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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A

Gene

STT3A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3A seems to be involved in complex substrate specificity (By similarity). Controls adaptive responses to salt/osmotic stress. Acts as a key glycosylation determinant for EFR function during plant innate immunity.By similarity3 Publications

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • post-translational protein modification Source: GO_Central
  • protein N-linked glycosylation via asparagine Source: GO_Central
  • regulation of response to osmotic stress Source: UniProtKB
  • response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT5G19690-MONOMER.
BRENDAi2.4.99.18. 399.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT66. Glycosyltransferase Family 66.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A (EC:2.4.99.18)
Short name:
Oligosaccharyl transferase subunit STT3A
Short name:
STT3-A
Alternative name(s):
Integral membrane protein 1
Protein STAUROSPORIN AND TEMPERATURE SENSITIVE 3-LIKE A
Gene namesi
Name:STT3A
Ordered Locus Names:At5g19690
ORF Names:T29J13_110
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G19690.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1717CytoplasmicSequence analysisAdd
BLAST
Transmembranei18 – 3821HelicalSequence analysisAdd
BLAST
Topological domaini39 – 11981LumenalSequence analysisAdd
BLAST
Transmembranei120 – 14021HelicalSequence analysisAdd
BLAST
Topological domaini141 – 17434CytoplasmicSequence analysisAdd
BLAST
Transmembranei175 – 19521HelicalSequence analysisAdd
BLAST
Topological domaini196 – 21318LumenalSequence analysisAdd
BLAST
Transmembranei214 – 23421HelicalSequence analysisAdd
BLAST
Topological domaini235 – 2428CytoplasmicSequence analysis
Transmembranei243 – 26321HelicalSequence analysisAdd
BLAST
Topological domaini264 – 27411LumenalSequence analysisAdd
BLAST
Transmembranei275 – 29521HelicalSequence analysisAdd
BLAST
Topological domaini296 – 30510CytoplasmicSequence analysis
Transmembranei306 – 32621HelicalSequence analysisAdd
BLAST
Topological domaini327 – 36640LumenalSequence analysisAdd
BLAST
Transmembranei367 – 38721HelicalSequence analysisAdd
BLAST
Topological domaini388 – 3947CytoplasmicSequence analysis
Transmembranei395 – 41218HelicalSequence analysisAdd
BLAST
Topological domaini413 – 4131LumenalSequence analysis
Transmembranei414 – 43421HelicalSequence analysisAdd
BLAST
Topological domaini435 – 51884CytoplasmicSequence analysisAdd
BLAST
Transmembranei519 – 53921HelicalSequence analysisAdd
BLAST
Topological domaini540 – 779240LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: TAIR
  • oligosaccharyltransferase complex Source: GO_Central
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Salt/osmotic sensitivity associated with root tip growth arrest and swelling and the induction of lateral roots.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 779779Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3APRO_0000420536Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi597 – 5971N-linked (GlcNAc...)Sequence analysis
Glycosylationi604 – 6041N-linked (GlcNAc...)Sequence analysis
Glycosylationi608 – 6081N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ93ZY3.
PRIDEiQ93ZY3.

PTM databases

SwissPalmiQ93ZY3.

Expressioni

Tissue specificityi

Expressed in the root and in the shoot.1 Publication

Gene expression databases

ExpressionAtlasiQ93ZY3. baseline and differential.
GenevisibleiQ93ZY3. AT.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex.By similarity

Protein-protein interaction databases

STRINGi3702.AT5G19690.1.

Structurei

3D structure databases

ProteinModelPortaliQ93ZY3.
SMRiQ93ZY3. Positions 535-777.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the STT3 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2292. Eukaryota.
COG1287. LUCA.
HOGENOMiHOG000157471.
InParanoidiQ93ZY3.
KOiK07151.
OMAiYINDDIR.
PhylomeDBiQ93ZY3.

