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Q940U6

- FLU_ARATH

UniProt

Q940U6 - FLU_ARATH

Protein

Protein FLUORESCENT IN BLUE LIGHT, chloroplastic

Gene

FLU

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Negative regulator of tetrapyrrole biosynthesis (including chlorophyll) in chloroplasts, probably via HEMA1 repression. Inhibits especially the magnesium ion Mg2+ branch of tetrapyrrole biosynthesis, but independently of heme.4 Publications

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein FLUORESCENT IN BLUE LIGHT, chloroplastic
    Gene namesi
    Name:FLU
    Ordered Locus Names:At3g14110
    ORF Names:MAG2.7
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G14110.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast membrane Source: UniProtKB-SubCell
    2. chloroplast thylakoid membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Chloroplast, Membrane, Plastid, Thylakoid

    Pathology & Biotechi

    Disruption phenotypei

    Loss of ability to restrict the accumulation of protochloro-phyllide (Pchlide) and delta-amin-olevulinic acid (ALA) in the dark, leading to a strong Pchlide fluorescence in etiolated mutant seedlings exposed to blue light. Rapid bleaching and death of plants transferred from the dark to the light, mediated by singlet oxygen production and enzymatic peroxidation of linolenic acid.4 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi146 – 1461A → V in flu1-4; rapid bleaching and death when transferred from the dark to the light, accumulation of Pchlide and ALA. 1 Publication
    Mutagenesisi262 – 2621A → V in flu1-1; rapid bleaching and death when transferred from the dark to the light, accumulation of Pchlide and ALA, and impaired HEMA1 interaction. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2626ChloroplastSequence AnalysisAdd
    BLAST
    Chaini27 – 316290Protein FLUORESCENT IN BLUE LIGHT, chloroplasticPRO_0000407552Add
    BLAST

    Proteomic databases

    PaxDbiQ940U6.
    PRIDEiQ940U6.

    Expressioni

    Gene expression databases

    ArrayExpressiQ940U6.
    GenevestigatoriQ940U6.

    Interactioni

    Subunit structurei

    Part of the FLU-containing chloroplast membrane complex composed of FLU, CRD1, PORB, PORC, CHLP and HEMA1. Interacts with HEMA1 (via C-terminus) only in the absence of light. No interaction with HEMA2.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CRD1Q9M5915EBI-2319882,EBI-7632098
    HEMA1P428043EBI-2319882,EBI-2319900

    Protein-protein interaction databases

    BioGridi5962. 1 interaction.
    IntActiQ940U6. 5 interactions.
    MINTiMINT-8300912.
    STRINGi3702.AT3G14110.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ940U6.
    SMRiQ940U6. Positions 210-312.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei125 – 14521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati203 – 23634TPR 1Add
    BLAST
    Repeati243 – 27634TPR 2Add
    BLAST
    Repeati283 – 31634TPR 3Add
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili144 – 17532Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 3 TPR repeats.Curated

    Keywords - Domaini

    Coiled coil, Repeat, TPR repeat, Transit peptide, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG288145.
    HOGENOMiHOG000239198.
    InParanoidiQ940U6.
    PhylomeDBiQ940U6.

    Family and domain databases

    Gene3Di1.25.40.10. 1 hit.
    InterProiIPR013026. TPR-contain_dom.
    IPR011990. TPR-like_helical.
    [Graphical view]
    PROSITEiPS50293. TPR_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q940U6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAALIRCCSS FSHTSGGQPP PRDKSRAPEI GKFATSIGYS VVRKPGDHPP    50
    FSKIIHSSSQ PKERQGKGIL QTPFASVGSL DKFSAFEGIG RLKLPVMAVL 100
    LTNSLQMATP LEALAAEICE PESSMFSMPI LLLVALIGAT VGGLLARQRK 150
    GELQRLNEQL RQINAALRRQ AKIESYAPSL SYAPVGARIP DSEIIVEPKK 200
    QELISKLKTG KTFLRNQEPE KAYTEFKIAL ELAQSLKDPT EEKKAARGLG 250
    ASLQRQGKYR EAIQYHSMVL AISKRESEDS GITEAYGAIA DCYTELGDLE 300
    KAGKFYDTYI ARLETD 316
    Length:316
    Mass (Da):34,587
    Last modified:December 1, 2001 - v1
    Checksum:i8A647B74684B6514
    GO

    Sequence cautioni

    The sequence BAB02975.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti97 – 971M → L in BAH20437. (PubMed:19423640)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP000600 Genomic DNA. Translation: BAB02975.1. Sequence problems.
    CP002686 Genomic DNA. Translation: AEE75470.1.
    AF446890 mRNA. Translation: AAL38623.1.
    AY052677 mRNA. Translation: AAK96581.1.
    AY087529 mRNA. Translation: AAM65071.1.
    AK317782 mRNA. Translation: BAH20437.1.
    RefSeqiNP_566478.1. NM_112267.3. [Q940U6-1]
    UniGeneiAt.22594.
    At.70203.

