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Protein

Cysteine desulfurase 1, chloroplastic

Gene

NFS2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L-selenocystine to produce L-alanine (PubMed:12033984, PubMed:16455656). Supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters (PubMed:15480755, PubMed:17372218). Required for the maturation of all plastidic Fe-S proteins and, thus, essential for plant growth (PubMed:17372218).4 Publications

Catalytic activityi

L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor.4 Publications
L-selenocysteine + reduced acceptor = selenide + L-alanine + acceptor.2 Publications

Cofactori

Enzyme regulationi

The cysteine desulfurase activity is stimulated over 40-fold upon complex formation with SUFE1 (PubMed:16437155, PubMed:16455656).2 Publications

Kineticsi

  1. KM=0.11 mM for L-Cysteine (at 37 degrees Celsius and pH 7.9)1 Publication
  2. KM=2.7 mM for L-Selenocysteine (at 37 degrees Celsius and pH 7.9)1 Publication
  1. Vmax=0.0013 µmol/min/mg enzyme with L-cysteine as substrate1 Publication
  2. Vmax=2.44 µmol/min/mg enzyme with L-Selenocysteine as substrate1 Publication

pH dependencei

Optimum pH is 7-9.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei418Cysteine persulfide intermediateBy similarity1

GO - Molecular functioni

  • cysteine desulfurase activity Source: TAIR
  • pyridoxal phosphate binding Source: InterPro
  • selenocysteine lyase activity Source: TAIR

GO - Biological processi

  • cysteine metabolic process Source: InterPro
  • iron incorporation into metallo-sulfur cluster Source: TAIR
  • response to selenium ion Source: TAIR
  • selenium compound metabolic process Source: TAIR
  • sulfur compound metabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BRENDAi2.8.1.7. 399.
SABIO-RKQ93WX6.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine desulfurase 1, chloroplastic1 Publication (EC:2.8.1.74 Publications)
Alternative name(s):
NIFS-like protein 11 Publication
Short name:
CpNifS1
Plastid sufS-like protein
Protein AtCpNifS
Selenocysteine lyase (EC:4.4.1.162 Publications)
Gene namesi
Name:NFS21 Publication
Synonyms:CpNIFS1 Publication, CpNIFS1
Ordered Locus Names:At1g08490Imported
ORF Names:T27G7.17Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G08490.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast stroma Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Biotechnological usei

NFS2-overexpressing transgenic plants have enhanced ability to tolerate and accumulate Se and may be used in phytoremediation.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi388C → S: Total loss of the Cys desulfurase activity, but only 20% decrease of the SeCys lyase activity. 1 Publication1
Mutagenesisi418C → S: Loss of catalytic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 35ChloroplastAdd BLAST35
ChainiPRO_000025065236 – 463Cysteine desulfurase 1, chloroplasticAdd BLAST428

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei275N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiQ93WX6.

Expressioni

Tissue specificityi

Ubiquitous.2 Publications

Gene expression databases

GenevisibleiQ93WX6. AT.

Interactioni

Subunit structurei

Heterotetramer with SUFE1 (PubMed:16455656). Interacts with QS (PubMed:18978034). Interacts with SUFE1 (PubMed:16437155, PubMed:18978034).3 Publications

Protein-protein interaction databases

BioGridi22611. 2 interactors.
STRINGi3702.AT1G08490.1.

