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Q940A2 (P2C31_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein kinase and PP2C-like domain-containing protein

Including the following 2 domains:

  1. Probable serine/threonine protein kinase At2g40860
    EC=2.7.11.1
  2. Probable protein phosphatase 2C 31
    Short name=AtPP2C31
    EC=3.1.3.16
Gene names
Ordered Locus Names:At2g40860/At2g40870
ORF Names:T20B5.6
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length658 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Sequence similarities

In the N-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

In the C-terminal section; belongs to the PP2C family.

Contains 1 PP2C-like domain.

Contains 1 protein kinase domain.

Sequence caution

The sequence AAB86446.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At2g40860 and At2g40870.

The sequence AAB86447.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At2g40860 and At2g40870.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 658658Protein kinase and PP2C-like domain-containing protein
PRO_0000367959

Regions

Domain30 – 314285Protein kinase
Domain391 – 641251PP2C-like
Nucleotide binding36 – 449ATP By similarity

Sites

Active site1491Proton acceptor By similarity
Metal binding4281Manganese 1 By similarity
Metal binding4281Manganese 2 By similarity
Metal binding4291Manganese 1; via carbonyl oxygen By similarity
Metal binding5991Manganese 2 By similarity
Metal binding6391Manganese 2 By similarity
Binding site571ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q940A2 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 66041EE6EAEFEE14

FASTA65872,853
        10         20         30         40         50         60 
MVMEIVKPNT CIRGCCTSES IPLHLPSSSF TLLSPIAKGS ESVVYEAILD GRRVAAKKPI 

        70         80         90        100        110        120 
LSTSDDLDKF HRNLQLSCNL NHPGVAKLLA AHAKPPNYMF FFDFYESGTL AEKLHVEEWS 

       130        140        150        160        170        180 
PSIDQVLLIT LHLAKALQYL HNNGIVHRDV KPANVLLDEK FFPYLADFGL AEYKKNLREV 

       190        200        210        220        230        240 
NLQNWRSSGK PTGGFHKKNM VGTLIYMAPE ILRKDMYTEK ADIYSFGILI NELLTGVVPY 

       250        260        270        280        290        300 
TDRRAEAQAH TVLEMNYTEQ QLTVAIVSSG LRPALAEIGL HLPKSLLSLI QNCWESDPSK 

       310        320        330        340        350        360 
RPSSDNVVLE LESIWEQVRG KQQGHLLEKT SNSQSDTDGA DIIKNSGDYR DTVNWFSQGE 

       370        380        390        400        410        420 
CLSKKSSVST VFDVKLWSSS TDEPSRYVPV ISCGSFATCG RRESMEDTHF IIPHMCNEES 

       430        440        450        460        470        480 
IHLFAIFDGH RGAAAAEFSA QVLPGLVQSL CSTSAGEALS QAFVRTDLAF RQELDSHRQS 

       490        500        510        520        530        540 
KRVSQKDWHP GCTAIASLLV ENKLFVANVG DSRAILCRAG HPFALSKAHL ATCIDERNRV 

       550        560        570        580        590        600 
IGEGGRIEWL VDTWRVAPAG LQVTRSIGDD DLKPAVTAEP EISETILSAD DEFLVMASDG 

       610        620        630        640        650 
LWDVMNDEEV IGIIRDTVKE PSMCSKRLAT EAAARGSGDN ITVIVVFLRP VSTAERIY 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC002409 Genomic DNA. Translation: AAB86446.1. Sequence problems.
AC002409 Genomic DNA. Translation: AAB86447.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC09891.1.
AY056151 mRNA. Translation: AAL07230.1.
AY133729 mRNA. Translation: AAM91663.1.
PIRT00750.
T00751.
RefSeqNP_850336.1. NM_180005.3.
UniGeneAt.42906.

3D structure databases

ProteinModelPortalQ940A2.
SMRQ940A2. Positions 51-303, 388-650.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ940A2.
PRIDEQ940A2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G40860.1; AT2G40860.1; AT2G40860.
GeneID818684.
KEGGath:AT2G40860.

Organism-specific databases

TAIRAT2G40860.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000012809.
InParanoidQ940A2.
OMAVPYTDLR.
PhylomeDBQ940A2.
ProtClustDBCLSN2680164.

Enzyme and pathway databases

BioCycARA:AT2G40860-MONOMER.

Gene expression databases

GenevestigatorQ940A2.

Family and domain databases

Gene3D3.60.40.10. 1 hit.
InterProIPR011009. Kinase-like_dom.
IPR001932. PP2C-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
PF00481. PP2C. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
SSF81606. SSF81606. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameP2C31_ARATH
AccessionPrimary (citable) accession number: Q940A2
Secondary accession number(s): O22200, O22201
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names