Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Plastidial pyruvate kinase 3, chloroplastic

Gene

PKP3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for plastidial pyruvate kinase activity.By similarity

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+1 Publication
  • K+1 Publication

pH dependencei

Optimum pH is 7.8-8.0.1 Publication

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase GAPC2, cytosolic (GAPC2), Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic (GAPC1)
  2. Phosphoglycerate kinase 3, cytosolic (PGK3)
  3. Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2 (At3g08590), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 (PGM1)
  4. Enolase 1, chloroplastic (ENO1), Cytosolic enolase 3 (ENO3), Bifunctional enolase 2/transcriptional activator (ENO2)
  5. Pyruvate kinase (AXX17_At3g24740), Pyruvate kinase (AXX17_At5g51800), Pyruvate kinase (AXX17_At5g63250), Pyruvate kinase (AXX17_At3g50280), Pyruvate kinase (AXX17_ATUG04870), Pyruvate kinase (MAH20.13), Pyruvate kinase (MCD7.8), Pyruvate kinase (MBK5.16), Pyruvate kinase (F1I16_60), Pyruvate kinase (At3g25960), Pyruvate kinase (AXX17_At3g50440), Plastidial pyruvate kinase 3, chloroplastic (PKP3), Pyruvate kinase (AXX17_At5g08490), Pyruvate kinase (At3g52990), Pyruvate kinase (AXX17_At5g55530), Pyruvate kinase (AXX17_At3g28070), Pyruvate kinase (At2g36580), Pyruvate kinase (AXX17_At3g47400), Pyruvate kinase, Probable pyruvate kinase, cytosolic isozyme (At4g26390), Pyruvate kinase (F8J2_160), Pyruvate kinase (At3g52990), Pyruvate kinase (AXX17_At3g03460), Plastidial pyruvate kinase 2 (PKP2), Pyruvate kinase (AXX17_At2g33300), Pyruvate kinase (At3g04050), Pyruvate kinase (AXX17_At4g30430), Pyruvate kinase (F1I16_220), Plastidial pyruvate kinase 1, chloroplastic (PKP1), Pyruvate kinase (AXX17_At1g33230), Pyruvate kinase (At5g63680), Pyruvate kinase, Plastidial pyruvate kinase 4, chloroplastic (PKP4)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei129SubstrateBy similarity1
Metal bindingi131PotassiumBy similarity1
Metal bindingi133PotassiumBy similarity1
Metal bindingi164PotassiumBy similarity1
Metal bindingi165Potassium; via carbonyl oxygenBy similarity1
Binding sitei314Substrate; via amide nitrogenBy similarity1
Sitei314Transition state stabilizerBy similarity1
Metal bindingi316MagnesiumBy similarity1
Binding sitei339Substrate; via amide nitrogenBy similarity1
Metal bindingi340MagnesiumBy similarity1
Binding sitei340Substrate; via amide nitrogenBy similarity1
Binding sitei372SubstrateBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: UniProtKB-KW
  • magnesium ion binding Source: UniProtKB
  • potassium ion binding Source: UniProtKB
  • pyruvate kinase activity Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

SABIO-RKiQ93Z53
UniPathwayiUPA00109; UER00188

Names & Taxonomyi

Protein namesi
Recommended name:
Plastidial pyruvate kinase 3, chloroplastic (EC:2.7.1.40)
Short name:
PKp3
Alternative name(s):
Pyruvate kinase I
Pyruvate kinase isozyme B2, chloroplastic
Short name:
PKP-BETA2
Short name:
Plastidic pyruvate kinase beta subunit 2
Gene namesi
Name:PKP3
Ordered Locus Names:At1g32440
ORF Names:F5D14.22
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G32440
TAIRilocus:2033760 AT1G32440

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 55ChloroplastSequence analysisAdd BLAST55
ChainiPRO_000041698956 – 571Plastidial pyruvate kinase 3, chloroplasticAdd BLAST516

Proteomic databases

PaxDbiQ93Z53
PRIDEiQ93Z53

Expressioni

Tissue specificityi

Expressed at low levels in roots, leaves, inflorescences, siliques, pollen, seeds and flowers.2 Publications

Gene expression databases

ExpressionAtlasiQ93Z53 baseline and differential
GenevisibleiQ93Z53 AT

Interactioni

Subunit structurei

Oligomer of alpha and beta subunits.1 Publication

Protein-protein interaction databases

STRINGi3702.AT1G32440.1

Structurei

3D structure databases

ProteinModelPortaliQ93Z53
SMRiQ93Z53
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2323 Eukaryota
COG0469 LUCA
HOGENOMiHOG000021559
InParanoidiQ93Z53
KOiK00873
OMAiVTRCERT
OrthoDBiEOG09360578
PhylomeDBiQ93Z53

Family and domain databases

Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 1 hit
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR015795 Pyrv_Knase_C
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PF02887 PK_C, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q93Z53-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAYGQISSG MTVDPQVLSS SRNIGVSLSP LRRTLIGAGV RSTSISLRQC
60 70 80 90 100
SLSVRSIKIS EDSRKPKAYA ENGAFDVGVL DSSSYRLADS RTSSNDSRRK
110 120 130 140 150
TKIVCTIGPS SSSREMIWKL AEAGMNVARL NMSHGDHASH QITIDLVKEY
160 170 180 190 200
NSLFVDKAIA IMLDTKGPEV RSGDVPQPIF LEEGQEFNFT IKRGVSLKDT
210 220 230 240 250
VSVNYDDFVN DVEVGDILLV DGGMMSLAVK SKTSDLVKCV VIDGGELQSR
260 270 280 290 300
RHLNVRGKSA TLPSITDKDW EDIKFGVDNQ VDFYAVSFVK DAKVVHELKN
310 320 330 340 350
YLKTCSADIS VIVKIESADS IKNLPSIISA CDGAMVARGD LGAELPIEEV
360 370 380 390 400
PLLQEEIIRR CRSIHKPVIV ATNMLESMIN HPTPTRAEVS DIAIAVREGA
410 420 430 440 450
DAIMLSGETA HGKFPLKAVN VMHTVALRTE ASLPVRTSAS RTTAYKGHMG
460 470 480 490 500
QMFAFHASIM ANTLSSPLIV FTRTGSMAVL LSHYRPSATI FAFTNQRRIM
510 520 530 540 550
QRLALYQGVM PIYMEFSDDA EDTYARSLKL LQDENMLKEG QHVTLVQSGS
560 570
QPIWREESTH LIQVRKIKIG G
Length:571
Mass (Da):62,615
Last modified:December 1, 2001 - v1
Checksum:i4C61979F639274B1
GO

Sequence cautioni

The sequence AAF81342 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007767 Genomic DNA Translation: AAF81342.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE31487.1
AY058121 mRNA Translation: AAL25538.1
BT001147 mRNA Translation: AAN64538.1
PIRiF86449
RefSeqiNP_564402.1, NM_102979.2
UniGeneiAt.66798

Genome annotation databases

EnsemblPlantsiAT1G32440.1; AT1G32440.1; AT1G32440
GeneIDi840138
GrameneiAT1G32440.1; AT1G32440.1; AT1G32440
KEGGiath:AT1G32440

Similar proteinsi

Entry informationi

Entry nameiPKP3_ARATH
AccessioniPrimary (citable) accession number: Q93Z53
Secondary accession number(s): Q9LQL3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: December 1, 2001
Last modified: April 25, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health