaxDbiQ940U6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G14110.1; AT3G14110.1; AT3G14110. [Q940U6-1]
GeneIDi820628.
GrameneiAT3G14110.1; AT3G14110.1; AT3G14110.
KEGGiath:AT3G14110.

Organism-specific databases

TAIRiAT3G14110.

Phylogenomic databases

eggNOGiENOG410IEEH. Eukaryota.
ENOG4111KCW. LUCA.
HOGENOMiHOG000239198.
InParanoidiQ940U6.
PhylomeDBiQ940U6.

Miscellaneous databases

PROiQ940U6.

Gene expression databases

ExpressionAtlasiQ940U6. baseline and differential.
GenevisibleiQ940U6. AT.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
PROSITEiPS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFLU_ARATH
AccessioniPrimary (citable) accession number: Q940U6
Secondary accession number(s): B9DI70, Q9LJH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.