Family and domain databases

InterProiIPR003674. Oligo_trans_STT3.
[Graphical view]
PfamiPF02516. STT3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q93ZY3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALESPLPG TPTAMRNAFG NVLSVLILVL IGVLAFSIRL FSVIKYESVI
60 70 80 90 100
HEFDPYFNYR VTQFLSKNGI YEFWNWFDDR TWYPLGRVIG GTVYPGLTLT
110 120 130 140 150
AGTIWWGLNS LNIPLSVETV CVFTAPVFSA FASWATYLLT KEVKGSGAGL
160 170 180 190 200
AAAALLAMVP SYISRSVAGS YDNEAVAIFA LIFTFYLYIK TLNTGSLFYA
210 220 230 240 250
TLNALAYFYM VCSWGGYTFI INLIPMHVLL CIVTGRYSPR LYIAYAPLVV
260 270 280 290 300
LGTLLAALVP VVGFNAVLTS EHFASFLVFI IIHVVALVYY IKGILSPKMF
310 320 330 340 350
KVAVTLVVSI GMVVCFIVVA ILVALVASSP TGGWSGRSLS LLDPTYASKY
360 370 380 390 400
IPIIASVSEH QPPTWPSYFM DINVLAFLVP AGIIACFSPL SDASSFVVLY
410 420 430 440 450
IVMSVYFSGV MVRLMLVLAP AACIMSGIAL SQAFDVFTGS IKYQLGASSN
460 470 480 490 500
STDDAEDNTS TNNAPKDDVS AGKTDKGEEI VKERSSKKGK KKEREPADKP
510 520 530 540 550
SVKAKIKKKA LVLPLEASIV ALLLLIMLGA FYVIHCVWAA AEAYSAPSIV
560 570 580 590 600
LTSQSRDGLH VFDDFRESYA WLSHNTDVDD KVASWWDYGY QTTAMANRTV
610 620 630 640 650
IVDNNTWNNT HIATVGTAMS SPEKAAWEIF NSLDVKYVLV VFGGLIGYPS
660 670 680 690 700
DDINKFLWMV RIGGGVFPHI KEADYLRDGQ YRIDSEATPT MLNSLMYKLS
710 720 730 740 750
YYRFVETDGK GYDRVRRTEI GKKHFKLTHF EEVFTSHHWM VRLYKLKPPR
760 770
NRIRGKAKKL KSKTSSGLSS KSAKKNPWV
Length:779
Mass (Da):86,113
Last modified:December 1, 2001 - v1
Checksum:i74F702FF6772478B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti735 – 7351T → K in AAK91413 (PubMed:14593172).Curated
Sequence conflicti735 – 7351T → K in AAL31142 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF296838 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92738.1.
AY050397 mRNA. Translation: AAK91413.1.
AY056191 mRNA. Translation: AAL07040.1.
AY059649 mRNA. Translation: AAL31142.1.
RefSeqiNP_568380.1. NM_121974.4.
UniGeneiAt.23388.

Genome annotation databases

EnsemblPlantsiAT5G19690.1; AT5G19690.1; AT5G19690.
GeneIDi832089.
GrameneiAT5G19690.1; AT5G19690.1; AT5G19690.
KEGGiath:AT5G19690.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF296838 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92738.1.
AY050397 mRNA. Translation: AAK91413.1.
AY056191 mRNA. Translation: AAL07040.1.
AY059649 mRNA. Translation: AAL31142.1.
RefSeqiNP_568380.1. NM_121974.4.
UniGeneiAt.23388.

3D structure databases

ProteinModelPortaliQ93ZY3.
SMRiQ93ZY3. Positions 535-777.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G19690.1.

Protein family/group databases

CAZyiGT66. Glycosyltransferase Family 66.

PTM databases

SwissPalmiQ93ZY3.

Proteomic databases

PaxDbiQ93ZY3.
PRIDEiQ93ZY3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G19690.1; AT5G19690.1; AT5G19690.
GeneIDi832089.
GrameneiAT5G19690.1; AT5G19690.1; AT5G19690.
KEGGiath:AT5G19690.

Organism-specific databases

TAIRiAT5G19690.

Phylogenomic databases

eggNOGiKOG2292. Eukaryota.
COG1287. LUCA.
HOGENOMiHOG000157471.
InParanoidiQ93ZY3.
KOiK07151.
OMAiYINDDIR.
PhylomeDBiQ93ZY3.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciARA:AT5G19690-MONOMER.
BRENDAi2.4.99.18. 399.

Miscellaneous databases

PROiQ93ZY3.

Gene expression databases

ExpressionAtlasiQ93ZY3. baseline and differential.
GenevisibleiQ93ZY3. AT.

Family and domain databases

InterProiIPR003674. Oligo_trans_STT3.
[Graphical view]
PfamiPF02516. STT3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "The STT3a subunit isoform of the Arabidopsis oligosaccharyltransferase controls adaptive responses to salt/osmotic stress."
    Koiwa H., Li F., McCully M.G., Mendoza I., Koizumi N., Manabe Y., Nakagawa Y., Zhu J., Rus A., Pardo J.M., Bressan R.A., Hasegawa P.M.
    Plant Cell 15:2273-2284(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION, TISSUE SPECIFICITY.
    Strain: cv. C24 and cv. Columbia.
  5. Cited for: SUBCELLULAR LOCATION.
  6. Cited for: FUNCTION.
  7. "Receptor quality control in the endoplasmic reticulum for plant innate immunity."
    Saijo Y., Tintor N., Lu X., Rauf P., Pajerowska-Mukhtar K., Haeweker H., Dong X., Robatzek S., Schulze-Lefert P.
    EMBO J. 28:3439-3449(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Putative glycosyltransferases and other plant Golgi apparatus proteins are revealed by LOPIT proteomics."
    Nikolovski N., Rubtsov D., Segura M.P., Miles G.P., Stevens T.J., Dunkley T.P., Munro S., Lilley K.S., Dupree P.
    Plant Physiol. 160:1037-1051(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSTT3A_ARATH
AccessioniPrimary (citable) accession number: Q93ZY3
Secondary accession number(s): Q94A42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: December 1, 2001
Last modified: June 8, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.