Q93YS2 (P2C51_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable protein phosphatase 2C 51
Short name=AtPP2C51
EC=3.1.3.16

Gene names

Ordered Locus Names:	At3g63340
ORF Names:	F16M2.190, MAA21.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	528 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.
Sequence caution	The sequence AEE80468.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene has been split into 2 genes: At3g63330 and At3g63340. The sequence AEE80469.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene has been split into 2 genes: At3g63330 and At3g63340. The sequence CAB86435.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene has been split into 2 genes: At3g63330 and At3g63340.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q93YS2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 528	528	Probable protein phosphatase 2C 51		PRO_0000367975
Regions
	Transmembrane	8 – 28	21	Helical; Potential
	Domain	70 – 438	369	PP2C-like
Sites
	Metal binding	117	1	Manganese 1 By similarity
	Metal binding	117	1	Manganese 2 By similarity
	Metal binding	118	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	385	1	Manganese 2 By similarity
	Metal binding	436	1	Manganese 2 By similarity
Experimental info
	Sequence conflict	199	1	D → G in AAL24137. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 14, 2009. Version 2. Checksum: 803DF76D1D45ADE5	FASTA	528	58,354
	10 20 30 40 50 60 MTSSIKSSLL NLGLLIIFFV FFFLVINCRG ESSTCLAVYK QGGAPAVFQS PKCPRWILQN 70 80 90 100 110 120 WGSPTHSGAG RCHTAAIQGR RNYQEDRLLC ALDLRIPFPG KTGTPKDVLV GIAAVFDGHN 130 140 150 160 170 180 GAEASDMASK LLLDYFALHI NFLLDATFSA MTRKLIGRFP TKGDHSVILH GVSRDEIMHL 190 200 210 220 230 240 YNLDFQMQFR DSLPLHFDDS LPLDIMKEAL LRAIHDIDVT FTKEASNRKL NSGSTATIAL 250 260 270 280 290 300 IADGQLMVAS IGDSKALLCS EKFETLEEAR ATLVKLYRER RRNRGSSPSR FSDFKLEHGN 310 320 330 340 350 360 GLLRFIAKEL TKDHHPNRED EKIRVEAAGG YVTEWAGVPR VNGQLTVSRA IGDLTYRSYG 370 380 390 400 410 420 VISAPEVMDW QPLVANDSFL VVSSDGIFEK LEVQEVCDLL WEVNNQTSSG AGVPSYCSIS 430 440 450 460 470 480 LADCLVNTAF EKGSMDNMAA VVVPLKSNLV TQLQRKEQSM NDNKDKIASA LPCSNCTLPL 490 500 510 520 PNDINLGPLQ LKQAQPLGTM FNRLLRLKTE VFAAFICQRT LLGHLKGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL138648 Genomic DNA. Translation: CAB86435.1. Sequence problems. CP002686 Genomic DNA. Translation: AEE80468.1. Sequence problems. CP002686 Genomic DNA. Translation: AEE80469.1. Sequence problems. AY059789 mRNA. Translation: AAL24137.1.
IPI	IPI00542078. IPI00992809.
PIR	T48123.
UniGene	At.26629.
3D structure databases
ProteinModelPortal	Q93YS2.
SMR	Q93YS2. Positions 199-445.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT3G63340.1-P.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
TAIR	At3g63340.
Phylogenomic databases
eggNOG	COG0631.
InParanoid	Q93YS2.
PhylomeDB	Q93YS2.
ProtClustDB	CLSN2689350.
Gene expression databases
ArrayExpress	Q93YS2.
Genevestigator	Q93YS2.
Family and domain databases
Gene3D	3.60.40.10. 3 hits.
InterPro	IPR001932. PP2C-like. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C51_ARATH

Accession

Primary (citable) accession number: Q93YS2
Secondary accession number(s): F4J0Z1, F4J0Z2, Q9M1V6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 14, 2009
Last modified:	May 1, 2013
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program