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Protein

3-oxoacyl-[acyl-carrier-protein] reductase 1, chloroplastic

Gene

gbkr1

Organism
Brassica napus (Rape)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

Pathway:ifatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei214 – 2141Substrate
Active sitei227 – 2271Proton acceptor

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi82 – 10625NADPBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoacyl-[acyl-carrier-protein] reductase 1, chloroplastic (EC:1.1.1.100)
Alternative name(s):
3-ketoacyl-acyl carrier protein reductase 1
Beta-keto acyl-carrier protein reductase 1
Gene namesi
Name:gbkr1
OrganismiBrassica napus (Rape)
Taxonomic identifieri3708 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6060ChloroplastSequence AnalysisAdd
BLAST
Chaini61 – 3202603-oxoacyl-[acyl-carrier-protein] reductase 1, chloroplasticPRO_0000031977Add
BLAST

Interactioni

Subunit structurei

Homotetramer.

Structurei

Secondary structure

1
320
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi79 – 824Combined sources
Helixi88 – 9912Combined sources
Beta strandi103 – 1108Combined sources
Helixi112 – 12514Combined sources
Beta strandi128 – 1336Combined sources
Helixi139 – 15214Combined sources
Beta strandi157 – 1615Combined sources
Helixi171 – 1733Combined sources
Helixi176 – 18611Combined sources
Helixi188 – 20417Combined sources
Beta strandi207 – 2126Combined sources
Helixi216 – 2194Combined sources
Helixi225 – 24622Combined sources
Turni247 – 2493Combined sources
Beta strandi250 – 2578Combined sources
Helixi263 – 2664Combined sources
Helixi270 – 2778Combined sources
Helixi288 – 30013Combined sources
Helixi303 – 3064Combined sources
Beta strandi311 – 3155Combined sources
Turni316 – 3194Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EDOX-ray2.30A77-320[»]
2CDHX-ray4.20G/H/I/J/K/L77-320[»]
ProteinModelPortaliQ93X62.
SMRiQ93X62. Positions 77-320.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ93X62.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR011284. 3oxo_ACP_reduc.
IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
TIGRFAMsiTIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q93X62-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATTVAATKL TSLKAVKKLG FREIRQVRQW SPLQSAMPHF GMLRCGSRQS
60 70 80 90 100
FATSTVVKAQ ATAVEQSTGE AVPKVESPVV VVTGASRGIG KAIALSLGKA
110 120 130 140 150
GCKVLVNYAR SAKEAEEVSK QIEAYGGQAI TFGGDVSKEA DVEAMMKTAI
160 170 180 190 200
DAWGTIDVVV NNAGITRDTL LIRMKKSQWD EVIDLNLTGV FLCTQAATKI
210 220 230 240 250
MMKKRKGRII NIASVVGLIG NIGQANYAAA KAGVIGFSKT AAREGASRNI
260 270 280 290 300
NVNVVCPGFI ASDMTAKLGE DMEKKILGTI PLGRYGQPED VAGLVEFLAL
310 320
SPAASYITGQ AFTIDGGIAI
Length:320
Mass (Da):33,671
Last modified:December 1, 2001 - v1
Checksum:i3D79CEA41AFFBC49
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ243091 Genomic DNA. Translation: CAC41370.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ243091 Genomic DNA. Translation: CAC41370.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EDOX-ray2.30A77-320[»]
2CDHX-ray4.20G/H/I/J/K/L77-320[»]
ProteinModelPortaliQ93X62.
SMRiQ93X62. Positions 77-320.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00094.

Miscellaneous databases

EvolutionaryTraceiQ93X62.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR011284. 3oxo_ACP_reduc.
IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
TIGRFAMsiTIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. McDonald F.S., White A.J., Elborough K.M., Slabas A.R.
    Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Jet neuf.
    Tissue: Leaf.
  2. "Crystallization of the NADP-dependent beta-keto acyl-carrier protein reductase from Brassica napus."
    Fisher M., Sedelnikova S.E., Martindale W., Thomas N.C., Simon J.W., Slabas A.R., Rafferty J.B.
    Acta Crystallogr. D 56:86-88(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CRYSTALLIZATION.
  3. "The X-ray structure of Brassica napus beta-keto acyl carrier protein reductase and its implications for substrate binding and catalysis."
    Fisher M., Kroon J.T.M., Martindale W., Stuitje A.R., Slabas A.R., Rafferty J.B.
    Structure 8:339-347(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 77-320.

Entry informationi

Entry nameiFABG1_BRANA
AccessioniPrimary (citable) accession number: Q93X62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: December 1, 2001
Last modified: April 1, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.