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Q93X62 (FABG1_BRANA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
3-oxoacyl-[acyl-carrier-protein] reductase 1, chloroplastic

EC=1.1.1.100
Alternative name(s):
3-ketoacyl-acyl carrier protein reductase 1
Beta-keto acyl-carrier protein reductase 1
Gene names
Name:gbkr1
OrganismBrassica napus (Rape)
Taxonomic identifier3708 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Protein attributes

Sequence length320 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

Pathway

Lipid metabolism; fatty acid biosynthesis.

Subunit structure

Homotetramer.

Subcellular location

Plastidchloroplast By similarity. Note: And non-photosynthetic plastids By similarity.

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
   Biological processFatty acid biosynthesis
Fatty acid metabolism
Lipid biosynthesis
Lipid metabolism
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandNADP
   Molecular functionOxidoreductase
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processfatty acid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity

Inferred from electronic annotation. Source: UniProtKB-EC

NAD binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 6060Chloroplast Potential
Chain61 – 3202603-oxoacyl-[acyl-carrier-protein] reductase 1, chloroplastic
PRO_0000031977

Regions

Nucleotide binding82 – 10625NADP By similarity

Sites

Active site2271Proton acceptor
Binding site2141Substrate

Secondary structure

........................................ 320
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q93X62 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 3D79CEA41AFFBC49

FASTA32033,671
        10         20         30         40         50         60 
MATTVAATKL TSLKAVKKLG FREIRQVRQW SPLQSAMPHF GMLRCGSRQS FATSTVVKAQ 

        70         80         90        100        110        120 
ATAVEQSTGE AVPKVESPVV VVTGASRGIG KAIALSLGKA GCKVLVNYAR SAKEAEEVSK 

       130        140        150        160        170        180 
QIEAYGGQAI TFGGDVSKEA DVEAMMKTAI DAWGTIDVVV NNAGITRDTL LIRMKKSQWD 

       190        200        210        220        230        240 
EVIDLNLTGV FLCTQAATKI MMKKRKGRII NIASVVGLIG NIGQANYAAA KAGVIGFSKT 

       250        260        270        280        290        300 
AAREGASRNI NVNVVCPGFI ASDMTAKLGE DMEKKILGTI PLGRYGQPED VAGLVEFLAL 

       310        320 
SPAASYITGQ AFTIDGGIAI 

« Hide

References

[1]McDonald F.S., White A.J., Elborough K.M., Slabas A.R.
Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Jet neuf.
Tissue: Leaf.
[2]"Crystallization of the NADP-dependent beta-keto acyl-carrier protein reductase from Brassica napus."
Fisher M., Sedelnikova S.E., Martindale W., Thomas N.C., Simon J.W., Slabas A.R., Rafferty J.B.
Acta Crystallogr. D 56:86-88(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CRYSTALLIZATION.
[3]"The X-ray structure of Brassica napus beta-keto acyl carrier protein reductase and its implications for substrate binding and catalysis."
Fisher M., Kroon J.T.M., Martindale W., Stuitje A.R., Slabas A.R., Rafferty J.B.
Structure 8:339-347(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 77-320.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ243091 Genomic DNA. Translation: CAC41370.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1EDOX-ray2.30A77-320[»]
ProteinModelPortalQ93X62.
SMRQ93X62. Positions 77-320.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00094.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR011284. 3oxo_ACP_reduc.
IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamPF00106. adh_short. 1 hit.
[Graphical view]
PIRSFPIRSF000126. 11-beta-HSD1. 1 hit.
PRINTSPR00081. GDHRDH.
PR00080. SDRFAMILY.
TIGRFAMsTIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITEPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ93X62.

Entry information

Entry nameFABG1_BRANA
AccessionPrimary (citable) accession number: Q93X62
Entry history
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: December 1, 2001
Last modified: February 19, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways