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Q93Z53

- PKP3_ARATH

UniProt

Q93Z53 - PKP3_ARATH

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Protein

Plastidial pyruvate kinase 3, chloroplastic

Gene

PKP3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for plastidial pyruvate kinase activity.By similarity

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+1 Publication
  • K(+)1 Publication

pH dependencei

Optimum pH is 7.8-8.0.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei129 – 1291SubstrateBy similarity
Metal bindingi131 – 1311PotassiumBy similarity
Metal bindingi164 – 1641PotassiumBy similarity
Metal bindingi165 – 1651Potassium; via carbonyl oxygenBy similarity
Sitei314 – 3141Transition state stabilizerBy similarity
Metal bindingi316 – 3161MagnesiumSequence Analysis
Binding sitei339 – 3391Substrate; via amide nitrogenBy similarity
Metal bindingi340 – 3401MagnesiumBy similarity
Binding sitei340 – 3401Substrate; via amide nitrogenBy similarity
Binding sitei372 – 3721SubstrateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: UniProtKB
  3. potassium ion binding Source: UniProtKB
  4. pyruvate kinase activity Source: TAIR

GO - Biological processi

  1. fatty acid biosynthetic process Source: TAIR
  2. glycolytic process Source: UniProtKB-UniPathway
  3. seed maturation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

ReactomeiREACT_239741. Regulation of gene expression in beta cells.
REACT_248260. Glycolysis.
REACT_260729. ChREBP activates metabolic gene expression.
SABIO-RKQ93Z53.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Plastidial pyruvate kinase 3, chloroplastic (EC:2.7.1.40)
Short name:
PKp3
Alternative name(s):
Pyruvate kinase I
Pyruvate kinase isozyme B2, chloroplastic
Short name:
PKP-BETA2
Short name:
Plastidic pyruvate kinase beta subunit 2
Gene namesi
Name:PKP3
Ordered Locus Names:At1g32440
ORF Names:F5D14.22
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G32440.

Subcellular locationi

Plastidchloroplast stroma 2 Publications

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5555ChloroplastSequence AnalysisAdd
BLAST
Chaini56 – 571516Plastidial pyruvate kinase 3, chloroplasticPRO_0000416989Add
BLAST

Proteomic databases

PaxDbiQ93Z53.
PRIDEiQ93Z53.

Expressioni

Tissue specificityi

Expressed at low levels in roots, leaves, inflorescences, siliques, pollen, seeds and flowers.2 Publications

Gene expression databases

GenevestigatoriQ93Z53.

Interactioni

Subunit structurei

Oligomer of alpha and beta subunits.1 Publication

Protein-protein interaction databases

STRINGi3702.AT1G32440.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ93Z53.
SMRiQ93Z53. Positions 99-512.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0469.
HOGENOMiHOG000021559.
InParanoidiQ93Z53.
KOiK00873.
OMAiQRPEDIH.
PhylomeDBiQ93Z53.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q93Z53-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAYGQISSG MTVDPQVLSS SRNIGVSLSP LRRTLIGAGV RSTSISLRQC
60 70 80 90 100
SLSVRSIKIS EDSRKPKAYA ENGAFDVGVL DSSSYRLADS RTSSNDSRRK
110 120 130 140 150
TKIVCTIGPS SSSREMIWKL AEAGMNVARL NMSHGDHASH QITIDLVKEY
160 170 180 190 200
NSLFVDKAIA IMLDTKGPEV RSGDVPQPIF LEEGQEFNFT IKRGVSLKDT
210 220 230 240 250
VSVNYDDFVN DVEVGDILLV DGGMMSLAVK SKTSDLVKCV VIDGGELQSR
260 270 280 290 300
RHLNVRGKSA TLPSITDKDW EDIKFGVDNQ VDFYAVSFVK DAKVVHELKN
310 320 330 340 350
YLKTCSADIS VIVKIESADS IKNLPSIISA CDGAMVARGD LGAELPIEEV
360 370 380 390 400
PLLQEEIIRR CRSIHKPVIV ATNMLESMIN HPTPTRAEVS DIAIAVREGA
410 420 430 440 450
DAIMLSGETA HGKFPLKAVN VMHTVALRTE ASLPVRTSAS RTTAYKGHMG
460 470 480 490 500
QMFAFHASIM ANTLSSPLIV FTRTGSMAVL LSHYRPSATI FAFTNQRRIM
510 520 530 540 550
QRLALYQGVM PIYMEFSDDA EDTYARSLKL LQDENMLKEG QHVTLVQSGS
560 570
QPIWREESTH LIQVRKIKIG G
Length:571
Mass (Da):62,615
Last modified:December 1, 2001 - v1
Checksum:i4C61979F639274B1
GO

Sequence cautioni

The sequence AAF81342.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007767 Genomic DNA. Translation: AAF81342.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE31487.1.
AY058121 mRNA. Translation: AAL25538.1.
BT001147 mRNA. Translation: AAN64538.1.
PIRiF86449.
RefSeqiNP_564402.1. NM_102979.1.
UniGeneiAt.66798.

Genome annotation databases

EnsemblPlantsiAT1G32440.1; AT1G32440.1; AT1G32440.
GeneIDi840138.
KEGGiath:AT1G32440.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007767 Genomic DNA. Translation: AAF81342.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE31487.1 .
AY058121 mRNA. Translation: AAL25538.1 .
BT001147 mRNA. Translation: AAN64538.1 .
PIRi F86449.
RefSeqi NP_564402.1. NM_102979.1.
UniGenei At.66798.

3D structure databases

ProteinModelPortali Q93Z53.
SMRi Q93Z53. Positions 99-512.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT1G32440.1-P.

Proteomic databases

PaxDbi Q93Z53.
PRIDEi Q93Z53.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G32440.1 ; AT1G32440.1 ; AT1G32440 .
GeneIDi 840138.
KEGGi ath:AT1G32440.

Organism-specific databases

TAIRi AT1G32440.

Phylogenomic databases

eggNOGi COG0469.
HOGENOMi HOG000021559.
InParanoidi Q93Z53.
KOi K00873.
OMAi QRPEDIH.
PhylomeDBi Q93Z53.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .
Reactomei REACT_239741. Regulation of gene expression in beta cells.
REACT_248260. Glycolysis.
REACT_260729. ChREBP activates metabolic gene expression.
SABIO-RK Q93Z53.

Gene expression databases

Genevestigatori Q93Z53.

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "A heteromeric plastidic pyruvate kinase complex involved in seed oil biosynthesis in Arabidopsis."
    Andre C., Froehlich J.E., Moll M.R., Benning C.
    Plant Cell 19:2006-2022(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, SUBUNIT, SUBCELLULAR LOCATION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, GENE FAMILY.
  5. "Function of plastidial pyruvate kinases in seeds of Arabidopsis thaliana."
    Baud S., Wuilleme S., Dubreucq B., de Almeida A., Vuagnat C., Lepiniec L., Miquel M., Rochat C.
    Plant J. 52:405-419(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
    Strain: cv. Wassilewskija.
  6. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
    Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
    PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPKP3_ARATH
AccessioniPrimary (citable) accession number: Q93Z53
Secondary accession number(s): Q9LQL3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: December 1, 2001
Last modified: November 26, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3