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Protein

Probable N-acetyl-gamma-glutamyl-phosphate reductase, chloroplastic

Gene

At2g19940

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate = N-acetyl-5-glutamyl phosphate + NADPH.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Probable amino-acid acetyltransferase NAGS2, chloroplastic (NAGS2), Arginine biosynthesis bifunctional protein ArgJ, chloroplastic (At2g37500), Probable amino-acid acetyltransferase NAGS1, chloroplastic (NAGS1)
  2. Acetylglutamate kinase, chloroplastic (NAGK)
  3. Probable N-acetyl-gamma-glutamyl-phosphate reductase, chloroplastic (At2g19940)
  4. Acetylornithine aminotransferase, chloroplastic/mitochondrial (WIN1)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei205 – 2051By similarity

GO - Molecular functioni

GO - Biological processi

  • arginine biosynthetic process Source: UniProtKB-UniPathway
  • response to cadmium ion Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciARA:AT2G19940-MONOMER.
ARA:GQT-2201-MONOMER.
UniPathwayiUPA00068; UER00108.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable N-acetyl-gamma-glutamyl-phosphate reductase, chloroplastic (EC:1.2.1.38)
Short name:
AGPR
Alternative name(s):
N-acetyl-glutamate semialdehyde dehydrogenase
Short name:
NAGSA dehydrogenase
Gene namesi
Ordered Locus Names:At2g19940
ORF Names:F6F22.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G19940.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
  • membrane Source: TAIR
  • nucleolus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4848ChloroplastSequence analysisAdd
BLAST
Chaini49 – 401353Probable N-acetyl-gamma-glutamyl-phosphate reductase, chloroplasticPRO_0000002066Add
BLAST

Proteomic databases

PaxDbiQ93Z70.
PRIDEiQ93Z70.

Expressioni

Gene expression databases

ExpressionAtlasiQ93Z70. baseline and differential.
GenevisibleiQ93Z70. AT.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

IntActiQ93Z70. 1 interaction.
STRINGi3702.AT2G19940.1.

Structurei

Secondary structure

1
401
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi59 – 646Combined sources
Helixi69 – 7911Combined sources
Beta strandi82 – 909Combined sources
Turni93 – 964Combined sources
Helixi99 – 1024Combined sources
Helixi104 – 1063Combined sources
Helixi117 – 1193Combined sources
Helixi122 – 1243Combined sources
Beta strandi126 – 1305Combined sources
Turni134 – 1363Combined sources
Helixi137 – 1426Combined sources
Beta strandi149 – 1524Combined sources
Turni156 – 1583Combined sources
Helixi162 – 1698Combined sources
Helixi176 – 1794Combined sources
Helixi187 – 1959Combined sources
Beta strandi198 – 2014Combined sources
Helixi205 – 21915Combined sources
Beta strandi225 – 2273Combined sources
Beta strandi229 – 2357Combined sources
Helixi236 – 2394Combined sources
Helixi245 – 2473Combined sources
Helixi249 – 2524Combined sources
Helixi266 – 27813Combined sources
Beta strandi285 – 2873Combined sources
Beta strandi289 – 2957Combined sources
Beta strandi297 – 3059Combined sources
Helixi311 – 32212Combined sources
Beta strandi326 – 3305Combined sources
Helixi339 – 3413Combined sources
Turni342 – 3443Combined sources
Beta strandi348 – 3547Combined sources
Beta strandi360 – 3678Combined sources
Turni369 – 3746Combined sources
Helixi375 – 38511Combined sources
Turni390 – 3934Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XYGX-ray2.19A/B/C/D43-401[»]
2Q49X-ray2.19A/B/C/D43-401[»]
ProteinModelPortaliQ93Z70.
SMRiQ93Z70. Positions 57-401.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ93Z70.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG4354. Eukaryota.
COG0002. LUCA.
InParanoidiQ93Z70.
KOiK00145.
OMAiTFVPHLT.
OrthoDBiEOG09360B0T.
PhylomeDBiQ93Z70.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00150. ArgC_type1. 1 hit.
InterProiIPR023013. AGPR_AS.
IPR000706. AGPR_type-1.
IPR016040. NAD(P)-bd_dom.
IPR000534. Semialdehyde_DH_NAD-bd.
IPR012280. Semialdhyde_DH_dimer_dom.
[Graphical view]
PfamiPF01118. Semialdhyde_dh. 1 hit.
PF02774. Semialdhyde_dhC. 1 hit.
[Graphical view]
SMARTiSM00859. Semialdhyde_dh. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01850. argC. 1 hit.
PROSITEiPS01224. ARGC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q93Z70-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTASAFSSI QGCWFKGERK IRVADKRAKR LTLGSHVASP SSMSFRVSAS
60 70 80 90 100
SSVKPEKDIR IGLLGASGYT GAEIVRLLAN HPHFQVTLMT ADRKAGQSME
110 120 130 140 150
SVFPHLRAQK LPTLVSVKDA DFSTVDAVFC CLPHGTTQEI IKELPTALKI
160 170 180 190 200
VDLSADFRLR NIAEYEEWYG QPHKAVELQK EVVYGLTEIL REDIKKARLV
210 220 230 240 250
ANPGCYPTTI QLPLVPLLKA NLIKHENIII DAKSGVSGAG RGAKEANLYS
260 270 280 290 300
EIAEGISSYG VTRHRHVPEI EQGLSDVAQS KVTVSFTPHL MPMIRGMQST
310 320 330 340 350
IYVEMAPGVR TEDLHQQLKT SYEDEEFVKV LDEGVVPRTH NVRGSNYCHM
360 370 380 390 400
SVFPDRIPGR AIIISVIDNL VKGASGQALQ NLNIMLGYPE TTGLLHQPLF

