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Q93X62

- FABG1_BRANA

UniProt

Q93X62 - FABG1_BRANA

Protein

3-oxoacyl-[acyl-carrier-protein] reductase 1, chloroplastic

Gene

gbkr1

Organism
Brassica napus (Rape)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    (3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei214 – 2141Substrate
    Active sitei227 – 2271Proton acceptor

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi82 – 10625NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity Source: UniProtKB-EC
    2. NAD binding Source: InterPro

    GO - Biological processi

    1. fatty acid biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-oxoacyl-[acyl-carrier-protein] reductase 1, chloroplastic (EC:1.1.1.100)
    Alternative name(s):
    3-ketoacyl-acyl carrier protein reductase 1
    Beta-keto acyl-carrier protein reductase 1
    Gene namesi
    Name:gbkr1
    OrganismiBrassica napus (Rape)
    Taxonomic identifieri3708 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

    Subcellular locationi

    Plastidchloroplast By similarity
    Note: And non-photosynthetic plastids.By similarity

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 6060ChloroplastSequence AnalysisAdd
    BLAST
    Chaini61 – 3202603-oxoacyl-[acyl-carrier-protein] reductase 1, chloroplasticPRO_0000031977Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.

    Structurei

    Secondary structure

    1
    320
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi79 – 824
    Helixi88 – 9912
    Beta strandi103 – 1108
    Helixi112 – 12514
    Beta strandi128 – 1336
    Helixi139 – 15214
    Beta strandi157 – 1615
    Helixi171 – 1733
    Helixi176 – 18611
    Helixi188 – 20417
    Beta strandi207 – 2126
    Helixi216 – 2194
    Helixi225 – 24622
    Turni247 – 2493
    Beta strandi250 – 2578
    Helixi263 – 2664
    Helixi270 – 2778
    Helixi288 – 30013
    Helixi303 – 3064
    Beta strandi311 – 3155
    Turni316 – 3194

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1EDOX-ray2.30A77-320[»]
    2CDHX-ray4.20G/H/I/J/K/L77-320[»]
    ProteinModelPortaliQ93X62.
    SMRiQ93X62. Positions 77-320.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ93X62.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR011284. 3oxo_ACP_reduc.
    IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view]
    PfamiPF00106. adh_short. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000126. 11-beta-HSD1. 1 hit.
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.
    TIGRFAMsiTIGR01830. 3oxo_ACP_reduc. 1 hit.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q93X62-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATTVAATKL TSLKAVKKLG FREIRQVRQW SPLQSAMPHF GMLRCGSRQS    50
    FATSTVVKAQ ATAVEQSTGE AVPKVESPVV VVTGASRGIG KAIALSLGKA 100
    GCKVLVNYAR SAKEAEEVSK QIEAYGGQAI TFGGDVSKEA DVEAMMKTAI 150
    DAWGTIDVVV NNAGITRDTL LIRMKKSQWD EVIDLNLTGV FLCTQAATKI 200
    MMKKRKGRII NIASVVGLIG NIGQANYAAA KAGVIGFSKT AAREGASRNI 250
    NVNVVCPGFI ASDMTAKLGE DMEKKILGTI PLGRYGQPED VAGLVEFLAL 300
    SPAASYITGQ AFTIDGGIAI 320
    Length:320
    Mass (Da):33,671
    Last modified:December 1, 2001 - v1
    Checksum:i3D79CEA41AFFBC49
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ243091 Genomic DNA. Translation: CAC41370.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ243091 Genomic DNA. Translation: CAC41370.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1EDO X-ray 2.30 A 77-320 [» ]
    2CDH X-ray 4.20 G/H/I/J/K/L 77-320 [» ]
    ProteinModelPortali Q93X62.
    SMRi Q93X62. Positions 77-320.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00094 .

    Miscellaneous databases

    EvolutionaryTracei Q93X62.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR011284. 3oxo_ACP_reduc.
    IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view ]
    Pfami PF00106. adh_short. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000126. 11-beta-HSD1. 1 hit.
    PRINTSi PR00081. GDHRDH.
    PR00080. SDRFAMILY.
    TIGRFAMsi TIGR01830. 3oxo_ACP_reduc. 1 hit.
    PROSITEi PS00061. ADH_SHORT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. McDonald F.S., White A.J., Elborough K.M., Slabas A.R.
      Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Jet neuf.
      Tissue: Leaf.
    2. "Crystallization of the NADP-dependent beta-keto acyl-carrier protein reductase from Brassica napus."
      Fisher M., Sedelnikova S.E., Martindale W., Thomas N.C., Simon J.W., Slabas A.R., Rafferty J.B.
      Acta Crystallogr. D 56:86-88(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CRYSTALLIZATION.
    3. "The X-ray structure of Brassica napus beta-keto acyl carrier protein reductase and its implications for substrate binding and catalysis."
      Fisher M., Kroon J.T.M., Martindale W., Stuitje A.R., Slabas A.R., Rafferty J.B.
      Structure 8:339-347(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 77-320.

    Entry informationi

    Entry nameiFABG1_BRANA
    AccessioniPrimary (citable) accession number: Q93X62
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 4, 2005
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3