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Protein

Probable N-acetyl-gamma-glutamyl-phosphate reductase, chloroplastic

Gene

At2g19940

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate = N-acetyl-5-glutamyl phosphate + NADPH.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Arginine biosynthesis bifunctional protein ArgJ, chloroplastic (AXX17_At2g34300), Probable amino-acid acetyltransferase NAGS2, chloroplastic (NAGS2), Arginine biosynthesis bifunctional protein ArgJ, chloroplastic (At2g37500), Probable amino-acid acetyltransferase NAGS1, chloroplastic (NAGS1)
  2. Acetylglutamate kinase, chloroplastic (NAGK)
  3. Probable N-acetyl-gamma-glutamyl-phosphate reductase, chloroplastic (At2g19940)
  4. Acetylornithine aminotransferase, chloroplastic/mitochondrial (WIN1)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei205By similarity1

GO - Molecular functioni

GO - Biological processi

  • arginine biosynthetic process Source: UniProtKB-UniPathway
  • response to cadmium ion Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciARA:AT2G19940-MONOMER.
UniPathwayiUPA00068; UER00108.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable N-acetyl-gamma-glutamyl-phosphate reductase, chloroplastic (EC:1.2.1.38)
Short name:
AGPR
Alternative name(s):
N-acetyl-glutamate semialdehyde dehydrogenase
Short name:
NAGSA dehydrogenase
Gene namesi
Ordered Locus Names:At2g19940
ORF Names:F6F22.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G19940.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
  • membrane Source: TAIR
  • nucleolus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 48ChloroplastSequence analysisAdd BLAST48
ChainiPRO_000000206649 – 401Probable N-acetyl-gamma-glutamyl-phosphate reductase, chloroplasticAdd BLAST353

Proteomic databases

PaxDbiQ93Z70.
PRIDEiQ93Z70.

Expressioni

Gene expression databases

ExpressionAtlasiQ93Z70. baseline and differential.
GenevisibleiQ93Z70. AT.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

BioGridi1868. 1 interactor.
IntActiQ93Z70. 1 interactor.
STRINGi3702.AT2G19940.1.

Structurei

Secondary structure

1401
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi59 – 64Combined sources6
Helixi69 – 79Combined sources11
Beta strandi82 – 90Combined sources9
Turni93 – 96Combined sources4
Helixi99 – 102Combined sources4
Helixi104 – 106Combined sources3
Helixi117 – 119Combined sources3
Helixi122 – 124Combined sources3
Beta strandi126 – 130Combined sources5
Turni134 – 136Combined sources3
Helixi137 – 142Combined sources6
Beta strandi149 – 152Combined sources4
Turni156 – 158Combined sources3
Helixi162 – 169Combined sources8
Helixi176 – 179Combined sources4
Helixi187 – 195Combined sources9
Beta strandi198 – 201Combined sources4
Helixi205 – 219Combined sources15
Beta strandi225 – 227Combined sources3
Beta strandi229 – 235Combined sources7
Helixi236 – 239Combined sources4
Helixi245 – 247Combined sources3
Helixi249 – 252Combined sources4
Helixi266 – 278Combined sources13
Beta strandi285 – 287Combined sources3
Beta strandi289 – 295Combined sources7
Beta strandi297 – 305Combined sources9
Helixi311 – 322Combined sources12
Beta strandi326 – 330Combined sources5
Helixi339 – 341Combined sources3
Turni342 – 344Combined sources3
Beta strandi348 – 354Combined sources7
Beta strandi360 – 367Combined sources8
Turni369 – 374Combined sources6
Helixi375 – 385Combined sources11
Turni390 – 393Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XYGX-ray2.19A/B/C/D43-401[»]
2Q49X-ray2.19A/B/C/D43-401[»]
ProteinModelPortaliQ93Z70.
SMRiQ93Z70.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ93Z70.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG4354. Eukaryota.
COG0002. LUCA.
InParanoidiQ93Z70.
KOiK00145.
OMAiTFVPHLT.
OrthoDBiEOG09360B0T.
PhylomeDBiQ93Z70.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00150. ArgC_type1. 1 hit.
InterProiIPR023013. AGPR_AS.
IPR000706. AGPR_type-1.
IPR016040. NAD(P)-bd_dom.
IPR000534. Semialdehyde_DH_NAD-bd.
IPR012280. Semialdhyde_DH_dimer_dom.
[Graphical view]
PfamiPF01118. Semialdhyde_dh. 1 hit.
PF02774. Semialdhyde_dhC. 1 hit.
[Graphical view]
SMARTiSM00859. Semialdhyde_dh. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01850. argC. 1 hit.
PROSITEiPS01224. ARGC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q93Z70-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTASAFSSI QGCWFKGERK IRVADKRAKR LTLGSHVASP SSMSFRVSAS
60 70 80 90 100
SSVKPEKDIR IGLLGASGYT GAEIVRLLAN HPHFQVTLMT ADRKAGQSME
110 120 130 140 150
SVFPHLRAQK LPTLVSVKDA DFSTVDAVFC CLPHGTTQEI IKELPTALKI
160 170 180 190 200
VDLSADFRLR NIAEYEEWYG QPHKAVELQK EVVYGLTEIL REDIKKARLV
210 220 230 240 250
ANPGCYPTTI QLPLVPLLKA NLIKHENIII DAKSGVSGAG RGAKEANLYS
260 270 280 290 300
EIAEGISSYG VTRHRHVPEI EQGLSDVAQS KVTVSFTPHL MPMIRGMQST
310 320 330 340 350
IYVEMAPGVR TEDLHQQLKT SYEDEEFVKV LDEGVVPRTH NVRGSNYCHM
360 370 380 390 400
SVFPDRIPGR AIIISVIDNL VKGASGQALQ NLNIMLGYPE TTGLLHQPLF

