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Protein

Protein kinase and PP2C-like domain-containing protein

Gene

At2g40860/At2g40870

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei57 – 571ATPPROSITE-ProRule annotation
Active sitei149 – 1491Proton acceptorPROSITE-ProRule annotation
Metal bindingi428 – 4281Manganese 1By similarity
Metal bindingi428 – 4281Manganese 2By similarity
Metal bindingi429 – 4291Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi599 – 5991Manganese 2By similarity
Metal bindingi639 – 6391Manganese 2By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi36 – 449ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoprotein phosphatase activity Source: UniProtKB-KW
  4. protein serine/threonine kinase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Protein phosphatase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G40860-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase and PP2C-like domain-containing protein
Including the following 2 domains:
Probable serine/threonine protein kinase At2g40860 (EC:2.7.11.1)
Probable protein phosphatase 2C 31 (EC:3.1.3.16)
Short name:
AtPP2C31
Gene namesi
Ordered Locus Names:At2g40860/At2g40870
ORF Names:T20B5.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G40860.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 658658Protein kinase and PP2C-like domain-containing proteinPRO_0000367959Add
BLAST

Proteomic databases

PaxDbiQ940A2.
PRIDEiQ940A2.

Expressioni

Gene expression databases

GenevestigatoriQ940A2.

Structurei

3D structure databases

ProteinModelPortaliQ940A2.
SMRiQ940A2. Positions 29-304, 388-650.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 314285Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini392 – 648257PPM-type phosphatasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.Curated
In the C-terminal section; belongs to the PP2C family.Curated
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000012809.
InParanoidiQ940A2.
OMAiNWRSSGK.
PhylomeDBiQ940A2.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR001932. PP2C-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
SSF81606. SSF81606. 1 hit.
PROSITEiPS51746. PPM_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q940A2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVMEIVKPNT CIRGCCTSES IPLHLPSSSF TLLSPIAKGS ESVVYEAILD
60 70 80 90 100
GRRVAAKKPI LSTSDDLDKF HRNLQLSCNL NHPGVAKLLA AHAKPPNYMF
110 120 130 140 150
FFDFYESGTL AEKLHVEEWS PSIDQVLLIT LHLAKALQYL HNNGIVHRDV
160 170 180 190 200
KPANVLLDEK FFPYLADFGL AEYKKNLREV NLQNWRSSGK PTGGFHKKNM
210 220 230 240 250
VGTLIYMAPE ILRKDMYTEK ADIYSFGILI NELLTGVVPY TDRRAEAQAH
260 270 280 290 300
TVLEMNYTEQ QLTVAIVSSG LRPALAEIGL HLPKSLLSLI QNCWESDPSK
310 320 330 340 350
RPSSDNVVLE LESIWEQVRG KQQGHLLEKT SNSQSDTDGA DIIKNSGDYR
360 370 380 390 400
DTVNWFSQGE CLSKKSSVST VFDVKLWSSS TDEPSRYVPV ISCGSFATCG
410 420 430 440 450
RRESMEDTHF IIPHMCNEES IHLFAIFDGH RGAAAAEFSA QVLPGLVQSL
460 470 480 490 500
CSTSAGEALS QAFVRTDLAF RQELDSHRQS KRVSQKDWHP GCTAIASLLV
510 520 530 540 550
ENKLFVANVG DSRAILCRAG HPFALSKAHL ATCIDERNRV IGEGGRIEWL
560 570 580 590 600
VDTWRVAPAG LQVTRSIGDD DLKPAVTAEP EISETILSAD DEFLVMASDG
610 620 630 640 650
LWDVMNDEEV IGIIRDTVKE PSMCSKRLAT EAAARGSGDN ITVIVVFLRP

VSTAERIY
Length:658
Mass (Da):72,853
Last modified:December 1, 2001 - v1
Checksum:i66041EE6EAEFEE14
GO

Sequence cautioni

The sequence AAB86446.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At2g40860 and At2g40870.Curated
The sequence AAB86447.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At2g40860 and At2g40870.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002409 Genomic DNA. Translation: AAB86446.1. Sequence problems.
AC002409 Genomic DNA. Translation: AAB86447.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC09891.1.
AY056151 mRNA. Translation: AAL07230.1.
AY133729 mRNA. Translation: AAM91663.1.
PIRiT00750.
T00751.
RefSeqiNP_850336.1. NM_180005.3.
UniGeneiAt.42906.

Genome annotation databases

EnsemblPlantsiAT2G40860.1; AT2G40860.1; AT2G40860.
GeneIDi818684.
KEGGiath:AT2G40860.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002409 Genomic DNA. Translation: AAB86446.1. Sequence problems.
AC002409 Genomic DNA. Translation: AAB86447.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC09891.1.
AY056151 mRNA. Translation: AAL07230.1.
AY133729 mRNA. Translation: AAM91663.1.
PIRiT00750.
T00751.
RefSeqiNP_850336.1. NM_180005.3.
UniGeneiAt.42906.

3D structure databases

ProteinModelPortaliQ940A2.
SMRiQ940A2. Positions 29-304, 388-650.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiQ940A2.
PRIDEiQ940A2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G40860.1; AT2G40860.1; AT2G40860.
GeneIDi818684.
KEGGiath:AT2G40860.

Organism-specific databases

TAIRiAT2G40860.

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000012809.
InParanoidiQ940A2.
OMAiNWRSSGK.
PhylomeDBiQ940A2.

Enzyme and pathway databases

BioCyciARA:AT2G40860-MONOMER.

Miscellaneous databases

PROiQ940A2.

Gene expression databases

GenevestigatoriQ940A2.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR001932. PP2C-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
SSF81606. SSF81606. 1 hit.
PROSITEiPS51746. PPM_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
    Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
    BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiP2C31_ARATH
AccessioniPrimary (citable) accession number: Q940A2
Secondary accession number(s): O22200, O22201
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: December 1, 2001
Last modified: April 29, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.