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Q93ZR1

- ELP3_ARATH

UniProt

Q93ZR1 - ELP3_ARATH

Protein

Elongator complex protein 3

Gene

HAG3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Histone acetyltransferase component of the large multiprotein complex Elongator that is involved in the regulation of transcription initiation and elongation. May also have a methyltransferase activity. Promotes organs development by modulating cell division rate. Required for auxin distribution or signaling. Involved in tRNA wobble uridine modification. Mediates the establishment of leaf polarity independently of AS2 and the ta-siRNA-related pathway.3 Publications

    Catalytic activityi

    Acetyl-CoA + [histone] = CoA + acetyl-[histone].

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi117 – 1171Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi127 – 1271Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi130 – 1301Iron-sulfur (4Fe-4S-S-AdoMet)By similarity

    GO - Molecular functioni

    1. histone acetyltransferase activity Source: UniProtKB-EC
    2. iron-sulfur cluster binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. auxin-activated signaling pathway Source: UniProtKB-KW
    2. cell proliferation Source: TAIR
    3. DNA mediated transformation Source: TAIR
    4. organ growth Source: TAIR
    5. regulation of auxin mediated signaling pathway Source: UniProtKB
    6. regulation of leaf formation Source: UniProtKB
    7. regulation of transcription, DNA-templated Source: UniProtKB-KW
    8. specification of organ axis polarity Source: UniProtKB
    9. transcription, DNA-templated Source: UniProtKB-KW
    10. tRNA wobble uridine modification Source: UniProtKB

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Auxin signaling pathway, Transcription, Transcription regulation, tRNA processing

    Keywords - Ligandi

    Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciARA:AT5G50320-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Elongator complex protein 3 (EC:2.3.1.48)
    Short name:
    AtELP3
    Alternative name(s):
    Elongator component 3
    Protein ELONGATA 3
    Protein ENHANCER-OF-ASYMMETRIC-LEAVES-TWO1
    Gene namesi
    Name:HAG3
    Synonyms:EAST1, ELO3, ELP3
    Ordered Locus Names:At5g50320
    ORF Names:MXI22.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G50320.

    Subcellular locationi

    Nucleus 1 Publication
    Note: Localized in euchromatin during interphase.

    GO - Cellular componenti

    1. cytosol Source: TAIR
    2. Elongator holoenzyme complex Source: UniProtKB
    3. nuclear euchromatin Source: UniProtKB
    4. nucleus Source: TAIR

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Slow growing rate associated with pale-green and downwardly curled narrow leaves and reduced root growth that results from a decreased cell division rate and a reduced apical dominance. Defect in tRNA wobble uridine modification. Filamentous leaves with abaxialized epidermis in AS2 defective plants.4 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi200 – 2001R → W in east1-1; slow growth associated with pale-green and downwardly curled leaves. 1 Publication
    Mutagenesisi331 – 3311D → N in elo3-1; reduced cell proliferation. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 565565Elongator complex protein 3PRO_0000232128Add
    BLAST

    Proteomic databases

    PaxDbiQ93ZR1.
    PRIDEiQ93ZR1.

    Expressioni

    Tissue specificityi

    Expressed in meristematic tissues of in roots, leaves, seedlings, cotyledons, floral buds and shoot apices.2 Publications

    Gene expression databases

    GenevestigatoriQ93ZR1.

    Interactioni

    Subunit structurei

    Component of the RNA polymerase II elongator complex (Elongator) made of at least six subunits, ELP1/ELO2, ELP2, ELP3/ELO3, ELP4/ELO1, ELP5, and ELP6.1 Publication

    Protein-protein interaction databases

    BioGridi20344. 1 interaction.
    IntActiQ93ZR1. 1 interaction.
    STRINGi3702.AT5G50320.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ93ZR1.
    SMRiQ93ZR1. Positions 508-557.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini414 – 565152N-acetyltransferasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ELP3 family.Curated
    Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1243.
    HOGENOMiHOG000227514.
    InParanoidiQ93ZR1.
    KOiK07739.
    OMAiGYKVVSH.
    PhylomeDBiQ93ZR1.

    Family and domain databases

    Gene3Di3.40.630.30. 1 hit.
    3.80.30.20. 1 hit.
    InterProiIPR016181. Acyl_CoA_acyltransferase.
    IPR006638. Elp3/MiaB/NifB.
    IPR000182. GNAT_dom.
    IPR005910. Hist_AcTrfase_ELP3.
    IPR007197. rSAM.
    IPR023404. rSAM_horseshoe.
    [Graphical view]
    PfamiPF00583. Acetyltransf_1. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005669. Hist_AcTrfase_ELP3. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    SUPFAMiSSF55729. SSF55729. 1 hit.
    TIGRFAMsiTIGR01211. ELP3. 1 hit.
    PROSITEiPS51186. GNAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q93ZR1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATAVVMNGE LKKQPRPGKG GYQGRGLTEE EARVRAISEI VSTMIERSHR    50
    NENVDLNAIK TAACRKYGLA RAPKLVEMIA ALPDSERETL LPKLRAKPVR 100
    TASGIAVVAV MSKPHRCPHI ATTGNICVYC PGGPDSDFEY STQSYTGYEP 150
    TSMRAIRARY NPYVQARSRI DQLKRLGHSV DKVEFILMGG TFMSLPAEYR 200
    DFFIRNLHDA LSGHTSANVE EAVAYSEHSA TKCIGMTIET RPDYCLGPHL 250
    RQMLIYGCTR LEIGVQSTYE DVARDTNRGH TVAAVADCFC LAKDAGFKVV 300
    AHMMPDLPNV GVERDMESFK EFFESPSFRA DGLKIYPTLV IRGTGLYELW 350
    KTGRYRNYPP EQLVDIVARI LSMVPPWTRV YRVQRDIPMP LVTSGVEKGN 400
    LRELALARMD DLGLKCRDVR TREAGIQDIH HKIKPEQVEL VRRDYTANEG 450
    WETFLSYEDT RQDILVGLLR LRKCGKNVTC PELMGKCSVV RELHVYGTAV 500
    PVHGRDADKL QHQGYGTLLM EEAERIARRE HRSNKIGVIS GVGTRHYYRK 550
    LGYELEGPYM VKHLL 565
    Length:565
    Mass (Da):63,694
    Last modified:December 1, 2001 - v1
    Checksum:i8F993E4D41CF4B05
    GO

