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Protein

Elongator complex protein 3

Gene

HAG3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Histone acetyltransferase component of the large multiprotein complex Elongator that is involved in the regulation of transcription initiation and elongation (PubMed:20080602). May also have a methyltransferase activity (PubMed:20080602). Promotes organs development by modulating cell division rate (PubMed:20080602). Required for auxin distribution or signaling (PubMed:20080602). Required for meristem cell cycle activation at the time of germination (PubMed:23969312). Probably required for DNA replication-coupled histone acetylation (PubMed:22026817). Involved in tRNA wobble uridine modification (PubMed:20398216). Mediates the establishment of leaf polarity independently of AS2 and the ta-siRNA-related pathway (PubMed:21700721). Potential a-tubulin acetyltransferase (PubMed:22404109). Negatively regulates the expression of genes that participate in UV-B responses, such as DNA repair enzymes and enzymes that participate in sunscreen pigment biosynthesis (PubMed:25907565).1 Publication6 Publications

Catalytic activityi

Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine.Curated

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi117Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi127Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi130Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1

GO - Molecular functioni

GO - Biological processi

  • auxin-activated signaling pathway Source: UniProtKB-KW
  • cell proliferation Source: TAIR
  • DNA mediated transformation Source: TAIR
  • organ growth Source: TAIR
  • regulation of auxin mediated signaling pathway Source: UniProtKB
  • regulation of leaf formation Source: UniProtKB
  • regulation of transcription by RNA polymerase II Source: GO_Central
  • specification of plant organ axis polarity Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
  • tRNA wobble uridine modification Source: UniProtKB

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processAuxin signaling pathway, Transcription, Transcription regulation, tRNA processing
LigandIron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.3.1.48 399

Names & Taxonomyi

Protein namesi
Recommended name:
Elongator complex protein 31 Publication (EC:2.3.1.48Curated)
Short name:
AtELP31 Publication
Alternative name(s):
Elongator component 31 Publication
Protein ELONGATA 31 Publication
Protein ENHANCER-OF-ASYMMETRIC-LEAVES-TWO11 Publication
Gene namesi
Name:HAG31 Publication
Synonyms:EAST11 Publication, ELO31 Publication, ELP31 Publication
Ordered Locus Names:At5g50320Imported
ORF Names:MXI22.3Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G50320
TAIRilocus:2177477 AT5G50320

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Slow growing rate associated with pale-green and downwardly curled narrow leaves and reduced root growth that results from a decreased cell division rate and a reduced apical dominance. Defect in tRNA wobble uridine modification. Filamentous leaves with abaxialized epidermis in AS2 defective plants.4 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi200R → W in east1-1; slow growth associated with pale-green and downwardly curled leaves. 1 Publication1
Mutagenesisi331D → N in elo3-1; reduced cell proliferation. 1 Publication1
Mutagenesisi423E → K in elo3-14; reduced tissue proliferation in the meristem. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002321281 – 565Elongator complex protein 3Add BLAST565

Proteomic databases

PaxDbiQ93ZR1

PTM databases

iPTMnetiQ93ZR1

Expressioni

Tissue specificityi

Expressed in meristematic tissues of in roots, leaves, seedlings, cotyledons, floral buds and shoot apices (PubMed:15894610, PubMed:20080602, PubMed:23969312). Expressed in the shoot meristem and the emerging leaves immediately after germination (PubMed:23969312). In the root, expressed in the transition zone and the upper meristematic zone (PubMed:23969312).3 Publications

Gene expression databases

ExpressionAtlasiQ93ZR1 baseline and differential
GenevisibleiQ93ZR1 AT

Interactioni

Subunit structurei

Component of the RNA polymerase II elongator complex (Elongator) made of at least six subunits, ELP1/ELO2, ELP2, ELP3/ELO3, ELP4/ELO1, ELP5, and ELP6 (PubMed:20080602). Interacts with IYO (PubMed:21620701).2 Publications

Protein-protein interaction databases

BioGridi20344, 1 interactor
IntActiQ93ZR1, 3 interactors
STRINGi3702.AT5G50320.1

Structurei

3D structure databases

ProteinModelPortaliQ93ZR1
SMRiQ93ZR1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini414 – 565N-acetyltransferasePROSITE-ProRule annotationAdd BLAST152

