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Q93Y37

- MNS3_ARATH

UniProt

Q93Y37 - MNS3_ARATH

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Protein

Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3

Gene

MNS3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Class I alpha-mannosidase essential for early N-glycan processing. Removes preferentially alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man8GlcNAc2. Involved in root development and cell wall biosynthesis.1 Publication

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

Cofactori

Ca2+, Mn2+, Mg2+Note: Ca(2+) or Mn(2+). Mg(2+) can be used to a lesser extent.

Enzyme regulationi

Inhibited by kifunensine and 1-deoxymannojirimycin, but not by swainsonine.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei212 – 2121Proton donorBy similarity
Active sitei357 – 3571By similarity
Active sitei526 – 5261By similarity

GO - Molecular functioni

  1. alpha-mannosidase activity Source: TAIR
  2. calcium ion binding Source: InterPro
  3. mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. N-glycan processing Source: TAIR
  2. root development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calcium, Magnesium, Manganese

Enzyme and pathway databases

BioCyciARA:AT1G30000-MONOMER.
ReactomeiREACT_190970. ER Quality Control Compartment (ERQC).
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 (EC:3.2.1.113)
Gene namesi
Name:MNS3
Ordered Locus Names:At1g30000
ORF Names:T1P2.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G30000.

Subcellular locationi

Golgi apparatuscis-Golgi network membrane 1 Publication; Single-pass type II membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4343CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei44 – 6421Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini65 – 624560LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endosome Source: TAIR
  2. Golgi apparatus Source: TAIR
  3. integral component of membrane Source: UniProtKB-KW
  4. trans-Golgi network Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Formation of aberrant N-glycan structures.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 624624Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3PRO_0000397935Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi69 – 691N-linked (GlcNAc...)Sequence Analysis
Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence Analysis
Glycosylationi236 – 2361N-linked (GlcNAc...)Sequence Analysis
Glycosylationi377 – 3771N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi428 ↔ 471By similarity
Glycosylationi503 – 5031N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ93Y37.
PRIDEiQ93Y37.

Expressioni

Tissue specificityi

Expressed in flowers, siliques, stems, leaves, roots, stamens and sepals.1 Publication

Gene expression databases

GenevestigatoriQ93Y37.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G30000.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ93Y37.
SMRiQ93Y37. Positions 128-620.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG300315.
HOGENOMiHOG000181987.
InParanoidiQ93Y37.
KOiK01230.
OMAiVHFNLHA.
PhylomeDBiQ93Y37.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERiPTHR11742. PTHR11742. 1 hit.
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.

Sequencei

Sequence statusi: Complete.

Q93Y37-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKSLPYSVK DIHYDNAKFR HRSPLKVFSQ SLLTLSTKRN YASCSTGKFL
60 70 80 90 100
ILILFFGVAC LMLMSKSPNE SGLNEKGKVT FVGGLRLGGL LRKPPRLPPR
110 120 130 140 150
LSPDEGQLRG SSTNGSTISN SDPKWAARQQ SVKEAFDHAW SGYRKYAMGY
160 170 180 190 200
DELMPISQKG VDGLGGLGAT VVDALDTAMI MGLDNIVSEA GSWVETHLLE
210 220 230 240 250
RISQKGQVNL FETTIRVLGG LLSAYHLSGG EQGTVNMTHV GPKPVIYLNI
260 270 280 290 300
AKDLADRLLS AFTSSPTPVP FCDVILHEST AHPAPGGASS TAEVASVQLE
310 320 330 340 350
FNYLSSISGD PKYSTEAMKV LAHIKTLPKT EGLVPIYISP QTGDFVGENI
360 370 380 390 400
RLGSRGDSYY EYLIKVWLQQ GAKLNSNFTY LHDMYIEAMK GVRHLLVQNS
410 420 430 440 450
IPKGLVFVGE LPYGSKGEFS PKMDHLVCFL PGTLALGATK GLTKEQALKE
460 470 480 490 500
NLLSFEDLEN LKLAEDLAKT CFEMYEVTAT GLAPEIAYFH TKDYTEDGLD
510 520 530 540 550
GGNKSSMYAN DIIIKPADRH NLLRPETVES LFVLYRITKD TKYRDQGWQI
560 570 580 590 600
FEAFEKYTKV KSGGYTSLDD VTEVPPHRRD KMETFFLGET LKYLYLLFGD
610 620
DSVIPLDKFV FNTEAHPLPI RRNT
Length:624
Mass (Da):69,069
Last modified:December 1, 2001 - v1
Checksum:iA28B82AD7FAAD29D
GO

Sequence cautioni

The sequence AAG52061.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC022455 Genomic DNA. Translation: AAG52061.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE31166.1.
AY054482 mRNA. Translation: AAK96673.1.
AY093280 mRNA. Translation: AAM13279.1.
PIRiH86423.
RefSeqiNP_564345.1. NM_102740.2.
UniGeneiAt.26476.

Genome annotation databases

EnsemblPlantsiAT1G30000.1; AT1G30000.1; AT1G30000.
GeneIDi839879.
KEGGiath:AT1G30000.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC022455 Genomic DNA. Translation: AAG52061.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE31166.1 .
AY054482 mRNA. Translation: AAK96673.1 .
AY093280 mRNA. Translation: AAM13279.1 .
PIRi H86423.
RefSeqi NP_564345.1. NM_102740.2.
UniGenei At.26476.

3D structure databases

ProteinModelPortali Q93Y37.
SMRi Q93Y37. Positions 128-620.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT1G30000.1-P.

Protein family/group databases

CAZyi GH47. Glycoside Hydrolase Family 47.

Proteomic databases

PaxDbi Q93Y37.
PRIDEi Q93Y37.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G30000.1 ; AT1G30000.1 ; AT1G30000 .
GeneIDi 839879.
KEGGi ath:AT1G30000.

Organism-specific databases

TAIRi AT1G30000.

Phylogenomic databases

eggNOGi NOG300315.
HOGENOMi HOG000181987.
InParanoidi Q93Y37.
KOi K01230.
OMAi VHFNLHA.
PhylomeDBi Q93Y37.

Enzyme and pathway databases

UniPathwayi UPA00378 .
BioCyci ARA:AT1G30000-MONOMER.
Reactomei REACT_190970. ER Quality Control Compartment (ERQC).

Miscellaneous databases

PROi Q93Y37.

Gene expression databases

Genevestigatori Q93Y37.

Family and domain databases

Gene3Di 1.50.10.50. 1 hit.
InterProi IPR001382. Glyco_hydro_47.
[Graphical view ]
PANTHERi PTHR11742. PTHR11742. 1 hit.
Pfami PF01532. Glyco_hydro_47. 1 hit.
[Graphical view ]
PRINTSi PR00747. GLYHDRLASE47.
SUPFAMi SSF48225. SSF48225. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Class I alpha-mannosidases are required for N-glycan processing and root development in Arabidopsis thaliana."
    Liebminger E., Huttner S., Vavra U., Fischl R., Schoberer J., Grass J., Blaukopf C., Seifert G.J., Altmann F., Mach L., Strasser R.
    Plant Cell 21:3850-3867(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, ENZYME REGULATION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiMNS3_ARATH
AccessioniPrimary (citable) accession number: Q93Y37
Secondary accession number(s): Q9C8R9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: December 1, 2001
Last modified: November 26, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3