Reviewed, UniProtKB/Swiss-Prot Q93ZR1 (ELP3_ARATH)

Names and origin

Protein names

Recommended name:
    Elongator complex protein 3
    EC=2.3.1.48
Alternative name(s):
    Protein ELONGATA 3
    Elongator component 3

Gene names

Name:	HAG3
Synonyms:	ELO3, ELP3
Ordered Locus Names:	At5g50320
ORF Names:	MXI22.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Histone acetyltransferase By similarity. Part of the large multiprotein complex Elongator that is involved in the regulation of transcription initiation and elongation. May also have a methyltransferase activity.
Catalytic activity	Acetyl-CoA + histone = CoA + acetylhistone.
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Subunit structure	Part of the Elongator core subcomplex.
Subcellular location	Nucleus Probable.
Tissue specificity	Expressed in roots, leaves, seedlings, cotyledons, floral buds and shoot apices. Ref.1
Sequence similarities	Belongs to the ELP3 family. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA mediated transformation Inferred from mutant phenotype. Source: TAIR cell proliferation Ref.1 Inferred from mutant phenotype. Source: TAIR organ growth Ref.1 Inferred from mutant phenotype. Source: TAIR regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	histone acetyltransferase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW iron-sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	Elongator complex protein 3		PRO_0000232128
Regions
	Domain	414 – 565	152	N-acetyltransferase
Sites
	Metal binding	117	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	127	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	130	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Experimental info
	Mutagenesis	331	1	D → N in elo3-1; reduced cell proliferation. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q93ZR1-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 8F993E4D41CF4B05	FASTA	565	63,694
	10 20 30 40 50 60 MATAVVMNGE LKKQPRPGKG GYQGRGLTEE EARVRAISEI VSTMIERSHR NENVDLNAIK 70 80 90 100 110 120 TAACRKYGLA RAPKLVEMIA ALPDSERETL LPKLRAKPVR TASGIAVVAV MSKPHRCPHI 130 140 150 160 170 180 ATTGNICVYC PGGPDSDFEY STQSYTGYEP TSMRAIRARY NPYVQARSRI DQLKRLGHSV 190 200 210 220 230 240 DKVEFILMGG TFMSLPAEYR DFFIRNLHDA LSGHTSANVE EAVAYSEHSA TKCIGMTIET 250 260 270 280 290 300 RPDYCLGPHL RQMLIYGCTR LEIGVQSTYE DVARDTNRGH TVAAVADCFC LAKDAGFKVV 310 320 330 340 350 360 AHMMPDLPNV GVERDMESFK EFFESPSFRA DGLKIYPTLV IRGTGLYELW KTGRYRNYPP 370 380 390 400 410 420 EQLVDIVARI LSMVPPWTRV YRVQRDIPMP LVTSGVEKGN LRELALARMD DLGLKCRDVR 430 440 450 460 470 480 TREAGIQDIH HKIKPEQVEL VRRDYTANEG WETFLSYEDT RQDILVGLLR LRKCGKNVTC 490 500 510 520 530 540 PELMGKCSVV RELHVYGTAV PVHGRDADKL QHQGYGTLLM EEAERIARRE HRSNKIGVIS 550 560 GVGTRHYYRK LGYELEGPYM VKHLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The elongata mutants identify a functional Elongator complex in plants with a role in cell proliferation during organ growth." Nelissen H., Fleury D., Bruno L., Robles P., de Veylder L., Traas J., Micol J., Van Montagu M., Inze D., Van Lijsebettens M. Proc. Natl. Acad. Sci. U.S.A. 102:7754-7759(2005) [PubMed: 15894610] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF ASP-331, TISSUE SPECIFICITY. Strain: cv. Landsberg erecta. Tissue: Seedling.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones." Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S. DNA Res. 5:203-216(1998) [PubMed: 9734815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Analysis of histone acetyltransferase and histone deacetylase families of Arabidopsis thaliana suggests functional diversification of chromatin modification among multicellular eukaryotes." Pandey R., Mueller A., Napoli C.A., Selinger D.A., Pikaard C.S., Richards E.J., Bender J., Mount D.W., Jorgensen R.A. Nucleic Acids Res. 30:5036-5055(2002) [PubMed: 12466527] [Abstract] Cited for: NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ964958 mRNA. Translation: CAI79647.1. AB012248 Genomic DNA. Translation: BAB09451.1. AY054293 mRNA. Translation: AAL06951.1. Different initiation. AY056323 mRNA. Translation: AAL07172.1. AY133528 mRNA. Translation: AAM91358.1.
IPI	IPI00539057.
RefSeq	NP_568725.1.
UniGene	At.20657
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q93ZR1.
Proteomic databases
PRIDE	Q93ZR1.
Genome annotation databases
GeneID	835098.
GenomeReviews	Gene locus AT5G50320 in contig BA000015_GR.
KEGG	ath:AT5G50320.
NMPDR	fig|3702.1.peg.26886.
Organism-specific databases
TAIR	At5g50320.
Phylogenomic databases
OMA	WQHRGYG.
Enzyme and pathway databases
BRENDA	2.3.1.48. 302.
Gene expression databases
ArrayExpress	Q93ZR1.
Genevestigator	Q93ZR1.
GermOnline	AT5G50320. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR006638. Elp3/MiaB/NifB. IPR000182. GCN5-rel_AcTrfase. IPR005910. Hist_AcTrfase_ELP3. IPR007197. Radical_SAM. [Graphical view]
Pfam	PF00583. Acetyltransf_1. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF005669. Hist_AcTrfase_ELP3. 1 hit.
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR01211. ELP3. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ELP3_ARATH

Accession

Primary (citable) accession number: Q93ZR1
Secondary accession number(s): Q9FK40

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	December 1, 2001
Last modified:	November 3, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)