Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q93X60 (Q93X60_CICIN) Unreviewed, UniProtKB/TrEMBL

Last modified November 16, 2011. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesSubmitted name:
Fructan 1-exohydrolase IIa EMBL CAC37922.1
EC=3.2.1.80 EMBL CAC37922.1
Gene names
Name:1-feh IIa EMBL CAC37922.1
OrganismCichorium intybus (Chicory) EMBL CAC37922.1
Taxonomic identifier13427 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Asterales › Asteraceae › Cichorioideae › Cichorieae › Cichoriinae › Cichorium

Protein attributes

Sequence length581 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the glycosyl hydrolase 32 family. RuleBase RU000449

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3838 Potential EMBL CAC37922.1
Chain39 – 581543fructan 1-exohydrolase IIa EMBL CAC37922.1
PRO_5000066514

Regions

Region59 – 602Fructose binding PDB 2ADE
Region120 – 1212Fructose binding PDB 2ADE
Region120 – 1212Sucrose binding PDB 2ADD
Region184 – 1852Fructose binding PDB 2ADE
Region184 – 1852Sucrose binding PDB 2ADD

Sites

Binding site591Sucrose PDB 2ADD
Binding site761Fructose PDB 2ADE
Binding site761Sucrose PDB 2ADD
Binding site1451Sucrose PDB 2ADD
Binding site2391Fructose PDB 2ADE
Binding site2391Sucrose PDB 2ADD

Amino acid modifications

Glycosylation1541N-linked (GlcNAc...) PDB 2ADE PDB 2AEY PDB 2ADD PDB 2AEZ PDB 1ST8
Glycosylation5511N-linked (GlcNAc...) PDB 2ADE PDB 2AEY PDB 2ADD PDB 2AEZ

Sequences

Sequence LengthMass (Da)Tools
Q93X60 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 8E5233D54C3B98BB

FASTA58165,279
        10         20         30         40         50         60 
MKKSLSSFIV LCFLVIILET GRVKATSRNL NDVIMLANQQ IEQPYRTGYH FQPPSNWMND 

        70         80         90        100        110        120 
PNGPMLYQGV YHFFYQYNPY AATFGDVIIW GHAVSYDLVN WIHLDPAIYP TQEADSKSCW 

       130        140        150        160        170        180 
SGSATILPGN IPAMLYTGSD SKSRQVQDLA WPKNLSDPFL REWVKHPKNP LITPPEGVKD 

       190        200        210        220        230        240 
DCFRDPSTAW LGPDGVWRIV VGGDRDNNGM AFLYQSTDFV NWKRYDQPLS SADATGTWEC 

       250        260        270        280        290        300 
PDFYPVPLNS TNGLDTSVYG GSVRHVMKAG FEGHDWYTIG TYSPDRENFL PQNGLSLTGS 

       310        320        330        340        350        360 
TLDLRYDYGQ FYASKSFFDD AKNRRVLWAW VPETDSQADD IEKGWAGLQS FPRALWIDRN 

       370        380        390        400        410        420 
GKQLIQWPVE EIEELRQNQV NLQNKNLKPG SVLEIHGIAA SQADVTISFK LEGLKEAEVL 

       430        440        450        460        470        480 
DTTLVDPQAL CNERGASSRG ALGPFGLLAM ASKDLKEQSA IFFRVFQNQL GRYSVLMCSD 

       490        500        510        520        530        540 
LSRSTVRSNI DTTSYGAFVD IDPRSEEISL RNLIDHSIIE SFGAGGKTCI TSRIYPKFVN 

       550        560        570        580 
NEEAHLFVFN NGTQNVKISE MSAWSMKNAK FVVDQSVKSA A

« Hide

References

[1]"Expression analysis of a chicory fructan 1-exohydrolase gene reveals complex regulation by cold."
Michiels A., Van Laere A., Van den Ende W., Tucker M.
J. Exp. Bot. 55:1325-1333(2004) [PubMed: 15133058] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[2]"X-ray diffraction structure of a plant glycosyl hydrolase family 32 protein: fructan 1-exohydrolase IIa of Cichorium intybus."
Verhaest M., Van den Ende W., Roy K.L., De Ranter C.J., Laere A.V., Rabijns A.
Plant J. 41:400-411(2005) [PubMed: 15659099] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) OF 39-581, GLYCOSYLATION AT ASN-154.
[3]"Insights into the fine architecture of the active site of chicory fructan 1-exohydrolase: 1-kestose as substrate vs sucrose as inhibitor."
Verhaest M., Lammens W., Le Roy K., De Ranter C.J., Van Laere A., Rabijns A., Van den Ende W.
New Phytol. 174:90-100(2007) [PubMed: 17335500] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 39-581 IN COMPLEX WITH FRUCTOSE AND SUCROSE, GLYCOSYLATION AT ASN-154 AND ASN-551.
[4]"Defoliation induces fructan 1-exohydrolase II in Witloof chicory roots. Cloning and purification of two isoforms, fructan 1-exohydrolase IIa and fructan 1-exohydrolase IIb. Mass fingerprint of the fructan 1-exohydrolase II enzymes."
Van den Ende W., Michiels A., Van Wonterghem D., Clerens S.P., De Roover J., Van Laere A.J.
Plant Physiol. 126:1186-1195(2001) [PubMed: 11457968] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Tissue: Root EMBL CAC37922.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY323935 Genomic DNA. Translation: AAP85536.1.
AJ295033 mRNA. Translation: CAC37922.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1ST8X-ray2.35A39-581[»]
2ADDX-ray2.50A39-581[»]
2ADEX-ray2.65A39-581[»]
2AEYX-ray3.27A39-581[»]
2AEZX-ray3.05A39-581[»]
ProteinModelPortalQ93X60.
SMRQ93X60. Positions 40-576.
ModBaseSearch...

Protein family/group databases

CAZyGH32. Glycoside Hydrolase Family 32.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR008985. ConA-like_lec_gl.
IPR001362. Glyco_hydro_32.
IPR018053. Glyco_hydro_32_AS.
IPR013189. Glyco_hydro_32_C.
IPR013148. Glyco_hydro_32_N.
IPR023296. Glyco_hydro_43_beta-prop.
[Graphical view]
Gene3DG3DSA:2.115.10.20. Glyco_hydro_43_beta-prop. 1 hit.
PfamPF08244. Glyco_hydro_32C. 1 hit.
PF00251. Glyco_hydro_32N. 1 hit.
[Graphical view]
SMARTSM00640. Glyco_32. 1 hit.
[Graphical view]
SUPFAMSSF49899. ConA_like_lec_gl. 1 hit.
SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
PROSITEPS00609. GLYCOSYL_HYDROL_F32. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ93X60_CICIN
AccessionPrimary (citable) accession number: Q93X60
Entry history
Integrated into UniProtKB/TrEMBL: December 1, 2001
Last sequence update: December 1, 2001
Last modified: November 16, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info