Q940A2 (P2C31_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein kinase and PP2C-like domain-containing protein

Including the following 2 domains:

1. Probable serine/threonine protein kinase At2g40860
   EC=2.7.11.1
2. Probable protein phosphatase 2C 31
   Short name=AtPP2C31
   EC=3.1.3.16

Gene names

Ordered Locus Names:	At2g40860/At2g40870
ORF Names:	T20B5.6

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	658 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein. A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Sequence similarities	In the N-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family. In the C-terminal section; belongs to the PP2C family. Contains 1 PP2C-like domain. Contains 1 protein kinase domain.
Sequence caution	The sequence AAB86446.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At2g40860 and At2g40870. The sequence AAB86447.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At2g40860 and At2g40870.

Ontologies

Keywords
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 658	658	Protein kinase and PP2C-like domain-containing protein		PRO_0000367959
Regions
	Domain	30 – 314	285	Protein kinase
	Domain	391 – 641	251	PP2C-like
	Nucleotide binding	36 – 44	9	ATP By similarity
Sites
	Active site	149	1	Proton acceptor By similarity
	Metal binding	428	1	Manganese 1 By similarity
	Metal binding	428	1	Manganese 2 By similarity
	Metal binding	429	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	599	1	Manganese 2 By similarity
	Metal binding	639	1	Manganese 2 By similarity
	Binding site	57	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q940A2 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 66041EE6EAEFEE14	FASTA	658	72,853
	10 20 30 40 50 60 MVMEIVKPNT CIRGCCTSES IPLHLPSSSF TLLSPIAKGS ESVVYEAILD GRRVAAKKPI 70 80 90 100 110 120 LSTSDDLDKF HRNLQLSCNL NHPGVAKLLA AHAKPPNYMF FFDFYESGTL AEKLHVEEWS 130 140 150 160 170 180 PSIDQVLLIT LHLAKALQYL HNNGIVHRDV KPANVLLDEK FFPYLADFGL AEYKKNLREV 190 200 210 220 230 240 NLQNWRSSGK PTGGFHKKNM VGTLIYMAPE ILRKDMYTEK ADIYSFGILI NELLTGVVPY 250 260 270 280 290 300 TDRRAEAQAH TVLEMNYTEQ QLTVAIVSSG LRPALAEIGL HLPKSLLSLI QNCWESDPSK 310 320 330 340 350 360 RPSSDNVVLE LESIWEQVRG KQQGHLLEKT SNSQSDTDGA DIIKNSGDYR DTVNWFSQGE 370 380 390 400 410 420 CLSKKSSVST VFDVKLWSSS TDEPSRYVPV ISCGSFATCG RRESMEDTHF IIPHMCNEES 430 440 450 460 470 480 IHLFAIFDGH RGAAAAEFSA QVLPGLVQSL CSTSAGEALS QAFVRTDLAF RQELDSHRQS 490 500 510 520 530 540 KRVSQKDWHP GCTAIASLLV ENKLFVANVG DSRAILCRAG HPFALSKAHL ATCIDERNRV 550 560 570 580 590 600 IGEGGRIEWL VDTWRVAPAG LQVTRSIGDD DLKPAVTAEP EISETILSAD DEFLVMASDG 610 620 630 640 650 LWDVMNDEEV IGIIRDTVKE PSMCSKRLAT EAAARGSGDN ITVIVVFLRP VSTAERIY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC002409 Genomic DNA. Translation: AAB86446.1. Sequence problems. AC002409 Genomic DNA. Translation: AAB86447.1. Sequence problems. CP002685 Genomic DNA. Translation: AEC09891.1. AY056151 mRNA. Translation: AAL07230.1. AY133729 mRNA. Translation: AAM91663.1.
IPI	IPI00539299.
PIR	T00750. T00751.
RefSeq	NP_850336.1. NM_180005.3.
UniGene	At.42906.
3D structure databases
ProteinModelPortal	Q940A2.
SMR	Q940A2. Positions 8-314, 394-650.
ModBase	Search...
Proteomic databases
PaxDb	Q940A2.
PRIDE	Q940A2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G40860.1; AT2G40860.1; AT2G40860.
GeneID	818684.
KEGG	ath:AT2G40860.
Organism-specific databases
TAIR	At2g40860.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000012809.
InParanoid	Q940A2.
OMA	NWRSSGK.
PhylomeDB	Q940A2.
ProtClustDB	CLSN2680164.
Gene expression databases
Genevestigator	Q940A2.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR011009. Kinase-like_dom. IPR001932. PP2C-like. IPR000719. Prot_kinase_cat_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. False negative. PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C31_ARATH

Accession

Primary (citable) accession number: Q940A2
Secondary accession number(s): O22200, O22201

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program