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Q93ZW0 (G6PD4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucose-6-phosphate 1-dehydrogenase 4, chloroplastic

Short name=G6PD4
Short name=G6PDH4
EC=1.1.1.49
Gene names
Ordered Locus Names:At1g09420
ORF Names:F14J9.8
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length625 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division. HAMAP-Rule MF_00966

Catalytic activity

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH. HAMAP-Rule MF_00966

Enzyme regulation

Regulated by metabolites. Post-translationally inactivated by cysteine-mediated redox modification via the ferredoxin-thioredoxin system in the light and this avoids futile cycles with photosynthetic CO2 fixation By similarity. HAMAP-Rule MF_00966

Pathway

Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3. HAMAP-Rule MF_00966

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00966

Subcellular location

Plastidchloroplast By similarity HAMAP-Rule MF_00966.

Miscellaneous

There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana.

Sequence similarities

Belongs to the glucose-6-phosphate dehydrogenase family.

Sequence caution

The sequence AAC33202.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Glucose metabolism
   Cellular componentChloroplast
Plastid
   Coding sequence diversityAlternative splicing
   DomainTransit peptide
   LigandNADP
   Molecular functionOxidoreductase
   PTMDisulfide bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpentose-phosphate shunt

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionNADP binding

Inferred from electronic annotation. Source: InterPro

glucose-6-phosphate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q93ZW0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4949Chloroplast Potential
Chain50 – 625576Glucose-6-phosphate 1-dehydrogenase 4, chloroplastic HAMAP-Rule MF_00966
PRO_0000010438

Regions

Nucleotide binding160 – 1678NADP By similarity
Region327 – 3315Substrate binding By similarity

Sites

Active site3871Proton acceptor By similarity
Binding site1941NADP By similarity
Binding site2971NADP; via carbonyl oxygen By similarity
Binding site2971Substrate By similarity
Binding site3651Substrate By similarity
Binding site3821Substrate By similarity

Amino acid modifications

Disulfide bond212 ↔ 220Redox modulation By similarity

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 83D9F8D96F0B66F4

FASTA62570,202
        10         20         30         40         50         60 
MSLSSCLLPF SQSATAPSSS VCSCHLAASF SNFPVSSRDY SFSRSGSLVL NGGGSNLCRR 

        70         80         90        100        110        120 
FCGLKLWILK SLNRRQGNNR KHQPVNELTT HSKHTFLSDD ERGFAEETRA EDLRPEENIL 

       130        140        150        160        170        180 
GTDLNDGFHN VGDLPPVSKQ LSDDLSDVRR RASLCIAVVG ATGELARGKI FPALFALYYS 

       190        200        210        220        230        240 
GYLPEDVAIF GVSRKNLTDE DLRSIIASTL TCRVDHQENC GGKMDAFQSR TYYINGGYNN 

       250        260        270        280        290        300 
RDGMSRLAER MKQIEGESEA NRIFYLSVPQ EALVDVACTI GDNAQAPRGW TRIIVEKPFG 

       310        320        330        340        350        360 
FNSHSSHQLT KSLLSKFEEK QIYRIDHMLG RNLIENLTVL RFSNLVFEPL WNRTYIRNIQ 

       370        380        390        400        410        420 
VIISESIAQT EKFSDGYGII RDIVHSHILQ TIALLAMEPP ISLDGEDIRN EKVKVLRSIR 

       430        440        450        460        470        480 
KIDPRDVILG QYKSSSRDKN GVILNGVDPT YCAAALYIDN ARWDGVPFLV RVGTGLIKHR 

       490        500        510        520        530        540 
VEIHVQFRHV PGNLYRENIG INIDLGTNEL ILRDEPDEAI LVKINNKVPG LGLQLDASEL 

       550        560        570        580        590        600 
NLLYKDRYKT EVPDSYEHLI HDVIDGDNHL FMRSDEVAAA WNILSPVLEE IDKHHTAPEL 

       610        620 
YEFGGRGPVA AYYLWAKHGV PWADD 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC003970 Genomic DNA. Translation: AAC33202.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28440.1.
AY056232 mRNA. Translation: AAL07081.1.
AY117271 mRNA. Translation: AAM51346.1.
PIRE86227.
RefSeqNP_563844.1. NM_100813.2.
UniGeneAt.26355.

3D structure databases

ProteinModelPortalQ93ZW0.
SMRQ93ZW0. Positions 158-622.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid22706. 2 interactions.
STRING3702.AT1G09420.1-P.

Proteomic databases

PaxDbQ93ZW0.
PRIDEQ93ZW0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G09420.1; AT1G09420.1; AT1G09420. [Q93ZW0-1]
GeneID837465.
KEGGath:AT1G09420.

Organism-specific databases

TAIRAT1G09420.

Phylogenomic databases

eggNOGCOG0364.
HOGENOMHOG000046192.
InParanoidQ93ZW0.
KOK00036.
PhylomeDBQ93ZW0.
ProtClustDBCLSN2916991.

Enzyme and pathway databases

BioCycARA:AT1G09420-MONOMER.
ARA:GQT-74-MONOMER.
UniPathwayUPA00115; UER00408.

Gene expression databases

ArrayExpressQ93ZW0.
GenevestigatorQ93ZW0.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_00966. G6PD.
InterProIPR001282. G6P_DH.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23429. PTHR23429. 1 hit.
PfamPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PRINTSPR00079. G6PDHDRGNASE.
TIGRFAMsTIGR00871. zwf. 1 hit.
ProtoNetSearch...

Entry information

Entry nameG6PD4_ARATH
AccessionPrimary (citable) accession number: Q93ZW0
Secondary accession number(s): O80525
Entry history
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names