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Q93ZW0

- G6PD4_ARATH

UniProt

Q93ZW0 - G6PD4_ARATH

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Protein

Glucose-6-phosphate 1-dehydrogenase 4, chloroplastic

Gene

At1g09420

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division.

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

Enzyme regulationi

Regulated by metabolites. Post-translationally inactivated by cysteine-mediated redox modification via the ferredoxin-thioredoxin system in the light and this avoids futile cycles with photosynthetic CO2 fixation By similarity.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei194 – 1941NADPBy similarity
Binding sitei297 – 2971NADP; via carbonyl oxygenBy similarity
Binding sitei297 – 2971SubstrateBy similarity
Binding sitei365 – 3651SubstrateBy similarity
Binding sitei382 – 3821SubstrateBy similarity
Active sitei387 – 3871Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi160 – 1678NADPBy similarity

GO - Molecular functioni

  1. glucose-6-phosphate dehydrogenase activity Source: UniProtKB-EC
  2. NADP binding Source: InterPro

GO - Biological processi

  1. pentose-phosphate shunt Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciARA:AT1G09420-MONOMER.
ARA:GQT-74-MONOMER.
UniPathwayiUPA00115; UER00408.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenase 4, chloroplastic (EC:1.1.1.49)
Short name:
G6PD4
Short name:
G6PDH4
Gene namesi
Ordered Locus Names:At1g09420
ORF Names:F14J9.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G09420.

Subcellular locationi

Plastidchloroplast By similarity

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4949ChloroplastSequence AnalysisAdd
BLAST
Chaini50 – 625576Glucose-6-phosphate 1-dehydrogenase 4, chloroplasticPRO_0000010438Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi212 ↔ 220Redox modulationBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ93ZW0.
PRIDEiQ93ZW0.

Expressioni

Gene expression databases

ExpressionAtlasiQ93ZW0. baseline and differential.
GenevestigatoriQ93ZW0.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi22706. 2 interactions.
STRINGi3702.AT1G09420.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ93ZW0.
SMRiQ93ZW0. Positions 158-622.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni327 – 3315Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0364.
HOGENOMiHOG000046192.
InParanoidiQ93ZW0.
KOiK00036.
PhylomeDBiQ93ZW0.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD.
InterProiIPR001282. G6P_DH.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PRINTSiPR00079. G6PDHDRGNASE.
TIGRFAMsiTIGR00871. zwf. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q93ZW0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLSSCLLPF SQSATAPSSS VCSCHLAASF SNFPVSSRDY SFSRSGSLVL
60 70 80 90 100
NGGGSNLCRR FCGLKLWILK SLNRRQGNNR KHQPVNELTT HSKHTFLSDD
110 120 130 140 150
ERGFAEETRA EDLRPEENIL GTDLNDGFHN VGDLPPVSKQ LSDDLSDVRR
160 170 180 190 200
RASLCIAVVG ATGELARGKI FPALFALYYS GYLPEDVAIF GVSRKNLTDE
210 220 230 240 250
DLRSIIASTL TCRVDHQENC GGKMDAFQSR TYYINGGYNN RDGMSRLAER
260 270 280 290 300
MKQIEGESEA NRIFYLSVPQ EALVDVACTI GDNAQAPRGW TRIIVEKPFG
310 320 330 340 350
FNSHSSHQLT KSLLSKFEEK QIYRIDHMLG RNLIENLTVL RFSNLVFEPL
360 370 380 390 400
WNRTYIRNIQ VIISESIAQT EKFSDGYGII RDIVHSHILQ TIALLAMEPP
410 420 430 440 450
ISLDGEDIRN EKVKVLRSIR KIDPRDVILG QYKSSSRDKN GVILNGVDPT
460 470 480 490 500
YCAAALYIDN ARWDGVPFLV RVGTGLIKHR VEIHVQFRHV PGNLYRENIG
510 520 530 540 550
INIDLGTNEL ILRDEPDEAI LVKINNKVPG LGLQLDASEL NLLYKDRYKT
560 570 580 590 600
EVPDSYEHLI HDVIDGDNHL FMRSDEVAAA WNILSPVLEE IDKHHTAPEL
610 620
YEFGGRGPVA AYYLWAKHGV PWADD
Length:625
Mass (Da):70,202
Last modified:December 1, 2001 - v1
Checksum:i83D9F8D96F0B66F4
GO

Sequence cautioni

The sequence AAC33202.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC003970 Genomic DNA. Translation: AAC33202.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28440.1.
AY056232 mRNA. Translation: AAL07081.1.
AY117271 mRNA. Translation: AAM51346.1.
PIRiE86227.
RefSeqiNP_563844.1. NM_100813.2. [Q93ZW0-1]
UniGeneiAt.26355.

Genome annotation databases

EnsemblPlantsiAT1G09420.1; AT1G09420.1; AT1G09420. [Q93ZW0-1]
GeneIDi837465.
KEGGiath:AT1G09420.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC003970 Genomic DNA. Translation: AAC33202.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE28440.1 .
AY056232 mRNA. Translation: AAL07081.1 .
AY117271 mRNA. Translation: AAM51346.1 .
PIRi E86227.
RefSeqi NP_563844.1. NM_100813.2. [Q93ZW0-1 ]
UniGenei At.26355.

3D structure databases

ProteinModelPortali Q93ZW0.
SMRi Q93ZW0. Positions 158-622.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 22706. 2 interactions.
STRINGi 3702.AT1G09420.1-P.

Proteomic databases

PaxDbi Q93ZW0.
PRIDEi Q93ZW0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G09420.1 ; AT1G09420.1 ; AT1G09420 . [Q93ZW0-1 ]
GeneIDi 837465.
KEGGi ath:AT1G09420.

Organism-specific databases

TAIRi AT1G09420.

Phylogenomic databases

eggNOGi COG0364.
HOGENOMi HOG000046192.
InParanoidi Q93ZW0.
KOi K00036.
PhylomeDBi Q93ZW0.

Enzyme and pathway databases

UniPathwayi UPA00115 ; UER00408 .
BioCyci ARA:AT1G09420-MONOMER.
ARA:GQT-74-MONOMER.

Gene expression databases

ExpressionAtlasi Q93ZW0. baseline and differential.
Genevestigatori Q93ZW0.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
HAMAPi MF_00966. G6PD.
InterProi IPR001282. G6P_DH.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR23429. PTHR23429. 1 hit.
Pfami PF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view ]
PRINTSi PR00079. G6PDHDRGNASE.
TIGRFAMsi TIGR00871. zwf. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiG6PD4_ARATH
AccessioniPrimary (citable) accession number: Q93ZW0
Secondary accession number(s): O80525
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: December 1, 2001
Last modified: October 29, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3