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Q93Y37

- MNS3_ARATH

UniProt

Q93Y37 - MNS3_ARATH

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Protein
Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3
Gene
MNS3, At1g30000, T1P2.10
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Class I alpha-mannosidase essential for early N-glycan processing. Removes preferentially alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man8GlcNAc2. Involved in root development and cell wall biosynthesis.1 Publication

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

Cofactori

Calcium or manganese. Magnesium can be used to a lesser extent.

Enzyme regulationi

Inhibited by kifunensine and 1-deoxymannojirimycin, but not by swainsonine.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei212 – 2121Proton donor By similarity
Active sitei357 – 3571 By similarity
Active sitei526 – 5261 By similarity

GO - Molecular functioni

  1. alpha-mannosidase activity Source: TAIR
  2. calcium ion binding Source: InterPro
  3. mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. N-glycan processing Source: TAIR
  2. root development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calcium, Magnesium, Manganese

Enzyme and pathway databases

BioCyciARA:AT1G30000-MONOMER.
ReactomeiREACT_190970. ER Quality Control Compartment (ERQC).
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 (EC:3.2.1.113)
Gene namesi
Name:MNS3
Ordered Locus Names:At1g30000
ORF Names:T1P2.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G30000.

Subcellular locationi

Golgi apparatuscis-Golgi network membrane Inferred; Single-pass type II membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4343Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei44 – 6421Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini65 – 624560Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: TAIR
  2. endosome Source: TAIR
  3. integral component of membrane Source: UniProtKB-KW
  4. trans-Golgi network Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Formation of aberrant N-glycan structures.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 624624Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3
PRO_0000397935Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi69 – 691N-linked (GlcNAc...) Reviewed prediction
Glycosylationi114 – 1141N-linked (GlcNAc...) Reviewed prediction
Glycosylationi236 – 2361N-linked (GlcNAc...) Reviewed prediction
Glycosylationi377 – 3771N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi428 ↔ 471 By similarity
Glycosylationi503 – 5031N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ93Y37.
PRIDEiQ93Y37.

Expressioni

Tissue specificityi

Expressed in flowers, siliques, stems, leaves, roots, stamens and sepals.1 Publication

Gene expression databases

GenevestigatoriQ93Y37.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G30000.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ93Y37.
SMRiQ93Y37. Positions 128-620.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG300315.
HOGENOMiHOG000181987.
InParanoidiQ93Y37.
KOiK01230.
OMAiVHFNLHA.
PhylomeDBiQ93Y37.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERiPTHR11742. PTHR11742. 1 hit.
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.

Sequencei

Sequence statusi: Complete.

Q93Y37-1 [UniParc]FASTAAdd to Basket

« Hide

MSKSLPYSVK DIHYDNAKFR HRSPLKVFSQ SLLTLSTKRN YASCSTGKFL    50
ILILFFGVAC LMLMSKSPNE SGLNEKGKVT FVGGLRLGGL LRKPPRLPPR 100
LSPDEGQLRG SSTNGSTISN SDPKWAARQQ SVKEAFDHAW SGYRKYAMGY 150
DELMPISQKG VDGLGGLGAT VVDALDTAMI MGLDNIVSEA GSWVETHLLE 200
RISQKGQVNL FETTIRVLGG LLSAYHLSGG EQGTVNMTHV GPKPVIYLNI 250
AKDLADRLLS AFTSSPTPVP FCDVILHEST AHPAPGGASS TAEVASVQLE 300
FNYLSSISGD PKYSTEAMKV LAHIKTLPKT EGLVPIYISP QTGDFVGENI 350
RLGSRGDSYY EYLIKVWLQQ GAKLNSNFTY LHDMYIEAMK GVRHLLVQNS 400
IPKGLVFVGE LPYGSKGEFS PKMDHLVCFL PGTLALGATK GLTKEQALKE 450
NLLSFEDLEN LKLAEDLAKT CFEMYEVTAT GLAPEIAYFH TKDYTEDGLD 500
GGNKSSMYAN DIIIKPADRH NLLRPETVES LFVLYRITKD TKYRDQGWQI 550
FEAFEKYTKV KSGGYTSLDD VTEVPPHRRD KMETFFLGET LKYLYLLFGD 600
DSVIPLDKFV FNTEAHPLPI RRNT 624
Length:624
Mass (Da):69,069
Last modified:December 1, 2001 - v1
Checksum:iA28B82AD7FAAD29D
GO

Sequence cautioni

The sequence AAG52061.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC022455 Genomic DNA. Translation: AAG52061.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE31166.1.
AY054482 mRNA. Translation: AAK96673.1.
AY093280 mRNA. Translation: AAM13279.1.
PIRiH86423.
RefSeqiNP_564345.1. NM_102740.2.
UniGeneiAt.26476.

Genome annotation databases

EnsemblPlantsiAT1G30000.1; AT1G30000.1; AT1G30000.
GeneIDi839879.
KEGGiath:AT1G30000.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC022455 Genomic DNA. Translation: AAG52061.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE31166.1 .
AY054482 mRNA. Translation: AAK96673.1 .
AY093280 mRNA. Translation: AAM13279.1 .
PIRi H86423.
RefSeqi NP_564345.1. NM_102740.2.
UniGenei At.26476.

3D structure databases

ProteinModelPortali Q93Y37.
SMRi Q93Y37. Positions 128-620.
ModBasei Search...

Protein-protein interaction databases

STRINGi 3702.AT1G30000.1-P.

Protein family/group databases

CAZyi GH47. Glycoside Hydrolase Family 47.

Proteomic databases

PaxDbi Q93Y37.
PRIDEi Q93Y37.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G30000.1 ; AT1G30000.1 ; AT1G30000 .
GeneIDi 839879.
KEGGi ath:AT1G30000.

Organism-specific databases

TAIRi AT1G30000.

Phylogenomic databases

eggNOGi NOG300315.
HOGENOMi HOG000181987.
InParanoidi Q93Y37.
KOi K01230.
OMAi VHFNLHA.
PhylomeDBi Q93Y37.

Enzyme and pathway databases

UniPathwayi UPA00378 .
BioCyci ARA:AT1G30000-MONOMER.
Reactomei REACT_190970. ER Quality Control Compartment (ERQC).

Miscellaneous databases

PROi Q93Y37.

Gene expression databases

Genevestigatori Q93Y37.

Family and domain databases

Gene3Di 1.50.10.50. 1 hit.
InterProi IPR001382. Glyco_hydro_47.
[Graphical view ]
PANTHERi PTHR11742. PTHR11742. 1 hit.
Pfami PF01532. Glyco_hydro_47. 1 hit.
[Graphical view ]
PRINTSi PR00747. GLYHDRLASE47.
SUPFAMi SSF48225. SSF48225. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Class I alpha-mannosidases are required for N-glycan processing and root development in Arabidopsis thaliana."
    Liebminger E., Huttner S., Vavra U., Fischl R., Schoberer J., Grass J., Blaukopf C., Seifert G.J., Altmann F., Mach L., Strasser R.
    Plant Cell 21:3850-3867(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, ENZYME REGULATION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiMNS3_ARATH
AccessioniPrimary (citable) accession number: Q93Y37
Secondary accession number(s): Q9C8R9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: December 1, 2001
Last modified: September 3, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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