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Q93WK5 (APRR7_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Two-component response regulator-like APRR7
Alternative name(s):
Pseudo-response regulator 7
Gene names
Name:APRR7
Ordered Locus Names:At5g02810
ORF Names:F9G14_120
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length727 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional repressor of CCA1 and LHY, and positive regulator of LWD1 and LWD2 expression. Represses the expression of other clock proteins and master regulators of plant growth, development and response to abiotic stress. Involved in the positive and negative feedback loops of the circadian clock. Controls photoperiodic flowering response and temperature compensation. Expression of several members of the ARR-like family is controlled by circadian rhythm. APRR9, APRR7, and APRR5 coordinately act on the upstream region of the target genes to repress their expression from noon until midnight. The particular coordinated sequential expression of APRR9, APRR7, APRR5, APRR3 and APPR1 result to circadian waves that may be at the basis of the endogenous circadian clock. Ref.1 Ref.6 Ref.8 Ref.9 Ref.10 Ref.12 Ref.14 Ref.15

Subcellular location

Nucleus Ref.6.

Induction

Up-regulated by heat-shock and light at dawn, and down-regulated by the sugars produced by photosynthesis. Ref.11 Ref.13 Ref.15

Post-translational modification

Phosphorylated. Phosphorylation varies throughout the diurnal cycle. Ref.7

Disruption phenotype

Insensitive to the effect of sucrose on the circadian period. Ref.15

Miscellaneous

Regulated at the level of mRNA maturation and alternative splicing by SKIP (Ref.11). The expression of APRR9, APRR7, and APRR5 requires the presence of LWD1 and/or LWD2, indicating the existence of a positive feedback loop within the circadian clock (Ref.10).

Sequence similarities

Belongs to the ARR-like family.

Contains 1 CCT domain.

Contains 1 response regulatory domain.

Caution

Lacks the phospho-accepting Asp (here Glu-130), present in the receiver domain, which is one of the conserved features of two-component response regulators (ARRs) family.

Sequence caution

The sequence CAB86035.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 727727Two-component response regulator-like APRR7
PRO_0000081439

Regions

Domain79 – 197119Response regulatory
Domain669 – 71143CCT
Compositional bias202 – 25958Ser-rich
Compositional bias608 – 65851Gly-rich

Sequences

Sequence LengthMass (Da)Tools
Q93WK5 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 13057E6AE6E50C00

FASTA72779,663
        10         20         30         40         50         60 
MNANEEGEGS RYPITDRKTG ETKFDRVESR TEKHSEEEKT NGITMDVRNG SSGGLQIPLS 

        70         80         90        100        110        120 
QQTAATVCWE RFLHVRTIRV LLVENDDCTR YIVTALLRNC SYEVVEASNG IQAWKVLEDL 

       130        140        150        160        170        180 
NNHIDIVLTE VIMPYLSGIG LLCKILNHKS RRNIPVIMMS SHDSMGLVFK CLSKGAVDFL 

       190        200        210        220        230        240 
VKPIRKNELK ILWQHVWRRC QSSSGSGSES GTHQTQKSVK SKSIKKSDQD SGSSDENENG 

       250        260        270        280        290        300 
SIGLNASDGS SDGSGAQSSW TKKAVDVDDS PRAVSLWDRV DSTCAQVVHS NPEFPSNQLV 

       310        320        330        340        350        360 
APPAEKETQE HDDKFEDVTM GRDLEISIRR NCDLALEPKD EPLSKTTGIM RQDNSFEKSS 

       370        380        390        400        410        420 
SKWKMKVGKG PLDLSSESPS SKQMHEDGGS SFKAMSSHLQ DNREPEAPNT HLKTLDTNEA 

       430        440        450        460        470        480 
SVKISEELMH VEHSSKRHRG TKDDGTLVRD DRNVLRRSEG SAFSRYNPAS NANKISGGNL 

       490        500        510        520        530        540 
GSTSLQDNNS QDLIKKTEAA YDCHSNMNES LPHNHRSHVG SNNFDMSSTT ENNAFTKPGA 

       550        560        570        580        590        600 
PKVSSAGSSS VKHSSFQPLP CDHHNNHASY NLVHVAERKK LPPQCGSSNV YNETIEGNNN 

       610        620        630        640        650        660 
TVNYSVNGSV SGSGHGSNGP YGSSNGMNAG GMNMGSDNGA GKNGNGDGSG SGSGSGSGNL 

       670        680        690        700        710        720 
ADENKISQRE AALTKFRQKR KERCFRKKVR YQSRKKLAEQ RPRVRGQFVR KTAAATDDND 


