Reviewed, UniProtKB/Swiss-Prot Q93ZW0 (G6PD4_ARATH)

Names and origin

Protein names

Recommended name:
    Glucose-6-phosphate 1-dehydrogenase 4, chloroplastic
      Short name=G6PDH4
      Short name=G6PD4
    EC=1.1.1.49

Gene names

Ordered Locus Names:	At1g09420
ORF Names:	F14J9.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	625 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division.
Catalytic activity	D-glucose 6-phosphate + NADP+ = D-glucono-1,5-lactone 6-phosphate + NADPH.
Enzyme regulation	Regulated by metabolites. Post-translationally inactivated by cysteine-mediated redox modification via the ferredoxin-thioredoxin system in the light and this avoids futile cycles with photosynthetic CO2 fixation By similarity.
Pathway	Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Subunit structure	Homodimer By similarity.
Subcellular location	Plastid › chloroplast By similarity.
Miscellaneous	There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana.
Sequence similarities	Belongs to the glucose-6-phosphate dehydrogenase family.
Sequence caution	The sequence AAC33202.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro glucose-6-phosphate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 49	49	Chloroplast Potential
	Chain	50 – 625	576	Glucose-6-phosphate 1-dehydrogenase 4, chloroplastic		PRO_0000010438
Sites
	Active site	387	1	Proton acceptor By similarity
	Binding site	162	1	NADP By similarity
	Binding site	194	1	NADP By similarity
	Binding site	327	1	Substrate By similarity
Amino acid modifications
	Disulfide bond	212 ↔ 220		Redox modulation By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q93ZW0-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 83D9F8D96F0B66F4	FASTA	625	70,202
	10 20 30 40 50 60 MSLSSCLLPF SQSATAPSSS VCSCHLAASF SNFPVSSRDY SFSRSGSLVL NGGGSNLCRR 70 80 90 100 110 120 FCGLKLWILK SLNRRQGNNR KHQPVNELTT HSKHTFLSDD ERGFAEETRA EDLRPEENIL 130 140 150 160 170 180 GTDLNDGFHN VGDLPPVSKQ LSDDLSDVRR RASLCIAVVG ATGELARGKI FPALFALYYS 190 200 210 220 230 240 GYLPEDVAIF GVSRKNLTDE DLRSIIASTL TCRVDHQENC GGKMDAFQSR TYYINGGYNN 250 260 270 280 290 300 RDGMSRLAER MKQIEGESEA NRIFYLSVPQ EALVDVACTI GDNAQAPRGW TRIIVEKPFG 310 320 330 340 350 360 FNSHSSHQLT KSLLSKFEEK QIYRIDHMLG RNLIENLTVL RFSNLVFEPL WNRTYIRNIQ 370 380 390 400 410 420 VIISESIAQT EKFSDGYGII RDIVHSHILQ TIALLAMEPP ISLDGEDIRN EKVKVLRSIR 430 440 450 460 470 480 KIDPRDVILG QYKSSSRDKN GVILNGVDPT YCAAALYIDN ARWDGVPFLV RVGTGLIKHR 490 500 510 520 530 540 VEIHVQFRHV PGNLYRENIG INIDLGTNEL ILRDEPDEAI LVKINNKVPG LGLQLDASEL 550 560 570 580 590 600 NLLYKDRYKT EVPDSYEHLI HDVIDGDNHL FMRSDEVAAA WNILSPVLEE IDKHHTAPEL 610 620 YEFGGRGPVA AYYLWAKHGV PWADD

References

[1]

"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.

[2]

"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC003970 Genomic DNA. Translation: AAC33202.1. Sequence problems. AY056232 mRNA. Translation: AAL07081.1. AY117271 mRNA. Translation: AAM51346.1.
IPI	IPI00516947.
PIR	E86227.
RefSeq	NP_563844.1.
UniGene	At.26355
3D structure databases
HSSP	HSSP built from PDB template 1QKI based on UniProtKB P11413.
ModBase	Search...
Proteomic databases
PRIDE	Q93ZW0.
Genome annotation databases
GeneID	837465.
GenomeReviews	Gene locus AT1G09420 in contig CT485782_GR.
KEGG	ath:AT1G09420.
NMPDR	fig|3702.1.peg.1165.
Organism-specific databases
TAIR	At1g09420.
Phylogenomic databases
OMA	Q93ZW0. IVHSHIL.
Enzyme and pathway databases
BRENDA	1.1.1.49. 302.
Gene expression databases
ArrayExpress	Q93ZW0.
GermOnline	AT1G09420. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001282. Glc-6-P_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23429. G6PDH. 1 hit.
Pfam	PF02781. G6PD_C. 1 hit. PF00479. G6PD_N. 1 hit. [Graphical view]
PRINTS	PR00079. G6PDHDRGNASE.
ProDom	PD001129. G6PD. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00871. zwf. 1 hit.
ProtoNet	Search...

Entry information

Entry name

G6PD4_ARATH

Accession

Primary (citable) accession number: Q93ZW0
Secondary accession number(s): O80525

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2002
Last sequence update:	December 1, 2001
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)