Reviewed, UniProtKB/Swiss-Prot Q93X62 (FABG1_BRANA)

Names and origin

Protein names

Recommended name:
    3-oxoacyl-[acyl-carrier-protein] reductase 1, chloroplastic
    EC=1.1.1.100
Alternative name(s):
    3-ketoacyl-acyl carrier protein reductase 1
    Beta-keto acyl-carrier protein reductase 1

Gene names

Name:

gbkr1

Organism

Brassica napus (Rape)

Taxonomic identifier

3708 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassica

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subunit structure	Homotetramer.
Subcellular location	Plastid › chloroplast By similarity. Note: And non-photosynthetic plastids By similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxoacyl-[acyl-carrier-protein] reductase activity Inferred from electronic annotation. Source: EC NAD or NADH binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 60

60

Chloroplast Potential

Chain

61 – 320

260

3-oxoacyl-[acyl-carrier-protein] reductase 1, chloroplastic

PRO_0000031977

Regions

Nucleotide binding

82 – 106

25

NADP By similarity

Sites

Active site

227

1

Proton acceptor

Binding site

214

1

Substrate

Secondary structure

1

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320

Helix Strand Turn

Details...

Beta strand

79 – 82

4

Helix

88 – 99

12

Beta strand

103 – 110

8

Helix

112 – 125

14

Beta strand

128 – 133

6

Helix

139 – 152

14

Beta strand

157 – 161

5

Helix

171 – 173

3

Helix

176 – 186

11

Helix

188 – 204

17

Beta strand

207 – 212

6

Helix

216 – 219

4

Helix

225 – 246

22

Turn

247 – 249

3

Beta strand

250 – 257

8

Helix

263 – 266

4

Helix

270 – 277

8

Helix

288 – 300

13

Helix

303 – 306

4

Beta strand

311 – 315

5

Turn

316 – 319

4

Sequences

	Sequence		Length	Mass (Da)
	Q93X62-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 3D79CEA41AFFBC49	FASTA	320	33,671
	10 20 30 40 50 60 MATTVAATKL TSLKAVKKLG FREIRQVRQW SPLQSAMPHF GMLRCGSRQS FATSTVVKAQ 70 80 90 100 110 120 ATAVEQSTGE AVPKVESPVV VVTGASRGIG KAIALSLGKA GCKVLVNYAR SAKEAEEVSK 130 140 150 160 170 180 QIEAYGGQAI TFGGDVSKEA DVEAMMKTAI DAWGTIDVVV NNAGITRDTL LIRMKKSQWD 190 200 210 220 230 240 EVIDLNLTGV FLCTQAATKI MMKKRKGRII NIASVVGLIG NIGQANYAAA KAGVIGFSKT 250 260 270 280 290 300 AAREGASRNI NVNVVCPGFI ASDMTAKLGE DMEKKILGTI PLGRYGQPED VAGLVEFLAL 310 320 SPAASYITGQ AFTIDGGIAI

References

[1]	McDonald F.S., White A.J., Elborough K.M., Slabas A.R. Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Jet neuf. Tissue: Leaf.
[2]	"Crystallization of the NADP-dependent beta-keto acyl-carrier protein reductase from Brassica napus." Fisher M., Sedelnikova S.E., Martindale W., Thomas N.C., Simon J.W., Slabas A.R., Rafferty J.B. Acta Crystallogr. D 56:86-88(2000) [PubMed: 10666637] [Abstract] Cited for: CRYSTALLIZATION.
[3]	"The X-ray structure of Brassica napus beta-keto acyl carrier protein reductase and its implications for substrate binding and catalysis." Fisher M., Kroon J.T.M., Martindale W., Stuitje A.R., Slabas A.R., Rafferty J.B. Structure 8:339-347(2000) [PubMed: 10801480] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 77-320.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ243091 Genomic DNA. Translation: CAC41370.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EDO	X-ray	2.30	A	77-320	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

1.1.1.100. 393.

Family and domain databases

InterPro

IPR011284. 3oxo_ACP_reduc.
IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

PANTHER

PTHR19410. ADH_short_C2. 1 hit.

Pfam

PF00106. adh_short. 1 hit.
[Graphical view]

PRINTS

PR00081. GDHRDH.
PR00080. SDRFAMILY.

TIGRFAMs

TIGR01830. 3oxo_ACP_reduc. 1 hit.

PROSITE

PS00061. ADH_SHORT. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

FABG1_BRANA

Accession

Primary (citable) accession number: Q93X62

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	December 1, 2001
Last modified:	February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)