Q940Q8 (IX10L_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable beta-1,4-xylosyltransferase IRX10L
EC=2.4.2.-

Alternative name(s):
Glucuronoxylan glucuronosyltransferase 1
Short name=AtGUT1
Glucuronoxylan glucuronosyltransferase 2
Short name=AtGUT2
Protein IRREGULAR XYLEM 10-like
Xylan xylosyltransferase IRX10L

Gene names

Name:	IRX10L
Synonyms:	GUT1, GUT2
Ordered Locus Names:	At5g61840
ORF Names:	MAC9.17

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the synthesis of the hemicellulose glucuronoxylan, a major component of secondary cell walls. Probably involved in the elongation of glucuronoxylan xylosyl backbone. Ref.6 Ref.7
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Tissue specificity	Expressed in xylem cells in stems and roots. Ref.7
Disruption phenotype	No visible phenotype. Ref.6
Sequence similarities	Belongs to the glycosyltransferase 47 family.
Sequence caution	The sequence BAB10084.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW glucuronoxylan biosynthetic process Inferred from mutant phenotype Ref.6. Source: TAIR secondary cell wall biogenesis Inferred from mutant phenotype Ref.6. Source: TAIR
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glucuronoxylan glucuronosyltransferase activity Inferred from mutant phenotype Ref.6. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	Probable beta-1,4-xylosyltransferase IRX10L		PRO_0000407577
Regions
	Topological domain	1	1	Cytoplasmic Potential
	Transmembrane	2 – 22	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	23 – 415	393	Lumenal Potential
Amino acid modifications
	Glycosylation	142	1	N-linked (GlcNAc...) Potential
	Glycosylation	403	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	158	1	G → R in AAM64940. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q940Q8 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 0A7C9350CE253A5D	FASTA	415	47,195
	10 20 30 40 50 60 MKLSSCVLIF LLCNTFSSIS AFRLSRSQPT ERISGSAGDV LEDDPVGRLK VFVYELPSKY 70 80 90 100 110 120 NKKILQKDPR CLNHMFAAEI YMQRFLLSSP VRTLNPEEAD WFYVPVYTTC DLTPNGLPLP 130 140 150 160 170 180 FKSPRMMRSA IQLIASNWPY WNRTEGADHF FVVPHDFGAC FHYQEEKAIG RGILPLLQRA 190 200 210 220 230 240 TLVQTFGQRN HVCLKEGSIT VPPYAPPQKM QSHLIPEKTP RSIFVYFRGL FYDVGNDPEG 250 260 270 280 290 300 GYYARGARAA VWENFKDNPL FDISTEHPTT YYEDMQRAIF CLCPLGWAPW SPRLVEAVIF 310 320 330 340 350 360 GCIPVIIADD IVLPFADAIP WEDIGVFVDE KDVPYLDTIL TSIPPEVILR KQRLLANPSM 370 380 390 400 410 KQAMLFPQPA QPGDAFHQVL NGLARKLPHE RSVYLRPGEK LLNWTAGPVA DLKPW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones." Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:41-54(1998) [PubMed: 9628582] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"A pectin glucuronyltransferase gene is essential for intercellular attachment in the plant meristem." Iwai H., Masaoka N., Ishii T., Satoh S. Proc. Natl. Acad. Sci. U.S.A. 99:16319-16324(2002) [PubMed: 12451175] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 75-415.
[6]	"Characterization of IRX10 and IRX10-like reveals an essential role in glucuronoxylan biosynthesis in Arabidopsis." Brown D.M., Zhang Z., Stephens E., Dupree P., Turner S.R. Plant J. 57:732-746(2009) [PubMed: 18980662] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[7]	"Analysis of the Arabidopsis IRX9/IRX9-L and IRX14/IRX14-L pairs of glycosyltransferase genes reveals critical contributions to biosynthesis of the hemicellulose glucuronoxylan." Wu A.M., Hoernblad E., Voxeur A., Gerber L., Rihouey C., Lerouge P., Marchant A. Plant Physiol. 153:542-554(2010) [PubMed: 20424005] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB010069 Genomic DNA. Translation: BAB10084.1. Sequence problems. CP002688 Genomic DNA. Translation: AED97524.1. AY054180 mRNA. Translation: AAL06841.1. AY066039 mRNA. Translation: AAL47406.1. AY087390 mRNA. Translation: AAM64940.1. AB080693 mRNA. Translation: BAC20929.1.
IPI	IPI00546195.
RefSeq	NP_568941.1. NM_125578.2.
UniGene	At.8499.
3D structure databases
ProteinModelPortal	Q940Q8.
ModBase	Search...
Protein family/group databases
CAZy	GT47. Glycosyltransferase Family 47.
Proteomic databases
PRIDE	Q940Q8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G61840.1; AT5G61840.1; AT5G61840.
GeneID	836306.
GenomeReviews	Gene locus AT5G61840 in contig BA000015_GR.
KEGG	ath:AT5G61840.
Organism-specific databases
TAIR	At5g61840.
Phylogenomic databases
InParanoid	Q940Q8.
OMA	ILQKDPR.
PhylomeDB	Q940Q8.
ProtClustDB	CLSN2682887.
Gene expression databases
Genevestigator	Q940Q8.
Family and domain databases
InterPro	IPR004263. Exostosin. [Graphical view]
Pfam	PF03016. Exostosin. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IX10L_ARATH

Accession

Primary (citable) accession number: Q940Q8
Secondary accession number(s): Q8LB70, Q9FLS5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 3, 2011
Last sequence update:	December 1, 2001
Last modified:	December 14, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program