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Q940U6

- FLU_ARATH

UniProt

Q940U6 - FLU_ARATH

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Protein

Protein FLUORESCENT IN BLUE LIGHT, chloroplastic

Gene

FLU

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Negative regulator of tetrapyrrole biosynthesis (including chlorophyll) in chloroplasts, probably via HEMA1 repression. Inhibits especially the magnesium ion Mg2+ branch of tetrapyrrole biosynthesis, but independently of heme.4 Publications

Names & Taxonomyi

Protein namesi
Recommended name:
Protein FLUORESCENT IN BLUE LIGHT, chloroplastic
Gene namesi
Name:FLU
Ordered Locus Names:At3g14110
ORF Names:MAG2.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G14110.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei125 – 14521HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. thylakoid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

Pathology & Biotechi

Disruption phenotypei

Loss of ability to restrict the accumulation of protochloro-phyllide (Pchlide) and delta-amin-olevulinic acid (ALA) in the dark, leading to a strong Pchlide fluorescence in etiolated mutant seedlings exposed to blue light. Rapid bleaching and death of plants transferred from the dark to the light, mediated by singlet oxygen production and enzymatic peroxidation of linolenic acid.4 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi146 – 1461A → V in flu1-4; rapid bleaching and death when transferred from the dark to the light, accumulation of Pchlide and ALA. 1 Publication
Mutagenesisi262 – 2621A → V in flu1-1; rapid bleaching and death when transferred from the dark to the light, accumulation of Pchlide and ALA, and impaired HEMA1 interaction. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2626ChloroplastSequence AnalysisAdd
BLAST
Chaini27 – 316290Protein FLUORESCENT IN BLUE LIGHT, chloroplasticPRO_0000407552Add
BLAST

Proteomic databases

PaxDbiQ940U6.
PRIDEiQ940U6.

Expressioni

Gene expression databases

ExpressionAtlasiQ940U6. baseline and differential.
GenevestigatoriQ940U6.

Interactioni

Subunit structurei

Part of the FLU-containing chloroplast membrane complex composed of FLU, CRD1, PORB, PORC, CHLP and HEMA1. Interacts with HEMA1 (via C-terminus) only in the absence of light. No interaction with HEMA2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CRD1Q9M5915EBI-2319882,EBI-7632098
HEMA1P428043EBI-2319882,EBI-2319900

Protein-protein interaction databases

BioGridi5962. 1 interaction.
IntActiQ940U6. 5 interactions.
MINTiMINT-8300912.
STRINGi3702.AT3G14110.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ940U6.
SMRiQ940U6. Positions 210-310.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati203 – 23634TPR 1Add
BLAST
Repeati243 – 27634TPR 2Add
BLAST
Repeati283 – 31634TPR 3Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili144 – 17532Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 3 TPR repeats.Curated

Keywords - Domaini

Coiled coil, Repeat, TPR repeat, Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG288145.
HOGENOMiHOG000239198.
InParanoidiQ940U6.
PhylomeDBiQ940U6.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PROSITEiPS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q940U6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAALIRCCSS FSHTSGGQPP PRDKSRAPEI GKFATSIGYS VVRKPGDHPP
60 70 80 90 100
FSKIIHSSSQ PKERQGKGIL QTPFASVGSL DKFSAFEGIG RLKLPVMAVL
110 120 130 140 150
LTNSLQMATP LEALAAEICE PESSMFSMPI LLLVALIGAT VGGLLARQRK
160 170 180 190 200
GELQRLNEQL RQINAALRRQ AKIESYAPSL SYAPVGARIP DSEIIVEPKK
210 220 230 240 250
QELISKLKTG KTFLRNQEPE KAYTEFKIAL ELAQSLKDPT EEKKAARGLG
260 270 280 290 300
ASLQRQGKYR EAIQYHSMVL AISKRESEDS GITEAYGAIA DCYTELGDLE
310
KAGKFYDTYI ARLETD
Length:316
Mass (Da):34,587
Last modified:December 1, 2001 - v1
Checksum:i8A647B74684B6514
GO

Sequence cautioni

The sequence BAB02975.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti97 – 971M → L in BAH20437. (PubMed:19423640)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000600 Genomic DNA. Translation: BAB02975.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE75470.1.
AF446890 mRNA. Translation: AAL38623.1.
AY052677 mRNA. Translation: AAK96581.1.
AY087529 mRNA. Translation: AAM65071.1.
AK317782 mRNA. Translation: BAH20437.1.
RefSeqiNP_566478.1. NM_112267.3. [Q940U6-1]
UniGeneiAt.22594.
At.70203.