P
Length:401
Mass (Da):44,136
Last modified:August 30, 2005 - v2
Checksum:i70A1CFECD1EAD162
GO

Sequence cautioni

The sequence AAC62122 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005169 Genomic DNA. Translation: AAC62122.2. Sequence problems.
CP002685 Genomic DNA. Translation: AEC06945.1.
CP002685 Genomic DNA. Translation: AEC06946.1.
AY058082 mRNA. Translation: AAL24190.1.
AY090309 mRNA. Translation: AAL90970.1.
PIRiA84583.
RefSeqiNP_180668.5. NM_128666.5.
NP_565461.4. NM_127552.7.
NP_849993.4. NM_179662.6.
UniGeneiAt.21379.
At.38263.
At.74911.

Genome annotation databases

EnsemblPlantsiAT2G19940.1; AT2G19940.1; AT2G19940.
AT2G19940.2; AT2G19940.2; AT2G19940.
GeneIDi816513.
817666.
GrameneiAT2G19940.1; AT2G19940.1; AT2G19940.
AT2G19940.2; AT2G19940.2; AT2G19940.
KEGGiath:AT2G19940.
ath:AT2G31100.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005169 Genomic DNA. Translation: AAC62122.2. Sequence problems.
CP002685 Genomic DNA. Translation: AEC06945.1.
CP002685 Genomic DNA. Translation: AEC06946.1.
AY058082 mRNA. Translation: AAL24190.1.
AY090309 mRNA. Translation: AAL90970.1.
PIRiA84583.
RefSeqiNP_180668.5. NM_128666.5.
NP_565461.4. NM_127552.7.
NP_849993.4. NM_179662.6.
UniGeneiAt.21379.
At.38263.
At.74911.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XYGX-ray2.19A/B/C/D43-401[»]
2Q49X-ray2.19A/B/C/D43-401[»]
ProteinModelPortaliQ93Z70.
SMRiQ93Z70. Positions 57-401.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ93Z70. 1 interaction.
STRINGi3702.AT2G19940.1.

Proteomic databases

PaxDbiQ93Z70.
PRIDEiQ93Z70.

Protocols and materials databases

DNASUi816513.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G19940.1; AT2G19940.1; AT2G19940.
AT2G19940.2; AT2G19940.2; AT2G19940.
GeneIDi816513.
817666.
GrameneiAT2G19940.1; AT2G19940.1; AT2G19940.
AT2G19940.2; AT2G19940.2; AT2G19940.
KEGGiath:AT2G19940.
ath:AT2G31100.

Organism-specific databases

TAIRiAT2G19940.

Phylogenomic databases

eggNOGiKOG4354. Eukaryota.
COG0002. LUCA.
InParanoidiQ93Z70.
KOiK00145.
OMAiTFVPHLT.
OrthoDBiEOG09360B0T.
PhylomeDBiQ93Z70.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00108.
BioCyciARA:AT2G19940-MONOMER.
ARA:GQT-2201-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ93Z70.
PROiQ93Z70.

Gene expression databases

ExpressionAtlasiQ93Z70. baseline and differential.
GenevisibleiQ93Z70. AT.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00150. ArgC_type1. 1 hit.
InterProiIPR023013. AGPR_AS.
IPR000706. AGPR_type-1.
IPR016040. NAD(P)-bd_dom.
IPR000534. Semialdehyde_DH_NAD-bd.
IPR012280. Semialdhyde_DH_dimer_dom.
[Graphical view]
PfamiPF01118. Semialdhyde_dh. 1 hit.
PF02774. Semialdhyde_dhC. 1 hit.
[Graphical view]
SMARTiSM00859. Semialdhyde_dh. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01850. argC. 1 hit.
PROSITEiPS01224. ARGC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARGC_ARATH
AccessioniPrimary (citable) accession number: Q93Z70
Secondary accession number(s): O82187
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: August 30, 2005
Last modified: September 7, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.