Reviewed, UniProtKB/Swiss-Prot Q93Z08 (E136_ARATH)

Names and origin

Protein names

Recommended name:
    Glucan endo-1,3-beta-glucosidase 6
    EC=3.2.1.39
Alternative name(s):
    (1->3)-beta-glucan endohydrolase 6
      Short name=(1->3)-beta-glucanase 6
    Beta-1,3-endoglucanase 6
      Short name=Beta-1,3-glucanase 6

Gene names

Ordered Locus Names:	At5g58090
ORF Names:	K21L19.12, K21L19_70

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor Ref.3 Ref.4.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Cell membrane Membrane
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	anchored to plasma membrane Ref.3 Inferred from direct assay. Source: TAIR
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 455	434	Glucan endo-1,3-beta-glucosidase 6		PRO_0000011892
	Propeptide	456 – 477	22	Removed in mature form Potential		PRO_0000011893
Sites
	Active site	262	1	Nucleophile By similarity
	Active site	323	1	Proton donor By similarity
Amino acid modifications
	Lipidation	455	1	GPI-anchor amidated glycine Potential
	Glycosylation	97	1	N-linked (GlcNAc...) Potential
	Glycosylation	124	1	N-linked (GlcNAc...) Potential
	Glycosylation	406	1	N-linked (GlcNAc...) Potential
	Glycosylation	429	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	219	1	F → L in AAL24251. Ref.2
	Sequence conflict	219	1	F → L in AAN18179. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q93Z08-1 [UniParc]. Last modified October 31, 2003. Version 2. Checksum: B8FE6F29218DF57C	FASTA	477	52,211
	10 20 30 40 50 60 MGWGSVLLLL AVALLCQRAS SIGANWGTQA SHPLPPDIVV RMLRENGIQK VKLFDAEYDT 70 80 90 100 110 120 LRALGKSGIE VMVGIPNEML ATLASSLKAA EKWVAKNVST HISTDNVNIR YVAVGNEPFL 130 140 150 160 170 180 STYNGSYLST TFPALRNIQI AIIKAGLQNQ VKVTCPLNAD VYDSSTTFPS GGDFRANIRD 190 200 210 220 230 240 LMITIVKFLS ENGGPFTVNI YPYISLYTNP DFPVDYAFFD GNAQPLNDGG TFYYNMFDAN 250 260 270 280 290 300 YDTLVHALEK NGFGNMPIII GEIGWPTDGD SNANLDYAKK FNQGFMAHIS GGKGTPRRPG 310 320 330 340 350 360 PIDAYLFSLI DEDAKSVQPG YFERHWGIFT FDGLPKYALN LGTTNTGALI QAKGVRYLER 370 380 390 400 410 420 KWCVMKPNVR LDDPQVAPAV SYACSLGDCT SLGVGTSCAN LDGKQNISYA FNSYYQIQDQ 430 440 450 460 470 LDTACKFPNI SEVTKTDPST GTCRFPIMIE PYYGGAAREH GFFFPLLMVA AIAVSIF

References

[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed: 10718197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Identification of glycosylphosphatidylinositol-anchored proteins in Arabidopsis. A proteomic and genomic analysis." Borner G.H.H., Lilley K.S., Stevens T.J., Dupree P. Plant Physiol. 132:568-577(2003) [PubMed: 12805588] [Abstract] Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Tissue: Callus.
[4]	"Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins." Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N. Mol. Cell. Proteomics 2:1261-1270(2003) [PubMed: 14517339] [Abstract] Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB024029 Genomic DNA. Translation: BAB11001.1. AY058864 mRNA. Translation: AAL24251.1. BT000612 mRNA. Translation: AAN18179.1.
IPI	IPI00518292.
RefSeq	NP_200617.2.
UniGene	At.9709
3D structure databases
SMR	Q93Z08. Positions 22-341, 355-447.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q93Z08. 1 interaction.
Protein family/group databases
CAZy	CBM43. Carbohydrate-Binding Module Family 43. GH17. Glycoside Hydrolase Family 17.
Proteomic databases
PRIDE	Q93Z08.
Genome annotation databases
GeneID	835921.
GenomeReviews	Gene locus AT5G58090 in contig BA000015_GR.
KEGG	ath:AT5G58090.
NMPDR	fig|3702.1.peg.27776.
Organism-specific databases
TAIR	At5g58090.
Phylogenomic databases
HOGENOM	HBG605194.
InParanoid	Q93Z08.
OMA	DLMLTIV.
Enzyme and pathway databases
BRENDA	3.2.1.39. 302.
Gene expression databases
ArrayExpress	Q93Z08.
Genevestigator	Q93Z08.
GermOnline	AT5G58090. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000490. Glyco_hydro_17. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. IPR012946. X8. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00332. Glyco_hydro_17. 1 hit. PF07983. X8. 1 hit. [Graphical view]
SMART	SM00768. X8. 1 hit. [Graphical view]
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E136_ARATH

Accession

Primary (citable) accession number: Q93Z08
Secondary accession number(s): Q9FGT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	October 31, 2003
Last modified:	February 9, 2010
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)