    Genome annotation databases

    EnsemblPlantsiAT3G14110.1; AT3G14110.1; AT3G14110. [Q940U6-1]
    GeneIDi820628.
    KEGGiath:AT3G14110.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP000600 Genomic DNA. Translation: BAB02975.1 . Sequence problems.
    CP002686 Genomic DNA. Translation: AEE75470.1 .
    AF446890 mRNA. Translation: AAL38623.1 .
    AY052677 mRNA. Translation: AAK96581.1 .
    AY087529 mRNA. Translation: AAM65071.1 .
    AK317782 mRNA. Translation: BAH20437.1 .
    RefSeqi NP_566478.1. NM_112267.3. [Q940U6-1 ]
    UniGenei At.22594.
    At.70203.

    3D structure databases

    ProteinModelPortali Q940U6.
    SMRi Q940U6. Positions 210-312.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 5962. 1 interaction.
    IntActi Q940U6. 5 interactions.
    MINTi MINT-8300912.
    STRINGi 3702.AT3G14110.1-P.

    Proteomic databases

    PaxDbi Q940U6.
    PRIDEi Q940U6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G14110.1 ; AT3G14110.1 ; AT3G14110 . [Q940U6-1 ]
    GeneIDi 820628.
    KEGGi ath:AT3G14110.

    Organism-specific databases

    TAIRi AT3G14110.

    Phylogenomic databases

    eggNOGi NOG288145.
    HOGENOMi HOG000239198.
    InParanoidi Q940U6.
    PhylomeDBi Q940U6.

    Gene expression databases

    ArrayExpressi Q940U6.
    Genevestigatori Q940U6.

    Family and domain databases

    Gene3Di 1.25.40.10. 1 hit.
    InterProi IPR013026. TPR-contain_dom.
    IPR011990. TPR-like_helical.
    [Graphical view ]
    PROSITEi PS50293. TPR_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
      Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
      DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    4. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    5. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
      Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
      DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 97-316 (ISOFORM 1).
      Strain: cv. Columbia.
      Tissue: Flower and Silique.
    6. "FLU: a negative regulator of chlorophyll biosynthesis in Arabidopsis thaliana."
      Meskauskiene R., Nater M., Goslings D., Kessler F., op den Camp R.G.L., Apel K.
      Proc. Natl. Acad. Sci. U.S.A. 98:12826-12831(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF ALA-146 AND ALA-262, SUBCELLULAR LOCATION.
      Strain: cv. Landsberg erecta.
    7. "Proteomic study of the Arabidopsis thaliana chloroplastic envelope membrane utilizing alternatives to traditional two-dimensional electrophoresis."
      Froehlich J.E., Wilkerson C.G., Ray W.K., McAndrew R.S., Osteryoung K.W., Gage D.A., Phinney B.S.
      J. Proteome Res. 2:413-425(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. "Rapid induction of distinct stress responses after the release of singlet oxygen in Arabidopsis."
      op den Camp R.G.L., Przybyla D., Ochsenbein C., Laloi C., Kim C., Danon A., Wagner D., Hideg E., Goebel C., Feussner I., Nater M., Apel K.
      Plant Cell 15:2320-2332(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    9. "The Arabidopsis thaliana chloroplast proteome reveals pathway abundance and novel protein functions."
      Kleffmann T., Russenberger D., von Zychlinski A., Christopher W., Sjoelander K., Gruissem W., Baginsky S.
      Curr. Biol. 14:354-362(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    10. "New functions of the thylakoid membrane proteome of Arabidopsis thaliana revealed by a simple, fast, and versatile fractionation strategy."
      Peltier J.-B., Ytterberg A.J., Sun Q., van Wijk K.J.
      J. Biol. Chem. 279:49367-49383(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    11. "Concurrent interactions of heme and FLU with Glu tRNA reductase (HEMA1), the target of metabolic feedback inhibition of tetrapyrrole biosynthesis, in dark- and light-grown Arabidopsis plants."
      Goslings D., Meskauskiene R., Kim C., Lee K.P., Nater M., Apel K.
      Plant J. 40:957-967(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF ALA-262, INTERACTION WITH HEMA1 AND HEMA2.
      Strain: cv. Landsberg erecta.
    12. "Enzymatic, but not non-enzymatic, 1O2-mediated peroxidation of polyunsaturated fatty acids forms part of the EXECUTER1-dependent stress response program in the flu mutant of Arabidopsis thaliana."
      Przybyla D., Goebel C., Imboden A., Hamberg M., Feussner I., Apel K.
      Plant J. 54:236-248(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
      Strain: cv. Columbia.
    13. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
      Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
      PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    14. "AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
      Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
      Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    15. "FLU, a negative feedback regulator of tetrapyrrole biosynthesis, is physically linked to the final steps of the Mg(++)-branch of this pathway."
      Kauss D., Bischof S., Steiner S., Apel K., Meskauskiene R.
      FEBS Lett. 586:211-216(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN THE FLU-CONTAINING CHLOROPLAST MEMBRANE COMPLEX.

    Entry informationi

    Entry nameiFLU_ARATH
    AccessioniPrimary (citable) accession number: Q940U6
    Secondary accession number(s): B9DI70, Q9LJH7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3