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Q93ZW0

- G6PD4_ARATH

UniProt

Q93ZW0 - G6PD4_ARATH

Protein

Glucose-6-phosphate 1-dehydrogenase 4, chloroplastic

Gene

At1g09420

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division.

    Catalytic activityi

    D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

    Enzyme regulationi

    Regulated by metabolites. Post-translationally inactivated by cysteine-mediated redox modification via the ferredoxin-thioredoxin system in the light and this avoids futile cycles with photosynthetic CO2 fixation By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei194 – 1941NADPBy similarity
    Binding sitei297 – 2971NADP; via carbonyl oxygenBy similarity
    Binding sitei297 – 2971SubstrateBy similarity
    Binding sitei365 – 3651SubstrateBy similarity
    Binding sitei382 – 3821SubstrateBy similarity
    Active sitei387 – 3871Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi160 – 1678NADPBy similarity

    GO - Molecular functioni

    1. glucose-6-phosphate dehydrogenase activity Source: UniProtKB-EC
    2. NADP binding Source: InterPro

    GO - Biological processi

    1. pentose-phosphate shunt Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciARA:AT1G09420-MONOMER.
    ARA:GQT-74-MONOMER.
    UniPathwayiUPA00115; UER00408.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucose-6-phosphate 1-dehydrogenase 4, chloroplastic (EC:1.1.1.49)
    Short name:
    G6PD4
    Short name:
    G6PDH4
    Gene namesi
    Ordered Locus Names:At1g09420
    ORF Names:F14J9.8
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G09420.

    Subcellular locationi

    Plastidchloroplast By similarity

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4949ChloroplastSequence AnalysisAdd
    BLAST
    Chaini50 – 625576Glucose-6-phosphate 1-dehydrogenase 4, chloroplasticPRO_0000010438Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi212 ↔ 220Redox modulationBy similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiQ93ZW0.
    PRIDEiQ93ZW0.

    Expressioni

    Gene expression databases

    ArrayExpressiQ93ZW0.
    GenevestigatoriQ93ZW0.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi22706. 2 interactions.
    STRINGi3702.AT1G09420.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ93ZW0.
    SMRiQ93ZW0. Positions 158-622.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni327 – 3315Substrate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0364.
    HOGENOMiHOG000046192.
    InParanoidiQ93ZW0.
    KOiK00036.
    PhylomeDBiQ93ZW0.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    HAMAPiMF_00966. G6PD.
    InterProiIPR001282. G6P_DH.
    IPR022675. G6P_DH_C.
    IPR022674. G6P_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23429. PTHR23429. 1 hit.
    PfamiPF02781. G6PD_C. 1 hit.
    PF00479. G6PD_N. 1 hit.
    [Graphical view]
    PRINTSiPR00079. G6PDHDRGNASE.
    TIGRFAMsiTIGR00871. zwf. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q93ZW0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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    MSLSSCLLPF SQSATAPSSS VCSCHLAASF SNFPVSSRDY SFSRSGSLVL    50
    NGGGSNLCRR FCGLKLWILK SLNRRQGNNR KHQPVNELTT HSKHTFLSDD 100
    ERGFAEETRA EDLRPEENIL GTDLNDGFHN VGDLPPVSKQ LSDDLSDVRR 150
    RASLCIAVVG ATGELARGKI FPALFALYYS GYLPEDVAIF GVSRKNLTDE 200
    DLRSIIASTL TCRVDHQENC GGKMDAFQSR TYYINGGYNN RDGMSRLAER 250
    MKQIEGESEA NRIFYLSVPQ EALVDVACTI GDNAQAPRGW TRIIVEKPFG 300
    FNSHSSHQLT KSLLSKFEEK QIYRIDHMLG RNLIENLTVL RFSNLVFEPL 350
    WNRTYIRNIQ VIISESIAQT EKFSDGYGII RDIVHSHILQ TIALLAMEPP 400
    ISLDGEDIRN EKVKVLRSIR KIDPRDVILG QYKSSSRDKN GVILNGVDPT 450
    YCAAALYIDN ARWDGVPFLV RVGTGLIKHR VEIHVQFRHV PGNLYRENIG 500
    INIDLGTNEL ILRDEPDEAI LVKINNKVPG LGLQLDASEL NLLYKDRYKT 550
    EVPDSYEHLI HDVIDGDNHL FMRSDEVAAA WNILSPVLEE IDKHHTAPEL 600
    YEFGGRGPVA AYYLWAKHGV PWADD 625
    Length:625
    Mass (Da):70,202
    Last modified:December 1, 2001 - v1
    Checksum:i83D9F8D96F0B66F4
    GO

    Sequence cautioni

    The sequence AAC33202.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC003970 Genomic DNA. Translation: AAC33202.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE28440.1.
    AY056232 mRNA. Translation: AAL07081.1.
    AY117271 mRNA. Translation: AAM51346.1.
    PIRiE86227.
    RefSeqiNP_563844.1. NM_100813.2. [Q93ZW0-1]
    UniGeneiAt.26355.

    Genome annotation databases

    EnsemblPlantsiAT1G09420.1; AT1G09420.1; AT1G09420. [Q93ZW0-1]
    GeneIDi837465.
    KEGGiath:AT1G09420.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC003970 Genomic DNA. Translation: AAC33202.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE28440.1 .
    AY056232 mRNA. Translation: AAL07081.1 .
    AY117271 mRNA. Translation: AAM51346.1 .
    PIRi E86227.
    RefSeqi NP_563844.1. NM_100813.2. [Q93ZW0-1 ]
    UniGenei At.26355.

    3D structure databases

    ProteinModelPortali Q93ZW0.
    SMRi Q93ZW0. Positions 158-622.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 22706. 2 interactions.
    STRINGi 3702.AT1G09420.1-P.

    Proteomic databases

    PaxDbi Q93ZW0.
    PRIDEi Q93ZW0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G09420.1 ; AT1G09420.1 ; AT1G09420 . [Q93ZW0-1 ]
    GeneIDi 837465.
    KEGGi ath:AT1G09420.

    Organism-specific databases

    TAIRi AT1G09420.

    Phylogenomic databases

    eggNOGi COG0364.
    HOGENOMi HOG000046192.
    InParanoidi Q93ZW0.
    KOi K00036.
    PhylomeDBi Q93ZW0.

    Enzyme and pathway databases

    UniPathwayi UPA00115 ; UER00408 .
    BioCyci ARA:AT1G09420-MONOMER.
    ARA:GQT-74-MONOMER.

    Gene expression databases

    ArrayExpressi Q93ZW0.
    Genevestigatori Q93ZW0.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    HAMAPi MF_00966. G6PD.
    InterProi IPR001282. G6P_DH.
    IPR022675. G6P_DH_C.
    IPR022674. G6P_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23429. PTHR23429. 1 hit.
    Pfami PF02781. G6PD_C. 1 hit.
    PF00479. G6PD_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00079. G6PDHDRGNASE.
    TIGRFAMsi TIGR00871. zwf. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiG6PD4_ARATH
    AccessioniPrimary (citable) accession number: Q93ZW0
    Secondary accession number(s): O80525
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 25, 2002
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3