Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glucose-6-phosphate 1-dehydrogenase 4, chloroplastic

Gene

At1g09420

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division.

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

Enzyme regulationi

Regulated by metabolites. Post-translationally inactivated by cysteine-mediated redox modification via the ferredoxin-thioredoxin system in the light and this avoids futile cycles with photosynthetic CO2 fixation (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei194 – 1941NADPBy similarity
Binding sitei297 – 2971NADP; via carbonyl oxygenBy similarity
Binding sitei297 – 2971SubstrateBy similarity
Binding sitei365 – 3651SubstrateBy similarity
Binding sitei382 – 3821SubstrateBy similarity
Active sitei387 – 3871Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi160 – 1678NADPBy similarity

GO - Molecular functioni

  1. glucose-6-phosphate dehydrogenase activity Source: UniProtKB-EC
  2. NADP binding Source: InterPro

GO - Biological processi

  1. glucose metabolic process Source: UniProtKB-KW
  2. pentose-phosphate shunt Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciARA:AT1G09420-MONOMER.
ARA:GQT-74-MONOMER.
UniPathwayiUPA00115; UER00408.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenase 4, chloroplastic (EC:1.1.1.49)
Short name:
G6PD4
Short name:
G6PDH4
Gene namesi
Ordered Locus Names:At1g09420
ORF Names:F14J9.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G09420.

Subcellular locationi

Plastidchloroplast By similarity

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4949ChloroplastSequence AnalysisAdd
BLAST
Chaini50 – 625576Glucose-6-phosphate 1-dehydrogenase 4, chloroplasticPRO_0000010438Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi212 ↔ 220Redox modulationBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ93ZW0.
PRIDEiQ93ZW0.

Expressioni

Gene expression databases

ExpressionAtlasiQ93ZW0. baseline and differential.
GenevestigatoriQ93ZW0.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi22706. 2 interactions.
STRINGi3702.AT1G09420.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ93ZW0.
SMRiQ93ZW0. Positions 158-622.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni327 – 3315Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0364.
HOGENOMiHOG000046192.
InParanoidiQ93ZW0.
KOiK00036.
PhylomeDBiQ93ZW0.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD.
InterProiIPR001282. G6P_DH.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PRINTSiPR00079. G6PDHDRGNASE.
TIGRFAMsiTIGR00871. zwf. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q93ZW0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLSSCLLPF SQSATAPSSS VCSCHLAASF SNFPVSSRDY SFSRSGSLVL
60 70 80 90 100
NGGGSNLCRR FCGLKLWILK SLNRRQGNNR KHQPVNELTT HSKHTFLSDD
110 120 130 140 150
ERGFAEETRA EDLRPEENIL GTDLNDGFHN VGDLPPVSKQ LSDDLSDVRR
160 170 180 190 200
RASLCIAVVG ATGELARGKI FPALFALYYS GYLPEDVAIF GVSRKNLTDE
210 220 230 240 250
DLRSIIASTL TCRVDHQENC GGKMDAFQSR TYYINGGYNN RDGMSRLAER
260 270 280 290 300
MKQIEGESEA NRIFYLSVPQ EALVDVACTI GDNAQAPRGW TRIIVEKPFG
310 320 330 340 350
FNSHSSHQLT KSLLSKFEEK QIYRIDHMLG RNLIENLTVL RFSNLVFEPL
360 370 380 390 400
WNRTYIRNIQ VIISESIAQT EKFSDGYGII RDIVHSHILQ TIALLAMEPP
410 420 430 440 450
ISLDGEDIRN EKVKVLRSIR KIDPRDVILG QYKSSSRDKN GVILNGVDPT
460 470 480 490 500
YCAAALYIDN ARWDGVPFLV RVGTGLIKHR VEIHVQFRHV PGNLYRENIG
510 520 530 540 550
INIDLGTNEL ILRDEPDEAI LVKINNKVPG LGLQLDASEL NLLYKDRYKT
560 570 580 590 600
EVPDSYEHLI HDVIDGDNHL FMRSDEVAAA WNILSPVLEE IDKHHTAPEL
610 620
YEFGGRGPVA AYYLWAKHGV PWADD
Length:625
Mass (Da):70,202
Last modified:December 1, 2001 - v1
Checksum:i83D9F8D96F0B66F4
GO

Sequence cautioni

The sequence AAC33202.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003970 Genomic DNA. Translation: AAC33202.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28440.1.
AY056232 mRNA. Translation: AAL07081.1.
AY117271 mRNA. Translation: AAM51346.1.
PIRiE86227.
RefSeqiNP_563844.1. NM_100813.2. [Q93ZW0-1]
UniGeneiAt.26355.

Genome annotation databases

EnsemblPlantsiAT1G09420.1; AT1G09420.1; AT1G09420. [Q93ZW0-1]
GeneIDi837465.
KEGGiath:AT1G09420.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003970 Genomic DNA. Translation: AAC33202.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28440.1.
AY056232 mRNA. Translation: AAL07081.1.
AY117271 mRNA. Translation: AAM51346.1.
PIRiE86227.
RefSeqiNP_563844.1. NM_100813.2. [Q93ZW0-1]
UniGeneiAt.26355.

3D structure databases

ProteinModelPortaliQ93ZW0.
SMRiQ93ZW0. Positions 158-622.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22706. 2 interactions.
STRINGi3702.AT1G09420.1-P.

Proteomic databases

PaxDbiQ93ZW0.
PRIDEiQ93ZW0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G09420.1; AT1G09420.1; AT1G09420. [Q93ZW0-1]
GeneIDi837465.
KEGGiath:AT1G09420.

Organism-specific databases

TAIRiAT1G09420.

Phylogenomic databases

eggNOGiCOG0364.
HOGENOMiHOG000046192.
InParanoidiQ93ZW0.
KOiK00036.
PhylomeDBiQ93ZW0.

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.
BioCyciARA:AT1G09420-MONOMER.
ARA:GQT-74-MONOMER.

Gene expression databases

ExpressionAtlasiQ93ZW0. baseline and differential.
GenevestigatoriQ93ZW0.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD.
InterProiIPR001282. G6P_DH.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PRINTSiPR00079. G6PDHDRGNASE.
TIGRFAMsiTIGR00871. zwf. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiG6PD4_ARATH
AccessioniPrimary (citable) accession number: Q93ZW0
Secondary accession number(s): O80525
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: December 1, 2001
Last modified: March 4, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.