Reviewed, UniProtKB/Swiss-Prot Q93Z66 (KPRS3_ARATH)

Names and origin

Protein names

Recommended name:
    Ribose-phosphate pyrophosphokinase 3
    EC=2.7.6.1
Alternative name(s):
    Phosphoribosyl pyrophosphate synthetase 3

Gene names

Name:	PRS3
Ordered Locus Names:	At1g10700
ORF Names:	T16B5.16, F20B24.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.
Sequence caution	The sequence AAD31343.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAF17658.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	Ribose-phosphate pyrophosphokinase 3		PRO_0000141094
Regions
	Region	314 – 329	16	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	231	1	Magnesium Potential
	Metal binding	233	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q93Z66-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 606F5C00AE98E007	FASTA	411	44,697
	10 20 30 40 50 60 MAAISPANAT TAASLSLPQF SSTSSSLSSS SSPSFLNFKT ASVSNRCIKC GVRSLENHSG 70 80 90 100 110 120 HRSLDFLSNG DPISLINPNS SSPITMAAAT SESGSKSSKR VCLFHSDETR DLAERIVAKS 130 140 150 160 170 180 DCIELRSINW KKFDDGFPNL FIQNAQGIRG QHVAFLASFS SPAVIFEQLS VIYALPKLFV 190 200 210 220 230 240 SSFTLVLPFF PTGTSERMED EGDVATAFTL ARILSNIPTS RGGPTSLVTF DIHALQERFY 250 260 270 280 290 300 FGDTILPCFE SGIPLLKSRL QSLPDSDNIS IAFPDDGAWK RFHKQLQHYP TIVCNKVRMG 310 320 330 340 350 360 DKRIVRIKEG DAEGRHVVIV DDLVQSGGTL IECQKVLAAH GAAKISAYVT HGIFPRSSWK 370 380 390 400 410 RFKLDTKGDP AEGLSYFWIT DSCGMTVKEV MNKPPFEVLS LAGSIASALQ V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Cloning and sequencing of cDNAs specifying a novel class of phosphoribosyl diphosphate synthase in Arabidopsis thaliana." Krath B.N., Eriksen T.A., Poulsen T.S., Hove-Jensen B. Biochim. Biophys. Acta 1430:403-408(1999) [PubMed: 10082968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 42-411. Strain: cv. Columbia. Tissue: Leaf.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC007354 Genomic DNA. Translation: AAD31343.1. Sequence problems. AC009398 Genomic DNA. Translation: AAF17658.1. Sequence problems. AY058091 mRNA. Translation: AAL24199.1. AY090310 mRNA. Translation: AAL90971.1. AJ012406 mRNA. Translation: CAB43552.1. Different initiation.
IPI	IPI00536204.
PIR	D86240. T52591.
RefSeq	NP_172540.1.
UniGene	At.16545
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q93Z66.
Genome annotation databases
GeneID	837613.
GenomeReviews	Gene locus AT1G10700 in contig CT485782_GR.
KEGG	ath:AT1G10700.
NMPDR	fig|3702.1.peg.1318.
Organism-specific databases
GeneFarm	4835. 468.
TAIR	At1g10700.
Phylogenomic databases
OMA	FESGIPL.
Enzyme and pathway databases
BRENDA	2.7.6.1. 302.
Gene expression databases
Genevestigator	Q93Z66.
GermOnline	AT1G10700. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000842. PRib-PP_synthetase_CS. IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHETASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS3_ARATH

Accession

Primary (citable) accession number: Q93Z66
Secondary accession number(s): Q9SAD2, Q9SGY0, Q9XFR3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	December 1, 2001
Last modified:	November 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)