Sequence similaritiesi

Belongs to the ELP3 family.Curated

Phylogenomic databases

eggNOGiKOG2535 Eukaryota
COG1243 LUCA
HOGENOMiHOG000227514
InParanoidiQ93ZR1
KOiK07739
OMAiSYTGKEP
OrthoDBiEOG093605YI
PhylomeDBiQ93ZR1

Family and domain databases

Gene3Di3.80.30.20, 1 hit
InterProiView protein in InterPro
IPR016181 Acyl_CoA_acyltransferase
IPR034687 ELP3-like
IPR006638 Elp3/MiaB/NifB
IPR000182 GNAT_dom
IPR032432 Radical_SAM_C
IPR007197 rSAM
IPR023404 rSAM_horseshoe
PANTHERiPTHR11135:SF0 PTHR11135:SF0, 1 hit
PfamiView protein in Pfam
PF00583 Acetyltransf_1, 1 hit
PF04055 Radical_SAM, 1 hit
PF16199 Radical_SAM_C, 1 hit
PIRSFiPIRSF005669 Hist_AcTrfase_ELP3, 1 hit
SFLDiSFLDF00344 ELP3-like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
SUPFAMiSSF55729 SSF55729, 1 hit
TIGRFAMsiTIGR01211 ELP3, 1 hit
PROSITEiView protein in PROSITE
PS51186 GNAT, 1 hit

Sequencei

Sequence statusi: Complete.

Q93ZR1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATAVVMNGE LKKQPRPGKG GYQGRGLTEE EARVRAISEI VSTMIERSHR
60 70 80 90 100
NENVDLNAIK TAACRKYGLA RAPKLVEMIA ALPDSERETL LPKLRAKPVR
110 120 130 140 150
TASGIAVVAV MSKPHRCPHI ATTGNICVYC PGGPDSDFEY STQSYTGYEP
160 170 180 190 200
TSMRAIRARY NPYVQARSRI DQLKRLGHSV DKVEFILMGG TFMSLPAEYR
210 220 230 240 250
DFFIRNLHDA LSGHTSANVE EAVAYSEHSA TKCIGMTIET RPDYCLGPHL
260 270 280 290 300
RQMLIYGCTR LEIGVQSTYE DVARDTNRGH TVAAVADCFC LAKDAGFKVV
310 320 330 340 350
AHMMPDLPNV GVERDMESFK EFFESPSFRA DGLKIYPTLV IRGTGLYELW
360 370 380 390 400
KTGRYRNYPP EQLVDIVARI LSMVPPWTRV YRVQRDIPMP LVTSGVEKGN
410 420 430 440 450
LRELALARMD DLGLKCRDVR TREAGIQDIH HKIKPEQVEL VRRDYTANEG
460 470 480 490 500
WETFLSYEDT RQDILVGLLR LRKCGKNVTC PELMGKCSVV RELHVYGTAV
510 520 530 540 550
PVHGRDADKL QHQGYGTLLM EEAERIARRE HRSNKIGVIS GVGTRHYYRK
560
LGYELEGPYM VKHLL
Length:565
Mass (Da):63,694
Last modified:December 1, 2001 - v1
Checksum:i8F993E4D41CF4B05
GO

Sequence cautioni

The sequence AAL06951 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ964958 mRNA Translation: CAI79647.1
AB012248 Genomic DNA Translation: BAB09451.1
CP002688 Genomic DNA Translation: AED95925.1
AY054293 mRNA Translation: AAL06951.1 Different initiation.
AY056323 mRNA Translation: AAL07172.1
AY133528 mRNA Translation: AAM91358.1
RefSeqiNP_568725.1, NM_124412.3
UniGeneiAt.20657

Genome annotation databases

EnsemblPlantsiAT5G50320.1; AT5G50320.1; AT5G50320
GeneIDi835098
GrameneiAT5G50320.1; AT5G50320.1; AT5G50320
KEGGiath:AT5G50320

Similar proteinsi

Entry informationi

Entry nameiELP3_ARATH
AccessioniPrimary (citable) accession number: Q93ZR1
Secondary accession number(s): Q9FK40
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: December 1, 2001
Last modified: May 23, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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