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Q93Y37

- MNS3_ARATH

UniProt

Q93Y37 - MNS3_ARATH

Protein

Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3

Gene

MNS3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Class I alpha-mannosidase essential for early N-glycan processing. Removes preferentially alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man8GlcNAc2. Involved in root development and cell wall biosynthesis.1 Publication

    Catalytic activityi

    Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

    Cofactori

    Calcium or manganese. Magnesium can be used to a lesser extent.

    Enzyme regulationi

    Inhibited by kifunensine and 1-deoxymannojirimycin, but not by swainsonine.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei212 – 2121Proton donorBy similarity
    Active sitei357 – 3571By similarity
    Active sitei526 – 5261By similarity

    GO - Molecular functioni

    1. alpha-mannosidase activity Source: TAIR
    2. calcium ion binding Source: InterPro
    3. mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. N-glycan processing Source: TAIR
    2. root development Source: TAIR

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Calcium, Magnesium, Manganese

    Enzyme and pathway databases

    BioCyciARA:AT1G30000-MONOMER.
    ReactomeiREACT_190970. ER Quality Control Compartment (ERQC).
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGH47. Glycoside Hydrolase Family 47.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 (EC:3.2.1.113)
    Gene namesi
    Name:MNS3
    Ordered Locus Names:At1g30000
    ORF Names:T1P2.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G30000.

    Subcellular locationi

    Golgi apparatuscis-Golgi network membrane 1 Publication; Single-pass type II membrane protein 1 Publication

    GO - Cellular componenti

    1. endosome Source: TAIR
    2. Golgi apparatus Source: TAIR
    3. integral component of membrane Source: UniProtKB-KW
    4. trans-Golgi network Source: TAIR

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Formation of aberrant N-glycan structures.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 624624Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3PRO_0000397935Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi69 – 691N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi236 – 2361N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi377 – 3771N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi428 ↔ 471By similarity
    Glycosylationi503 – 5031N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ93Y37.
    PRIDEiQ93Y37.

    Expressioni

    Tissue specificityi

    Expressed in flowers, siliques, stems, leaves, roots, stamens and sepals.1 Publication

    Gene expression databases

    GenevestigatoriQ93Y37.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT1G30000.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ93Y37.
    SMRiQ93Y37. Positions 128-620.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4343CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini65 – 624560LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei44 – 6421Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 47 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG300315.
    HOGENOMiHOG000181987.
    InParanoidiQ93Y37.
    KOiK01230.
    OMAiVHFNLHA.
    PhylomeDBiQ93Y37.

    Family and domain databases

    Gene3Di1.50.10.50. 1 hit.
    InterProiIPR001382. Glyco_hydro_47.
    [Graphical view]
    PANTHERiPTHR11742. PTHR11742. 1 hit.
    PfamiPF01532. Glyco_hydro_47. 1 hit.
    [Graphical view]
    PRINTSiPR00747. GLYHDRLASE47.
    SUPFAMiSSF48225. SSF48225. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q93Y37-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKSLPYSVK DIHYDNAKFR HRSPLKVFSQ SLLTLSTKRN YASCSTGKFL    50
    ILILFFGVAC LMLMSKSPNE SGLNEKGKVT FVGGLRLGGL LRKPPRLPPR 100
    LSPDEGQLRG SSTNGSTISN SDPKWAARQQ SVKEAFDHAW SGYRKYAMGY 150
    DELMPISQKG VDGLGGLGAT VVDALDTAMI MGLDNIVSEA GSWVETHLLE 200
    RISQKGQVNL FETTIRVLGG LLSAYHLSGG EQGTVNMTHV GPKPVIYLNI 250
    AKDLADRLLS AFTSSPTPVP FCDVILHEST AHPAPGGASS TAEVASVQLE 300
    FNYLSSISGD PKYSTEAMKV LAHIKTLPKT EGLVPIYISP QTGDFVGENI 350
    RLGSRGDSYY EYLIKVWLQQ GAKLNSNFTY LHDMYIEAMK GVRHLLVQNS 400
    IPKGLVFVGE LPYGSKGEFS PKMDHLVCFL PGTLALGATK GLTKEQALKE 450
    NLLSFEDLEN LKLAEDLAKT CFEMYEVTAT GLAPEIAYFH TKDYTEDGLD 500
    GGNKSSMYAN DIIIKPADRH NLLRPETVES LFVLYRITKD TKYRDQGWQI 550
    FEAFEKYTKV KSGGYTSLDD VTEVPPHRRD KMETFFLGET LKYLYLLFGD 600
    DSVIPLDKFV FNTEAHPLPI RRNT 624
    Length:624
    Mass (Da):69,069
    Last modified:December 1, 2001 - v1
    Checksum:iA28B82AD7FAAD29D
    GO

    Sequence cautioni

    The sequence AAG52061.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC022455 Genomic DNA. Translation: AAG52061.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE31166.1.
    AY054482 mRNA. Translation: AAK96673.1.
    AY093280 mRNA. Translation: AAM13279.1.
    PIRiH86423.
    RefSeqiNP_564345.1. NM_102740.2.
    UniGeneiAt.26476.

    Genome annotation databases

    EnsemblPlantsiAT1G30000.1; AT1G30000.1; AT1G30000.
    GeneIDi839879.
    KEGGiath:AT1G30000.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC022455 Genomic DNA. Translation: AAG52061.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE31166.1 .
    AY054482 mRNA. Translation: AAK96673.1 .
    AY093280 mRNA. Translation: AAM13279.1 .
    PIRi H86423.
    RefSeqi NP_564345.1. NM_102740.2.
    UniGenei At.26476.

    3D structure databases

    ProteinModelPortali Q93Y37.
    SMRi Q93Y37. Positions 128-620.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT1G30000.1-P.

    Protein family/group databases

    CAZyi GH47. Glycoside Hydrolase Family 47.

    Proteomic databases

    PaxDbi Q93Y37.
    PRIDEi Q93Y37.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G30000.1 ; AT1G30000.1 ; AT1G30000 .
    GeneIDi 839879.
    KEGGi ath:AT1G30000.

    Organism-specific databases

    TAIRi AT1G30000.

    Phylogenomic databases

    eggNOGi NOG300315.
    HOGENOMi HOG000181987.
    InParanoidi Q93Y37.
    KOi K01230.
    OMAi VHFNLHA.
    PhylomeDBi Q93Y37.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    BioCyci ARA:AT1G30000-MONOMER.
    Reactomei REACT_190970. ER Quality Control Compartment (ERQC).

    Miscellaneous databases

    PROi Q93Y37.

    Gene expression databases

    Genevestigatori Q93Y37.

    Family and domain databases

    Gene3Di 1.50.10.50. 1 hit.
    InterProi IPR001382. Glyco_hydro_47.
    [Graphical view ]
    PANTHERi PTHR11742. PTHR11742. 1 hit.
    Pfami PF01532. Glyco_hydro_47. 1 hit.
    [Graphical view ]
    PRINTSi PR00747. GLYHDRLASE47.
    SUPFAMi SSF48225. SSF48225. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Class I alpha-mannosidases are required for N-glycan processing and root development in Arabidopsis thaliana."
      Liebminger E., Huttner S., Vavra U., Fischl R., Schoberer J., Grass J., Blaukopf C., Seifert G.J., Altmann F., Mach L., Strasser R.
      Plant Cell 21:3850-3867(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, ENZYME REGULATION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiMNS3_ARATH
    AccessioniPrimary (citable) accession number: Q93Y37
    Secondary accession number(s): Q9C8R9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3