P
Length:401
Mass (Da):44,136
Last modified:August 30, 2005 - v2
Checksum:i70A1CFECD1EAD162
GO

Sequence cautioni

The sequence AAC62122 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005169 Genomic DNA. Translation: AAC62122.2. Sequence problems.
CP002685 Genomic DNA. Translation: AEC06945.1.
CP002685 Genomic DNA. Translation: AEC06946.1.
AY058082 mRNA. Translation: AAL24190.1.
AY090309 mRNA. Translation: AAL90970.1.
PIRiA84583.
RefSeqiNP_565461.5. NM_127552.8.
NP_849993.5. NM_179662.6.
UniGeneiAt.21379.
At.38263.
At.74911.

Genome annotation databases

EnsemblPlantsiAT2G19940.1; AT2G19940.1; AT2G19940.
AT2G19940.2; AT2G19940.2; AT2G19940.
GeneIDi816513.
GrameneiAT2G19940.1; AT2G19940.1; AT2G19940.
AT2G19940.2; AT2G19940.2; AT2G19940.
KEGGiath:AT2G19940.
ath:AT2G31100.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005169 Genomic DNA. Translation: AAC62122.2. Sequence problems.
CP002685 Genomic DNA. Translation: AEC06945.1.
CP002685 Genomic DNA. Translation: AEC06946.1.
AY058082 mRNA. Translation: AAL24190.1.
AY090309 mRNA. Translation: AAL90970.1.
PIRiA84583.
RefSeqiNP_565461.5. NM_127552.8.
NP_849993.5. NM_179662.6.
UniGeneiAt.21379.
At.38263.
At.74911.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XYGX-ray2.19A/B/C/D43-401[»]
2Q49X-ray2.19A/B/C/D43-401[»]
ProteinModelPortaliQ93Z70.
SMRiQ93Z70.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi1868. 1 interactor.
IntActiQ93Z70. 1 interactor.
STRINGi3702.AT2G19940.1.

Proteomic databases

PaxDbiQ93Z70.
PRIDEiQ93Z70.

Protocols and materials databases

DNASUi816513.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G19940.1; AT2G19940.1; AT2G19940.
AT2G19940.2; AT2G19940.2; AT2G19940.
GeneIDi816513.
GrameneiAT2G19940.1; AT2G19940.1; AT2G19940.
AT2G19940.2; AT2G19940.2; AT2G19940.
KEGGiath:AT2G19940.
ath:AT2G31100.

Organism-specific databases

TAIRiAT2G19940.

Phylogenomic databases

eggNOGiKOG4354. Eukaryota.
COG0002. LUCA.
InParanoidiQ93Z70.
KOiK00145.
OMAiTFVPHLT.
OrthoDBiEOG09360B0T.
PhylomeDBiQ93Z70.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00108.
BioCyciARA:AT2G19940-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ93Z70.
PROiQ93Z70.

Gene expression databases

ExpressionAtlasiQ93Z70. baseline and differential.
GenevisibleiQ93Z70. AT.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00150. ArgC_type1. 1 hit.
InterProiIPR023013. AGPR_AS.
IPR000706. AGPR_type-1.
IPR016040. NAD(P)-bd_dom.
IPR000534. Semialdehyde_DH_NAD-bd.
IPR012280. Semialdhyde_DH_dimer_dom.
[Graphical view]
PfamiPF01118. Semialdhyde_dh. 1 hit.
PF02774. Semialdhyde_dhC. 1 hit.
[Graphical view]
SMARTiSM00859. Semialdhyde_dh. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01850. argC. 1 hit.
PROSITEiPS01224. ARGC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARGC_ARATH
AccessioniPrimary (citable) accession number: Q93Z70
Secondary accession number(s): O82187
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: August 30, 2005
Last modified: November 30, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.