Genome annotation databases

EnsemblPlantsiAT3G14110.1; AT3G14110.1; AT3G14110. [Q940U6-1]
GeneIDi820628.
KEGGiath:AT3G14110.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000600 Genomic DNA. Translation: BAB02975.1 . Sequence problems.
CP002686 Genomic DNA. Translation: AEE75470.1 .
AF446890 mRNA. Translation: AAL38623.1 .
AY052677 mRNA. Translation: AAK96581.1 .
AY087529 mRNA. Translation: AAM65071.1 .
AK317782 mRNA. Translation: BAH20437.1 .
RefSeqi NP_566478.1. NM_112267.3. [Q940U6-1 ]
UniGenei At.22594.
At.70203.

3D structure databases

ProteinModelPortali Q940U6.
SMRi Q940U6. Positions 210-310.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 5962. 1 interaction.
IntActi Q940U6. 5 interactions.
MINTi MINT-8300912.
STRINGi 3702.AT3G14110.1-P.

Proteomic databases

PaxDbi Q940U6.
PRIDEi Q940U6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G14110.1 ; AT3G14110.1 ; AT3G14110 . [Q940U6-1 ]
GeneIDi 820628.
KEGGi ath:AT3G14110.

Organism-specific databases

TAIRi AT3G14110.

Phylogenomic databases

eggNOGi NOG288145.
HOGENOMi HOG000239198.
InParanoidi Q940U6.
PhylomeDBi Q940U6.

Gene expression databases

ExpressionAtlasi Q940U6. baseline and differential.
Genevestigatori Q940U6.

Family and domain databases

Gene3Di 1.25.40.10. 1 hit.
InterProi IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
[Graphical view ]
PROSITEi PS50293. TPR_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 97-316 (ISOFORM 1).
    Strain: cv. Columbia.
    Tissue: Flower and Silique.
  6. "FLU: a negative regulator of chlorophyll biosynthesis in Arabidopsis thaliana."
    Meskauskiene R., Nater M., Goslings D., Kessler F., op den Camp R.G.L., Apel K.
    Proc. Natl. Acad. Sci. U.S.A. 98:12826-12831(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF ALA-146 AND ALA-262, SUBCELLULAR LOCATION.
    Strain: cv. Landsberg erecta.
  7. "Proteomic study of the Arabidopsis thaliana chloroplastic envelope membrane utilizing alternatives to traditional two-dimensional electrophoresis."
    Froehlich J.E., Wilkerson C.G., Ray W.K., McAndrew R.S., Osteryoung K.W., Gage D.A., Phinney B.S.
    J. Proteome Res. 2:413-425(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. "Rapid induction of distinct stress responses after the release of singlet oxygen in Arabidopsis."
    op den Camp R.G.L., Przybyla D., Ochsenbein C., Laloi C., Kim C., Danon A., Wagner D., Hideg E., Goebel C., Feussner I., Nater M., Apel K.
    Plant Cell 15:2320-2332(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  9. "The Arabidopsis thaliana chloroplast proteome reveals pathway abundance and novel protein functions."
    Kleffmann T., Russenberger D., von Zychlinski A., Christopher W., Sjoelander K., Gruissem W., Baginsky S.
    Curr. Biol. 14:354-362(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. "New functions of the thylakoid membrane proteome of Arabidopsis thaliana revealed by a simple, fast, and versatile fractionation strategy."
    Peltier J.-B., Ytterberg A.J., Sun Q., van Wijk K.J.
    J. Biol. Chem. 279:49367-49383(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  11. "Concurrent interactions of heme and FLU with Glu tRNA reductase (HEMA1), the target of metabolic feedback inhibition of tetrapyrrole biosynthesis, in dark- and light-grown Arabidopsis plants."
    Goslings D., Meskauskiene R., Kim C., Lee K.P., Nater M., Apel K.
    Plant J. 40:957-967(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF ALA-262, INTERACTION WITH HEMA1 AND HEMA2.
    Strain: cv. Landsberg erecta.
  12. "Enzymatic, but not non-enzymatic, 1O2-mediated peroxidation of polyunsaturated fatty acids forms part of the EXECUTER1-dependent stress response program in the flu mutant of Arabidopsis thaliana."
    Przybyla D., Goebel C., Imboden A., Hamberg M., Feussner I., Apel K.
    Plant J. 54:236-248(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: cv. Columbia.
  13. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
    Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
    PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  14. "AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
    Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
    Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  15. "FLU, a negative feedback regulator of tetrapyrrole biosynthesis, is physically linked to the final steps of the Mg(++)-branch of this pathway."
    Kauss D., Bischof S., Steiner S., Apel K., Meskauskiene R.
    FEBS Lett. 586:211-216(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN THE FLU-CONTAINING CHLOROPLAST MEMBRANE COMPLEX.

Entry informationi

Entry nameiFLU_ARATH
AccessioniPrimary (citable) accession number: Q940U6
Secondary accession number(s): B9DI70, Q9LJH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: December 1, 2001
Last modified: October 29, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3