Reviewed, UniProtKB/Swiss-Prot Q93Y52 (IPYR1_CHLRE)

Names and origin

Protein names

Recommended name:
    Soluble inorganic pyrophosphatase 1, chloroplastic
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate phospho-hydrolase 1
      Short name=PPase 1

Gene names

Name:	ppa1
Synonyms:	ppaI

Organism

Chlamydomonas reinhardtii

Taxonomic identifier

3055 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Chlorophyta › Chlorophyceae › Chlamydomonadales › Chlamydomonadaceae › Chlamydomonas

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate. Ref.1
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity. UniProtKB P00817
Subunit structure	Monomer. Ref.1
Subcellular location	Plastid › chloroplast. Ref.1
Post-translational modification	The N-terminus is blocked. Ref.1
Sequence similarities	Belongs to the PPase family.
Biophysicochemical properties	Kinetic parameters: KM=10.5 µM for Mg2-PPi Ref.1 pH dependence: Optimum pH is 7.5. Ref.1
Mass spectrometry	Molecular mass is 29850 Da from positions ? - 280. Determined by MALDI. Ref.1

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	inorganic diphosphatase activity Ref.1 Inferred from direct assay. Source: UniProtKB magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast
	Chain	? – 280		Soluble inorganic pyrophosphatase 1, chloroplastic		PRO_0000253938
Sites
	Metal binding	152	1	Magnesium 1 By similarity UniProtKB P00817
	Metal binding	157	1	Magnesium 1 By similarity UniProtKB P00817
	Metal binding	157	1	Magnesium 2 By similarity UniProtKB P00817
	Metal binding	189	1	Magnesium 1 By similarity UniProtKB P00817
	Binding site	120	1	Inorganic pyrophosphate By similarity UniProtKB P00817

Sequences

	Sequence		Length	Mass (Da)
	Q93Y52-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 5D952A188644BC43	FASTA	280	31,134
	10 20 30 40 50 60 MALAIRSSLR AAAMGRKAFR QAVPVRVAPA QRVRSVTTAS AEITAYSVEE KGPKDSLEYR 70 80 90 100 110 120 MFFKQGAKEV SCWHEIPLYA GDGHLHYICE IPKETSAKME VATDEPRTPI KQDVKKGKLR 130 140 150 160 170 180 FYPYNINWNY GMLPQTWEDP GHTDATLGAA GDNDPVDVVE IGAAAAKRGG VYKVKPVGVL 190 200 210 220 230 240 AMIDDGELDW KVIAISADDP KAALCNDVED VEKHFPGEIQ KVLEWFRDYK IPDGKPANKF 250 260 270 280 GYDNKCMNKE FTLNVIKETH EAYVKLKSGA RANSEELSLI

References

[1]

"A novel subfamily of monomeric inorganic pyrophosphatases in photosynthetic eukaryotes." Gomez-Garcia M.R., Losada M., Serrano A. Biochem. J. 395:211-221(2006) [PubMed: 16313235] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, MASS SPECTROMETRY. Strain: 21gr / CC-1690.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ298231 mRNA. Translation: CAC42762.1.
RefSeq	XP_001702577.1.
UniGene	Cre.4641
3D structure databases
HSSP	HSSP built from PDB template 1WGI based on UniProtKB P00817.
ModBase	Search...
Proteomic databases
PRIDE	Q93Y52.
Genome annotation databases
GeneID	5728217.
KEGG	cre:CHLREDRAFT_133620.
Enzyme and pathway databases
BRENDA	3.6.1.1. 144.
Family and domain databases
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
ProDom	PD002014. Inorg_pphsph. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR1_CHLRE

Accession

Primary (citable) accession number: Q93Y52

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	December 1, 2001
Last modified:	June 16, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)