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Protein

Cysteine desulfurase 1, chloroplastic

Gene

NFS2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L-selenocystine to produce L-alanine (PubMed:12033984, PubMed:16455656). Supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters (PubMed:15480755, PubMed:17372218). Required for the maturation of all plastidic Fe-S proteins and, thus, essential for plant growth (PubMed:17372218).4 Publications

Catalytic activityi

L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor.4 Publications
L-selenocysteine + reduced acceptor = selenide + L-alanine + acceptor.2 Publications

Cofactori

Enzyme regulationi

The cysteine desulfurase activity is stimulated over 40-fold upon complex formation with SUFE1 (PubMed:16437155, PubMed:16455656).2 Publications

Kineticsi

  1. KM=0.11 mM for L-Cysteine (at 37 degrees Celsius and pH 7.9)1 Publication
  2. KM=2.7 mM for L-Selenocysteine (at 37 degrees Celsius and pH 7.9)1 Publication
  1. Vmax=0.0013 µmol/min/mg enzyme with L-cysteine as substrate1 Publication
  2. Vmax=2.44 µmol/min/mg enzyme with L-Selenocysteine as substrate1 Publication

pH dependencei

Optimum pH is 7-9.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei418Cysteine persulfide intermediateBy similarity1

GO - Molecular functioni

  • cysteine desulfurase activity Source: TAIR
  • pyridoxal phosphate binding Source: InterPro
  • selenocysteine lyase activity Source: TAIR

GO - Biological processi

  • cysteine metabolic process Source: InterPro
  • iron incorporation into metallo-sulfur cluster Source: TAIR
  • response to selenium ion Source: TAIR
  • selenium compound metabolic process Source: TAIR
  • sulfur compound metabolic process Source: TAIR

Keywordsi

Molecular functionLyase, Transferase
LigandPyridoxal phosphate

Enzyme and pathway databases

BRENDAi2.8.1.7 399
SABIO-RKiQ93WX6

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine desulfurase 1, chloroplastic1 Publication (EC:2.8.1.74 Publications)
Alternative name(s):
NIFS-like protein 11 Publication
Short name:
CpNifS1
Plastid sufS-like protein
Protein AtCpNifS
Selenocysteine lyase (EC:4.4.1.162 Publications)
Gene namesi
Name:NFS21 Publication
Synonyms:CpNIFS1 Publication, CpNIFS1
Ordered Locus Names:At1g08490Imported
ORF Names:T27G7.17Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G08490
TAIRilocus:2201856 AT1G08490

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Biotechnological usei

NFS2-overexpressing transgenic plants have enhanced ability to tolerate and accumulate Se and may be used in phytoremediation.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi388C → S: Total loss of the Cys desulfurase activity, but only 20% decrease of the SeCys lyase activity. 1 Publication1
Mutagenesisi418C → S: Loss of catalytic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 35ChloroplastAdd BLAST35
ChainiPRO_000025065236 – 463Cysteine desulfurase 1, chloroplasticAdd BLAST428

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei275N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiQ93WX6

Expressioni

Tissue specificityi

Ubiquitous.2 Publications

Gene expression databases

ExpressionAtlasiQ93WX6 baseline and differential
GenevisibleiQ93WX6 AT

Interactioni

Subunit structurei

Heterotetramer with SUFE1 (PubMed:16455656). Interacts with QS (PubMed:18978034). Interacts with SUFE1 (PubMed:16437155, PubMed:18978034).3 Publications

