Q93ZR6 (WSD1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
O-acyltransferase WSD1

Alternative name(s):
Diacylglycerol O-acyltransferase
Short name=DGAT
EC=2.3.1.20
Long-chain-alcohol O-fatty-acyltransferase
EC=2.3.1.75
Wax synthase
Short name=WS

Gene names

Name:	WSD1
Ordered Locus Names:	At5g37300
ORF Names:	MNJ8.9

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Bifunctional wax ester synthase/diacylglycerol acyltransferase. Involved in cuticular wax biosynthesis. Ref.6
Catalytic activity	Acyl-CoA + a long-chain alcohol = CoA + a long-chain ester. Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.
Pathway	Glycerolipid metabolism; triacylglycerol biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein Potential Ref.6.
Tissue specificity	Expressed in flowers, top parts of stems, and leaves. Not found in roots, seeds and young seedlings. Ref.6
Induction	Up-regulated in the stem epidermis during active wax synthesis. Ref.5
Disruption phenotype	Severe reduction in wax alkyl esters. Ref.6
Sequence similarities	In the N-terminal section; belongs to the long-chain O-acyltransferase family.
Sequence caution	The sequence BAB09102.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	wax biosynthetic process Inferred from mutant phenotype Ref.6. Source: TAIR
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	diacylglycerol O-acyltransferase activity Inferred from direct assay Ref.6. Source: TAIR long-chain-alcohol O-fatty-acyltransferase activity Inferred from direct assay Ref.6. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 481	481	O-acyltransferase WSD1		PRO_0000378331
Regions
	Transmembrane	186 – 206	21	Helical; Potential
Sites
	Active site	147	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	Q93ZR6 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: F871EAE6F316E5D6	FASTA	481	54,417
	10 20 30 40 50 60 MKAEKVMERE IETTPIEPLS PMSHMLSSPN FFIVITFGFK TRCNRSAFVD GINNTLINAP 70 80 90 100 110 120 RFSSKMEINY KKKGEPVWIP VKLRVDDHII VPDLEYSNIQ NPDQFVEDYT SNIANIPMDM 130 140 150 160 170 180 SKPLWEFHLL NMKTSKAESL AIVKIHHSIG DGMSLMSLLL ACSRKISDPD ALVSNTTATK 190 200 210 220 230 240 KPADSMAWWL FVGFWFMIRV TFTTIVEFSK LMLTVCFLED TKNPLMGNPS DGFQSWKVVH 250 260 270 280 290 300 RIISFEDVKL IKDTMNMKVN DVLLGMTQAG LSRYLSSKYD GSTAEKKKIL EKLRVRGAVA 310 320 330 340 350 360 INLRPATKIE DLADMMAKGS KCRWGNFIGT VIFPLWVKSE KDPLEYIRRA KATMDRKKIS 370 380 390 400 410 420 LEAFFFYGII KFTLKFFGGK AVEAFGKRIF GHTSLAFSNV KGPDEEISFF HHPISYIAGS 430 440 450 460 470 480 ALVGAQALNI HFISYVDKIV INLAVDTTTI QDPNRLCDDM VEALEIIKSA TQGEIFHKTE V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. XI." Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Cuticular lipid composition, surface structure, and gene expression in Arabidopsis stem epidermis." Suh M.C., Samuels A.L., Jetter R., Kunst L., Pollard M., Ohlrogge J., Beisson F. Plant Physiol. 139:1649-1665(2005) [PubMed: 16299169] [Abstract] Cited for: INDUCTION.
[6]	"Identification of the wax ester synthase/acyl-coenzyme A: diacylglycerol acyltransferase WSD1 required for stem wax ester biosynthesis in Arabidopsis." Li F., Wu X., Lam P., Bird D., Zheng H., Samuels A.L., Jetter R., Kunst L. Plant Physiol. 148:97-107(2008) [PubMed: 18621978] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB017069 Genomic DNA. Translation: BAB09102.1. Different initiation. CP002688 Genomic DNA. Translation: AED94163.1. AY056316 mRNA. Translation: AAL07165.1. BT015776 mRNA. Translation: AAU90066.1.
IPI	IPI00536044.
RefSeq	NP_568547.1. NM_123089.2.
UniGene	At.6994.
3D structure databases
ProteinModelPortal	Q93ZR6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q93ZR6.
Proteomic databases
PRIDE	Q93ZR6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G37300.1; AT5G37300.1; AT5G37300.
GeneID	833704.
GenomeReviews	Gene locus AT5G37300 in contig BA000015_GR.
KEGG	ath:AT5G37300.
Organism-specific databases
TAIR	At5g37300.
Phylogenomic databases
eggNOG	NOG09285.
GeneTree	EPGT00050000007710.
OMA	NIANIPM.
PhylomeDB	Q93ZR6.
ProtClustDB	CLSN2689892.
Gene expression databases
ArrayExpress	Q93ZR6.
Genevestigator	Q93ZR6.
Family and domain databases
InterPro	IPR009721. O-acyltransferase_WSD1_C. IPR004255. O-acyltransferase_WSD1_N. [Graphical view]
KO	K15406.
Pfam	PF06974. DUF1298. 1 hit. PF03007. WES_acyltransf. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

WSD1_ARATH

Accession

Primary (citable) accession number: Q93ZR6
Secondary accession number(s): Q9FHT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	December 1, 2001
Last modified:	December 14, 2011
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program