IKNIEDS 

« Hide

References

« Hide 'large scale' references
[1]"Circadian waves of expression of the APRR1/TOC1 family of pseudo-response regulators in Arabidopsis thaliana: insight into the plant circadian clock."
Matsushika A., Makino S., Kojima M., Mizuno T.
Plant Cell Physiol. 41:1002-1012(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Genes encoding pseudo-response regulators: insight into His-to-Asp phosphorelay and circadian rhythm in Arabidopsis thaliana."
Makino S., Kiba T., Imamura A., Hanaki N., Nakamura A., Suzuki T., Taniguchi M., Ueguchi C., Sugiyama T., Mizuno T.
Plant Cell Physiol. 41:791-803(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[6]"Arabidopsis PSEUDO-RESPONSE REGULATOR 7 is a signaling intermediate in phytochrome-regulated seedling deetiolation and phasing of the circadian clock."
Kaczorowski K.A., Quail P.H.
Plant Cell 15:2654-2665(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]"Post-translational regulation of the Arabidopsis circadian clock through selective proteolysis and phosphorylation of pseudo-response regulator proteins."
Fujiwara S., Wang L., Han L., Suh S.-S., Salome P.A., McClung C.R., Somers D.E.
J. Biol. Chem. 283:23073-23083(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION.
[8]"PSEUDO-RESPONSE REGULATORS 9, 7, and 5 are transcriptional repressors in the Arabidopsis circadian clock."
Nakamichi N., Kiba T., Henriques R., Mizuno T., Chua N.H., Sakakibara H.
Plant Cell 22:594-605(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"The role of the Arabidopsis morning loop components CCA1, LHY, PRR7, and PRR9 in temperature compensation."
Salome P.A., Weigel D., McClung C.R.
Plant Cell 22:3650-3661(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"LIGHT-REGULATED WD1 and PSEUDO-RESPONSE REGULATOR9 form a positive feedback regulatory loop in the Arabidopsis circadian clock."
Wang Y., Wu J.F., Nakamichi N., Sakakibara H., Nam H.G., Wu S.H.
Plant Cell 23:486-498(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"SKIP is a component of the spliceosome linking alternative splicing and the circadian clock in Arabidopsis."
Wang X., Wu F., Xie Q., Wang H., Wang Y., Yue Y., Gahura O., Ma S., Liu L., Cao Y., Jiao Y., Puta F., McClung C.R., Xu X., Ma L.
Plant Cell 24:3278-3295(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: REGULATION.
[12]"Transcriptional repressor PRR5 directly regulates clock-output pathways."
Nakamichi N., Kiba T., Kamioka M., Suzuki T., Yamashino T., Higashiyama T., Sakakibara H., Mizuno T.
Proc. Natl. Acad. Sci. U.S.A. 109:17123-17128(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[13]"Heat shock-induced fluctuations in clock and light signaling enhance phytochrome B-mediated Arabidopsis deetiolation."
Karayekov E., Sellaro R., Legris M., Yanovsky M.J., Casal J.J.
Plant Cell 25:2892-2906(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY HEAT-SHOCK.
[14]"Direct regulation of abiotic responses by the Arabidopsis circadian clock component PRR7."
Liu T., Carlsson J., Takeuchi T., Newton L., Farre E.M.
Plant J. 76:101-114(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[15]"Photosynthetic entrainment of the Arabidopsis thaliana circadian clock."
Haydon M.J., Mielczarek O., Robertson F.C., Hubbard K.E., Webb A.A.
Nature 502:689-692(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, INDUCTION BY LIGHT AND SUCROSE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB046954 mRNA. Translation: BAB13742.1.
AL162973 Genomic DNA. Translation: CAB86035.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED90520.1.
AY039943 mRNA. Translation: AAK64047.1.
AY142560 mRNA. Translation: AAN13129.1.
PIRT48302.
RefSeqNP_568107.1. NM_120359.2.
UniGeneAt.20552.

3D structure databases

ProteinModelPortalQ93WK5.
SMRQ93WK5. Positions 77-232.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid17069. 9 interactions.
STRING3702.AT5G02810.1-P.

Proteomic databases

PaxDbQ93WK5.
PRIDEQ93WK5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G02810.1; AT5G02810.1; AT5G02810.
GeneID831793.
KEGGath:AT5G02810.

Organism-specific databases

GeneFarm4348. 437.
TAIRAT5G02810.

Phylogenomic databases

eggNOGCOG3437.
HOGENOMHOG000034018.
InParanoidQ93WK5.
KOK12129.
OMAIGAPRNS.
PhylomeDBQ93WK5.
ProtClustDBCLSN2917538.

Gene expression databases

GenevestigatorQ93WK5.

Family and domain databases

InterProIPR010402. CCT_domain.
IPR011006. CheY-like_superfamily.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamPF06203. CCT. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
SMARTSM00448. REC. 1 hit.
[Graphical view]
SUPFAMSSF52172. SSF52172. 1 hit.
PROSITEPS51017. CCT. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAPRR7_ARATH
AccessionPrimary (citable) accession number: Q93WK5
Secondary accession number(s): Q9LZ04
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names