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Protein

Monodehydroascorbate reductase 2

Gene

MDAR2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of monodehydroascorbate to ascorbate, oxidizing NADH in the process.1 Publication

Catalytic activityi

NADH + 2 monodehydroascorbate = NAD+ + 2 ascorbate.Curated

Cofactori

FADBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei41FADBy similarity1
Binding sitei48FADBy similarity1
Binding sitei53FADBy similarity1
Binding sitei96FAD; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei196NADBy similarity1
Binding sitei202NADBy similarity1
Binding sitei202NADPBy similarity1
Binding sitei261NAD; via amide nitrogenBy similarity1
Binding sitei261NADP; via amide nitrogenBy similarity1
Binding sitei298FADBy similarity1
Binding sitei316FAD; via amide nitrogenBy similarity1
Binding sitei320AscorbateBy similarity1
Binding sitei349FAD; via carbonyl oxygenBy similarity1
Binding sitei349NAD; via carbonyl oxygenBy similarity1
Binding sitei349NADP; via carbonyl oxygenBy similarity1
Binding sitei351AscorbateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 17FADBy similarity4
Nucleotide bindingi147 – 148FADBy similarity2
Nucleotide bindingi172 – 178NADBy similarity7
Nucleotide bindingi174 – 178NADPBy similarity5
Nucleotide bindingi314 – 315NADBy similarity2
Nucleotide bindingi314 – 315NADPBy similarity2

GO - Molecular functioni

  • flavin adenine dinucleotide binding Source: InterPro
  • monodehydroascorbate reductase (NADH) activity Source: TAIR

GO - Biological processi

  • response to cadmium ion Source: TAIR
  • response to salt stress Source: TAIR
  • response to zinc ion Source: TAIR

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

BioCyciARA:AT5G03630-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Monodehydroascorbate reductase 21 Publication (EC:1.6.5.4Curated)
Short name:
AtMDAR21 Publication
Gene namesi
Name:MDAR21 Publication
Ordered Locus Names:At5g03630Imported
ORF Names:F17C15.50Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G03630.
TAIRilocus:2144588. AT5G03630.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002091391 – 435Monodehydroascorbate reductase 2Add BLAST435

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei417PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ93WJ8.
PRIDEiQ93WJ8.

PTM databases

iPTMnetiQ93WJ8.

Expressioni

Inductioni

Down-regulated by atrazine.1 Publication

Gene expression databases

GenevisibleiQ93WJ8. AT.

Interactioni

Protein-protein interaction databases

IntActiQ93WJ8. 1 interactor.
STRINGi3702.AT5G03630.1.

Structurei

3D structure databases

ProteinModelPortaliQ93WJ8.
SMRiQ93WJ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiKOG1336. Eukaryota.
COG0446. LUCA.
HOGENOMiHOG000276711.
InParanoidiQ93WJ8.
KOiK08232.
OMAiYGLVEPA.
OrthoDBiEOG09360BD1.
PhylomeDBiQ93WJ8.

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
3.50.50.60. 3 hits.
InterProiView protein in InterPro
IPR036188. FAD/NAD-bd_sf.
IPR023753. FAD/NAD-binding_dom.
IPR016156. FAD/NAD-linked_Rdtase_dimer_sf.
PfamiView protein in Pfam
PF07992. Pyr_redox_2. 1 hit.
SUPFAMiSSF51905. SSF51905. 1 hit.
SSF55424. SSF55424. 1 hit.

Sequencei

Sequence statusi: Complete.

Q93WJ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEEKSFKYV IVGGGVAAGY AAREFFNQGV KPGELAIISR EQVPPYERPA
60 70 80 90 100
LSKGYIHLEN KATLPNFYVA AGIGGERQFP QWYKEKGIEL ILGTEIVKAD
110 120 130 140 150
LAAKTLVSGT GQVFKYQTLL AATGSSVIRL SDFGVPGADA KNIFYLRELE
160 170 180 190 200
DADYLAYAME TKEKGKAVVV GGGYIGLELG AALKANNLDV TMVYPEPWCM
210 220 230 240 250
PRLFTAGIAS FYEGYYANKG INIVKGTVAS GFTTNSNGEV TEVKLKDGRT
260 270 280 290 300
LEADIVIVGV GGRPIISLFK DQVEEEKGGL KTDGFFKTSL PDVYAIGDVA
310 320 330 340 350
TFPMKLYNEM RRVEHVDHAR KSAEQAVKAI KAAEEGNSIP EYDYLPYFYS
360 370 380 390 400
RAFDLSWQFY GDNVGESVLF GDNDPESPKP KFGSYWIKER KVVGAFLEGG
410 420 430
SPEENNAIAK LARAQPSVES LEVLSKEGLS FATNI
Length:435
Mass (Da):47,480
Last modified:December 1, 2001 - v1
Checksum:i1724F290D1EC43F7
GO

Sequence cautioni

The sequence CAB82928 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti390R → G in AAM64868 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL162506 Genomic DNA. Translation: CAB82928.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED90635.1.
AF428317 mRNA. Translation: AAL16247.1.
AY057628 mRNA. Translation: AAL15259.1.
AY142000 mRNA. Translation: AAM98264.1.
AY087318 mRNA. Translation: AAM64868.1.
AK227165 mRNA. Translation: BAE99207.1.
PIRiT48390.
RefSeqiNP_568125.1. NM_120444.5.
UniGeneiAt.33250.
At.4763.

Genome annotation databases

EnsemblPlantsiAT5G03630.1; AT5G03630.1; AT5G03630.
GeneIDi831774.
GrameneiAT5G03630.1; AT5G03630.1; AT5G03630.
KEGGiath:AT5G03630.

Similar proteinsi

Entry informationi

Entry nameiMDAR2_ARATH
AccessioniPrimary (citable) accession number: Q93WJ8
Secondary accession number(s): Q0WUJ1, Q8LBB1, Q9LZS5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: December 1, 2001
Last modified: November 22, 2017
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families