    Sequence cautioni

    The sequence AAL06951.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ964958 mRNA. Translation: CAI79647.1.
    AB012248 Genomic DNA. Translation: BAB09451.1.
    CP002688 Genomic DNA. Translation: AED95925.1.
    AY054293 mRNA. Translation: AAL06951.1. Different initiation.
    AY056323 mRNA. Translation: AAL07172.1.
    AY133528 mRNA. Translation: AAM91358.1.
    RefSeqiNP_568725.1. NM_124412.2.
    UniGeneiAt.20657.

    Genome annotation databases

    EnsemblPlantsiAT5G50320.1; AT5G50320.1; AT5G50320.
    GeneIDi835098.
    KEGGiath:AT5G50320.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ964958 mRNA. Translation: CAI79647.1 .
    AB012248 Genomic DNA. Translation: BAB09451.1 .
    CP002688 Genomic DNA. Translation: AED95925.1 .
    AY054293 mRNA. Translation: AAL06951.1 . Different initiation.
    AY056323 mRNA. Translation: AAL07172.1 .
    AY133528 mRNA. Translation: AAM91358.1 .
    RefSeqi NP_568725.1. NM_124412.2.
    UniGenei At.20657.

    3D structure databases

    ProteinModelPortali Q93ZR1.
    SMRi Q93ZR1. Positions 508-557.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 20344. 1 interaction.
    IntActi Q93ZR1. 1 interaction.
    STRINGi 3702.AT5G50320.1-P.

    Proteomic databases

    PaxDbi Q93ZR1.
    PRIDEi Q93ZR1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G50320.1 ; AT5G50320.1 ; AT5G50320 .
    GeneIDi 835098.
    KEGGi ath:AT5G50320.

    Organism-specific databases

    TAIRi AT5G50320.

    Phylogenomic databases

    eggNOGi COG1243.
    HOGENOMi HOG000227514.
    InParanoidi Q93ZR1.
    KOi K07739.
    OMAi GYKVVSH.
    PhylomeDBi Q93ZR1.

    Enzyme and pathway databases

    BioCyci ARA:AT5G50320-MONOMER.

    Miscellaneous databases

    PROi Q93ZR1.

    Gene expression databases

    Genevestigatori Q93ZR1.

    Family and domain databases

    Gene3Di 3.40.630.30. 1 hit.
    3.80.30.20. 1 hit.
    InterProi IPR016181. Acyl_CoA_acyltransferase.
    IPR006638. Elp3/MiaB/NifB.
    IPR000182. GNAT_dom.
    IPR005910. Hist_AcTrfase_ELP3.
    IPR007197. rSAM.
    IPR023404. rSAM_horseshoe.
    [Graphical view ]
    Pfami PF00583. Acetyltransf_1. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005669. Hist_AcTrfase_ELP3. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55729. SSF55729. 1 hit.
    TIGRFAMsi TIGR01211. ELP3. 1 hit.
    PROSITEi PS51186. GNAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The elongata mutants identify a functional Elongator complex in plants with a role in cell proliferation during organ growth."
      Nelissen H., Fleury D., Bruno L., Robles P., de Veylder L., Traas J., Micol J., Van Montagu M., Inze D., Van Lijsebettens M.
      Proc. Natl. Acad. Sci. U.S.A. 102:7754-7759(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF ASP-331, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
      Strain: cv. Landsberg erecta.
      Tissue: Seedling.
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
      Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
      DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Analysis of histone acetyltransferase and histone deacetylase families of Arabidopsis thaliana suggests functional diversification of chromatin modification among multicellular eukaryotes."
      Pandey R., Mueller A., Napoli C.A., Selinger D.A., Pikaard C.S., Richards E.J., Bender J., Mount D.W., Jorgensen R.A.
      Nucleic Acids Res. 30:5036-5055(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE.
    6. "Elongator function in tRNA wobble uridine modification is conserved between yeast and plants."
      Mehlgarten C., Jablonowski D., Wrackmeyer U., Tschitschmann S., Sondermann D., Jaeger G., Gong Z., Bystroem A.S., Schaffrath R., Breunig K.D.
      Mol. Microbiol. 76:1082-1094(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    7. Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    8. "Asymmetric leaves2 and Elongator, a histone acetyltransferase complex, mediate the establishment of polarity in leaves of Arabidopsis thaliana."
      Kojima S., Iwasaki M., Takahashi H., Imai T., Matsumura Y., Fleury D., Van Lijsebettens M., Machida Y., Machida C.
      Plant Cell Physiol. 52:1259-1273(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF ARG-200, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiELP3_ARATH
    AccessioniPrimary (citable) accession number: Q93ZR1
    Secondary accession number(s): Q9FK40
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 4, 2006
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3