Reviewed, UniProtKB/Swiss-Prot Q93Z25 (PEL22_ARATH)

Names and origin

Protein names

Recommended name:
    Probable pectate lyase 22
    EC=4.2.2.2

Gene names

Ordered Locus Names:	At5g63180
ORF Names:	MDC12_15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion. Required for its activity By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Sequence similarities	Belongs to the polysaccharide lyase 1 family.

Ontologies

Keywords
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 45	45	Potential
	Chain	46 – 432	387	Probable pectate lyase 22		PRO_0000024887
Sites
	Active site	308	1	Potential
	Metal binding	228	1	Calcium By similarity
	Metal binding	252	1	Calcium By similarity
	Metal binding	256	1	Calcium By similarity
Amino acid modifications
	Glycosylation	65	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q93Z25-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 870772E83104C1A2	FASTA	432	47,895
	10 20 30 40 50 60 MFRPNSLLIP SNLSTTKSQR NTMLNSSYLS FALIFFCCIL FSALASSLPV SDPELVVEEV 70 80 90 100 110 120 HRKINESISR RKLGFFSCGS GNPIDDCWRC DKDWEKNRKR LADCGIGFGK NAIGGRDGEI 130 140 150 160 170 180 YVVTDPGNDD PVNPRPGTLR YAVIQDEPLW IIFKRDMTIQ LKEELIMNSF KTLDGRGASV 190 200 210 220 230 240 HISGGPCITI QYVTNIIIHG LHIHDCKQGG NTYVRDSPEH YGYRTVSDGD GVSIFGGSHV 250 260 270 280 290 300 WVDHCSLSNC NDGLIDAIRG STAITISNNY LTHHNKVMLL GHSDTYEQDK NMQVTIAFNH 310 320 330 340 350 360 FGEGLVQRMP RCRHGYFHVV NNDYTHWEMY AIGGSANPTI NSQGNRFLAP DDSSSKEVTK 370 380 390 400 410 420 HEDAPEDEWR NWNWRSEGDL LLNGAFFTYS GAGPAKSSSY SKASSLAARP SSHVGEITIA 430 SGALSCKRGS HC

References

[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones." Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S. DNA Res. 4:401-414(1997) [PubMed: 9501997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB008265 Genomic DNA. Translation: BAB10560.1. Different initiation. AY058197 mRNA. Translation: AAL25610.1.
IPI	IPI00519121.
RefSeq	NP_568967.1.
UniGene	At.8839
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Proteomic databases
PRIDE	Q93Z25.
Genome annotation databases
GeneID	836439.
GenomeReviews	Gene locus AT5G63180 in contig BA000015_GR.
KEGG	ath:AT5G63180.
NMPDR	fig|3702.1.peg.28366.
Organism-specific databases
TAIR	At5g63180.
Enzyme and pathway databases
BRENDA	4.2.2.2. 302.
Gene expression databases
ArrayExpress	Q93Z25.
Genevestigator	Q93Z25.
GermOnline	AT5G63180. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR018082. AmbAllergen. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
PRINTS	PR00807. AMBALLERGEN.
SMART	SM00656. Amb_all. 1 hit. SM00710. PbH1. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PEL22_ARATH

Accession

Primary (citable) accession number: Q93Z25
Secondary accession number(s): Q9FMK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	December 1, 2001
Last modified:	October 13, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)