Structurei

Secondary structure

1463
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi53 – 57Combined sources5
Helixi58 – 60Combined sources3
Helixi62 – 64Combined sources3
Beta strandi65 – 68Combined sources4
Turni78 – 80Combined sources3
Helixi86 – 98Combined sources13
Helixi109 – 128Combined sources20
Helixi134 – 136Combined sources3
Beta strandi137 – 142Combined sources6
Helixi143 – 153Combined sources11
Helixi155 – 158Combined sources4
Beta strandi164 – 168Combined sources5
Helixi173 – 186Combined sources14
Beta strandi189 – 193Combined sources5
Beta strandi199 – 201Combined sources3
Helixi203 – 207Combined sources5
Beta strandi214 – 222Combined sources9
Turni224 – 226Combined sources3
Helixi232 – 240Combined sources9
Turni241 – 243Combined sources3
Beta strandi245 – 249Combined sources5
Turni251 – 256Combined sources6
Helixi261 – 264Combined sources4
Beta strandi267 – 272Combined sources6
Helixi273 – 275Combined sources3
Beta strandi283 – 287Combined sources5
Helixi289 – 294Combined sources6
Beta strandi301 – 308Combined sources8
Beta strandi313 – 315Combined sources3
Helixi320 – 322Combined sources3
Helixi329 – 345Combined sources17
Helixi347 – 366Combined sources20
Beta strandi371 – 373Combined sources3
Beta strandi380 – 382Combined sources3
Beta strandi386 – 392Combined sources7
Helixi397 – 408Combined sources12
Beta strandi413 – 415Combined sources3
Helixi420 – 425Combined sources6
Beta strandi431 – 435Combined sources5
Helixi442 – 460Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4Q75X-ray1.71A/B36-463[»]
4Q76X-ray1.90A/B36-463[»]
ProteinModelPortaliQ93WX6.
SMRiQ93WX6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410INGQ. Eukaryota.
COG0520. LUCA.
HOGENOMiHOG000017511.
InParanoidiQ93WX6.
KOiK11717.
OMAiMIDFVGL.
OrthoDBiEOG09360CYM.
PhylomeDBiQ93WX6.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR010970. Cys_dSase_SufS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01979. sufS. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q93WX6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGVAMKLPS FPNAISIGHR SFSRVRCSSS LSVCSAAAAS SATISTDSES
60 70 80 90 100
VSLGHRVRKD FRILHQEVNG SKLVYLDSAA TSQKPAAVLD ALQNYYEFYN
110 120 130 140 150
SNVHRGIHYL SAKATDEFEL ARKKVARFIN ASDSREIVFT RNATEAINLV
160 170 180 190 200
AYSWGLSNLK PGDEVILTVA EHHSCIVPWQ IVSQKTGAVL KFVTLNEDEV
210 220 230 240 250
PDINKLRELI SPKTKLVAVH HVSNVLASSL PIEEIVVWAH DVGAKVLVDA
260 270 280 290 300
CQSVPHMVVD VQKLNADFLV ASSHKMCGPT GIGFLYGKSD LLHSMPPFLG
310 320 330 340 350
GGEMISDVFL DHSTYAEPPS RFEAGTPAIG EAIALGAAVD YLSGIGMPKI
360 370 380 390 400
HEYEVEIGKY LYEKLSSLPD VRIYGPRPSE SVHRGALCSF NVEGLHPTDL
410 420 430 440 450
ATFLDQQHGV AIRSGHHCAQ PLHRYLGVNA SARASLYFYN TKDDVDAFIV
460
ALADTVSFFN SFK
Length:463
Mass (Da):50,485
Last modified:December 1, 2001 - v1
Checksum:i7A00460377E01067
GO

Sequence cautioni

The sequence AAF22900 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY078068 mRNA. Translation: AAL79956.1.
AF419347 mRNA. Translation: AAL14994.1.
AC006932 Genomic DNA. Translation: AAF22900.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28298.1.
AY094425 mRNA. Translation: AAM19798.1.
AY149950 mRNA. Translation: AAN31104.1.
RefSeqiNP_172325.2. NM_100722.5.
UniGeneiAt.22935.
At.26502.

Genome annotation databases

EnsemblPlantsiAT1G08490.1; AT1G08490.1; AT1G08490.
GeneIDi837370.
GrameneiAT1G08490.1; AT1G08490.1; AT1G08490.
KEGGiath:AT1G08490.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY078068 mRNA. Translation: AAL79956.1.
AF419347 mRNA. Translation: AAL14994.1.
AC006932 Genomic DNA. Translation: AAF22900.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28298.1.
AY094425 mRNA. Translation: AAM19798.1.
AY149950 mRNA. Translation: AAN31104.1.
RefSeqiNP_172325.2. NM_100722.5.
UniGeneiAt.22935.
At.26502.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4Q75X-ray1.71A/B36-463[»]
4Q76X-ray1.90A/B36-463[»]
ProteinModelPortaliQ93WX6.
SMRiQ93WX6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22611. 2 interactors.
STRINGi3702.AT1G08490.1.

Proteomic databases

PaxDbiQ93WX6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G08490.1; AT1G08490.1; AT1G08490.
GeneIDi837370.
GrameneiAT1G08490.1; AT1G08490.1; AT1G08490.
KEGGiath:AT1G08490.

Organism-specific databases

TAIRiAT1G08490.

Phylogenomic databases

eggNOGiENOG410INGQ. Eukaryota.
COG0520. LUCA.
HOGENOMiHOG000017511.
InParanoidiQ93WX6.
KOiK11717.
OMAiMIDFVGL.
OrthoDBiEOG09360CYM.
PhylomeDBiQ93WX6.

Enzyme and pathway databases

BRENDAi2.8.1.7. 399.
SABIO-RKQ93WX6.

Miscellaneous databases

PROiQ93WX6.

Gene expression databases

GenevisibleiQ93WX6. AT.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR010970. Cys_dSase_SufS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01979. sufS. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCNIF1_ARATH
AccessioniPrimary (citable) accession number: Q93WX6
Secondary accession number(s): Q9SJE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.