Protein-protein interaction databases

BioGridi22611, 2 interactors
STRINGi3702.AT1G08490.1

Structurei

Secondary structure

1463
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi53 – 57Combined sources5
Helixi58 – 60Combined sources3
Helixi62 – 64Combined sources3
Beta strandi65 – 68Combined sources4
Turni78 – 80Combined sources3
Helixi86 – 98Combined sources13
Helixi109 – 128Combined sources20
Helixi134 – 136Combined sources3
Beta strandi137 – 142Combined sources6
Helixi143 – 153Combined sources11
Helixi155 – 158Combined sources4
Beta strandi164 – 168Combined sources5
Helixi173 – 186Combined sources14
Beta strandi189 – 193Combined sources5
Beta strandi199 – 201Combined sources3
Helixi203 – 207Combined sources5
Beta strandi214 – 222Combined sources9
Turni224 – 226Combined sources3
Helixi232 – 240Combined sources9
Turni241 – 243Combined sources3
Beta strandi245 – 249Combined sources5
Turni251 – 256Combined sources6
Helixi261 – 264Combined sources4
Beta strandi267 – 272Combined sources6
Beta strandi283 – 287Combined sources5
Helixi289 – 294Combined sources6
Beta strandi301 – 308Combined sources8
Beta strandi313 – 315Combined sources3
Helixi320 – 322Combined sources3
Helixi329 – 345Combined sources17
Helixi347 – 366Combined sources20
Beta strandi371 – 373Combined sources3
Beta strandi380 – 382Combined sources3
Beta strandi386 – 392Combined sources7
Helixi397 – 408Combined sources12
Beta strandi413 – 415Combined sources3
Helixi420 – 425Combined sources6
Beta strandi431 – 435Combined sources5
Helixi442 – 460Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4Q75X-ray1.71A/B36-463[»]
4Q76X-ray1.90A/B36-463[»]
ProteinModelPortaliQ93WX6
SMRiQ93WX6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410INGQ Eukaryota
COG0520 LUCA
HOGENOMiHOG000017511
InParanoidiQ93WX6
KOiK11717
OMAiSGHKMCA
OrthoDBiEOG09360CYM
PhylomeDBiQ93WX6

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR000192 Aminotrans_V_dom
IPR010970 Cys_dSase_SufS
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00266 Aminotran_5, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR01979 sufS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q93WX6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGVAMKLPS FPNAISIGHR SFSRVRCSSS LSVCSAAAAS SATISTDSES
60 70 80 90 100
VSLGHRVRKD FRILHQEVNG SKLVYLDSAA TSQKPAAVLD ALQNYYEFYN
110 120 130 140 150
SNVHRGIHYL SAKATDEFEL ARKKVARFIN ASDSREIVFT RNATEAINLV
160 170 180 190 200
AYSWGLSNLK PGDEVILTVA EHHSCIVPWQ IVSQKTGAVL KFVTLNEDEV
210 220 230 240 250
PDINKLRELI SPKTKLVAVH HVSNVLASSL PIEEIVVWAH DVGAKVLVDA
260 270 280 290 300
CQSVPHMVVD VQKLNADFLV ASSHKMCGPT GIGFLYGKSD LLHSMPPFLG
310 320 330 340 350
GGEMISDVFL DHSTYAEPPS RFEAGTPAIG EAIALGAAVD YLSGIGMPKI
360 370 380 390 400
HEYEVEIGKY LYEKLSSLPD VRIYGPRPSE SVHRGALCSF NVEGLHPTDL
410 420 430 440 450
ATFLDQQHGV AIRSGHHCAQ PLHRYLGVNA SARASLYFYN TKDDVDAFIV
460
ALADTVSFFN SFK
Length:463
Mass (Da):50,485
Last modified:December 1, 2001 - v1
Checksum:i7A00460377E01067
GO

Sequence cautioni

The sequence AAF22900 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY078068 mRNA Translation: AAL79956.1
AF419347 mRNA Translation: AAL14994.1
AC006932 Genomic DNA Translation: AAF22900.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE28298.1
AY094425 mRNA Translation: AAM19798.1
AY149950 mRNA Translation: AAN31104.1
RefSeqiNP_172325.2, NM_100722.5
UniGeneiAt.22935
At.26502

Genome annotation databases

EnsemblPlantsiAT1G08490.1; AT1G08490.1; AT1G08490
GeneIDi837370
GrameneiAT1G08490.1; AT1G08490.1; AT1G08490
KEGGiath:AT1G08490

Similar proteinsi

Entry informationi

Entry nameiCNIF1_ARATH
AccessioniPrimary (citable) accession number: Q93WX6
Secondary accession number(s): Q9SJE3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: December 1, 2001
Last modified: April 25, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health