Reviewed, UniProtKB/Swiss-Prot Q93X67 (FABG2_BRANA)

Names and origin

Protein names

Recommended name:
    3-oxoacyl-[acyl-carrier-protein] reductase 2, chloroplastic
    EC=1.1.1.100
Alternative name(s):
    3-ketoacyl-acyl carrier protein reductase 2

Gene names

Name:

bkr2

Organism

Brassica napus (Rape)

Taxonomic identifier

3708 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassica

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subunit structure	Homotetramer By similarity.
Subcellular location	Plastid › chloroplast. Plastid. Note: And non-photosynthetic plastids By similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxoacyl-[acyl-carrier-protein] reductase activity Inferred from electronic annotation. Source: EC NAD or NADH binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 66	66	Chloroplast Potential
	Chain	67 – 328	262	3-oxoacyl-[acyl-carrier-protein] reductase 2, chloroplastic		PRO_0000031978
Regions
	Nucleotide binding	90 – 114	25	NADP By similarity
Sites
	Active site	235	1	Proton acceptor By similarity
	Binding site	222	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q93X67-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: AFB61AF1C665545F	FASTA	328	34,374
	10 20 30 40 50 60 MATTSIAGPR LTSLKTAAGK LGYREICHVR QWAPVHSPMP HFGMLRCGSR RPFATSSSTV 70 80 90 100 110 120 VQAQAQATAA EQSTGEEEAV PKVESPVVVV TGASRGIGKA IALSLGKAGC KVLVNYARSA 130 140 150 160 170 180 KEAEQVSKQI EAYGGQAITF GGDVSKEADV DAMMKTAVDA WGTIDVVVNN AGITRDTLLI 190 200 210 220 230 240 RMKKSQWDEV IDLNLTGVFL CTQAATKIMM KKRKGRIINI ASVVGLIGNI GQANYAAAKA 250 260 270 280 290 300 GVIGFSKTAA REGASRNINV NVVCPGFIAS DMTAKLGEDM EKKILGTIPL GRYGQPEDVA 310 320 GLVEFLALSP AASYITGQAF TIDGGIAI

References

[1]	McDonald F.S., White A.J., Elborough K.M., Slabas A.R. Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Jet neuf. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ243084 mRNA. Translation: CAC41363.1.
3D structure databases
SMR	Q93X67. Positions 85-328.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.100. 393.
Family and domain databases
InterPro	IPR011284. 3oxo_ACP_reduc. IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
TIGRFAMs	TIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FABG2_BRANA

Accession

Primary (citable) accession number: Q93X67

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	December 1, 2001
Last modified:	February 9, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)