Reviewed, UniProtKB/Swiss-Prot Q940Q1 (PEL1_ARATH)

Names and origin

Protein names

Recommended name:
    Probable pectate lyase 1
    EC=4.2.2.2
Alternative name(s):
    Pectate lyase A1

Gene names

Ordered Locus Names:	At1g04680
ORF Names:	T1G11.7, T1G11_6

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion. Required for its activity By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Tissue specificity	Expressed in flowers, but not in leaves. Ref.4
Sequence similarities	Belongs to the polysaccharide lyase 1 family.
Sequence caution	The sequence AAL06861.1 differs from that shown. Reason: Frameshift at position 351. The sequence AAL47400.1 differs from that shown. Reason: Frameshift at position 351.

Ontologies

Keywords
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	membrane Inferred from direct assay. Source: TAIR
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 431	411	Probable pectate lyase 1		PRO_0000024865
Sites
	Active site	307	1	Potential
	Metal binding	227	1	Calcium By similarity
	Metal binding	251	1	Calcium By similarity
	Metal binding	255	1	Calcium By similarity
Amino acid modifications
	Glycosylation	23	1	N-linked (GlcNAc...) Potential
	Glycosylation	28	1	N-linked (GlcNAc...) Potential
	Glycosylation	65	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	151	1	V → I in AAB69760. Ref.4
	Sequence conflict	208	1	Missing in AAB69760. Ref.4
	Sequence conflict	255 – 257	3	DAV → ERS in AAB69760. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q940Q1-1 [UniParc]. Last modified June 16, 2003. Version 2. Checksum: 241D3FD68D55C832	FASTA	431	47,771
	10 20 30 40 50 60 MAVLPTWLLA MMCLLFFVGA MENTTHDNIS SLPRSDETEW NQHAVTNPDE VADEVLALTE 70 80 90 100 110 120 MSVRNHTERR KLGYFTCGTG NPIDDCWRCD PNWHKNRKRL ADCGIGFGRN AIGGRDGRFY 130 140 150 160 170 180 VVTDPRDDNP VNPRPGTLRH AVIQDRPLWI VFKRDMVIQL KQELIVNSFK TIDGRGANVH 190 200 210 220 230 240 IANGGCITIQ FVTNVIVHGL HIHDCKPTGN AMVRSSETHF GWRTMADGDA ISIFGSSHVW 250 260 270 280 290 300 IDHNSLSHCA DGLVDAVMGS TAITISNNHL THHNEVMLLG HSDSYMRDKA MQVTIAYNHF 310 320 330 340 350 360 GVGLIQRMPR CRHGYFHVVN NDYTHWEMYA IGGSANPTIN SQGNRYAAPK NPFAKEVTKR 370 380 390 400 410 420 VDTPASHWKG WNWRSEGDLL QNGAYFTSSG AAASGSYARA SSLSAKSSSL VGHITSDAGA 430 LPCRRGRQCS S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Identification of the tobacco and Arabidopsis homologues of the pollen-expressed LAT59 gene of tomato." Kulikauskas R., McCormick S. Plant Mol. Biol. 34:809-814(1997) [PubMed: 9278171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 94-275, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC002376 Genomic DNA. Translation: AAB80622.1. AY065034 mRNA. Translation: AAL57671.1. AY054200 mRNA. Translation: AAL06861.1. Frameshift. AY066033 mRNA. Translation: AAL47400.1. Frameshift. AY087724 mRNA. Translation: AAM65261.1. U83620 Genomic DNA. Translation: AAB69760.1.
IPI	IPI00526911.
PIR	F86179.
RefSeq	NP_563715.1.
UniGene	At.10458
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Proteomic databases
PRIDE	Q940Q1.
Genome annotation databases
GeneID	839452.
GenomeReviews	Gene locus AT1G04680 in contig CT485782_GR.
KEGG	ath:AT1G04680.
NMPDR	fig|3702.1.peg.618.
Organism-specific databases
TAIR	At1g04680.
Phylogenomic databases
OMA	RCDPNWH.
Enzyme and pathway databases
BRENDA	4.2.2.2. 302.
Gene expression databases
ArrayExpress	Q940Q1.
Genevestigator	Q940Q1.
GermOnline	AT1G04680. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR018082. AmbAllergen. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
PRINTS	PR00807. AMBALLERGEN.
SMART	SM00656. Amb_all. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PEL1_ARATH

Accession

Primary (citable) accession number: Q940Q1
Secondary accession number(s): O23017, O23666

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	June 16, 2003
Last modified:	November 3, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)